1st International AIDS Conference Atlanta, Georgia, U.S.A. - April 14-17, 1985

Int Conf AIDS. 1985 Apr 14-17;1:28 Abstract No. S9D

J Steven Mcdougal, SP Cort, JKA Nicholson, D Cross, A Mahle, J Sligh

HTLV-III/LAV replication (detected by cytoplasmic immunofluorescence, release of viral antigen, and particulate reverse transcriptase activity) was monitored in the following cultures of normal human mononuclear cells (MNC) , phytohemagglutinin (PHA) stimulated and unstimulated MNC cultured with and without interleukin-2 (IL-2) and fetal calf serum or autologous serum (8 combinations); MNC that had been irradiated (2000R) 3 days after PHA Stimulation, residual MNC recovered12 days after initial HTLV-III/ LAV infection and restimulated (or not restimulated) with PHA, separated T cell, B cell plus monocyte, and monocyte populations, and both positively and negatively selected T4 and T8 T cells. Optimal virus replication occurs in PHA-activated T4 T cells cultured with IL-2. Virus replication occurs in irradiated MNC if these cells are PHA stimulated prior to irradiation. Results in macroculture with separated cell populations were confirmed in a microculture infectivity assay system under conditions of both constant cell concentration with limiting virus input and constant virus inoculum with limiting cell input. Although we could not directly demonstrate virus replication in monocytes, these cells inoculated with HTLV-III/LAV are capable of transmitting virus Lo PHA-stimulated MNC.

850414
S9D

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