4th International AIDS Conference Stockholm, Sweden. — June 12-16, 1988

Int Conf AIDS. 1988 Jun 12-16;4:1.115 (abstract no. 1012)

Teresa CABEZON¹, Tineke RUTGERS¹, Martine DESCURIEUX¹, Jacqueline COGNIAUX², Ralph BIEMANS¹ and Michel DE WILDE^1
1Dept. of Mol. and Cell. Biology, Smith Kline-RIT, 1330 Rixensart, Belgium; ²Institut Pasteur du Brabant rue du Remorqueur 28 1040 Brussels, Belgium

Yeast shuttle vectors harboring the nucleotide sequences coding for the p17 mature HIV protein or for a fusion p17-p24 protein were constructed and used to transform yeast strains. The yeast cells transformed with those plasmids express p17 related proteins that immunoreact with specific antibodies and with HIV positive human sera. Moreover the 'yeast product is a post- translationally modified protein that carries a myristoyl fatty acid residue attached to N-terminal glycine as has been found to be the case for the viral p17. The behaviour and subcellular localization of the r-p17 related protein in yeast has been studied.

The recombinant constructs were derived from the BH10 clone of HIV, kindly' provided by Dr Wong-Staal.

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