9TH EUROPEAN AIDS CONFERENCE (EACS) 1st EACS RESISTANCE & PHARMACOLOGY WORKSHOP October 25 - 29, 2003 Warsaw, Poland

Background: The question of laboratory criteria of a high risk of congenital CMV-infection, still remains undetermined.

Objective: To determine significance of DNA CMV detection in saliva, urine and/or blood of pregnant HIV- infected women as predictive markers of congenital CMV-infection.

Methods: Since 2001 to 2002 we have kept under observation 130 pregnant HIV-infected women and their newborn babies. Medical examination of a pregnant woman and her newborn baby included CMV DNA detection in saliva, urine and peripheral blood leukocytes (PCR-test). The mothers were examined twice during pregnancy. The newborn underwent examination at the age of 5 days.

Results: CMV DNA was detected in saliva of 24,6% of pregnant women, in urine and blood - of 18,5 % and 9,2% respectively. Antenatal infection of foetus by CMV was proved in 11 cases (8,5%). Sensitivity and specificity of CMV DNA presence in saliva of a pregnant woman as predictive marker of congenital CMV amounted to 95,6 % and 21,9 %; CMV DNA in urine - 96,2 % and 29,2 %; CMV DNA in blood - 96,6 % and 58,3 % respectively.

Conclusion: High frequency of active CMVI is characteristic of pregnant HIV-infected women (CMV DNA was detected in blood leukocytes of 9,2% of examined patients). Intrauterine infection of foetus by CMV happens more often (8,5 % of cases) if a pregnant woman is HIV-infected. CMV DNA detected in blood of pregnant women is the most sensitive and specific marker of a high risk of congenital CMV- infection.

Presenting Author: PHD Vasiliy Shakhgildian, scientific researcher, 8 ulitsa Sokolinoy Gori, 15-2, 105275, Moscow, Russian Federation, Phone: 007 095 366 05 18

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