4th International AIDS Conference Stockholm, Sweden. — June 12-16, 1988

Int Conf AIDS. 1988 Jun 12-16;4:1.115 (abstract no. 1010)

Sylvia Crush-Stanton, Bonnie Swerdlow and Michael L. Berman
Bionetics Research, Inc., Rockville, Maryland, U.S.A.

Sonicated HIV1_RF¹ gag and pol DNA was used to create a subgenomic library in a previously described λ phage expression vector². Recombinant clones were screened for expression of antigenic determinants with a pool of several patients' serum. Individual clones were subsequently characterized with anti-p24 mouse monoclonal antibodies. The recombinant proteins express different epitopes as defined by monoclonal antibodies allowing mapping of the antigenic determinants. Data will be presented showing differing monoclonal reactivity on clones that produce stable recombinant proteins in E. coli. The recombinant antigens may prove useful for in vitro diagnostics. The approach used to produce these clones is generally applicable to the expression of any stable recombinant antigen and its subfragments for diagnostic, vaccine and pharmaceutical purposes.

^l Kindly provided by Beatrice Hahn, Flossie Wong-Staal and R. Gallo.

² Meissner, William P. Sisk, M. L. Berman. "Bacteriophage lambda cloning system for the construction of directional cDNA libraries" Proc Natl Acad Sci U S A. 1987 Jun;84(12):4171-5.

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Copyright © 1988 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.