1st International AIDS Conference Atlanta, Georgia, U.S.A. - April 14-17, 1985

Int Conf AIDS. 1985 Apr 14-17;1:25 (abstract no. S6B)

V.S. Kalyanaraman, JP Getchell, TL Chorba, RB Ramsey, R Narayanan, DP Francis
CDC, Atlanta, Georgia.

We have characterized a human T-lymphotropic virus which was isolated from the cultured T-lymphocytes of a hemophilia patient with AIDS. This virus isolate was designated HTLV-III/LAV (CDC 451). By electron microscopic examination this virus is very similar to HTLV-III and LAV. In competition radioimmunoassays HTLV-I and II did not compete with the major core protein, P24, of this isolate, whereas HTLV-III and LAV competed fully. These results indicate that different AIDS isolates are closely related. Myristic acid labeling of HTLV-I, II and III/LAV (CDC 451) shows the incorporation of the label in PI9, P21, and P18, respectively, of the 3 viruses. These are therefore the amino-terminal proteins of the gag gene of the 3 viruses. A specific and sensitive Enzyme Linked Immunosorbent Assay (ELISA) detected antibodies to the CDC 451 strain in 97% of AIDS patients, in 95% of patients with lymphadenopathy syndrome, and in 1% of random donors and normal controls. Western blot analysis of the positive human sera revealed that the major reactivity was directed against proteins P41, P35, P24 and P18 of the CDC 451 isolate.

850414
S6B

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