1st International AIDS Conference Atlanta, Georgia, U.S.A. - April 14-17, 1985

Int Conf AIDS. 1985 Apr 14-17;1:28 Abstract No. S9F

Anne J Bodner, DS Watson, DA Brusini, AE Williams, RR Redfield, NA Stombaugh

An Enzyme Linked Immunosorbent Assay (ELISA) has been developed to screen blood products for antibodies to Human T-Cell Lymphotropic Virus-III (HTLV-III), the virus believed to be the causative agent of Acquired Immune Deficiency Syndrome (AIDS). During preclinical evaluation of this ELISA methodology, 23 of 23 sera of known AIDS or AIDS Related Complex (ARC) patients were determined to contain IITLV-III antibodies using this system. 68 of 69 subjects at low risk for AIDS tested negative. The detection system utilizes detergent disrupted viral proteins, adsorbed onto a microtiter plate. Following incubation of human serum or plasma with the viral antigens, bound HTLV-III antibodies are detected with alkaline phosphatase conjugated anti-human immuno-globulin. Relative quantities of bound antibody are measured through reaction 'with p-nitrophenylphosphate. Data will be presented showing good correlation between this ELISA assay and data from Western Blot analyses. The ELISA test is currently being clinically evaluated at several blood research centers in the United States.

850414
S9F

Copyright © 1985 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.