1st International AIDS Conference Atlanta, Georgia, U.S.A. - April 14-17, 1985

Int Conf AIDS. 1985 Apr 14-17;1:28 Abstract No. S9G

Steve S Alexander Jr, C Tal, RL Ting, AJ Corrigan, AJ Bodner, DW Julien

A Western Blot system using Biotin-Avidin detection has been utilized to study the composition of viral proteins from Human T-Cell Lymphotropic Virus-III (HTLV-III). Purified HTLV-III viral proteins are electrophoresed on 12% SDS slab gels and electro-transferred onto nitro cellulose sheets. These Western Blots are then cut into 0.5 cm strips and reacted with .either monoclonal antibodies to specific viral proteins or with human sera or plasma known to contain HTLV-III antibodies. Multiple viral proteins (MW l5K, 24K, 41K, 55K, 64K, 130K, and 160K) can be visualized with most sera from Acquired Immune Deficiency Syndrome (AIDS) or AIDS Related Complex (ARC) patients. At higher antibody concentrations, as many as 5 distinct bands can be visualized in the 39-43K range. The sensitivity of this Biotin-Avidin system is sufficient to detect the gag protein p24 in high titer sera diluted to 1: 500,000. This sensitivity is comparable to an ELISA system currently under development for the screening of blood products for HTLV-III antibodies.

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S9G

Copyright © 1985 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.