1st International AIDS Conference Atlanta, Georgia, U.S.A. - April 14-17, 1985

Int Conf AIDS 1985 Apr 14-17; 1:22 (abstract no. S3C)

Mark Muesing, Douglas Smith, C Cabradillo, Charles Benton, Laurence Lasky, Daniel Capon
Genentech, Inc., South San Francisco, California and CDC, Atlanta, Georgia.

The 9,213 nucleotide structure of the AIDS/lymphadenopathy virus has been determined for one of the integrated proviruses present in the H9/HTLV-III cell line and for a complete set of overlapping cDNAS representing the viral RNA of distinguishable isolate(s) also present in H9/HTLV-III cells. These results demonstrate that the virus is not related by nucleotide homology to previously characterized animal and human retroviruses and that different virus isolates display significant genetic heterogeneity. Blot hybridization experiments reveal the apparent accumulation of several hundred copies of unintegrated linear viral DNA during acute infection of peripheral lymphocytes with LAV, an observation previously made for cytopathic avian retroviruses, while in chronically infected H9/HTLV-III cells, there is little evidence for the persistence of linear unintegrated DNA. Together with an analysis of the two major classes of spliced, subgenomic viral RNAs, these studies provide a detailed picture of the structure and processing of the gag, pol and Any gene products, and predict a novel gene at the 3' end of the genome unrelated to the X genes of HTLV-1 and -II.

850414
S3C

Copyright © 1985 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.