1st International AIDS Conference Atlanta, Georgia, U.S.A. - April 14-17, 1985

Int Conf AIDS. 1985 Apr 14-17;1:27 Abstract No. S9B

Steven Benn, T Folks, A Rabson, M Lightfoote, MD Hogganm, M Martinet

A continuous lymphocytic leukemia cell line (A3.01) with a Leu 3+, Leu 8+ phenotype has been used to prepare high-titered stocks of the lymphadenopathy virus (LAV). The A3.01 line was also used for the isolation of full-length (10 kb) molecular clones of the LAV proviral DNA. Subgenomic segments have been used in Southern and Northern blot hybridization experiments to monitor a) the kinetics of viral DNA replication and b) the appearance of different species of viral RNA.

A3.01 cells are exquisitely sensitive to LAV; greater than 95% are killed by the late phase of viral infection. Coincident with the disappearance of the Leu 3+ cells, Leu 3- cells emerge that produce no detectable virus as monitored by reverse transcriptase activity. Western blot analysis of the Leu 3- cell lysates reveals the presence of viral specific proteins. Following the treatment with iododeoxyuridine, a lymphotrophic retrovirus was detected from the Leu 3- surviving cells. Nucleic acid in the uninduced and induced cells could be identified using specific subgenomic viral DNA probes. The population of Leu 3- cells that appears late in infection thus represents a model of human cytopathic retrovirus latency.

850414
S9B

Copyright © 1985 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.