Eighth International Congress on Drug Therapy in HIV Infection Glasgow, UK - 12-16 November 2006

Int Cong Drug Therapy HIV 2006 Nov 12-16;8:Abstract No. PL9.6

G Garrabou, O Coll, M Durban, S Lopez, R Vidal, A Suy, S Hernandez, F Cardellach, D Mataro, O Miro
Mitochodrial Research Laboratory, IDIBAPS, Hospital Clinic of Barcelona, Barcelona, Catalonia, Spain; Obstetrics Department, Hospital Clinic of Barcelona, Barcelona, Catalonia, Spain; Clínica Eugin, Barcelona, Catalonia, Spain

PURPOSE OF THE STUDY: Supported by Fundació la Marató de TV3 (02/0210), Redes de Investigación en Mitocondrias (V2003-REDC06E-0) y SIDA (Redg 173) and Suports a Grups de Recerca (2001/SGR/00379).

We have reported that HIV-infected women under antiretroviral treatment (ARVT) undergoing in vitro fertilization have a lower pregnancy rate than non-infected controls which depends on oocyte-related factors (1). We hypothesize that mitochondrial toxicity could be the underlying mechanism of such disturbance.

METHODS: We studied 19 and 11 previously frozen oocytes from 14 and 10 infertile HIV-infected and uninfected women, respectively, obtained after oocyte retrieval in vitro fertilization cycles. All of them had no further therapeutical value (were on metaphase I or non-fertilized) and all patients gave permission to be studied. MtDNA extraction and quantification by quantitative real-time PCR were carried out in each individual oocyte.

SUMMARY OF RESULTS: Mean age was 36 years for both groups. At inclusion HIV+ women had been on ARVT for 96±56 months, all were receiving NRTIs, had undetectable HIV-viral load and their mean CD4+ count was 770±296 cells/mm³. Oocytes from HIV-infected women had significantly lower mtDNA content than non-infected controls (88±51 vs. 165±76, 47% depletion, p=0.002). Such difference was better observed in oocytes on metaphase I (94±51 vs. 183±32, 49 % depletion, p=0.005) than in non-fertilized oocytes (83±59 vs. 149±101, 44% depletion, p=0.19). No significant correlation was found between mtDNA oocyte content of HIV-infected women and accumulative doses of ARV or their immunological status.

CONCLUSIONS: Oocytes of infertile HIV-infected ARV-treated women show decreased mtDNA and this could explain their poor reproductive outcome.

(1) Coll et al. “Decreased pregnancy rate after in-vitro fertilization in HIV-infected women receiving HAART.” AIDS. 2006 Jan 2;20(1):121-3.

Plenary Session: Adverse Events I

2006-11-12
PL9.6

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