Table of Contents - 5th IAC 1989

Section A
Épidémiologie et santé publique
Epidemiology and Public Health

Études séro-épidémiologiques: États Unis
Seroepidemiologic Studies: United States

HIV-seroprevalence among patients of a West-German inner-city general practice.: Int Conf AIDS 1989 Jun 4-9; 5:161 (abstract no. A.501); Zeschel A, Meyer-Glauner W; 1.) To screen selected subpopulations among patients of a general practice as an economical alternative to untargeted mass-screening. 2.) Early detection of HIV-infection among high- and low-risk groups. 3.) To counsel and treat these otherwise unrecognized HIV-carriers as a contribution to the containment
HIV infection related to the use of contaminated material in commercial plasma donors.: Int Conf AIDS 1989 Jun 4-9; 5:161 (abstract no. A.502); Clotet B, Carles J, Tural C, Sirera G, Dominguez M, Tor J, Foz M; To identify the risk factors for AIDS reported by 33 HIV seropositive paid blood/plasma donors. MATERIAL: Patients who donated blood in private centers in a retributed manner and were found HIV seropositive. In all cases HIV antibodies were evaluated by ELISA and Western Blot methods. In 11 out of 3
Risk of HIV transmission to health care workers and comparison with viral hepatitides.: Int Conf AIDS 1989 Jun 4-9; 5:161 (abstract no. A.503); Rastrelli M, Ferrazzi D, Vigo B, Giannelli F; To assess the risk of nosocomial transmission of HIV in comparison with viral hepatitides and record the effects of adopting further protective and educational means. During the period June 1986-December 1988, all health care workers accidentally exposed to HIV in hospital had entered the study and
Monitoring prevalence of HIV infection and epidemiological patterns through laboratory data.: Int Conf AIDS 1989 Jun 4-9; 5:161 (abstract no. A.504); Casabona J, Bosch A, Tor J, Segura A; 1.- To estimate the prevalence of HIV infection among people who request the test in Catalonia. 2.- To describe the demographic and behavioral characteristics of this population. All laboratories performing HIV test within the Public Health System are used. The total number of test and their result
Prevalence of infection with human immunodeficiency virus among drug users in Puerto Rico.: Int Conf AIDS 1989 Jun 4-9; 5:161 (abstract no. A.505); Gorbea HF, Garcia ME; To study the prevalence of HIV infection among drug users (DU) in a drug treatment program in Puerto Rico (PR). DU voluntarily agreed to participate in the study. Information concerning drug use, sexual and medical history was obtained. A screening test (EIA) and supplemental test (Western blot or I
International networking of HIV public health services.: Int Conf AIDS 1989 Jun 4-9; 5:161 (abstract no. A.506); Daniell FD, Skelly R, Sanderson R, Novak D, Cunnion S; To expand public health capabilities against the HIV epidemic, a networking of existing U.S. Navy preventive medicine programs and approaches was initiated during 1988, utilizing an STD model. International networking involves utilization of services provided at a medical center caring for HIV-infec
MEN MEET WOMEN: DIFFERENTIAL RISK-REDUCING NEEDS AND TACTICS IN ENCOUNTERS BETWEEN PEOPLE DRAWN FROM THE GENERAL POPULATION POOL: Int Conf AIDS 1989 Jun 4-9; 5:162 (abstract no. A.507); Shtarkshall RA, Awerbuch T; The implications of these findings are profound when formulating the objectives and concepts of educational interventions among sexually active heterosexuals. While condoms are used by men, it is mainly in the interest of women that men should use condoms. Thus it may be essential to introduce altruistic "cooperative" motives into educational interventions to supplement the "egoistic" one of "safe sex." The introduction of "responsible sex" and its implications are considered.
"GROUPS AT RISK" AND "RISK BEHAVIORS": THE NEED TO UTILIZE BOTH CONCEPTS IN DESCRIBING TARGET POPULATIONS FOR EDUCATIONAL INTERVENTIONS AND DEFINING THEIR OBJECTIVES: Int Conf AIDS 1989 Jun 4-9; 5:162 (abstract no. A.508); Shtarkshall RA; Groups A and B approximate homosexuals/bisexuals and adolescents in Israel, respectively, while group C may represent the sub-population of hemophiliacs after the introduction of sterilized factors. It is argued that from the point of view of public health, health planning, and the design of educational interventions, both concepts are essential, since educational interventions for a group at future risk should differ from those for a group at immediate risk not only because of the characteristics of the group but also because of the characteristics of the risk. “Groups at risk” is therefore a useful and necessary term.
Surveillance of HIV-2 infections by ELISA using HIV-1 and HIV-2 specific synthetic peptides.: Int Conf AIDS 1989 Jun 4-9; 5:162 (abstract no. A.509); Leinikki P, Hakulinen J, Kantanen ML, Brummer-Korvenkontio H; To screen for HIV-2 cases in Finland and to evaluate a new test employing HIV-1 and HIV-2 specific synthetic peptides as antigens. Serum samples sent for routine HIV-testing from healthy individuals and HIV risk groups were tested by an ELISA with either a combination of, or individual HIV-1 or HIV-
HTLV seroprevalence in a group of HIV positive Italian subjects (in majority intravenous drug abusers) - proemial data.: Int Conf AIDS 1989 Jun 4-9; 5:162 (abstract no. A.510); Lopalco L, Ciccomascolo F, Lanza P, Berretta A, Bini A, Sabbatani S; Italy; AIMS: We detected the presence of specific HTLVI antibodies in HIV1 positive subjects to evaluate (1) the percentage of double infection (2) if the subjects with double infection presented an evolutive pattern. The presence of specific HTLVI antibodies was tested by Elisa method in 240 HIV1 positive subjects (
Incidence of HBV markers and HIV antibodies in blood donors in Luanda and Dundo, People's Republic of Angola.: Int Conf AIDS 1989 Jun 4-9; 5:162 (abstract no. A.511); Lourenco MH, Cohen T, Octavio F, Santos-Ferreira MO, Matos-Almeida MJ, Azevedo Pereira JM; To determine the prevalence of HIV 1 and HIV 2 infection and hepatitis B markers among a cohort of blood donors in two regions of Angola . The incidence of HBV markers and HIV antibodies was evaluated in 124 blood donors living in Luanda (74) and Dundo (50). Serological tests for detection of HIV an
Analysis of a screening program for HIV1 and HIV2 infection in women of reproductive age.: Int Conf AIDS 1989 Jun 4-9; 5:162 (abstract no. A.512); Victorino R, Teles LC, Ferreira MO, Guerreiro D, Lourenco MH; 1 - To study the prevalence of antibodies to HIV1 and HIV2 in a population of women attending a Family Planning Clinic (FPC) and a antenatal clinic (AC) serving a urban population of 30.000 people where about 10% are of African origin. 2 - Devise a protocol of a study which would maintain characteristics of
Stochastic models illustrate vividly the connection between behavior and risks for HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:163 (abstract no. A.513); Gonzalez JJ, Myrtveit M, Vavik L; ASA, Arendal, Norway; To use the power of modern Personal Computers to display graphically the connection between behavior and risk for HIV infection. A stochastical simulation model takes into account behavior parameters in a hypothetical risk environment. The correlation between behavior patterns and risk for HIV infec
HIV screening efforts in a low-risk population.: Int Conf AIDS 1989 Jun 4-9; 5:163 (abstract no. A.514); Magruder CD, Sanchez JL, Huffman P, Lewis VM, Quattromani FL; To determine the prevalence of HIV antibody seropositivity in a low-risk population. The U.S. Army requires HIV testing at least biennially for individuals on active duty. Blood donors are also routinely screened for HIV infection. During the past year, inpatients and some outpatients have received
Is sexual promiscuity a risk factor for HHV6 in East Africa?: Int Conf AIDS 1989 Jun 4-9; 5:163 (abstract no. A.515); Fox E, Constantine N, Abbatte EA; To investigate if the B-cell lymphotropic virus HHV6 shares the sexual transmission route of HIV in East Africa. 200 sera belonging to 4 groups of individuals from the East African city of Djibouti were tested for antibodies against HHV6. Study groups comprised sexually promiscuous individuals at hi
A prospective study of HIV infection in former drug users: a 4-year study of 114 patients.: Int Conf AIDS 1989 Jun 4-9; 5:163 (abstract no. A.516); Gringeri A, Rumi MG, Colombo M, Zanetti AR, Romeo R, Mannucci PM; To assess whether or not stopping drugs has any beneficial effect on the course of HIV infection, inactive drug users living in the community of San Patrignano ( Italy ) were prospectively followed. These patients voluntarily enrolled into a rehabilitation program based on agricultural, handicraft a
Accidental exposures to HIV-positive blood among health-care workers in 2 Swedish hospitals.: Int Conf AIDS 1989 Jun 4-9; 5:163 (abstract no. A.518); Jorbeck H, Marland M, Steinkeller E; To document circumstances, frequency and outcome of accidental exposures to HIV-positive blood and bloody body fluids among health-care workers. This prospective study was started in January 1, 1985. It includes health-care workers in 2 Swedish hospitals giving medical care for HIV-infected persons
National study of HIV seroprevalence in Native Americans.: Int Conf AIDS 1989 Jun 4-9; 5:164 (abstract no. A.519); Conway GA, Hooper EY, Helgerson SD, Petersen LR, Leach R, Johannes P; To assess existing levels and future trends of HIV infection among American Indians and Alaska Natives in the United States . Following approval from tribal authorities, blinded seroprevalence testing will be conducted on sera obtained for syphilis screening from prenatal patients, patients undergoi
Detection of HIV antibodies in dental school at the Buenos Aires University.: Int Conf AIDS 1989 Jun 4-9; 5:164 (abstract no. A.520); Crespo D, Sabelli C, Trica A, Brizuela L, Ramella M, Muchinik G; The aim of this work was to determine the seroprevalence of HIV infection in dental professionals as they represent an important group to study for occupational risk of HIV infection. A total of 617 subjects were included in this study. 400 were first year students at the School of Dentistry, 112 pr
Seroprevalence of HIV antibody in a citywide sample of emergency room patients.: Int Conf AIDS 1989 Jun 4-9; 5:164 (abstract no. A.521); Modesitt S, Jui J, Stevens P, Hulman S, Shriver J, Fleming D; To determine prevalence of HIV antibody in a citywide population of patients seeking emergency room (ER) care. Seven hospital-based emergency rooms providing 90% of Portland s emergency services collaborated to simultaneously measure HIV seroprevalence in their combined patient population. During t
Sexual mixing models with like-with-like preference.: Int Conf AIDS 1989 Jun 4-9; 5:164 (abstract no. A.522); Blythe S, Castillo-Chavez C; To develop an alternative to proportionate mixing in sexually transmitted disease models. Starting from the three fundamental constraints on a mixing function (it must be positive, normalized, and conserve the number of partnerships), a new mixing model can be constructed by finding an appropriate t
Acceptance of HIV-antibody screening and prevalence of antibodies against HIV at a STD clinic.: Int Conf AIDS 1989 Jun 4-9; 5:164 (abstract no. A.523); Kvinesdal BB, Worm AM; During periods of three weeks repeated every third month from October 1987 to November 1988, all persons attending a STD clinic in Copenhagen for examination were offered a blood test for HIV-antibodies. The test could be performed anonymously or not. Data concerning sexual orientation, prostitution, drug abuse and sex
The influence of changes in sexual behaviour on the transmission dynamics of HIV-1 in the UK.: Int Conf AIDS 1989 Jun 4-9; 5:164 (abstract no. A.524); Gupta S, Blythe S, Anderson RM; To assess the consequences of changes in sexual behaviour on the transmission dynamics of HIV-1 with reference to the male homosexual community in the United Kingdom . The distribution of sexual activity (rate of acquiring new partners) and transmission probabilities (mirroring the adoption of safe
HIV seropositivity in IVDU Vietnam combat veterans.: Int Conf AIDS 1989 Jun 4-9; 5:165 (abstract no. A.525); Kocurek K, Bacchetti P, Gorter R, Meakin R, Moss AR; To determine the rate of HIV infection in heterosexual military veterans who use intravenous drugs and are seen in methadone treatment facilities. Heterosexual IVDUs attending methadone treatment facilities in San Francisco between February and December 1988 were recruited, screened for military ser
Completeness and accuracy of risk factor information in a general population surveillance of HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:165 (abstract no. A.526); Perucci CA, Arca M, Carboni A, Orchi N, Spadea T; To detect and quantify the biases due to missing information about risk factor in data from a general population surveillance of HIV. Anonymous notification of every confirmed anti-HIV positivity, including available information on risk factor, has been collected by the Latium R.E.U. from local labo
Temporal trends of HIV prevalence in blood donors and use of blood banks as testing sites in Latium (Italy).: Int Conf AIDS 1989 Jun 4-9; 5:165 (abstract no. A.527); Perucci CA, Arca M, Abeni D, Chierchini P; To evaluate changes over time in number and characteristics of blood donors and their effect on observed prevalence trends of HIV infection and to evaluate the effectiveness of screening programmes. In the Latium region (about 5,000,000 inhab.) screening of every donated blood unit has been mandator
HIV seroprevalence in Hispanic amnesty applicants at a sexually transmitted diseases (STD) clinic, Los Angeles, California, 1988.: Int Conf AIDS 1989 Jun 4-9; 5:165 (abstract no. A.528); Nahlen B, Kerndt P, Ford W, Johnson M, Waterman S; To estimate and compare HIV antibody (Ab) seroprevalence in undocumented Hispanics seeking legal amnesty with that of other Hispanics attending the same STD clinic. Amnesty applicants must prove residence in the USA since Jan. 1, 1982, and undergo HIVAb testing. Repeat ELISA positives are confirmed
Seroprevalence of HIV antibody in IV drug users and other patients in a Connecticut hospital.: Int Conf AIDS 1989 Jun 4-9; 5:165 (abstract no. A.529); Lyons RW, Krisiunas E, Madden G, Rubinstien E, Smith C, Dauphinais R; To examine the demographics of HIV antibody seroprevalence at a 592 bed community teaching hospital in the northeastern United States in 1988. Sex, race, age, and risk-group information was obtained by the laboratory from the ordering physician on all HIV antibody tests done. Patient identifiers wer
The epidemiology of AIDS in Maryland, 1981 - 1987.: Int Conf AIDS 1989 Jun 4-9; 5:165 (abstract no. A.530); Horman J, Hamidi C; Describe the epidemiology of AIDS in Maryland for the years 1981 through 1987. Case reports completed as part of the Maryland AIDS Reporting System were analyzed. A total of 1,017 nonincarcerated adult AIDS cases was reported during this 7 year period. The greatest proportion of cases occur
[3 year surveillance of T4 lymphocytes and B2 microglobulin in a cohort of 166 subjects infected with HIV]: Int Conf AIDS 1989 Jun 4-9; 5:166 (abstract no. A.531); Brugnetti A, Negre M, Pesce A, Fuzibet JG, Quaranta JF, Dujardin P, Cassuto JP
Screening for HIV infection among IV drug users in the prisons of Brussels.: Int Conf AIDS 1989 Jun 4-9; 5:166 (abstract no. A.532); Donot A, Verhoeven-Lespagnard L, Lumer G; To monitor the epidemic of HIV infection among IV drug users (IVDU) in the Brussels area through screening of the inmates of Brussels prisons and, at the same time, monitor the effect of information on the behavior of the IVDU population. Since May 1985, an information campaign on AIDS was launched
Federal support of state and local HIV prevention programs in the United States.: Int Conf AIDS 1989 Jun 4-9; 5:166 (abstract no. A.533); Bowen GS, West GR, Kirby J, Cates W, Hinman AR; To provide financial and technical assistance to State and local health departments for the prevention of HIV transmission in the U.S. CDC is the lead federal agency in the U.S. Government for development, implementation, and evaluation of national efforts to prevent transmission of HIV and to contr
HTLV-I/II INFECTION IN HIGH-RISK GROUPS IN SPAIN: Int Conf AIDS 1989 Jun 4-9; 5:166 (abstract no. A.534); Genesca J, Viladomiu L, Talavera JC, Shih J, Alter H, Esteban JI; 1) The prevalence of HTLV-I/II infection in HIV infected groups in Spain is very low; 2) Additional confirmatory testing for HTLV-I/II infection is absolutely necessary; 3) The recombinant p21E WB assay might be a useful confirmatory technique for HTLV-I/II infection.
AN ANALYSIS OF AIDS IN BRITISH COLUMBIA: Int Conf AIDS 1989 Jun 4-9; 5:166 (abstract no. A.535); Rekart ML; Recently reported cases of AIDS in British Columbia are occurring more frequently as a result of heterosexual transmission. All other characteristics have remained stable over time. The incidence of AIDS has slowed considerably.
Evolution towards AIDS in a group of Italian intravenous drug abusers (IDA) with PGL.: Int Conf AIDS 1989 Jun 4-9; 5:166 (abstract no. A.536); Luzzati R, Giraldi D, Malena M, Mazzi R, Ciaffoni S, Roata C; To evaluate the 3-year progression rate to AIDS in a group of IDA with PGL in Verona (Italy). One hundred and thirtytwo IDA (mean age 27 +/- 6 years) with PGL were enrolled in a prospective study between July 1984 and June 1986. Except for PGL, all patients were symptomless and had CD4+ lymphocyte
Impact of revised case definition on AIDS surveillance in Europe.: Int Conf AIDS 1989 Jun 4-9; 5:167 (abstract no. A.537); Ancelle-Park R, Couturier E, Brunet JB; WHO Collaborating Centre on AIDS, Paris, France; To determine the modalities of application and impact of the revised CDC/WHO AIDS case definition on surveillance in Europe. A survey was carried out among the 32 countries of the WHO European region investigating modalities of surveillance of AIDS and application of revised cases definition. RESULT
The incidence of glove puncture during Caesarian section and various potential solutions.: Int Conf AIDS 1989 Jun 4-9; 5:167 (abstract no. A.538); Smith JR, Grant JM; A study was performed to ascertain the incidence of glove puncture at lower uterine segment Caesarian section. The increasing incidence of Hepatitis B and HIV infection in women makes this of increasing importance to surgeons. The following results were obtained: TABULAR DATA, SEE ABSTRACT VOLUME. This trial shows the
[Modifications and geographical variations in transmission groups of HIV infections in France]: Int Conf AIDS 1989 Jun 4-9; 5:167 (abstract no. A.539); Jestin C; Ligue Francaise pour la Prevention des Maladies Infectieuses, France
HIV seropositive men infected with multiple CMV strains: a longitudinal study of the relationship to T-cell subsets.: Int Conf AIDS 1989 Jun 4-9; 5:167 (abstract no. A.540); Leach C, Detels R, Visscher B, Giorgi J, Cherry J; To investigate the relationship between serial infection with multiple CMV strains and T-cell subsets. We identified 56 asymptomatic HIV-1 seropositive homosexual men participating in a study of HIV-1 infection who excreted CMV from semen at two visits 5 to 9 months apart. CMV DNA was isolated from
Safer sex behavior reinforcement in public parks.: Int Conf AIDS 1989 Jun 4-9; 5:167 (abstract no. A.541); Lerro M, Freeman A, Valentine J, Haley CE; Reinforce safer sex behaviors among sexually active gay men. Health department staff learned of the high frequency of contact for sexual purposes among gay and bisexual men, particularly at one public park in a gay-identified neighborhood in Dallas. The health department employees prepared packets f
Prevention of perinatal transmission of HIV.: Int Conf AIDS 1989 Jun 4-9; 5:167 (abstract no. A.542); Berman S; To report progress to date by projects to prevent perinatal HIV transmission. Nine demonstration projects evaluate and facilitate the use of contraception and encourage risk reduction among women HIV-infected or at highest risk for infection. The projects, funded for $4.6 million per yr, ar
[HIV, syphilis and drug abuse]: Int Conf AIDS 1989 Jun 4-9; 5:168 (abstract no. A.543); Espinoza P, Loubeyre JL, Benamouzig R, Bouchard I; Etablissement d'Hospitalisation Public National de Fresnes, France
Detection of occlusion vs leak in condom rheological test.: Int Conf AIDS 1989 Jun 4-9; 5:168 (abstract no. A.544); Torres J, Anabitarte H, Usieto R, Noguera E; Comite Anti-SIDA, Barcelona, Spain; The condom is recommended and used as an efficient barrier against HIV and others. In practice, the film of a condom may present irregularities or leaks. This has been resolved efficiently with rigorous sampling plans for leak-detection tests, realized in all standards by conductometric or electronical test
Early course of HIV infection in 40 seroconverters.: Int Conf AIDS 1989 Jun 4-9; 5:168 (abstract no. A.545); Alessi E, Muratori S, Zerboni R, Cusini M, Carminati G, Mansi M; 1st Clinic of Dermatology, University of Milan, Milan, Italy; To study the natural history of HIV infection. Forty homosexuals, who became seropositive from December 1985 to January 1988, were followed from 11 to 36 months (mean 23 months). The mean time between the last negative and first positive tests was considered to be the time of infection. Clinical, im
Natural history of HIV infection in females seen at the Medical College of Virginia (MCV).: Int Conf AIDS 1989 Jun 4-9; 5:168 (abstract no. A.546); Berry A, Fortner C, Settle J, Kaplowitz L; Medical College of Virginia, Richmond, VA, USA; To describe the natural history of HIV infection in females seen at MCV. This study retrospectively compares the clinical course of 66 HIV infected women with 412 HIV infected men followed at MCV from 4/86 to 1/89. Females comprised 14% of the HIV infected population seen at MCV; 50 (77%) w
[Epidemiological profile of infections due to human immunodeficiency virus (HIV) in Estrie]: Int Conf AIDS 1989 Jun 4-9; 5:168 (abstract no. A.547); Duperval R, Boivin D, Marcoux JA, Lepine M, Saad WP; Universite de Sherbrooke, Sherbrooke, Quebec, Canada
Long term follow-up of homosexual men with persistent lymphadenopathy: attack rate for AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:168 (abstract no. A.548); Sepkowitz K, Gold JW, Campbell SW, Armstrong D; Memorial Sloan-Kettering Cancer Center (MSKCC), New York, NY, USA; To determine the attack rate for AIDS in a cohort of 93 homosexual men identified with lymphadenopathy between 1981-1983. Cases of AIDS occurring in a previously described cohort (Medicine 1985; 64: 203-213.) followed at MSKCC were documented. Patients lost to follow-up (LF) were traced. The attack
Lack of association between frequency of blood donation and seropositivity for HIV and other infections; an unexpected finding in Rio de Janeiro - Brazil.: Int Conf AIDS 1989 Jun 4-9; 5:169 (abstract no. A.549); Ramos Filho CF, Perez MA, Chain R, Martins FS, Jesus VC, Martins CN; Federal University of Rio de Janeiro, Rio de Janeiro (RJ) Brazil; Due to the importance of blood transmission in the diffusion of infectious disease in RJ we have performed this study in order to better understand blood transmitted disease and frequency of blood donation. 2,858 (non-paid) blood donors (18 to 60 years old) from the hemotherapeutic center of our hos
Seroepidemiology of human immunodeficiency virus type-I and viral hepatitis infection in New York City intravenous drug users (IVDUs).: Int Conf AIDS 1989 Jun 4-9; 5:169 (abstract no. A.550); Brown LS, Kreek MJ, Trepo C, Chu A, Ajuluchukwu D, Phillips R; Addiction Research and Treatment Corporation, Brooklyn, NY, USA; To examine the behaviors associated with HIV-1 and viral hepatitis infection in IVDUs enrolled in drug treatment. As a part of a larger HIV seroprevalence study in which a standardized questionnaire was administered, 50 subjects were recruited from patients enrolled in drug treatment in NYC in 1987.
Trends in AIDS incidence based on active surveillance in 12 medical school affiliated hospitals.: Int Conf AIDS 1989 Jun 4-9; 5:169 (abstract no. A.551); Lavenhar MA, Schneider DJ; UMDNJ-New Jersey Medical School, Newark, New Jersey, USA; To demonstrate how trends in AIDS incidence can be assessed utilizing data obtained through active surveillance in 12 hospitals affiliated with the New Jersey Medical School. Analyses will focus on descriptive methods for presenting data in tabular or graphic form. Survival analysis (with co-factors
[Coinfection with HIV-1 and HTLV-I]: Int Conf AIDS 1989 Jun 4-9; 5:169 (abstract no. A.552); Patey O, N'Go N, Dublanchet A, Lafaix C; Service des maladies infectieuses et tropicales, Villeneuve St Georges-France
Human immunodeficiency infection in the elderly: a retrospective analysis of 20 patients.: Int Conf AIDS 1989 Jun 4-9; 5:169 (abstract no. A.553); Thompson J, Kapila R, Tecson F; New Jersey Medical School and VA Hospital, East Orange, N.J., USA; From January 1985 to June 1988 twenty (20) patients over the age 60 years were diagnosed with HIV infection/AIDS according to CDC criteria group I-IV. Their ages ranged from 60-85 years with a mean of 66.1. The group included 18 men and 2 women. The risk factors for the men were Homosexuality, 6/18 (33%), IVDA 3/18 (15
HIV infection in judicial autopsy.: Int Conf AIDS 1989 Jun 4-9; 5:169 (abstract no. A.554); Barbieri DD, Buchala CM, Guidugli R, Lima LO; Centro de Regerencia e Treinamento da Secretaria de Estado da Saude de Sao Paulo, Brazil; The violent deaths, the suspects deaths and deaths with any delictous evidence must be investigated by a judicial autopsy. Considering the stigmatizing character of AIDS, the high incidence in homosexual and drug abusers and the high prevalence in prisons we expected that suicide, homicides and suspect deaths could occ
Estimates of the AIDS epidemic in Costa Rica, 1989-1993.: Int Conf AIDS 1989 Jun 4-9; 5:170 (abstract no. A.555); Mata L, Valadez J, Marranghello L; INISA, University of Costa Rica, San Pedro, Costa Rica; To estimate the size and impact of the AIDS epidemic. Five-year projections were obtained by fitting onto four statistical models all cases from epidemic onset in 1984 through December 1988. The data best fitted the power curve. Linear regression, exponential and logarithmic curves produced
Retrovirus infection (HIV-1, HIV-2, HTLV-I) in asymptomatic West Africans living in Spain.: Int Conf AIDS 1989 Jun 4-9; 5:170 (abstract no. A.556); Soriano V, Tor J, Ribera A, Fernandez JL, Muga R, Foz M; Infectious Diseases and Blood Bank Units, Hospital de Badelona "Germans Trias: Pujol". Universitat Autonoma de Barcelona, Spain; It has been postulated an African origin of human T-lymphotropic retroviruses. While HIV-1 and HIV-2 produced AIDS, the HTLV-I may be the etiologic agent of a subacute myelopathy and an acute T lymphocytic leukemia. However, the worldwide spread of these viruses may occur by infected African persons which ha
[Evaluation of susceptibility to AIDS prevention in university students in Aix-Marseille]: Int Conf AIDS 1989 Jun 4-9; 5:170 (abstract no. A.557); Beresniak A, Enel P, Sambuc R, San Marco JL; Laboratoire de Sante Publique, Faculte de Medecine, Marseille, France
The HIV epidemic beyond the epicenter: "inner city" epidemiology in a predominantly white suburban population.: Int Conf AIDS 1989 Jun 4-9; 5:170 (abstract no. A.558); Shepp D, Kaplan M, Smith M, Muggeo C; Cornell Univ. Medical College, North Shore University Hospital, Manhasset, NY, USA; To characterize the epidemiology of HIV infection seen in a university-affiliated teaching hospital located in a suburban setting beyond the New York City limits in Long Island. Hospital and clinic records of the Div. of Infectious Disease were used to create a computerized data base. Referral patte
Regional differences in survival with AIDS among gay white males in the United States.: Int Conf AIDS 1989 Jun 4-9; 5:170 (abstract no. A.559); Piette J; Brown University, Providence, Rhode Island; USA; To examine geographic variation in survival with AIDS among gay white males in the United States . Survival curves are calculated using data from a census of 24,668 AIDS cases reported to the Centers for Disease Control by 10/3/88. Analyses are presented by region and controlling for the presence of
Possibilities of forecasting HIV spread in large populations. A modelling tool for decision makers.: Int Conf AIDS 1989 Jun 4-9; 5:170 (abstract no. A.560); Koch MG, Dorner D, Gonzalez JJ, L'ageStehr J, Davidsen P, VonWelck U; Vardcentralen, S-546 00 Karlsborg, Sweden; In comparison between different methods of forecasting the development of an epidemic, computer based simulation techniques are well established and show several advantages, among them high speed, flexibility, transparency, and reproducibility. The program AIDS Spread Simulations and Projections (ASSP) is a modelling t
Correlation of incubation period and progression to disease in HIV carriers.: Int Conf AIDS 1989 Jun 4-9; 5:171 (abstract no. A.561); Koch MG, Davidsen P, VonWelck U, Gonzalez JJ; Vardcentralen, S-546 00 Karlsborg, Sweden; Comparing about 50 different clinical follow-up studies from various regions in the world, we tried to define the progression rates of HIV infected persons to AIDS. The main problem is that different patient groups never are fully equivalent concerning important variables as their distribution into age and risk categor
HIV seroprevalence at Cook County Hospital, Chicago: a two-year follow-up.: Int Conf AIDS 1989 Jun 4-9; 5:171 (abstract no. A.562); Seibert D, Tsui P, Cohen M, Hamb A, Sherer R; Cook County Hospital AIDS Prevention Services, Cook County Jail. Chicago, Il, USA; To determine the prevalence of HIV infection among persons at risk of HIV infection at Cook County Hospital, Chicago, in 1987 and 1988, and to compare trends among groups at risk. From 1/1/88 to 12/31/88, 2,084 clients received HIV antibody counseling. Demographics: 42% women, 58% men; 69% black, 1
Women with AIDS: a continuing study 1988.: Int Conf AIDS 1989 Jun 4-9; 5:171 (abstract no. A.563); Kloser P, Bais P, Lynch A, Lombardo J, Kapila R; University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA; To describe the epidemiology and natural history of HIV infection in women in Newark, New Jersey. This is a continuation of our study of women with HIV disease started in 1980. The addition of 174 new cases in 1988 makes a total of 538 women seen with HIV infection at our facility. The wome
Survey of streetwalking prostitutes in New York City for anti-HIV-1 antibodies.: Int Conf AIDS 1989 Jun 4-9; 5:171 (abstract no. A.564); Wallace JI, Beatrice S; Foundation for Research on Sexually Transmitted Diseases, New York, NY, USA; To determine the rates of HIV-1 exposure in streetwalking prostitutes of New York. In 1985-1988 prostitutes working on the streets of New York City were tested for anti-HIV-1 antibodies. Blood was drawn on-site, and was tested for antibodies by ELISA with Western Blot confirmation. The women were qu
Effect of age on AIDS attack rate in hemophilics in California.: Int Conf AIDS 1989 Jun 4-9; 5:171 (abstract no. A.565); Gomperts E, Holman R, Jason J, Evatt B, Addiego J, Hartman G, Lewis B, Glader B, Katz J, Dietrich S, et al; Childrens Hospital of Los Angeles, Los Angeles, California, USA; Comprehensive Care is provided to 90% of hemophiliacs in California by 13 centers. For the year 1987, these centers reported on patient HIV antibody status, age of patient, level of clotting factor deficiency and hemophilia type. The number of AIDS cases in California for the purposes of this study, was that reported t
Prevalence of anti-HIV in i.v. drug users of Latina Province, Italy.: Int Conf AIDS 1989 Jun 4-9; 5:171 (abstract no. A.566); Perroni L, LeFoche L, Mercurio V, Tacconi L, Zanini R, Soscia F; Dept. of Infectious Diseases, Latina General Hospital, Latina. Italy; To investigate the prevalence of HIV infection in i.v. drug users (i.v.d.u.) and to assess the distribution of other known risk factors. All the i.v.d.u. attending public methadone maintenance clinics in the province of Latina, were offered the HIV antibody test. Subjects repeatedly HIV positive (El
36 months follow-up of HIV-1 seropositive individuals.: Int Conf AIDS 1989 Jun 4-9; 5:172 (abstract no. A.567); Mezzaroma I, Papetti C, Pesce AM, D'Offizi GD, Pinter E, Luzi G, Aiuti F; Dept. of Allergy and Clinical Immunology, University of Rome "La Sapienza", Rome, Italy; - To evaluate the re-classification risk in HIV-1 seropositive patients at various clinical stages according to the CDC system. - Patients belonging to at risk categories were included: 25 homosexuals, 211 drug addicts, 22 heterosexuals. They were classified at the first medical check according to t
Heterosexual transmission of HIV infection in a group of population of Madrid City.: Int Conf AIDS 1989 Jun 4-9; 5:172 (abstract no. A.569); Ortiz C, Babin F, Andreu P, Bru F, Pascual I, Colomo C; C.P.S. Centro, Madrid Council, Madrid, Spain; Epidemiological study of HIV infection in 318 subjects with the only risk of heterosexual transmission. Computerized data of Internal Medicine and Dermatology Units of the Clinic were used for the analysis. Twenty two social and sanitary parameters of 318 patients (51 men and 267 women) were followe
Safe and unsafe sexual practices among homosexual men, and the risk of HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:172 (abstract no. A.570); Messiah A, Bucquet D, Rouzioux C, Mettetal JF; INSERM, Hopital Necker, Paris, France; To assess the risk of HIV infection among homosexual men, with regard to safe and unsafe sexual practices. Cross-sectional study among 300 homosexual outpatients of general practitioners and venerologists of the Paris area. The subjects were of unknown or of previously negative serological status. B
[IM-9 classification for HIV infections in the french experience of hospital morbidity]: Int Conf AIDS 1989 Jun 4-9; 5:172 (abstract no. A.571); Boulay F, Pibarot ML, Jestin C, Laporte A, Brunet JB; Direction des Hopitaux, Paris, France
Evaluation of a pediatric AIDS outpatient program.: Int Conf AIDS 1989 Jun 4-9; 5:172 (abstract no. A.572); Perez-Alvarez T, Merodio C, Babin F, Andreu P, Quintero Y, Vivar P; C.P.S. Centro. Madrid Council. Spain; To evaluate the children s AIDS program carried out in an outpatient clinic located in the Centro district of Madrid city. 130 children ranging from the age of 15 days to 13 years were studied. Biological and psychosocial parameters were analiyed. 108 children (83%) were seronegative and 22
First case of HIV1/HIV2 infection in Cameroon.: Int Conf AIDS 1989 Jun 4-9; 5:173 (abstract no. A.573); Kaptue L, Zekeng L, Ngu K, Ouattara A, Monny-Lobe M, Salla R; National AIDS Committee Yaounde, Cameroon; To report the first case of HIV1 and HIV2 infection diagnosed in a Cameroonian patient. Up till June 1988, 6 000 sera were screened for HIV2 antibodies and all were found negative. Systematic screening of patients referred to the University Teaching Hospital, using Elisa test (Behring HIV1 and Elavi
HIV spread in prostitute population in Toulouse (France).: Int Conf AIDS 1989 Jun 4-9; 5:173 (abstract no. A.574); Gayet-Mengelle C, Puel J, Averous S, Bazex J; Laboratoire de Virologie, CHU Purpan, Toulouse, France; To assess the risk of contamination through prostitution (male or female) in our city. We systematically screened anti HIV-1 antibodies in 97 female prostitutes (9 coming from Ghana ) and 16 transvestites or transsexuals, regularly consulting at the Venereal Diseases Centre since May 1985, to Januar
The new case definition criteria, its impact on epidemiological surveillance in Spain.: Int Conf AIDS 1989 Jun 4-9; 5:173 (abstract no. A.575); De Andres R, Tello O, Medrano MJ; Secretaria General del Plan Nacional sobre el SIDA, Madrid, Spain; Cumulative AIDS cases in Spain on date December 21st. 1988 are 2165. Modifications of case definition criteria for reporting AIDS were undertaken in August 1987 by CDC, accepted by WHO in September and applied by the European countries since January 1st 1988. We have studied the impact of these new criteria of case def
Prevalence of different retrovirus in Spanish intravenous drug users during 1.987-1.988. Clinical findings of seropositives.: Int Conf AIDS 1989 Jun 4-9; 5:173 (abstract no. A.576); Contreras G, Garcia Saiz A, Vallhonrat E, Varela JM, Peres Alvarez L, Najera R; Instituto de Salud Carlos III, Madrid, Spain; - To analyze the seroprevalence of HIV-2 and HTLV-I in Spanish intravenous drug users (IVDA) and the clinical findings of seropositives to HIV-1 in relationships to the antigenemia status. We determine as well -- the incidence of coinfection with other infectious agents. - Sera collected from 4.269
Control of HIV transmission in the national blood bank in Mali.: Int Conf AIDS 1989 Jun 4-9; 5:173 (abstract no. A.577); Maiga MK, Traore S, Guindo A, Fofana O; Comite de lutte scientifique et technique contre le SIDA, Bamako, Mali; Since the beginning of the national AIDS program in Mali in 1987, the national blood bank was one of the strategic points for detection, prevention of the transmission of the human retrovirus and the follow up of the prevalence of HIV infection among blood donors. Blood donation was performed from pregnant women, young
The new case definition criteria, its impact on epidemiological surveillance in Spain.: Int Conf AIDS 1989 Jun 4-9; 5:173 (abstract no. A.578); De Andres, Tello O, Medrano MJ; Secretaria General del Plan Nacional sobre el SIDA, Madrid, Spain; Cumulative AIDS cases in Spain on date December 21st. 1988 are 2165. Modifications of case definition criteria for reporting AIDS were undertaken in August 1987 by CDC, accepted by WHO in September and applied by the European countries since January 1st 1988. We have studied the impact of these new criteria of case def
AIDS in the Nordic countries - prevention and control.: Int Conf AIDS 1989 Jun 4-9; 5:174 (abstract no. A.579); Westphal-Christensen B, Bjorner J, Eriksson B, Krasnik A; Danish Institute for Health and Nursing Research, Copenhagen, Denmark; Psychological, social, administrative and political aspects of AIDS in the Nordic countries have been reviewed by the Nordic School of Public Health. As expected, the countries appear quite similar. Specified AIDS-commissions have been established, closely linked to governments. Specific target groups like homosexuals
Risk profile for HIV infection at a sexually transmitted disease clinic.: Int Conf AIDS 1989 Jun 4-9; 5:174 (abstract no. A.580); Beidas S, LoConte J, Byrnes D; Saint Michael's Medical Center, Newark, N.J. USA; To determine HIV risk profile of HIV infected individuals in an STD clinic population, and coexisting STD s at time of diagnosis. METHOD: One hundred and forty patients were counselled and tested for HIV antibody, at an inner city STD clinic over a 7 month period. Thirteen (9 %) patients tested positive for
Evaluation of a multidisciplinary HIV diagnosis and management center, Sydney, Australia.: Int Conf AIDS 1989 Jun 4-9; 5:174 (abstract no. A.581); Morlet A, Michelmore H, Turbitt P, Begley K, Gold J; Albion Street (AIDS) Centre, Sydney Hospital, Sydney, Australia; To provide a multidisciplinary diagnosis, ambulatory care and early intervention service for persons infected with HIV. METHOD: The Albion Street (AIDS) Centre, established in March 1985, is staffed by 45 full time professional and support staff including doctors, nurses, counsellors and research officers. T
Viral hepatitis B(HBV) and AIDS in risk communities in the North-East of Argentina (NEA).: Int Conf AIDS 1989 Jun 4-9; 5:174 (abstract no. A.582); Fay O, Biglione J, Fernandez E, Galindez J, Guagnino L, Navarret N; Centro de Technologia en Salud Publica, UNR, Argentina; To study the attack rate of HBV and HIV in drug addicts (IVDA) and homosexuals (HS), compared with the general population as per samples collected from voluntary blood donors (VBD). Studies were made on samples from communities seeking medical advice in response to COPRES appeal. HBV-carriers were d
Lymphadenopathy is a weak predictor of clinical outcome in HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:174 (abstract no. A.583); Lang W, Anderson R, Royce R, Shiboski S; Children's Hospital, San Francisco, CA, USA; To determine the relationship of lymphadenopathy to clinical outcome in HIV infection. Lymphadenopathy was measured by trained examiners in a population-based sample of 385 men from the San Francisco Men s Health Study who were seropositive at entry in Fall 1984. The incidence of symptoms of progres
Diagnosis and mortality trends in an outpatient AIDS clinic.: Int Conf AIDS 1989 Jun 4-9; 5:174 (abstract no. A.584); Keyes C, Kelly J, Marte C; Bellevue Hospital Center/Community Health Project NY, NY USA; To review trends in diseases of diagnosis and death in a community based primary care AIDS clinic population as a basis for improved management. All cases (169) of AIDS diagnosed from 1/1/86 to 1/1/89 in a primary care out-patient AIDS clinic were reviewed for index diagnosis, year of diagnosis, dia
Serological trend for 22 Mexican homosexual patients that are symptomatic and asymptomatic seropositive.: Int Conf AIDS 1989 Jun 4-9; 5:175 (abstract no. A.585); Morales C, Romero M, Delgadillo S, Dominguez J; Centro Nacional de la Transfusion Sanguinea, Mexico, D.F., Mexico; To determine the presence of HIV Ag and the absence of p24 for one full year in 22 patients that were positive asymptomatic and symptomatic. The study was done in 16 asymptomatic patients, 3 in phase I, 2 in - phase II, and 1 with ARC. A determination of ENV/CORE and detection of HIV Ag in serum was
Prevalence of HBV and HDV markers in patients infected with HIV in Buenos Aires.: Int Conf AIDS 1989 Jun 4-9; 5:175 (abstract no. A.586); Fainboim H, Benetucci J, Astarloa L, Gomez N, Castello T, Schulkin S, Compagnucci A, da Bouza J, Corti M, Diaz Lestrem M; Hospital F.J. Muniz. Buenos Aires. Argentina; The purpose of this study is to evaluate the prevalence of HBV and HDV markers in a group of patients infected with HIV. 96 patients (70 males-26 females between 15 and 53 years) were studied. 72 were IVDA, 17 homosexual, 6 partners of HIV(+) patients and 1 hemophilic. The HIV seropositivity was det
[High prevalence of HTLV-I and low prevalence of HIV in ivory coast lepers]: Int Conf AIDS 1989 Jun 4-9; 5:175 (abstract no. A.587); Sangare A, Verdier M, Sorro B, Gershy-Damet GM, Leonard G, Francois D; Institut Pasteur et, Cote d'Ivoire
Epidemiologic surveillance of human immunodeficiency virus in the industrial city of Siberia.: Int Conf AIDS 1989 Jun 4-9; 5:175 (abstract no. A.588); Denisenko BA, Bulwachter LA, Malphanov AD; Head, Chair of Therapy, Institute for Advanced Medical Studies, Novokuznetsk, USSR; Investigation of the epidemiology of human immunodeficiency virus (HIV) is based on case detection in the Centers for Disease Control. As of December 1988, 45,807 cases of HIV have been tested in non-endemic areas. In the first stage of our investigation the inquiry-test data for all persons with clinically manifested
AIDS: knowledge and attitudes among different ethnic groups.: Int Conf AIDS 1989 Jun 4-9; 5:175 (abstract no. A.589); Eskander GS, Jahan M, Carter R; Meharry Medical College, Nashville, Tennessee, USA; AIDS is an international as well as a national public health problem. Since the identification of acquired immunodeficiency syndrome (AIDS) in 1981, the number of cases has steadily increased throughout the world. The World Health Organization estimated that the world total of AIDS cases will reach up to three million
AIDS on Long Island.: Int Conf AIDS 1989 Jun 4-9; 5:175 (abstract no. A.590); Agins BD, Montgomery N, Forlenza S; Nassau County Medical Center, East Meadow, New York; To describe the characteristics of the AIDS epidemic on suburban Long Island. A retrospective review of epidemiologic and demographic data from the records of AIDS patients seen at Nassau County Medical Center (NCMC) during 1987 and 1988 was performed. Nassau County is the suburban area wit
Electronic reporting system for AIDS surveillance in Canada.: Int Conf AIDS 1989 Jun 4-9; 5:176 (abstract no. A.591); Nault P, Elmslie K; Federal Centre for AIDS, Health Protection Branch, Ottawa, Ontario, Canada; To describe the development of an electronic system for national reporting of AIDS surveillance data. AIDS is a reportable condition in all provinces and territories in Canada . Through collaborative efforts between the provincial/territorial Ministries of Health and National Health and Welfare, a s
An update on HIV-seroprevalence studies in the Netherlands.: Int Conf AIDS 1989 Jun 4-9; 5:176 (abstract no. A.593); Lumey LH, Houweling H, Jager JC; Natl Inst Publ Hlth Env Hyg (RIVM), Bilthoven, the Netherlands; Currently available data on HIV seroprevalence among selected groups in the Netherlands are inadequate to assess the spread of the epidemic among homosexual men, intravenous drug users or the population-at-large. So far, only volunteer studies have been conducted. Additional surveys are required: 1) to establish a base
[The demand for HIV serology examination in general medicine]: Int Conf AIDS 1989 Jun 4-9; 5:176 (abstract no. A.595); Van Casteren V, Stroobant A; Institut D'hygiene ET D'epidemiologie, Bruxelles, Belgique
Prevalence of HIV-1 antibody among gay men in the UK: differences between clinic attenders and non-attenders.: Int Conf AIDS 1989 Jun 4-9; 5:176 (abstract no. A.597); Davies P, Coxon AP, McManus TJ, Sutherland S; Project SIGMA, South Bank Polytechnic, London, UK; To estimate the prevalence of HIV-1 antibody in the homosexually active male population of England and Wales and, bearing in mind that current estimates of prevalence and possible spread are based on those attending STD clinics, to estimate the differences between clinic attenders and non-attenders.
Outreach to IV drug users and sex partners: satisfactions and dissatisfactions of outreach workers.: Int Conf AIDS 1989 Jun 4-9; 5:177 (abstract no. A.598); Stull C, Deren S, Graves F, Sufian M, Friedman SR, Tross S; Narcotic and Drug Research Inc., New York, New York, USA; Ex-addict workers are widely used for AIDS outreach to IVDUs and their sex partners. Our objective is to describe the characteristics of indigenous ex-addict outreach workers hired to provide AIDS prevention and education information to intravenous drug users and their sex partners in New York City, and to d
Decontamination of an HIV contaminated CPR manikin.: Int Conf AIDS 1989 Jun 4-9; 5:177 (abstract no. A.599); Corless I, Lisker A, Buckheit RW, Smiley ML, Langlois AJ, Weinhold K; University of North Carolina-Chapel Hill, North Carolina; Manikins are used widely for cardiopulmonary resuscitation (CPR) training. Simulating a worst-case scenario , a manikin was contaminated in different experiments with either cell-free or cell associated HIV-1. Recommended decontamination procedures were compromised by maintaining a wet surface for 10 or 5 se
Radioresistance of HIV-1 in a bone allograft model.: Int Conf AIDS 1989 Jun 4-9; 5:177 (abstract no. A.600); Conway B, Tomford WW, Hirsch MS, Schooley RT; Infectious Diseases Unit, Massachusetts General Hospital Boston, MA, USA; To study the feasibility of inactivating HIV-1 in bone graft material by gamma irradiation. Serial dilutions of HIV-1 (10(1)-10(6) TCID50/ml) were subjected to gamma irradiation under conditions simulating those within a bone allograft. Radiated and control specimens were cultured on H9 cells which
Resistance of human immunodeficiency virus to disinfectants and fixatives.: Int Conf AIDS 1989 Jun 4-9; 5:177 (abstract no. A.601); Hanson P, Gor D, Jeffries DJ, Collins JV; Brompton and St. Stephen's Hospitals, London; Previous reports that HIV is susceptible to disinfectants are based on suspension tests. We tested 70% alcohol (IMS, Alcohols Ltd.; final concentration ethanol 63.8%, methanol 3.5%, water 26.5% v/v), acetone, 1% and 2% alkaline glutaraldehyde (Cidex, Surgikos Ltd) against HIV dried on a surface. The following inocula w
Potential for HIV transmission via blood glucose monitoring devices.: Int Conf AIDS 1989 Jun 4-9; 5:177 (abstract no. A.602); Shalit P, Brennan C, Murphy V, Hooton T; University of Washington, Harborview Medical Center, Seattle, Washington, USA; We are following a 43 year old HIV-positive insulin-dependent diabetic woman whose only known exposure to HIV was from vaginal intercourse with her HIV-positive bisexual husband. The woman indicated to us that since 1981 she has screened family members, friends, and relatives for diabetes by performing fingerstick gluc
Similar magnitude of the AIDS epidemics among homosexual men in Costa Rica and in the United States.: Int Conf AIDS 1989 Jun 4-9; 5:178 (abstract no. A.603); Mata L, Valadez J; INISA, University of Costa Rica, San Pedro, Costa Rica; To compare the incidence of AIDS in homosexual and bisexual men in Costa Rica , with that in the United States , from the year the epidemics were first recognized ( year one ). Year one was 1981 for the U.S.A. and 1985 for Costa Rica. Cumulative incidence rates (per million) were calculated using th
Heterosexual contact patterns of 429 pregnant non-IV drug using women in the South Bronx, NY, in 1988.: Int Conf AIDS 1989 Jun 4-9; 5:178 (abstract no. A.604); Drucker E, Schoenbaum EE, Webber MP, Stern LS, Vermund SH, Gayle H; Montefiore Medical Center, Bronx, NY, USA; To quantify the probability of heterosexual exposure to HIV-1 for non-IV drug using sexually active women in an area with high levels of HIV-1 (especially among males age 25-34); and to explain known differences in seroprevalence by age, geography, and race/ethnicity among women of childbearing age. METHOD:
Multiple group models with heterogeneous mixing for the transmission dynamics of HIV/AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:178 (abstract no. A.605); Castillo-Chavez C, Cooke K, Huang W, Levin SA; Cornell University, Ithaca, NY, US; Specify ways of constructing multiple group models that take into consideration the social/sexual structure of a population; to present analytical results for single and multiple group models for the spread of HIV/AIDS; and to discuss the consequences of the nonstandard behavior observed in the multiple grou
Perinatal and heterosexual transmission through HIV-positive women.: Int Conf AIDS 1989 Jun 4-9; 5:178 (abstract no. A.606); Rehmet S, Staszewski S, Wegerich B, Kreuz W, Stille W; Zentrum der Inneren Medizin, Zentrum der Kinderheilkunde, Universitatsklinik Frankfurt, FRG; Since 1984, we have examined HIV-infected women and their partners in order to gain information on infectivity, perinatal & heterosexual transmission rates, and possible relations between these factors. 44 Women examined in this period had born a total of 47 children since their HIV infection. 10 children are posit
Additional risk factors in HIV positive persons as monitored by a national HIV notification system.: Int Conf AIDS 1989 Jun 4-9; 5:178 (abstract no. A.607); Hasseltvedt V, Lystad A, Nilsen O; National Institute of Public Health, Oslo, Norway; To study additional risk factors (e.g. sexual contact with person known to be HIV positive, sexual contact in a hyperendemic country, sexual contact with prostitutes), regarding persons notified as HIV positive where transmission category is known. METHOD: The Department of Infectious Disease Control (DIDC),
Comparison of survival experiences of New York and California Medicaid AIDS patients.: Int Conf AIDS 1989 Jun 4-9; 5:178 (abstract no. A.608); Markson L, Keyes M, Turner B; Jefferson Medical College, Philadelphia, PA; To compare the survival experiences, hospital length of stay and costs of care of Medicaid AIDS patients in New York and California adjusting for severity level at the first recorded AIDS hospitalization. Medicaid claims for AIDS patients in New York and California from 1983-1987 are linked in order
Predicting in-hospital mortality for AIDS patients: a validated model.: Int Conf AIDS 1989 Jun 4-9; 5:179 (abstract no. A.609); Grover SA, Gilmore N, Falutz J, Tsoukas C, Coupal L, Fakhry R; Montreal General Hospital, McGill University, Montreal, PQ; Estimating prognosis for a hospitalized AIDS patient is essential for optimal clinical management. We developed a prediction model for in-hospital mortality using admission data abstracted from 128 patient admissions to the Royal Victoria Hospital (RVH). This model was subsequently validated on 62 patient admissions to
Evolution of the clinical and laboratory parameters of one patient with HIV infection in the first year after his documented date of seroconversion.: Int Conf AIDS 1989 Jun 4-9; 5:179 (abstract no. A.612); Georgopoulou SA, Ploumidis GM, Roumeliotou AN, Papadopoulos AI, Stergiou GD; 1st IKA Hospital, Athens, Greece; To present the pattern of changes in the clinical and laboratory findings observed during the first year after the date of seroconversion. METHOD: A HIV-seronegative patient was admitted to our hospital suffering from a mononucleosis - like syndrome. On the 23rd day of his hospitalization and while he was fr
Influence of HIV on prison inmate (PI) dialysis (D) trends.: Int Conf AIDS 1989 Jun 4-9; 5:179 (abstract no. A.613); Glaser J, Kaplan J, Laio P, Kleiner M, Sudberg L, McGinn J; Richmond Memorial & Staten Island Hospitals, SI, NY, USA; PURPOSE: To assess the influence of HIV on PI D trends at AKCF. Hospital and D unit record review. From 7/82 thru 12/88, 64 male NY State PIs underwent D, including 50 IVDUs and 14 non-IVDUs. Early (7/82-12/84) and later (1/85-12/88) D experiences for IVDUs were compared. No difference in: % of pts. w
HIV infection in patients attending a hospital based sexually transmitted diseases clinic.: Int Conf AIDS 1989 Jun 4-9; 5:179 (abstract no. A.614); DeHovitz J, Freedman P, Bastien A, Norat Y; AIDS Prevention Center, SUNY - HSC @ Brooklyn, USA; To determine HIV seroprevalence in patients who are attending STD clinic in a high prevalence area. All patients attending an urban hospital-based STD clinic were offered HIV-1 testing in a period between 8/88 and 12/88. Patients were given pretest, with posttest counseling scheduled two weeks follo
Risks associated with acquiring chancroid genital ulcerative disease and HIV infection: a case control study.: Int Conf AIDS 1989 Jun 4-9; 5:180 (abstract no. A.615); McLaughlin M, Wilkes M, Blum S, Hermoso A, Schmid G; New York City Department of Health, NY, NY, USA; To identify risks associated with acquiring chancroid and to evaluate the seroprevalence of human immunodeficiency virus (HIV) infection among men presenting with chancroid and gonorrhea to a New York City Department of Health sexually transmitted diseases clinic. This case-control study was perform
Loss of serologic and virologic markers of HIV-1 in asymptomatic HIV-1 infected homosexual men.: Int Conf AIDS 1989 Jun 4-9; 5:180 (abstract no. A.616); Farzadegan H, Wolinsky S, Sninsky J, Kwok S, Rinaldo C, Phair J, Kaslow R, Saah A; Multicenter AIDS Cohort Study (MACS), Bethesda, MD, USA; To follow virological and serological changes in 4 asymptomatic homosexual men who seroreverted from positive to negative for HIV-1 during the initial 2-2.5 yrs of evaluation. Peripheral blood mononuclear cells (PBMCs) of these participants showed detectable HIV-1 DNA by the polymerase chain reaction (PCR) m
HIV infection in lorry drivers and their assistants in eastern Africa.: Int Conf AIDS 1989 Jun 4-9; 5:180 (abstract no. A.617); Carswell JW, Namaara W, Lloyd G, Howells J; Family Health International, Research Triangle Park, North Carolina, USA; To quantify prevalence of HIV infection among long distance lorry drivers, and their assistants, in eastern Africa. Sixty-eight lorry drivers and their assistants were tested for HIV while in Kampala, Uganda , in October 1986. Data were collected on travel history, prostitute contact and history of
Risk of infection by human immunodeficiency virus in health care workers (HCW).: Int Conf AIDS 1989 Jun 4-9; 5:180 (abstract no. A.618); Hernandez E, Gatell JM, Puyuelo T, Barrera JM, Pumarola T; Occupational Health Service. Infec. Diseases Unit. Haemostasy Unit. Hospital Clinic. Barcelona. Spain.; To evaluate the risk of occupational transmission of HIV. We have prospectively followed a cohort of 76 HCW who reported at least one episode of direct exposure to body fluids of HIV infected patients (presence of antibodies against the HIV by at least two ELISA test and confirmed by Western Blot in
AIDS surveillance in Sichuan, China.: Int Conf AIDS 1989 Jun 4-9; 5:180 (abstract no. A.619); Dongyou Y, Chen L, Lu B, Deng P; Health and Anti-Epidemic Centre of Sichuan Province, Chengdu, Sichuan Province 610031, People's Republic of China; To monitor introduction of AIDS from the outside world. We used antigen slides of T-cell line infected with HIV and indirect immunofluorescent antibody technique (IFA) for the detection of antibodies against HIV in sera of persons at high risk. So far, in our laboratory 1,327 sera collected
The presence of tumor necrosis factor (TNF) in serum and in lymphocyte cultures of HIV-positive and negative homosexual subjects.: Int Conf AIDS 1989 Jun 4-9; 5:180 (abstract no. A.620); Buimovici-Klein E, Lange M, Mohan D, Sonnabend JA, Grieco MH, Cooper LZ; St. Luke's-Roosevelt Hospital Center, Columbia University, New York, New York 10025; Recent reports have described the presence of TNF in the sera of HIV-positive symptomatic (ARC, AIDS) individuals. We, therefore, attempted to correlate the presence of TNF in serum and in the supernatants of lymphocyte cultures with the presence of various viruses isolated from different body sites in HIV p
The search for a cofactor in AIDS-related Kaposi's sarcoma (KS): assessment of nitrite inhalant use in studies of homosexual men with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:181 (abstract no. A.621); Haverkos H, Drotman DP; National Institute on Drug Abuse, Rockville, Maryland, USA; The epidemiology of KS in AIDS (the concentration among white homosexual men and the declining proportion of cases) suggests that HIV may not act alone as its cause. In transfusion-acquired AIDS from donors in whom KS subsequently developed, KS is rare, suggesting that a specific strain of HIV is not responsible. The u
Occurrence and prognostic value of AIDS-related clinical manifestations in HIV-1 seropositive homosexual men.: Int Conf AIDS 1989 Jun 4-9; 5:181 (abstract no. A.622); Kaslow RA, Blackwelder W, Visscher B, Friedman H, Anderson R, Fox R, MacDonell K, Kuo V; Multicenter AIDS Cohort Study (MACS). Bethesda, MD, USA; To use a clinical index to quantify early clinical progression of HIV-1 infection and determine the prognostic value of a single examination. A clinical index derived from early symptoms and signs of HIV-1 infection was used to score men based on data collected semiannually. Proportions of men with
Detection and quantification of HIV-1 antibodies in semen.: Int Conf AIDS 1989 Jun 4-9; 5:181 (abstract no. A.623); Wolff H, Martinez A, Politch J, Saltzman S, Mayer K, Anderson DJ; Harvard Medical School, Boston, MA; To measure levels of HIV-1 antibodies (Abs) of IgG, IgA and IgM subclasses in human seminal plasma (SP) from HIV seropositive men, and to compare titers to those in matched serum. Semen was obtained from 28 HIV-1 seropositive men and from 16 seronegative men. Corresponding serum samples were obtaine
Electron microscopic (EM) analysis of semen from HIV seropositive males.: Int Conf AIDS 1989 Jun 4-9; 5:181 (abstract no. A.624); Pudney J, Wolff H, Anderson D; Harvard Medical School, Boston, MA, USA; To determine if the structural composition of semen changes following infection with HIV. Transmission EM analysis of semen samples from HIV+ males (n=20). Normal semen contains spermatozoa plus a variable population of round cells composed of white blood cells (WBC) and immature germ cells
Quantification of CD4 lymphocytes, macrophages and HIV antigen levels in vaginal secretions of HIV+ women.: Int Conf AIDS 1989 Jun 4-9; 5:181 (abstract no. A.625); Hill JA, Carpenter CC, Martinez A, Politch JA, Mayer K, Anderson DJ; Harvard Medical School, Boston, MA; To quantitate HIV host cells (HHC) and HIV antigen in vaginal secretions (VS) of HIV seronegative women (n=5) and asymptomatic HIV+ women (n=7), women with CDC IV non AIDS (n=5), and women with AIDS (n=6). 30 VS were obtained from 18 HIV+ women and 20 VS from 5 low risk HIV- women. Cell smears were
Pets and HIV disease; a survey of providers' knowledge and attitudes.: Int Conf AIDS 1989 Jun 4-9; 5:181 (abstract no. A.626); Gorczyca K, Abrams D, Carmack B; Pets Are Wonderful Support, San Francisco, California, USA; To assess the knowledge base of physicians expert in the care of individuals with HIV infection with regard to their understanding of zoonotic diseases and their attitudes regarding the interactions of their patients and their pets. A 14 question survey was distributed to membership attending the S
A community-based HIV assessment unit: how does it work?: Int Conf AIDS 1989 Jun 4-9; 5:182 (abstract no. A.627); Morgan A, Davis I, Israni P, Ramis CM, Norris B, Barnwell M; Woodhull Medical & Mental Health Center, Brooklyn, NY, USA; To describe how patients at high-risk for HIV infection are evaluated and treated by an interdisciplinary team of providers at a community-based HIV Assessment Unit. METHODS & Woodhull Medical & Mental Health Center, a division of NYC Health and Hospitals Corporation, is a large municipal ho
Evaluation of compliance rate in a clinic serving minority and low income communities.: Int Conf AIDS 1989 Jun 4-9; 5:182 (abstract no. A.628); Davis I, Leibel J, Paroski P, Laqueur P; Woodhull Medical and Mental Health Center, Brooklyn, NY, USA; To determine compliance rates for HIV-seropositive persons in a socioculturally sensitive clinic (SSC) setting from communities that are considered traditionally noncompliant . METHOD: Missed appointments rates were compared between the SSC and the ambulatory care network (ACN). The overall broken appointmen
Prevalence of antibodies against HIV-1 and HTLV-I in African sera samples collected during the 1960's.: Int Conf AIDS 1989 Jun 4-9; 5:182 (abstract no. A.629); Frenkl T, McGhee B, Lewinski C, Longiaru M, Nair RN, Pottathil R; Roche Diagnostic Systems, Inc., Nutley, New Jersey, USA; To study the prevalence of HIV-1 and HTLV-I infections during the 1960 s in Ghana among the Akan, Ga-Adangbe, and Ewe tribes. 404 archived sera samples from Ghana collected as early as 1960 were used in this study. Sera was screened for the presence of antibodies against HIV-1 and HTLV-I using Roche
Training the health care professional: the AIDS Regional Education and Training Centers Program of the Health Resources and Services Administration (HRSA).: Int Conf AIDS 1989 Jun 4-9; 5:182 (abstract no. A.630); Horner J, Heffron P, Martell B, Moore D, Macher A; Division of Medicine, Bureau of Health Professions, HRSA, United States Public Health Service, Rockville, Maryland, USA; To establish regional AIDS education centers that will provide multidisciplinary training for health care professionals. With a projected cumulative total of more than 300,000 AIDS cases by 1992, it is essential that health care providers be trained in the diagnosis, management and treatment of HIV-
Increased susceptibility of unstimulated peripheral blood mononuclear cells (PBMC) and CD8+ cell-depleted PBMC to in vitro infection with HIV after ingestion of 2 to 4 beers.: Int Conf AIDS 1989 Jun 4-9; 5:182 (abstract no. A.631); Bagasra O, Lischner HW, Kajdacsy-Balla A; Univ. of Med. & Dent. of New Jersey, Camden, NJ, USA; To determine the duration of the previously described effects of acute ethanol ingestion on HIV infection of PBMC in vitro (FASEB J 2:A831, 1988) and the influence of removal of CD8+ cells on these phenomena. PBMC isolated from 3 healthy non-alcoholic volunteers before and almost daily after consump
The opportunists in human AIDS (HAIDS) are zoonotic.: Int Conf AIDS 1989 Jun 4-9; 5:182 (abstract no. A.632); Torres-Anjel MJ, Rueda RH; Univ. of MO Columbia, USA; To explore animal diseases and animal products with respect to HAIDS. MATERIALS: The opportunists associated to HAIDS were searched in the AIDSLINE database with GRATEFUL MED (TM) software and scrutinized vis-a-vis the concept of zoonoses (1). The number of publications devoted to each were recorded (in pare
Epidemiologic study of children of HIV infected women in primary health care.: Int Conf AIDS 1989 Jun 4-9; 5:183 (abstract no. A.633); Perez-Alvarez T, Merodio C, Babin F, Ortiz C, Colomo C, Sastre J; C.P.S. Centro. Madrid Council. Spain; To show social and sanitary variables influencing HIV positive children and HIV negative children, of HIV infected women before pregnancy. Analytic and descriptive study of 40 social and sanitary parameters obtained of cases studied in the pediatric unit between January 87 and January 89. C
HIV-2 infection in Sardinia.: Int Conf AIDS 1989 Jun 4-9; 5:183 (abstract no. A.634); Pintus A, Piras S, Contu L, Pitzus F; Istituto di Clinica Medica, Universita di Cagliari, Italy; HIV-2 infection has been found only in few cases in Italy but no data are, by now, known about the presence or absence of this virus in Sardinia where there are about 15.000 drug abusers (DA) on 1.500.000 inhabitants and 1500 beta-thalassemic (BT) patients. Considering that AIDS cases are rapidly increasing
[Heterosexual transmission of HIV: hospital surveillance in Aquintaine, France - 1985-88]: Int Conf AIDS 1989 Jun 4-9; 5:183 (abstract no. A.635); Poumerol G, Chaslerie A, Morlat P, Lacoste D, Dupon M; Department d'Informatique Medicale, Universite de Bordeaux II, Bordeaux, France
[Epidemiological characteristics of new HIV seropositives in central-western France: evaluation of incidence and behavior]: Int Conf AIDS 1989 Jun 4-9; 5:183 (abstract no. A.636); Marchand S, Choutet P; CHRU Bretonneau, 37044 Tours France
Profile of HIV-infected patients at San Francisco General Hospital outpatient clinics.: Int Conf AIDS 1989 Jun 4-9; 5:183 (abstract no. A.637); Wong RJ, Gumbley-Smith D, Volberding P; Department of Medicine, San Francisco General Hospital, San Francisco, California USA.; To document characteristics of patients who receive medical care at SFGH AIDS adult outpatient clinics. Of 2000 patients receiving primary care at SFGH AIDS clinics, 100 charts were randomly selected from a computer list of visits from December 8 to 13, 1988. There were 92 males and 8 femal
A community-based model for primary care at an HIV antibody alternate test site.: Int Conf AIDS 1989 Jun 4-9; 5:183 (abstract no. A.638); Howard J, Mahon R, Duynstee S, Walker M, Brooks S; University of California, Irvine, CA, USA; We set up a community-based clinic in Los Angeles at a HIV antibody alternate test site for the medical and psychological evaluation of antibody-positive testees. Health history, physical exam, laboratory tests and psychological assessment were obtained on 1200 subjects from the alternate test site
Health workers for the AIDS epidemic in the USA: what we know and what we need to know.: Int Conf AIDS 1989 Jun 4-9; 5:184 (abstract no. A.639); Hall T, Dunham N, Wittenberg E, Knickman J; Univ. of Calif./San Francisco, USA; To define available and needed information regarding health worker training and deployment to cope with AIDS. The literature through 1988 was reviewed and 29 persons knowledgeable about different aspects of staff training and utilization were interviewed. More than 100 relevant citations we
Semen banks/embryo banks: potential hazard of HIV transmission: an overview.: Int Conf AIDS 1989 Jun 4-9; 5:481 (abstract no. B.501); Hafez ES; World Federation of Contraceptive Health, Reproductive Health Center, Surfsong Road, Kiawah, SC, USA; Donor artificial insemination (AID) and in vitro fertilization (IVF) using human fresh semen and cryopreserved/thawed semen from HIV-seropositive or asymptomatic donors play a critical role in HIV transmission. HIV and various strains of STD s can adhere to and penetrate the plasma membrane of human sperm head/midpiece
Contraceptives, HIV infections and heterosexual transmission.: Int Conf AIDS 1989 Jun 4-9; 5:481 (abstract no. B.502); Hafez S, El Tonsi H, Lofty M; Reproductive Health Center, Kiawah Island, SC, USA; Contraceptive copper IUD s, contraceptive diaphragms, rubber condoms, and spermicides may produce allergic contact dermatitis in sensitized persons. Rubber chemicals may cause condom dermatitis/diaphragm balanitis in rubber sensitive men, and vulvititis/vaginitis in rubber-sensitive women. Dermatitis may be due to sens
[Prospective study of HIV infection in pregnant women: surveillance of 96 patients, 51 infants and 49 families]: Int Conf AIDS 1989 Jun 4-9; 5:481 (abstract no. B.503); Ciraru-Vigneron N, Nguyen Tan Lung R, Brunner C, Honderlick P, Wautier JL, Ravina JH; Hopital Lariboisiere, Universite Paris VII, Paris, France
HIV infection rates in parturients who receive no prenatal care.: Int Conf AIDS 1989 Jun 4-9; 5:481 (abstract no. B.504); Killian H, Risi G, James D, Muirhead L, Shields J, Marier R; Department of Infection Control, Charity Hospital, New Orleans, LA, USA; To test the hypothesis that women receiving no prenatal care represent a higher risk group for HIV infection than those who receive regular care at an urban hospital serving primarily indigent patients (Charity Hospital [CH]). All women presenting to the obstetrical clinics of CH are offered HIV tes
Massage for terminally ill AIDS patients.: Int Conf AIDS 1989 Jun 4-9; 5:481 (abstract no. B.505); Ruebottom A, Lee C, Dryden PJ; Casey House Hospice Inc., Toronto, Ontario, Canada; To describe the pioneering venture of using Registered Massage Therapists (RMTs) in a healthcare institution and to outline the massage techniques and modalities contributing to the care of terminally ill AIDS patients at Casey House. The activities within the scope of practice of RMTs in Ontario ar
Evaluating the outcomes of a linkage system designed to facilitate access for chronically homeless persons to an outpatient AIDS program.: Int Conf AIDS 1989 Jun 4-9; 5:481 (abstract no. B.506); Hagerty CA, O'Connell JJ; Boston City Hospital, Boston, MA, USA; SIGNIFICANCE: The numbers of known HIV+ residents residing in Boston s shelters has grown from 1, in 1985, to more than 75 in 1988. Chronically homeless constitute 25% of the Aids program patients. Our primary effort has been to broaden AIDS-specific health care to our city s chronically homeless population
Comprehensive out-patient care of HIV-infected patients utilizing a primary care model.: Int Conf AIDS 1989 Jun 4-9; 5:482 (abstract no. B.507); Anastos K, Soloway B, Ernst J; Bronx-Lebanon Hospital Center, Bronx, New York, USA; To meet the total health needs of HIV infected individuals, of all ages and all states of illness, in a community with very high HIV seroprevalence. METHOD: The establishment of a comprehensive out-patient health care program for HIV-infected individuals. A community hospital in the South Bronx has
AIDS and physical education.: Int Conf AIDS 1989 Jun 4-9; 5:482 (abstract no. B.508); Freire SV, Machado IZ; Centro Corsini, (CCII), Campinas, Sao Paulo, Brazil; The human is an integral composition of mind and body represented by pleasure and aesthetics, emotions and reason. The body is the very first human experience moment. The individual senses and lives prior to knowing, participating through the body in real life. However the individual is being threatened by the AIDS vir
Long-term follow-up untreated-severe thrombocytopenia (STP) in three HIV infected drug addicts.: Int Conf AIDS 1989 Jun 4-9; 5:482 (abstract no. B.509); Tural C, Clotet B, Sirera G, Romeu J, Casals A, Tor J, Foz M; Infectious Disease Unit, Hospital de Badalona "Germans Trias i Pujol", Barcelona, Catalonia, Spain; To report the findings and follow-up observed in three seropositive patients who denied any therapeutic approach during a mean period of 18 months (range 27-15). 3 out of 12 HIV seropositive IDAs with severe thrombocytopenia (STP) (platelet count less than 9000/mm(3) and rich marrow aspirate) relate
Fully automated system for the detection and registration of HIV-antibodies in donor blood.: Int Conf AIDS 1989 Jun 4-9; 5:482 (abstract no. B.510); Reesink H, Koot A, Schram N, Plaisir A, Drewes A; Red Cross Blood Bank, Amsterdam, The Netherlands; To develop a fully automated system for the detection and registration of anti-HIV in donor blood. Anti-HIV tests are done with an ELISA system in microtiter plates (Wellcozyme, Wellcome, UK). The automated system functions as follows: microtiter plates are provided with barcoded labels and position
Hemophiliac population of Costa Rica from 1979 to 1989.: Int Conf AIDS 1989 Jun 4-9; 5:482 (abstract no. B.511); Taylor L, Montero C, Cordero R, Astua MC, Roy A, Luftig R, Visona KA; Louisiana State University-International Center for Medical Research & Training, (LSU-ICMRT), San Jose, Costa Rica; To analyze the serological profile in the hemophiliac population in Costa Rica . Serum samples stored in the LSU-ICMRT serum bank were tested with anti-HIV ELISA (Abbott HTLV-III), Western Blot (LSU-ICMRT), HIV antigen (vironostika-Organon), IRMA HBsAg and anti-HBs (LSU-ICMRT). Clinical data were ob
Immune activation in HIV positive and negative hemophiliacs.: Int Conf AIDS 1989 Jun 4-9; 5:482 (abstract no. B.512); Nishida Y, Oura Y, Koyama T, Higuchi M, Kakishita E; Department of Internal Medicine, Hyogo College of Medicine, Hyogo, Japan; To analyze signs of immune activation observed in hemophiliac patients with and without HIV infection. The absolute CD4 number, percent, CD4/CD8 ratio, Ia positive cell percent and Ig (G, A, M) were measured on a cohort of 41 hemophiliac patients (18 seropositive, 23 seronegative) followed for 5 yea
HIV-infection in hemophiliacs in Argentina.: Int Conf AIDS 1989 Jun 4-9; 5:483 (abstract no. B.513); Muchinik G, Perez BR, Bouzas MB, Rickard E, Armando E, de Tezanos Pinto M; IIHEMA, Academia Nacional de Medicina, Buenos Aires, Argentina; To describe an update of HIV-infection in hemophilia. HIV antibodies (ab) were detected through EIA (Abbott) and confirmed by means of IFA and ENVACORE (Abbott). A total of 467 hemophiliacs has been studied since November, 1984. TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSION: Since 1984 and
Findings of indeterminate results to WB (p17, p24, p55) in Mexican normal donors.: Int Conf AIDS 1989 Jun 4-9; 5:483 (abstract no. B.515); Romero M, Morales C, Dominguez J; Centro Nacional de la Transfusion Sanguinea, Mexico, DF; The previous detection (screening) which is done mandatory by law to the blood drawn from normal donors showed the presence of certain bands that made WB results indeterminate such instance took us to search for HIV Ag in order to confirm an early seroconversion or possible cross reaction with another retrov
Platelets in HIV-infection: decrease associated with immunodeficiency, mycobacteriae infection and salmonellae septicemia: pseudothrombocytopenia in HIV-infection.: Int Conf AIDS 1989 Jun 4-9; 5:483 (abstract no. B.516); Matuschke A, Heinrich B, Welzl G, Bogner JR, Fuebetal H, Goebel FD; Univers. Munchen, Med. Poliklinik, FRG; To describe different events associated with platelet reduction in HIV-infection. Classification of immunodeficiency according to Walter Reed Classification System (WR 1-6). Documentation of the first platelet count/mul (PL) at admission, after progression in WR, after developing an opportunistic in
Evaluation of HIV-antibodies in samples of commercial preparations of gamma globulin for parenteral use in the periods 1973-1981 and 1984-1986.: Int Conf AIDS 1989 Jun 4-9; 5:483 (abstract no. B.517); Tinelli M, Cattaneo E, Achilli G; Institute of Infectious Diseases, IRCCS - Policlinico S. Matteo, Pavia, Italy; Evaluation of the presence of HIV-antibodies in gamma globulin different commercial preparations in the periods 1973-1981 and l984-1986. Samples have been screened by ELISA commercial kits and, if positive, confirmed by homemade Western-blot. In period 1973-1981 we tested 13 gamma globulin
Correlation of the HIV and HTLVI antibody, HIV antigen in haematological disease (H.D.).: Int Conf AIDS 1989 Jun 4-9; 5:483 (abstract no. B.518); Granati L, Mandelli F, Isacchi G, De Rossi G, Titti M, Pezzella M; Haematological Section-Human Biopathology Departments Infectious Disease Chair, University of Rome, Instituto Superiore di Sanita, Roma; To investigate the prevalence, the correlation and the follow up of HIV antibody, HIV antigen, HTLVI antibody in H.D., to investigate the significance of indeterminate western blot. During 1988, 1400 patients with H.D. were tested by ELISA (Organon) for HIV antibody (if positive W.B. was performed),
Extrapulmonary cryptococcal disease as a manifestation of AIDS in Louisiana.: Int Conf AIDS 1989 Jun 4-9; 5:484 (abstract no. B.519); Atkinson W, Troxler S, McFarland L; Louisiana Department of Health and Hospitals, New Orleans, LA, USA; To determine the frequency and characteristics of patients with extrapulmonary cryptococcosis (EC) as a manifestation of AIDS in Louisiana (LA) residents between 1981 and December 1988. Cases of AIDS were identified by an active hospital-based surveillance system. Only definitively diagnosed cases m
[Infection by HIV, after a liver transplant: study of 2 patients for 2 years]: Int Conf AIDS 1989 Jun 4-9; 5:484 (abstract no. B.520); Vanhems P, Bresson-Hadni S, Vuitton D, Lab M, Miguet JP; Centre Hospitalier et Universitaire, 25030 Besancon, France
Is entamoeba histolytica in homosexual men a pathogen?: Int Conf AIDS 1989 Jun 4-9; 5:484 (abstract no. B.521); Weinke T, Friedrich - Janicke B, Janitschke K; Universitatsklinikum Rudolf Virchow, Freie Universitat Berlin, West Germany; Entamoeba histolytica occurs frequently among sexually active homosexual men. We tried to evaluate the clinical importance of this protozoa. 320 male homosexuals were screened for intestinal parasites. E. histolytica was cultured in Robinson medium and positive cultures were classified into zymodeme
Spontaneous bacterial peritonitis and AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:484 (abstract no. B.522); Biancalana ML, Mendes WS, Levi GC, Carvalho MC, Mendonca JS, Trevisanello C; Servico de Moletias Infecciosas, Hospital do Servidor Publico Estadual, So Paulo, Brazil; The authors relate a case of a 28-year-old white homosexual man with AIDS, who was being treated for Kaposi s sarcoma and toxoplasmic encephalitis during 7 months when he developed aqueous diarrhea, weakness an diffuse abdominal pain. The physical examination revealed poor general condition, anemia, dehydration, anasar
[Non-lithiasitic cholecystitis, stenosis of the papilla, sclerosing cholangitis and AIDS]: Int Conf AIDS 1989 Jun 4-9; 5:484 (abstract no. B.523); Weinbreck P, Loustaud V, Valleix D, Mounier M, Denis F; Unite des Maladies Infectieuses - Chu Limoges - 87042 France
Spontaneous rupture of spleen in a HIV seropositive non- thrombocytopenic patient.: Int Conf AIDS 1989 Jun 4-9; 5:484 (abstract no. B.524); Pesce A, Vinti H, Taillan B, Fuzibet JG, Dujardin P, Cassuto JP; Services de Medecine Interne I et II - Hopital de Cimiez - CHRU Nice, France; We recently observed a case of rupture of the spleen, in a HIV seropositive asymptomatic and non thrombocytopenic patient in relation with a large spontaneous splenic hematoma. A 25-year- old male patient, intravenous drug abuser from 1982 to 1984, HIV seropositive since February 1986 and regularly followed without any
The prevalence of the hepatitis B virus (HBV) markers in patients who are carriers of anti-HIV in Sao Paulo, Brazil.: Int Conf AIDS 1989 Jun 4-9; 5:485 (abstract no. B.525); Diaz RS, Silva AE, Ferraz ML, Sader HS, Castilho MP, Lewi DS; Escola Paulista de Medicina, Sao Paulo, Brazil; To study the behaviour of VHB infection in patients who are carriers of HIV, separated into two distinct groups evaluated in different times. The two groups were compared between themselves and with a control group of blood donors from a blood bank. MATERIAL AND The patients were divided into two gr
Seroprevalence and clinical correlates of herpes simplex virus (HSV) antibody in 41 AIDS patients.: Int Conf AIDS 1989 Jun 4-9; 5:485 (abstract no. B.526); Safrin S, Houlihan C, Arvin A, Mills J; San Francisco General Hospital, University of California, CA, USA; (1) To assess the clinical course of mucocutaneous HSV in patients with AIDS; (2) To determine the seroprevalence of HSV antibody in this cohort; (3) To analyze the correlation of the presence of serum HSV antibody with clinical herpetic disease activity. Forty-one patients with the diagnosis of fir
Diseases other than AIDS among IV drug users.: Int Conf AIDS 1989 Jun 4-9; 5:485 (abstract no. B.527); Trapido EJ, McCoy CB, Chitwood DD, McCoy HV, Inciardi JA; University of Miami Medical School, Miami, FL, USA; To describe the distribution of diseases other than AIDS among a cohort of IV drug users. As part of a study which tests two interventions to reduce the risk of AIDS among IVDUs, 346 subjects were recruited from drug networks, tested for HIV-1 positivity, and interviewed. Bivariate analyses were per
Increased incidence of M. kansasii infection due to AIDS and HIV infection at Cook County Hospital, Chicago.: Int Conf AIDS 1989 Jun 4-9; 5:485 (abstract no. B.529); Sherer R, Kocka F, Muthuswamy P, Pulvirenti J, Blair S, Sonnenberg M; Department of Medicine, Cook County Hospital, Chicago, IL, USA; To compare the incidence of M. kansasii infection at Cook County Hospital (CCH) from 1977-1982 and 1983-1988, and to evaluate the significance of associated AIDS and HIV infection. All patients with M. kansasii infection between 1977 and 11/88 were cross-referenced with all persons with AIDS and HIV
The Koebner phenomenon in AIDS-related Kaposi's sarcoma.: Int Conf AIDS 1989 Jun 4-9; 5:485 (abstract no. B.530); Janier M, Baccard M, Cabotin PP, Timsit F, Colonna A, Morel P; Centre Clinique et Biologique des MST, Hopital Saint-Louis, Paris, France; The Koebner phenomenon is the development of isomorphic lesions in the traumatized normal skin of patients who have psoriasis, lichen planus or various cutaneous diseases. Kaposi s sarcoma (KS) in its classical form sometimes koebnerizes. We have observed 4 cases of Koebner phenomenon in the course of AIDS-related KS.
Ano-rectal surgery of AIDS patients.: Int Conf AIDS 1989 Jun 4-9; 5:486 (abstract no. B.531); Fraga LF, Bravo FP, Paulo FL, Quinhoes EP, Rachid MC; Gaffree & Guinle University Hospital - University of Rio de Janeiro - Brazil; To discuss the importance of proctologic surgical procedures for HIV infected patients. During 1987 and 1988 we operated 20 HIV infected patients of groups II, III and IV of CDC criteria. All patients were tested for HIV antibodies by ELISA, Indirect Immunofluorescence and/or Western blot.
Radiographic patterns of pulmonary tuberculosis among AIDS patients in Rio de Janeiro, Brazil.: Int Conf AIDS 1989 Jun 4-9; 5:486 (abstract no. B.532); Eyer-Silva W, Graca MH, Camara CS, Teixeira E, Sion FS, Morais de Sa CA; Gaffree & Guinle University Hospital - University of Rio de Janeiro (UNI-RIO) - Brazil; Describe roenotgenographic features of pulmonary tuberculosis among Brazilian AIDS patients. We reviewed standard chest radiographs of 42 patients who met the current CDC criteria for AIDS and who also had a diagnosis of pulmonary tuberculosis. Among 42 patients reviewed, 41 were men and 1
Echocardiographic (ECHO) findings in HIV infected patients.: Int Conf AIDS 1989 Jun 4-9; 5:486 (abstract no. B.533); Talberg J, Morais de Sa CA, Cantisano AL, Oliveira DP, Sion FS, Rubini NPM; Gaffree & Guinle University Hospital, University of Rio de Janeiro, Brazil; To study echocardiographic findings in patients infected by HIV, groups III and IV (C.D.C.), from the out-patients unit and from in-patients of the HUGG, related to the period from August 85 to November 88. Parameters of dimensions, structures movements and cardiac functions were obtained by ECHO in
Cardiologic findings in autopsies of AIDS patients.: Int Conf AIDS 1989 Jun 4-9; 5:486 (abstract no. B.534); Talberg J, Morais de Sa CA, Oliveira CA, Signorine DJ, Graca MH, Ismael C; Gaffree & Guinle University Hospital - University of Rio de Janeiro (UNI-RIO) - Brazil; To study the cardiologic findings in autopsies of AIDS patients performed at the Department of Pathology, Gaffree & Guinle University Hospital. We studied the cardiologic findings in the autopsies of 33 AIDS patients classified individually and per cardiac segment (pericardium, myocardium and en
[Value of synthetic peptides specific for HIV1-HIV2 in blood specimens from Abidjan]: Int Conf AIDS 1989 Jun 4-9; 5:486 (abstract no. B.535); Gueguen M, Abissey S, Ruffault A, Guisth'Au O, Yao T, Genetet B; CRTS, CHU, Abidjan, Cote d'Ivoire
Evaluation of a rapid membrane based assay (HIV CHEK) for detection of antibody to HIV in serum samples from Nairobi.: Int Conf AIDS 1989 Jun 4-9; 5:486 (abstract no. B.536); Moss GB, Maitha G, Plummer FA, Wamola IA, Ndinya-Achola JO, Kreiss JK; Univ. Nairobi, Kenya; Univ. Washington, Seattle, WA, USA; To evaluate a rapid membrane based HIV antibody assay. Serum obtained from various Nairobi clinics where HIV studies are being performed were tested for HIV antibody by ELISA and a rapid membrane based assay (RA) (HIV CHEK, Dupont). Western blots (WB) were performed on samples positive on either ass
Serum LDH isoenzyme activity in HIV patients with respiratory disease.: Int Conf AIDS 1989 Jun 4-9; 5:487 (abstract no. B.537); Young M, McPherson R, Podlasek S, Pierce P; Georgetown University Hospital, Washington, DC, USA; To characterize the serum LDH isoenzyme pattern in HIV patients with elevated LDH and bronchoscopically diagnosed PCP or non-PCP respiratory pathogens. 14 HIV pts presenting with fever, cough, SOB or retenographic findings had serum LDH activity and isoenzyme analysis within 24 hours of bronchoscopy
Monitoring for HIV-1, HIV-2, HTLV-I sero-progression and sero-conversion.: Int Conf AIDS 1989 Jun 4-9; 5:487 (abstract no. B.538); Constantine N, Fox E, Rodier G, Abbatte E; US Naval Medical Research Unit No. 3 (A WHO Collaborating Center for AIDS), Cairo, Egypt; To serologically monitor individuals whose sera had previously exhibited reactivity by at least one of seven retroviral screening assays, for sero-progression and sero-conversion for HIV-1, HIV-2, and HTLV-I. Sera from 33 high risk individuals in Djibouti were collected and re-examined after four mo
Eplblot-HIV Western blot as supplemental assay in testing HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:487 (abstract no. B.539); Kaltwasser G, Streeter M, Quero S, Montiel F; Catholic University of Chile, Faculty of Medicine, Laboratory of Clinical Microbiology-CEDIUC, Chile; We evaluated the EPlblot-HIV Western blot from Epitope Inc., Beaverton, Oregon USA as supplemental test for serum samples from patients suspicious of HIV infection. This Western blot test was used in a total of serum 70 samples: 65 were from patients whose EIA screening tests gave repeatedly positive results; 5 samples
Increased ADA activity in children with perinatal HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:487 (abstract no. B.540); Palomba E, David O, Gabiano C, Tovo PA; Instituto di Clinica Pediatrica, Universita' di Torino, Torino, Italy; To evaluate whether adenosine deaminase (ADA) activity is enhanced in erythrocytes of children with perinatal HIV infection and its possible prognostic significance. ADA was assessed, according to Hopkinson s method in RBC of 33 children born from HIV + mothers (8 P2, 13 P1, 12 PO) and in a control
[Quantitative Western blot: it's value in surveillance of HIV seropositive patients]: Int Conf AIDS 1989 Jun 4-9; 5:487 (abstract no. B.541); Schiavini D, Puel J; Laboratoire de Virologie, CHU Purpan, Toulouse, France
Model system in HIV-antibody testing for Boehringer Mannheim Enzymun-Test diagnostics.: Int Conf AIDS 1989 Jun 4-9; 5:487 (abstract no. B.542); Bayer H, Lin M, Klein C, Kirch P; Boehringer Mannheim GmbH, Forschungszentrum, D-8132 Tutzing, FRG; Screening tests have to be sensitive for possibly all specificities of HIV-antibodies, while specific antibody tests are important for prognosis of diseases (p24) or distinguishing HIV-I from HIV-II-infection. For our automatic Boehringer Mannheim Enzymun-Test-System we developed a model-system using a single universal
OKT4 epitope deficiency and symptomatic immune impairment mimicking aids.: Int Conf AIDS 1989 Jun 4-9; 5:488 (abstract no. B.543); Van de Perre P, Lepage P; Programme National de Lutte contre le SIDA, Kigali, Rwanda; CASE REPORT: The patient was a 14-year old girl presenting with disseminated cutaneous candidiasis , severe pain on left foot and persistent diarrhea. A X-ray film of the left foot revealed an osteitis lesion of the calcaneum suggestive of tuberculosis . Massive infestation of stool samples by Giardia lamblia was di
Peripheral blood lymphocytes (PBL) forming rosettes with their autologous erythrocytes (AE)-(AELFR) in HIV infected patients.: Int Conf AIDS 1989 Jun 4-9; 5:488 (abstract no. B.544); Pathouli C, Spiliotopoulou J, Kontopoulou-Griva I; Athens University, Athens, Greece; We observed that a small part of normal PBL 8-14% forms spontaneous rosettes (R) with their AE in Man. We wanted to investigate this phenomenon in individuals infected by HIV. We studied 23 Hemophiliacs positive for HIV and 22 individuals infected by HIV and/or suffering from AIDS. PBL were separate
Multidisciplinary approach to HIV seropositive patients; a two year experience of an out-patient department.: Int Conf AIDS 1989 Jun 4-9; 5:488 (abstract no. B.545); Barbarini G, Maccabruni A, Malfitano A, Calderon W, Parisi A, Tacconi F; Clinic of Infectious Diseases, IRCCS S. Matteo, University of Pavia, Italy; To give a complete response to increasing needs of HIV seropositive subjects. Since 1986 in the Clinic of Infectious Disease of Pavia, a special out-come patients department was established for ambulatory management of HIV infected subjects. In this structure are always present an infectivologist, a
[A rare cause of diarrhea in an HIV positive patient]: Int Conf AIDS 1989 Jun 4-9; 5:488 (abstract no. B.546); Annick D, Similowski T, Rosenheim M, Katlama C, Fassin D, Gentilini M; Dpt de Sante Publique, Parasitologie et Medecine Tropicale et Unite INSERM 313, Paris, France
Multiplication of HHV-6 and HIV1 in blood lymphocyte culture.: Int Conf AIDS 1989 Jun 4-9; 5:488 (abstract no. B.547); Agut H, Kerouedan D, M'Pele P, Collandre H, Rosenheim M, Huraux JM; Laboratoire de Bacteriologie-Virologie, Hopital de la Pitie-Salpetriere, Paris, France; To detect the simultaneous multiplication of HIV and human herpes virus-6 (HHV-6) in primary lymphocyte culture from patient s blood. Peripheral blood mononuclear cells (PBMC) were grown in primary culture according to conventional procedures. Secondary cultures were performed with freshly stimulate
A synthetic peptide ELISA for detection of antibodies to HIV1.: Int Conf AIDS 1989 Jun 4-9; 5:488 (abstract no. B.548); Libonatti O, Torstensson R, Mhalu S, Biberfeld G, Wigzell H; Karolinska Institute, Stockholm, Sweden; To synthesize and evaluate a peptide to be used in a diagnostic immunoassay. Hydrophilicity and the presence of two cysteines in close proximity were determinants to select a conserved sequence among different isolates of HIV1 derived from the transmembrane glycoprotein gp41. A twelve aminoacid pept
Concordance of polymerase chain reaction (PCR) with HIV antibody (Ab) in HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:489 (abstract no. B.549); Horsburgh CR, Ou CY, Jason J, Holmberg S, Evatt B, Jaffe H; AIDS Program, CDC, Atlanta, GA, USA; To evaluate the correlation of PCR detection of HIV DNA and detection of HIV Ab. We examined peripheral blood mononuclear cells (PBMC) and serum from 112 homosexual men, 85 hemophilic men, 30 HIV-infected transfusion recipients, and 91 heterosexual partners of HIV-infected persons in 5 prospective s
A retrospective analysis of diseases associated with indeterminate HIV Western blot patterns.: Int Conf AIDS 1989 Jun 4-9; 5:489 (abstract no. B.550); Drabick JJ, Horning L, Lennox J, Coyne P, Knight R, Dillard T, Burke D, Fuller S, Damato J; Walter Reed Army Medical Center, Washington, DC, USA; Some individuals possess antibodies reactive to HIV Western blot proteins in patterns not diagnostic of HIV infection. A retrospective chart review of patients exhibiting such antibodies was performed and compared to a control cohort of sex and age matched individuals from the same base population of HIV-negative blots
Etiology of indeterminate HIV Western blots.: Int Conf AIDS 1989 Jun 4-9; 5:489 (abstract no. B.551); Celum C, Coombs RW, Lafferty WE, Egan R, Sninsky J, Holmes KK; Univ of Washington, WA, USA; To ascertain the etiologies of indeterminate HIV Western blots (IWB), the rates of seroconversion and sexual transmission, and the yield of supplemental HIV diagnostic tests. Thirty-seven individuals with positive HIV ELISAs and IWB and their current sexual partners were enrolled from diverse HIV t
Use of vitreous humour of the eye for HIV antibody tests of medicolegal autopsies.: Int Conf AIDS 1989 Jun 4-9; 5:489 (abstract no. B.552); Kantanen ML, Brummer-Korvenkontio H, Karhunen PJ, Penttila A, Leinikki P; National Public Health Institute and Department of Forensic Medicine, University of Helsinki, Helsinki, Finland; In a HIV surveillance study all medicolegal autopsies have been tested in Helsinki area (N=3558). Since serum is often hemolysed and difficult to run in the standard tests, we tried other fluids for sample material. To evaluate vitreous humour as sample material for HIV antibody tests of medicole
Results of neutralization assays and use of the VRL correction factor with the Abbott and DuPont p24 antigen ELISAs.: Int Conf AIDS 1989 Jun 4-9; 5:489 (abstract no. B.553); Arens M, Storch G; Washington University School of Medicine, St. Louis, Missouri, USA; We compared DuPont (NEK046) and Abbott p24 antigen ELISAs using 35 serum samples found to be positive for p24 antigen by 1 or both assays. Of the 35, 34 were positive (greater than 10 pg/ml) by DuPont and 27 were positive by Abbott. However, 12 of the samples initially positive by DuPont failed to neutralize using DuPo
False-negative results in HIV-detection assays due to sample distribution.: Int Conf AIDS 1989 Jun 4-9; 5:489 (abstract no. B.554); Buimovici-Klein E, Lange M; St. Luke's-Roosevelt Hospital Center, Columbia University, New York, New York, USA; Only a small proportion of lymphocytes carry virus in HIV-infected persons. This proportion might be related to the severity of the disease. The number of HIV carrying lymphocytes necessary to elicit a positive response in virus culture assays is unknown, but is likely to be higher than one. Due to the Poiss
HIV antigenemia in patients with AIDS and HIV infection: a comparison between racial groups.: Int Conf AIDS 1989 Jun 4-9; 5:490 (abstract no. B.555); Hendricksen C, Farzadegan H, Bartlett J, Quinn T, Chaisson RE; The Johns Hopkins University, Baltimore, Maryland, USA; To determine if HIV p24 antigen(Ag) differs in white and black American patients, as has been suggested in African and European patients. 193 consecutive patients with AIDS or HIV infection who presented for clinical trials were studied; 164 had evaluable results. HIV-Ag was measured by the Abbott E
Traditional EIA and rapid screening for HIV1 confirmed by combined testing for antibodies and antigens.: Int Conf AIDS 1989 Jun 4-9; 5:490 (abstract no. B.556); Castriciano S, Calvert C, Mahony J, Chernesky M; McMaster University Regional Virology Laboratory, St. Joseph's Hospital, Hamilton, Canada; A total of 14 sera from well defined patient infections (5 AIDS, 1 ARC, 4 asymptomatic; 4 negative) were used to evaluate 15 commercial kits for HIV antibodies and another 3 for antigens. Antibody tests included 6 EIA s (Abbott, Organon, Dupont, Genetic Systems, Wellcome and Cambridge), 3 Western bl
[Absence of seroconversion after primary infection of HIV meninigitis]: Int Conf AIDS 1989 Jun 4-9; 5:490 (abstract no. B.557); Patey O, Dublanchet A, Israel G, Dormont D, Lafaix C; Service des maladies infectieuses et tropicales, France
[The retrovirus manifested in the carrier is semi-quantifiable in the mononuclear cells of the blood]: Int Conf AIDS 1989 Jun 4-9; 5:490 (abstract no. B.558); Mathez D, Paul D, Saurin W, Leibowitch J; Hospital Raymond-Poincare/Universite Rene-Descartes, Garches/Paris-ouest, France
Interest of computed tomography of the chest (C-CTS) in 120 HIV-infected patients (pts) with respiratory symptoms (RS).: Int Conf AIDS 1989 Jun 4-9; 5:490 (abstract no. B.559); Carette MF, Denis M, Penaud D, Mayaud C, Akoun G, Bigot JM; Depts Pneumology, Radiology - hopital Tenon - Paris - France; We evaluated prospectively the interest of C-CTS in 120 HIV-infected pts with RS leading to pulmonary investigations. All C-CTS were performed in high resolution (1 mm - 512 x 512) and were studied with knowledge of clinical features; data were compared with final diagnosis. First data showed that we had to consider 2
A sensitive and rapid method to detect histoplasma.: Int Conf AIDS 1989 Jun 4-9; 5:490 (abstract no. B.560); Lomvardias S, Vaidya KP; Department of Pathology, Woodhull Medical and Mental Health Center, Brooklyn, New York 11206, USA; To report a sensitive and rapid method for the identification of Histoplasma in tissues and in body fluids of AIDS patients. MATERIAL: Over a period of 18 months we examined 12 specimens from 7 patients. There were 6 alveolar lavages, 2 bone marrow aspirates, 2 peripheral blood smears, 1 liver biopsy and one
[Quantification of anti-p25 HIV by densitometry of the recombinant genetic antigen]: Int Conf AIDS 1989 Jun 4-9; 5:491 (abstract no. B.561); Noel L, Harzic M, Guerin MC, Maisonneuve P, Ghnassia JC, Saint-Paul B; Centre de Transfusion et d'Hematologie, Centre Hospitalier de Versailles, France
A sensitive and specific anti HIV-2 ELISA using an env-derived synthetic peptide.: Int Conf AIDS 1989 Jun 4-9; 5:491 (abstract no. B.562); Krupka U, Brust St, Behringwerke AG; 3550 Marburg, FRG; To evaluate the efficiency of an ELISA (Enzygnost-Anti-HIV-2), based on a synthetic peptide corresponding to the gp41 protein of HIV-2. 50 mu1 undiluted specimen are pipetted to 50 mu1 diluent into each well of microtitration plates coated with 1 synthetic peptide. The incubation over 30 at 37 degre
Study of infections in 58 AIDS autopsies from 1985 to 1988 in Rio de Janeiro, Brazil.: Int Conf AIDS 1989 Jun 4-9; 5:491 (abstract no. B.563); Basilio-de-Oliveira CA, Valle HA, Fialho F, Quinhoes EP, Miguez LA, Morais-de-Sa CA; Gaffree & Guinle University Hospital, University of Rio de Janeiro (UNI-RIO), Brazil; To demonstrate the main infectious conditions found among 58 autopsies of AIDS patients, and compare the findings among risk groups. The staining techniques used were: Hemathoxilin-eosin, PAS, Grocott and Reticulin. TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: 1. Toxoplasmosis was the mo
Role of HIV in human oral cavity.: Int Conf AIDS 1989 Jun 4-9; 5:491 (abstract no. B.564); Pekovic D, Garzon S, Strykowski H, Adamkiewicz VW, Ajdukovic D; AIDS Research Center, Biomedco Canada Inc., Quebec, Canada; Oral health status deteriorates drastically in AIDS patients. The present studies aim at correlating incidences of the presence of HIV in peripheral blood lymphocytes (PBL), gingiva and salivary lymphocytes (SL) with incidences of oral disease. HIV was searched for in PBL, gingiva and SL obtained from 96 patients at di
Primary malignant lymphomas of the central nervous system in HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:491 (abstract no. B.565); Sacchi G, Zorzi F, Baronchelli C, Bonetti M, Falchetti M, Stellini R; Service of Pathology, Spedali Civili Brescia, Italy; To describe the incidence of primary malignant non Hodgkin lymphomas (NHL) of the Central Nervous System (CNS) in HIV positive patients. The data concern 65 autopsies of full-blown AIDS pts. and 10 stereotaxic biopsies from pts. with expansive endocranial processes with problems of differential diag
Neuropsychological effects of early HIV-1 infection: diagnostic and methodological issues.: Int Conf AIDS 1989 Jun 4-9; 5:491 (abstract no. B.566); Ingraham L, Bridge P, Janssen R, Stover E, Mirsky A, Goodwin F; NIMH Intramural, Bethesda, MD, USA; To recommend a common methodological approach for neuropsychological assessment in HIV-1 infected individuals to foster meaningful comparisons among studies. Methods used in studies of neuropsychological performance in HIV-1 seropositive individuals were reviewed. Despite considerable metho
The incidence of a marker for cholinergic dysfunction in AIDS/ARC.: Int Conf AIDS 1989 Jun 4-9; 5:492 (abstract no. B.568); Van Gorp W, Tulin S, Evans G, Satz P, Freeman D, Hinkin C; Veteran's Administration, West Los Angeles, and UCLA School of Medicine, CA, USA; A specific pattern on the Wechsler Adult Intelligence Scale -- Revised (WAIS-R) associated with cholinergic dysfunction has been reported by Fuld to occur in 50% of patients with Alzheimer s disease and in 50% of younger individuals given scopolamine, while occurring in only 10% of patients with multi- infar
HIV antibody and antigen in the CSF of an HIV-infected child with progressive spastic paraparesis and cognitive defects, treated by intraventricular administration of gammaglobulin.: Int Conf AIDS 1989 Jun 4-9; 5:493 (abstract no. B.573); Calvelli T, Belman A, Chowdhury M, Williams P, Vazquez W, Rubinstein A; Albert Einstein College of Medicine, Bronx, NY, USA; To monitor fluctuations in HIV antibody and antigen in the CSF of a perinatally infected child receiving intraventricular gammaglobulin for the treatment of HIV-associated neurologic defects. An Omaya reservior to deliver intraventricular hyperimmune gamma-globulin was inserted at age 3 1/2 (1986).
Neurodevelopment of infants with perinatally-acquired HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:493 (abstract no. B.574); Wachtel RC, Holden W, Nair P, Black M, Gratz E, Johnson J; University of Maryland School of Medicine, Baltimore, MD, USA; To determine the effect of HIV infection upon the developing nervous system. Twenty infants born to English speaking HIV positive intravenous drug abusing mothers were followed prospectively from birth as part of an ongoing study of perinatal HIV infection. All infants were evaluated every three mon
Movement disorders in children with perinatal HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:493 (abstract no. B.575); Kaplan M, Bedell G; State University of New York, Health Science Center at Brooklyn, Brooklyn, New York, USA; To describe specific movement problems observed in children (age birth to five) with HIV infection and appropriate treatment goals and priorities. 22 children admitted to the ICLC since January, 1988 were evaluated by occupational therapists using observation, criterion referenced evaluation and sta
Periodontal diseases in HIV-positive patients is more related to the number of T-4 cells than to specific microorganisms.: Int Conf AIDS 1989 Jun 4-9; 5:493 (abstract no. B.576); Lucht E, Heimdahl A, Nord CE, Bratt G, Berglund O; Dept of Oral Surgery, Karolinska Institute, Stockholm, Sweden; Gingival inflammation (GI) and periodontal disease (PD) were compared in 10 patients with HIV infections without clinical symptoms, 10 patients with ARC, 10 patients with AIDS and 10 healthy non-infected sex and age matched controls. All subjects were investigated regarding supragingival dental plaque, subgingival micr
Proposal for international perinatal AIDS protocols.: Int Conf AIDS 1989 Jun 4-9; 5:493 (abstract no. B.577); Kline J, Stein Z; HIV Center for Clinical and Behavioral Studies, New York, NY, USA; To facilitate international co-operation and co-ordination of research in perinatal AIDS. Analyses of reported studies are presented, showing that differences in sample selection, case definition, clinical description, and laboratory procedures limit the pooling of findings from different sites. RE
Do anti-sperm antibodies interfere with HIV antibody tests?: Int Conf AIDS 1989 Jun 4-9; 5:493 (abstract no. B.578); Ferrera C, Nielsen IO, Berger RE, Krieger MS, Keiger JN; University of Washington, Seattle, Washington, USA; 1. To determine whether anti-sperm antibodies interfere with HIV-1 antibody test results. 2. To evaluate the performance of a 10-minute, peptide-based antibody test for HIV done in a physician s office. Sera from patients undergoing infertility evaluation were tested for anti-sperm antibodies (Isoj
Thrombotic thrombocytopenic purpura (TTP) in an HIV infected child: treatment with 1-desamino-8-D-arginine(DDAVP) and intravenous immunoglobulin (IVIG).: Int Conf AIDS 1989 Jun 4-9; 5:494 (abstract no. B.579); Church JA, Marshall G, Laug W; Childrens Hospital of Los Angeles, Los Angeles, CA, USA; To describe therapy of TTP in an HIV-infected child. METHOD: Case report. After 2 weeks of upper-respiratory infection-symptoms, a 6 1/2 year old boy with Down Syndrome and transfusion-associated HIV infection presented with acute hemolytic anemia. Previous hemograms and blood chemistries were norma
Giant cell transformation of the liver in pediatric AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:494 (abstract no. B.580); Kahn E, Daum F, Greco MA, Magid M, Morecki R, Anderson V; North Shore Univ. Hosp., Manhasset-Cornell Univ. Med. College, NY, USA; Emphasize that giant cell transformation occurs in the liver of children with HIV infection. Review of liver tissue of 46 children with AIDS (biopsies=7, autopsies=42). Giant cell transformation of the liver was seen at autopsy in 4 of 46 children. All 4 had parental IV drug abuse as their
Mucocutaneous Kaposi's sarcoma: a marker of paediatric AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:494 (abstract no. B.581); Mbidde EK, Banura C, Kizito A; Uganda Cancer Institute (UCI), Uganda; To describe clinical features of childhood Kaposi s sarcoma (KS) that are diagnostic of AIDS. Case records of all children admitted to the UCI with diagnosis of KS between June 1987 and January 1989, were reviewed for features that are suggestive of paediatric AIDS. Evaluation of these children incl
Clinical manifestations in African children perinatally infected by HIV and aged 5 years old or more.: Int Conf AIDS 1989 Jun 4-9; 5:494 (abstract no. B.582); Lepage P, Van de Perre P, Hitimana DG, Van Goethem C, Kestelyn P, Nsengumuremyi F; Department of Pediatrics, Center Hospitalier de Kigali, Programme National de Lutte contre le SIDA, Kigali, Rwanda; To describe the clinical features of perinatally HIV-infected Rwandese children surviving over 5 years of age. Sixteen HIV-seropositive children born to HIV-infected mothers, aged 5 to 12 years old (9 girls and 7 boys; mean age 6.5 years) and diagnosed between 1984 and 1987 in Kigali,
Ranula cysts in children with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:494 (abstract no. B.583); Anderson VM, Lee H, Greco MA, Arroyo A; SUNY Health Science Center, Interfaith Medical Center, Brooklyn, NY, USA; OBJECT: To report the clinicopathologic correlations of ranula cysts in children with AIDS. METHOD: Two cases are reported: 1) Patient 1 had lymphadenopathy and lymphocytic interstitial pneumonitis (LIP) at age 1 year and a 3cm in diameter, sublingual, ranula cyst which was excised at age 2. Within 3 weeks it reoccurre
Thymic pathology in pediatric AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:494 (abstract no. B.584); Greco MA, Thomas P, Mahnovski V, Magid M, Kahn E, Anderson V; New York University, NY, NY, USA; A variety of thymic lesions have been reported in AIDS. This more detailed and systematic description expands the previous reports. We examined the thymuses of 19 infants and children with AIDS aged 2.5 mos to 5.5 years, at autopsy. One infant had a normal thymic shadow on chest roentgenogram at 4 m
Immunocompetent cells in livers of children with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:495 (abstract no. B.585); Thomas P, Greco MA, Kahn E, Magid M, Mahnovski V, Anderson V; New York University, NY, NY, USA; To define the inflammatory cells, especially macrophages and antigen presenting cells (APCs) in livers of children dying of AIDS. METHOD: Seventeen livers from autopsies of AIDS children aged 2.5 mos to 7 yrs and sections from 11 non-AIDS controls were studied by immunohistochemistry. All had hepatomegaly an
Comparison of immunoglobulins and growth paramenters at birth among HIV seropositive and seronegative infants.: Int Conf AIDS 1989 Jun 4-9; 5:495 (abstract no. B.586); Butz A, Hutton N, Larson E, Joyner M, Vogelhut J, McHugh J; Johns Hopkins University, Baltimore, Maryland, USA; To examine growth and biological data at birth in infants born to HIV seropositive and seronegative mothers in high risk groups (IV drug users (IVDA), sex partners of IVDA). Twenty-three infants born to high risk women, enrolled in a 15 month longitudinal study, were examined at birth for immunoglob
Treatment of HIV drug addicts with IFN alpha 2 recombinant: preliminary report.: Int Conf AIDS 1989 Jun 4-9; 5:495 (abstract no. B.587); Orlando G, Belletti G, Gennaro M, Papa D, Puppo F, Indiveri F; Infectious Disease Dept, USL 2 Sanremo, Italy; 18 HIV positive patients (16 drug addicts) classified according Walter Reed s classification as 14-WR2; 1-WR3; 2-WR5; and 1-WR6 have been treated since 1987 according to the following scheme: IFN alpha 2a (Roferon) 3xMU/die consecutively for 6 days and for 6 weeks subcutaneously. Toxicology, collateral effects
Non-myelosuppressive chemotherapy for HIV-associated aggressive lymphoma.: Int Conf AIDS 1989 Jun 4-9; 5:495 (abstract no. B.588); Kennedy P, Murray C, Scolaro M; Los Angeles Oncologic Institute, Los Angeles, California, USA; To demonstrate the efficacy and toxicity of Bleomycin, Oncovin, and Prednisone (BOP) in combination with AZT and Acyclovir in treatment of HIV infected patients with aggressive lymphoma .
[Aggressive chemotherapy of bronchopulmonary lesions of Kaposi's sarcoma accompanying acute respiratory insufficiency]: Int Conf AIDS 1989 Jun 4-9; 5:495 (abstract no. B.589); Denis M, H'Moud T, L'Huillier JP, Parquin F, Mayaud C, Akoun G; Centre de Pneumologie et de Reanimation Respiratoire - Hopital TENNON - 75020 Paris - France
Does HLA background influence the rate of perinatal transmission of HIV?.: Int Conf AIDS 1989 Jun 4-9; 5:495 (abstract no. B.590); Hague R, Kilpatrick D, Mok J, Yap PL, Coutts NA; City Hospital, Edinburgh, UK; To determine whether there is any association between HLA class I and II types and the development of HIV infection in infants born to HIV seropositive mothers. 8 HIV seropositive symptomatic children (age 2-5) were studied for HLA A,B and DR types together with 25 children age 1-5 all of whom were
EFFICACY AND SAFETY OF KETOCONAZOLE IN HIV INFECTED INFANTS WITH mucocutaneous candidiasis.: Int Conf AIDS 1989 Jun 4-9; 5:496 (abstract no. B.591); LeMay MD, Cooper ER, Patel DK, Pelton SI; Boston City Hospital and Boston University School of Medicine, Boston, Massachusetts, USA; Mucocutaneous candidiasis is a frequent opportunistic infection in children with HIV disease and associated with significant morbidity (pain, poor feeding, failure to thrive). Recommended therapies such as Nystatin and gentian violet have not been efficacious in immunocompromised hosts with moderate to severe candidias
HIV-1 gp 41 antigen in the serum of HIV infected children.: Int Conf AIDS 1989 Jun 4-9; 5:496 (abstract no. B.592); Ellaurie M, Calvelli T, Rubinstein A; Albert Einstein College of Medicine, Bronx, NY, USA; To detect the presence of HIV-1 gp 41 antigen in the sera of HIV-infected children and correlate with disease activity. HIV-1 gp 41 antigen (Ag) was assayed by an immunoblot assay involving reaction with monoclonal gp 41 antibody followed by peroxidase-conjugated goat anti-mouse IgG. A positive reac
Which AIDS patients need step down care? A prospective study.: Int Conf AIDS 1989 Jun 4-9; 5:496 (abstract no. B.593); McCormick WC, Inui TS, Deyo RA, deGroff C, Wood R; University of Washington, Seattle VA Medical Center, Seattle, WA, USA; To assist in discharge planning for hospitalized AIDS patients in need of special AIDS residences with skilled nursing ( step down care ), we carried out a prospective study of 100 persons with AIDS to determine the characteristics of patients in need of such care, and to develop a discriminant rule to easi
An early intervention program for children with HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:496 (abstract no. B.595); Leonard N, Kaplan M, Sloan L, Harvell I, Calev A, Bedell G; SUNY Health Science Center at Brooklyn, NY, USA; To describe a program for developmentally delayed (DD), HIV infected children and to evaluate the children s performance. The Infant and Child Learning Center (ICLC) provides a comprehensive educational, therapeutic and psychosocial program. Each child and caregiver attends a small class several tim
Controlled clinical trial of anginlyc, Chinese herbal immune enhancer, in HIV seropositives.: Int Conf AIDS 1989 Jun 4-9; 5:496 (abstract no. B.596); Sankary T; AIDS Prevention Center, San Francisco, California, USA; To assess the safety and efficacy of a new Chinese herbal immune enhancer developed in the People s Republic of China , which is produced from extracts of angelica, ginseng, and fructus lycium, in the treatment of early to mid-HIV infection. 300 HIV seropositive patients with T4 helper lymphocyte co
[Effectiveness and tolerance of treatment with zidovudine in patients with ARC or AIDS]: Int Conf AIDS 1989 Jun 4-9; 5:497 (abstract no. B.597); Sette P, Camporiondo MP, Narciso P, Tossini G, Tozzi V, Visco G; U.S.L. RM/10 Hop. "L. Spallanzani" Dep.Mal.Infect. Rome, Italie
[Preliminary results of treatment of c. neoformans meningoencephalitis in Burundi]: Int Conf AIDS 1989 Jun 4-9; 5:497 (abstract no. B.599); Floch JJ, Laroche R, Deppner MD, Kadenede P, Sauniere JF; Faculte de medecine de Bujumbura, Burundi
The treatment of intercurrent processes for children with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:497 (abstract no. B.600); Cilleruelo MJ, Mellado MJ, Villota J, Barreiro G, Garcia M, Fontelos PM; Instituto Carlos III, Serv. Pediatria, Madrid, Spain; We selected 11 children, whose ages ranged from 2 months to 11 years, who suffer sympthomatic HIV infection. All of them are included in P2 class of the CDC and present intercurrent processes: 100% mucocutaneous candidiasis , 100% upper respiratory infections (S. Aureus, Candida and H. Influenzae), 80% malnutrition, 63
Adverse drug reactions in AIDS patients.: Int Conf AIDS 1989 Jun 4-9; 5:497 (abstract no. B.601); Iribarren JA, Arrizabalaga J, Garde C, Barcia MJ, Rodriguez-Arrondo F, Zulaica D; Infectious Diseases Unit and Pharmacy Department, Hospital Nuestra Senora de Aranzazu, San Sebastian, Spain; Appraisal of the incidence and severity of adverse reactions to drugs [ARD] in AIDS patients. A prospective evaluation was made of the ARD that occurred in 112 patients who were diagnosed as AIDS in our hospital between 1985 and December 1988. The number of secondary hospital admissions, drugs respo
Cloning and expression of HIV-I and HIV-II proteins with diagnostic purposes.: Int Conf AIDS 1989 Jun 4-9; 5:497 (abstract no. B.602); Garcia J, Novoa LI, Machado JA, Benitez J, Padron G, Herrera L; Center of Genetic Engineering and Biotechnology, C. Habana, Cuba; To obtain HIV-I and HIV-II viral antigen using genetic engineering techniques. Representative regions of the genes coding for the gag 24, gp 41, gp 160 HIV-I and gp 36 HIV-II proteins were inserted into a plasmid under the control of tryptophan promoter and the ligation products were transformed in
HIV immunoblot patterns and p24 antigen detection in sequential sera of homosexual men.: Int Conf AIDS 1989 Jun 4-9; 5:498 (abstract no. B.603); Read S, Major C, McLaughlin B, Coates R, Calzavara L, Fanning M; University of Toronto, Toronto, Canada; Sequential sera, collected every 3 months over a 3 year period from 15 HIV seroconverters and 21 individuals who have developed AIDS during the study period, had antibody patterns examined by immunoblot and were tested for p24 antigen. In the seroconverter group, one individual showed p24 antigen prior to the detection
Detection of HIV antigen by fluometric enzyme immunoassay.: Int Conf AIDS 1989 Jun 4-9; 5:498 (abstract no. B.605); Hiroshi U, Asano M, Honma H, Matsuno S, Morikawa S, Komatsu T, Kitamura T; AIDS Research Center, National Institute of Health, Japan; Detecting HIV antigens is more important to find HIV infection in earlier stages and to the safety of blood and blood products. Mouse MoAbs against p24 of LAV, DEAE-Cellulose-purified anti-HIV rabbit IgG and column-purified anti-HIV human IgG from HIV carriers were used. A microplate was coated with
The effect of zidovudine on platelet counts in HIV positive individuals.: Int Conf AIDS 1989 Jun 4-9; 5:498 (abstract no. B.606); Montaner J, Le T, Gelmon K, Fanning M, Falutz J, Tsoukas C, Wells G, O'Shaughnessy M, Wainberg M, Ruedy J, et al; Multicentre Canadian Azidothymidine Trial (MCAT); To characterize the nature, time course and dose dependency of zidovudine effect on platelet counts in HIV infected individuals within the Multicentre Canadian AZT Trial. Design: Multicentre, prospective, dose range finding study. Subjects: 57 HIV positive homosexual men; CDC groups IIB, III and IVC
Administration of aerosolized pentamidine to the homeless AIDS population in a non-traditional setting/shelter.: Int Conf AIDS 1989 Jun 4-9; 5:498 (abstract no. B.607); Vinitsky S, McMahon J; University of Massachusetts, Boston, Massachusetts, USA; To determine the number of homeless AIDS patients living at the City of Boston s Long Island Shelter who would benefit from prophylactic treatment of aerosolized Pentamidine (A.P.) at the shelter level. METHOD: The identification of the clients needing prophylaxis has been obtained from Boston City Hospital
Inhibition of sexual drive in HIV carriers by medroxyprogesterone acetate.: Int Conf AIDS 1989 Jun 4-9; 5:498 (abstract no. B.608); DiGiovanni C, Berlin F; Metropolitan Psychiatric Group, Washington, DC, USA; To report further clinical experience with medroxyprogesterone acetate (MPA) as an inhibitor of sexual drive in HIV carriers. Since our original report in 1987 of the use of MPA in one sexually promiscuous HIV carrier, we have treated 7 additional patients. All were young gay men who were seropositi
HIV-infections among i.v.- drugabusers in the FRG: prevalence-rates, differential risks and proportions of behavior change of addicts.: Int Conf AIDS 1989 Jun 4-9; 5:499 (abstract no. B.609); Kleiber D; Sozialpadagogisches Institut Berlin, Berlin (West), FRG; Our results of a German (nearly) representative differential- epidemiological study (N=630) to estimate the actual HIV - prevalence-rate and the relative riskiness of needle-sharing and sexual activities among i.v. drugabusers show a much smaller percentage of infected persons than expected (20, 1% HIV-positive). A hig
Incidence of AIDS and other infections in Spanish drug addicts analysis of 8660 cases after a decade of follow-up (1977-87).: Int Conf AIDS 1989 Jun 4-9; 5:499 (abstract no. B.610); [No authors listed]; Spain; To study the prevalence and the characteristics of AIDS and other infections in Spanish parenteral drug addicts (PDA). Fifty eight Spanish hospitals reported, using a standardized form, all infectious complications in PDA who required hospital admission or hospital control as outpatients. The diagno
Rapid progression of organic delusional syndrome to dementia in AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:499 (abstract no. B.611); Holmes VF; State University of New York at Stony Brook, Stony Brook, New York, USA; To clinically assess the course of Organic Delusional Syndrome in patients with AIDS. Hospitalized AIDS patients referred to the Stony Brook University Hospital Psychiatric Consultation Service were assessed by a board certified psychiatrist utilizing medical chart review and semi- structured interv
Clinical characterisation of HIV2 disease in West Africa.: Int Conf AIDS 1989 Jun 4-9; 5:499 (abstract no. B.612); Tumani Corrah Egboga AA, Wilkins AB, Gaye I, Whittle H, Greenwood B; Medical Research Council, Fajara, The Gambia; Particular attention is paid to the pattern of secondary infections in patients with HIV2 disease. All cases suspected of having AIDS, AIDS selected complex or progressive generalised lymphaderopathy were included in the study. Serological diagnosis was made by Harvard methods prior to testing in o
HIV infection - immunological studies in Bulgaria.: Int Conf AIDS 1989 Jun 4-9; 5:499 (abstract no. B.613); Plotchev K, Taskov H, Vasilev T, Ognjanov M, Dikov I, Doganov B; Institute for Infectious Diseases, Sofia, Bulgaria; For a period of three years we have been studying the immunological status of 50 men infected with HIV /proved by ELISA and Western blot. 3 of them have AIDS, 7 slight clinical disturbances and 40 are carriers of HIV. 22 out of these 50 men with HIV infection have signs of disfunction in the immune system: impaired cel
[Clinical observations of HIV infections in Bulgaria]: Int Conf AIDS 1989 Jun 4-9; 5:499 (abstract no. B.614); Dikov I, Douhovnikova T, Charakchieva R, Djelebova J, Kotcheva R, Plotchev K; Clinique des Maladies Infectieuses, Sofia, Bulgarie
Pulmonary hemorrhage in pneumonitis of HIV-infected patients.: Int Conf AIDS 1989 Jun 4-9; 5:500 (abstract no. B.615); Brechot JM, Dore MF, Capron F, Waked M, Rochemaure J; Department of Pneumology, Paris, France; Study the frequency of pulmonary hemorrhage (PH) in pneumonitis of HIV-infected patients by scoring alveolar macrophage hemosiderin load as described by Golde (Golde s score GS). On 16 of the consecutive 134 bronchoalveolar lavage (BAL), PH was diagnosed with a GS greater than 100 (mean value=125) a
Hemoptysis complicating Pneumocystis carinii pneumonia.: Int Conf AIDS 1989 Jun 4-9; 5:500 (abstract no. B.616); Vaidya KP, Lomvardias S; Dept. of Pathology, Woodhull Medical & Mental Health Center, Brooklyn, New York, USA; To describe pulmonary destruction with hemorrhage associated with Pneumocystis carinii (PC) pneumonia. Case Report. A 54 year old Hispanic female intravenous drug addict presented with hemoptysis of two days duration, fever, chills and productive cough. She had cachexia, tachypnea, generalized lymphadenopath
[Study of the specificity of antibodies detected in subjects during the course of seroconversion with the help of recombinant proteins]: Int Conf AIDS 1989 Jun 4-9; 5:500 (abstract no. B.617); Gadelle S, Crabbe C, Lesieur C, Delagneau JF; Diagnostics Pasteur, Marnes (France)
Evaluation of an ultrasensitive ELISA for specific detection of anti HIV 1.: Int Conf AIDS 1989 Jun 4-9; 5:500 (abstract no. B.618); Brust S, Krupka U; Behringwerke AG, 3550 Marburg, FRG; To evaluate an improved competitive ELISA for the detection of antibodies against HIV-1. 100 mu1 undiluted specimens are pipetted to 25 mu1 diluent buffer in each well of microtitration plates coated with cell culture propagated HIV-1 antigens. The first incubation step over 60 min at 37 degrees C i
Efficacy of Abbott recombinant HIV-1/HIV-2 EIA for early detection of HIV-2 and/or HIV-1 seroconversion.: Int Conf AIDS 1989 Jun 4-9; 5:500 (abstract no. B.619); Ferchal F, Agbalika F, Honderlick P, Barateau V, Perol Y; Laboratoire de Bacteriologie-Virologie, Hopital St-Louis, Paris, France; The greatest difficulty in HIV diagnosis in Europe is to have at one s disposal an ELISA test sensitive enough to detect low levels of HIV-2 as well as HIV-1 antibodies. We determined the performance characteristics of Abbott recombinant HIV-1/HIV-2 EIA by studying sequential sera from one HIV-2 and seven HIV-1 serocon
Detection of serum antibodies against only the gag proteins: a latent or an abortive infection with an HIV variant?: Int Conf AIDS 1989 Jun 4-9; 5:500 (abstract no. B.620); Theodoropoulou M, Malliarakis N, Spanakis E, Malliaraki-Pinetidou E, Georgoulias V; AIDS Reference Center of Crete, Venizelion Hospital, Iraklion, Greece; To determine whether heterosexuals displaying antibodies against only the gag proteins are really HIV-infected. Peripheral blood lymphocytes (PBL) were co-cultured with the HIV- permissive CEM leukemia cell line and virus replication was evaluated by the reverse transcriptase activity (RT) and the p
Simultaneous three color detection of human T cells (CD2, CD4 and CD8) by flow cytometry.: Int Conf AIDS 1989 Jun 4-9; 5:501 (abstract no. B.621); Bergeron M, Mandy F, O'Shaughnessy VM; Bureau of Laboratories and Research Services, Federal Centre for AIDS, Health Protection Branch, Ottawa, Ontario, Canada; To obtain rapid results with T cells markers utilizing 0.1 mL of blood. By choosing some optical filter and dye configurations on a flow cytometer that has 3 color- photmultiplier tubes (PMTs). METHOD: A Coulter EPICS Profile (PROFILE) was configured with 3 log. fluorescence PMTs. The optical bench had three
Anti-HIV test of third generation (evaluation of sensitivity by HIV seroconversion).: Int Conf AIDS 1989 Jun 4-9; 5:501 (abstract no. B.622); Joller-Jemelka HI, Joller PW, Parkhede U; University Hospital, Zurich, Switzerland; Sera were available of 15 individuals before anti-HIV screening test became positive (seroconversion). HIV antigen (p24) became detectable before seroconversion in all 15 individuals. As early as HIV-Ag, IgM anti-HIV also became detectable as measured by Western blot but these antibodies persisted longer. In 10 individ
Diagnosis of HIV-1 infection in African couples: comparison of serology and PCR.: Int Conf AIDS 1989 Jun 4-9; 5:501 (abstract no. B.623); Vaira D, Sondag Thull D, Francois Gerard C, Doppagne A, Rentier B; AIDS Reference Laboratory, Universite de Liege, Belgium; Search for the rate of HIV-1 contamination among seronegative sexual partners of seropositive individuals. Classical serological methods (EIA, WB) and PCR. SERIES: 36 heterosexual couples from central Africa, accounting for a total of 73 persons: 13/37 seropositive women, 23/36 seropositive men. All
Proposal for the anti HIV testing protocol.: Int Conf AIDS 1989 Jun 4-9; 5:501 (abstract no. B.624); Krupka U, Brust S; Behringwerke AG, 3550 Marburg, FRG; To propose a strategy for testing anti HIV. In view of new assays for the simultaneous detection of anti HIV-1 and -2 a second ELISA level might be useful for the differentiation and confirmation of positive or equivocal results obtained in the initial level of combined screening. The peptide based
HIV IgG antibody at the age of 12 or 18 months but not HIV IgM antibody at birth or age 3 months correlates with clinical evidence of perinatally-acquired HIV infection (PI).: Int Conf AIDS 1989 Jun 4-9; 5:501 (abstract no. B.625); Ryder R, Nsa W, Francis H, Hassig S, Behets F, Vercauteren G, Piot P, Quinn T; Proj SIDA, Kinshasa, Zaire; To determine the correlations between detection of HIV IgM antibody (cord blood, age 3 mos.) and HIV IgG antibody (12 mos., 18 mos.) and the development of clinical evidence of PI. Two groups of children have been followed since birth. Group I children had 373 children with HIV(+) mothers. Group II
An in vitro EIAV RT model for screening of anti-HIV agents.: Int Conf AIDS 1989 Jun 4-9; 5:501 (abstract no. B.626); Pei-zhen T, Xing-quan Z, Ke-jiu Q; Institute of Medicinal Biotechnology, CAMS, Beijing, PR China; To establish an in vitro model for HIV using reverse transcriptase (RT) assay of equine infectious anemia virus (EIAV). Virus was concentrated from tissue cultures and disrupted. EIAV RT assay was directed by (rA)n.(dT)(I2-18) with normal substrate dTTP (specific activity, 5070ccpm/pmol). Several 5
Correlation of sexual behavior and antisperm antibodies in homosexual men.: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.627); Mulhall BP, Carter L, Clark S, Harrison L, Fieldhouse S, Short R; Burnet Clinical Research Unit, Walter & Eliza Hall Institute and AIDS Service, Royal Melbourne Hospital, Australia; To detect antisperm antibodies in the serum of homosexual men and their relation to sexual practices, with a view to the development of a screening test that would act as a epidemiological marker for the practice of anal sex. Using information obtained from a questionnaire, 60 homosexual volunteers
Value of Ig class and IgG subclass detection in the serum and CSF as a prognostic means in HIV infected patients.: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.628); Tamalet C, Rousseau S, Raoult D, Gallais H, Romette J, Tamalet J; Hopital de la Timone, Marseille, France; To estimate the prognostic value of the IgA, IgM, and IgG subclass specific antibodies in the sera and CSF of HIV infected patients. Sera and CSF samples were obtained from 15 HIV infected patients (7 AIDS,4 ARC,4 Asymptomatic). Ig class and IgG subclass specific antibodies were identified by Wester
[Simultaneous screening of HIV1 and HIV2 infections in France using a combination test]: Int Conf AIDS 1989 Jun 4-9; 5:502 (abstract no. B.629); Cot MC, Gadelle S, Brun-Vezinet F, Courouce AM, Foucault C, Janot C; Diagnostics Pasteur, Marnes, France
Tax: its role in the diagnosis of HTLV I infection.: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.630); Cerney M, Monplaisir N, Nguyen M, Osame M, Sonoda S, Lee H; Abbott Laboratories, North Chicago, IL, 60064, USA; To evaluate the diagnostic potential of antibody to the HTLVI tax protein (p40X). Blood was collected from 619 Martinique donors, 25 IV drug abusers in the U.S., 33 TSP/HAM and 25 ATL patients. Samples were screened by two types of EIA; the first using HTLVI virus lysate as the capture antigen, the
Seroprevalence of HTLV-I and HIV-1 in adult hematologic oncology patients.: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.631); Feischer HE, Lichtiger B, Reuben J; University of Texas, MD Anderson Cancer Center, Houston, TX, USA; To determine the seroprevalence of HTLV-I and HIV-1 in cancer patients with hematologic malignancies. Sera from patients with hematologic malignancies were assayed by ELISA for the presence of antibodies to both HTLV-I and HIV-1 using the FDA-approved, commercially available kits. Patients categorie
Natural history of HTLV-I infection.: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.633); Tochibana N, Tsuda K, Yoshida M, Essex M, Mueller N; Miyazaki Medical School, Miyazaki, Japan; To describe the natural history of HTLV-I infection within an endemic area of Kyushu, Japan . A population based cohort established of approximately 1687 adult residents of two villages, of whom 505 are HTLV-I positive. HTLV-I antibody titer was determined by PA and proviral DNA in carriers lymphocy
Prevalence of HTLV I/II infection in blood donors and high risk groups in the U.S.: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.634); Steaffens J, Palker T, Krochmal E, Mann T, Osame M, Lee H; Abbott Laboratories, North Chicago, IL, USA; To determine the prevalence of HTLVI exposure among plasmapheresis donors and high risk populations in the U.S. Specimens from IV drug abusers (IVDA), blood donors and high risk groups such as hemophiliacs and homosexuals were collected between 9/87 and 10/88 and tested for antibodies to HTLVI by EI
Dual assay of HTLV-I and HIV-1 antibody using anti-FC EIA with recombinant HIV-1 and HTLV-I antigens.: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.635); Hung CH, Zeigler W, Tang L, Thorn RM, Beltz GA, Marciani DJ; Cambridge BioScience Corporation, Worcester, MA, USA; To develop a recombinant antigen immunoassay (EIA) that has the capacity to detect both HIV-1 and HTLV-I antibodies in human sera. Affinity-purified goat anti-human IgG Fc and anti-human IgM FC were coated on microtiter plates to capture human antibodies from test serum. Virus specific antibodies we
Detection of early HTLV-I seroconversion (SC).: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.636); Manns A, Murphy E, Wilks R, Hanchard B, Drummond J, Waters D, Swanson P, Lee H, Blattner W; NCI, Bethesda, MD, USA; Evaluate sensitivity of HTLV-I screening and confirmatory assays during SC among transfusion recipients (REC). Pre-transfusion negative exposed REC from Jamaica , followed at monthly intervals, were tested by whole virus (wv) (DuPont and Abbott) and recombinant envelope (env) (Cambridge Biosci.) EIA
Focus of HTLV-1 associated tropical spastic paraparesis in Equateur, Zaire: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.637); Kazadi K, Desmyter J, Goubau P, Carton H; University Hospital, Kinshasa, Zaire; To present the first focus of HTLV-I associated tropical spastic paraparesis (TSP) in Africa. Only 5 sporadic cases are known in Africans, although Africa has the largest world reservoir of HTLV-I. Search among chronic paraparesis patients from Lisala, population 50,000, Equateur Province,
HIV p24 antigen in asymptomatic, PGL and AIDS patients at Gaffree & Guinle University Hospital, Rio de Janeiro, Brazil.: Int Conf AIDS 1989 Jun 4-9; 5:503 (abstract no. B.638); Sion FS, Salerme-Goncalves R, Quinhoes EP, Graca MH, Morais de Sa CA, Castilho EA; Gaffree & Guinle University Hospital, Rio de Janeiro University (UNI-RIO), Brazil; To determine the presence of p24 antigen in patients with different clinical status of HIV infection. Serum HIV p24 antigen measured by antigen capture ELISA ( Abbott Laboratories , North Chicago, IL, USA) was performed in 125 patients who met the surveillance case definition of HIV infection by Cen
Intracutaneous tests with ubiquitous antigens and CD4-CD8 determinations in AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:504 (abstract no. B.639); Zeballos R, Cavalcante N, Freire C, Lewi D, Accetturi C, Mendes N; Escola Paulista de Medicina, Sao Paulo, Brazil; To correlate simple immunological parameters with the clinical classification of AIDS. A total of 127 AIDS patients classified according to CDC criteria were tested in vivo with PPD, candidin, trichophytin and streptokinase- streptodornase; the absolute numbers of CD4 and CD8 lymphocytes were also d
Predictive value of the determination of HIV-1 Ag in serum.: Int Conf AIDS 1989 Jun 4-9; 5:504 (abstract no. B.640); Leal M, Pineda JA, Calderon E, Navarro MD, Rey C, Lissen E; University of Seville, Seville, Spain; To assess the predictive value of the determination of HIV-1 Ag in serum. We have tested for HIV-1 Ag by EIA 212 serum samples sequentially collected from 51 anti-HIV-1 positive patients. On an average, they had been followed during 42.10 11.9 months (range 24-73) and 4.15 1.11 (range 3-9) serum sam
[Clinical aspects of HIV1 and HIV2 and classification by Bangui]: Int Conf AIDS 1989 Jun 4-9; 5:504 (abstract no. B.641); Sow A, Coll AM, Fayendao MA, Diouf G, Feller-Dansokho E, Diop BM; Service des Maladies Infectieuses, C.H.U. de Dakar
[Study of the diagnostic value of Western blot in 113 Congolese]: Int Conf AIDS 1989 Jun 4-9; 5:504 (abstract no. B.642); Rosenheim M, Kerouedan D, M'Pele P, Copin N, Yala F, Itoua-Ngaporo A, Gentilini M; Dpt de Medecine Tropicale, Hopital Salpetriere et Unite INSERM 313, 47 Bd de l'Hopital, Paris, France
Detection of human immunodeficiency virus (HIV)DNA sequences in seropositive patients and seronegative at risk subjects by polymerase chain reaction (PCR).: Int Conf AIDS 1989 Jun 4-9; 5:504 (abstract no. B.643); Ensoli F, Fiorelli V, Mezzaroma I, Auiti F; Dept. of Allergy and Clinical Immunology, University of Rome, Rome, Italy; To investigate the earlier stage of the infection we detected HIV-1 DNA sequences in seronegative people at high risk as drug addicts and partners of seropositive subjects and seropositive patients classified according to CDC criteria. Normal blood donors were employed as controls. Genomic DNA s col
[Quality control of screening and confirmation tests of anti-HIV antibodies in 1988]: Int Conf AIDS 1989 Jun 4-9; 5:504 (abstract no. B.644); Mouillot L, Francois E, Cheiney I, Courouce AM; Laboratoire National de la Sante Departement de Biologie Medicale Paris France
The use of graphic art to measure neuropsychological function in HIV-infected persons.: Int Conf AIDS 1989 Jun 4-9; 5:505 (abstract no. B.645); Spechko P, Peabody B; UCSD Medical Center, San Diego, CA, USA; To measure neuropsychological function in people with HIV infection through their artwork. Twice- weekly, 3 hour sessions for HIV-infected persons for art therapy/ instruction generated written and photographic observations of artwork and behavior of 75 patients over 4 1/2 years. In an unstructured,
Outcome of advanced maternal AIDS in offspring.: Int Conf AIDS 1989 Jun 4-9; 5:505 (abstract no. B.646); Davochi F, Kabena M; Department of Pediatrics, Mama Yemo Hospital, Kinshasa, Zaire; To define the outcome of advanced maternal AIDS in offspring. We followed 20 infants born to mothers with advanced clinical AIDS. Eighteen mothers had produced 2 twins and 16 singles. Mothers were all ill at the time of delivery. Four died within 4 weeks of giving birth. Two had
Adverse reactions to trimethoprim-sulfamethoxazole (TMP-SMX) treatment of Pneumocystis carinii pneumonia (PCP) in AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:505 (abstract no. B.647); Gonzalez-Lahoz J, Moreno V, Polo R, Martinez R, Adrados M, Aguado CG; Instituto de Salud Carlos III, Hospital del Rey, Servicio de Medicina Interna-I; Toxic effects of TMP-SMX treatment of PCP are frequent in American-white patients with AIDS. Better tolerance to this treatment has been reported in non-American AIDS patients. Our experience is reported here. 43 patients with HIV infection and a first episode of PCP diagnosed by bronchoscopy with bronchoalveo
Adverse effects of zidovudine (AZT) in patients with HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:505 (abstract no. B.648); Valencia ME, Pintado V, Frances M, Lavilla P, Dupla JM, Gil A; Hospital La Paz, Madrid, Spain; Assesment of adverse effects (AE) to zidovudine in patients with HIV infection in an intensive pharmacologic surveillance program. MATERIAL AND 33 patients were entered. 22 patients fulfilled AIDS criteria, 3 patients had a group III (CDC) infection, 3 group IV-E infection and 3 group IV-C2 infectio
Inhibition of retrovirus-induced diseases by two naturally occurring polycyclic aromatic diones hypericin and pseudohypericin.: Int Conf AIDS 1989 Jun 4-9; 5:642 (abstract no. C.501); Lavie G, Meruelo D; Dept. of Pathology, New York University School of Medicine, New York, NY USA; Two aromatic polycyclic dione compounds hypericin (HY) and pseudohypericin (PS) have been found to markedly inhibit the onset and progression of retrovirus induced disease. The compounds were originally derived from plants of the hypericum family (St. Johnswort). Their intravenous administration to mice infected with F
Transmission of SIVmac to baboons.: Int Conf AIDS 1989 Jun 4-9; 5:642 (abstract no. C.502); Cranage M, Baskerville A, Stott E, Kitchin P; PHLS CAMR, Salisbury SP4 OJG, UK; To study the transmissibility and course of infection of freshly isolated SIVmac in baboons and rhesus macaques. Virus was isolated from a rhesus macaque 57 days after intravenous inoculation with SIVmac251lP (from Dr Desrosiers) by co-cultivation of monkey PBL with human cord blood lymphocytes. Thi
Differing effects of helper T lymphocyte depletion on murine infection with herpes simplex (HSV) and cytomegalovirus (MCMV).: Int Conf AIDS 1989 Jun 4-9; 5:642 (abstract no. C.504); Mills J, Erlich K, Wofsy D, Shanley J; Univ. of Calif., San Francisco, CA, USA; To determine effect of helper-inducer T lymphocyte depletion on the pathogenesis of HSV and MCMV infection of mice. Injection of rat monoclonal antibody GK1.5 into mice causes greater than 95% depletion of L3T4 lymphocytes (the murine homologue of CD4 lymphocytes). In this model, resistance
Identification and characterization of rabbit CD4 using antisera prepared against human CD4.: Int Conf AIDS 1989 Jun 4-9; 5:643 (abstract no. C.508); Gordon MR, Kindt TJ; Laboratory of Immunogenetics, NIAID, NIH, Bethesda, MD 20892; USA; To prepare and characterize antisera which bind rabbit CD4 and to identify T-cell subpopulations in normal and HIV-1 infected rabbits. Goats, sheep and chickens were each immunized subcutaneously with 80 ug of recombinant soluble human CD4 (gift of D. Capon, Genentech Inc.) in 0.1 ml complete Fr
Development of a neutralizing antibody assay for the simian immunodeficiency virus model.: Int Conf AIDS 1989 Jun 4-9; 5:643 (abstract no. C.510); Maxfield L, Jennings M, Holmes E, Carlson J; Dept. of Medical Pathology, University of California, Davis, USA; To develop a neutralization antibody assay (Nt assay) that will be used to monitor SIV infected monkeys. Four cell lines (Hut 78, MT-2, MT-4 and CemX174) were infected with a standard stock of SIV supernatant and monitored for infection by Reverse Transcriptase assay (RTA), P24 antigen assay (Dupont
Role of ethanol-induced immunosuppression on disease and tumor resistance of retrovirally infected mice.: Int Conf AIDS 1989 Jun 4-9; 5:643 (abstract no. C.511); Darban HR, Huang DS, Watson RR; University of Arizona, Tucson, Arizona, USA 85724; To develop a murine model of AIDS for study of immunomodulatory cofactors as reviewed in Life Sci. 44:i, 1989. To determine the effects of a murine retrovirus causing immunosuppression, alone and with dietary ethanol, on cellular immune functions. Then to assess the effects of immunosuppression on infectious
Development of murine retrovirus model using F(1) hybrid mice for evaluation of therapeutic substances.: Int Conf AIDS 1989 Jun 4-9; 5:643 (abstract no. C.512); Morrey JD, Warren RP, Burger RA, Okleberry KM, Sidwell RW; AIDS Research Program, Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan, Utah USA; To develop a predictive murine model for AIDS using an F(1) hybrid mouse strain capable of eliciting viral specific neutralizing antibodies that reduce retrovirus titers near limits of detection, but which still die of the disease, and to use this model for evaluating potential anti-AIDS therapies.
A new simplified assay for neutralizing antibodies to HIV-1.: Int Conf AIDS 1989 Jun 4-9; 5:644 (abstract no. C.513); Weislow O, Kiser R, Fowler A, Arthur L, Nara P, Boyd M; Program Resources, Inc., NCI-Frederick Cancer Research Facility, Frederick, MD, USA; To develop a simple, large-scale assay for the safe and rapid quantitation of antibodies which block HIV-1 infectivity. The reduction of a new tetrazolium salt, XTT, by viable T-lymphoblastoid cells to a soluble formazan was used to quantitate HIV cytopathic effects in a semi-automated 5-day microcu
Antibody dependent cell-mediated cytotoxicity (ADCC) in relation to clinical progression of HIV-1 associated disease in haemophiliacs.: Int Conf AIDS 1989 Jun 4-9; 5:644 (abstract no. C.514); Sinclair AL, Habeshaw J, Muir L, Steele M, Ludlam C, Dalgleish AG; MRC Clinical Research Centre, Harrow, UK; Evaluation of the ability of serial serum samples from a combat of HIV-1 positive haemophiliacs to mediate ADCC, with study of the relationship of ADCC to clinical state and disease progression. METHOD: HIV-1 isolates in the CEM cell line were used as target cells and peripheral blood mononuclear cells from
Antisperm antibodies as a risk factor for sexually transmitted HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:644 (abstract no. C.515); Witkin SS, Jeremias J; Cornell University Medical College, New York, New York USA; To determine whether men with antisperm antibodies in their semen will be more likely than men who are not sensitized to sperm to transmit HIV to their sexual partners. Men with a history of STDs and homosexual men have a high incidence of antibodies to sperm. Sperm from fresh ejaculates were tested
[Modulation of CD4 expression by phorbol myristate acetate and HIV infection in vitro]: Int Conf AIDS 1989 Jun 4-9; 5:644 (abstract no. C.516); Sanhadji K, Bernard I, Touraine JL; Faculte de Medecine A. Carrel, INSERM U80-CNRS URA 1177, Lyon, France
Quantification of HIV-Binding to CD4+cells using flow cytometry.: Int Conf AIDS 1989 Jun 4-9; 5:644 (abstract no. C.517); Thiele B, Kunze RO, Gebauer F, Ramirez G, Gelderblom H, Pauli G; Abt. Virologie des Robert Koch-Instituts und AIDS-Zentrum, BGA-Berlin, Germany; The anti-CD4 antibody Leu 3a is able to inhibit HIV-viron binding to its receptor, the CD4 molecule. (Weiss et al., 1984). On the other hand, the viral protein gp 120 can block Leu 3a antibody binding. Titration of fluorescein-conjugated Leu 3a in combination with Scatchard-Plot analysis of flow cytometric data gives a
Spontaneous INF-alpha production by alveolar macrophages and peripheral blood monocytes from patients with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:644 (abstract no. C.518); Krishnan V, Mitchell D, Pinching AJ; St. Mary's Hospital Medical School, London, UK; To assess spontaneous and LPS induced TNF-alpha production by monocytes/macrophages from AIDS patients. METHOD: Bronchoalveolar lavage (BAL) cells and peripheral blood mononuclear cells (PMBC) were cultured for 24 hours, with and without LPS (1 ug/ml), after which supernatants were collected and assayed for
Induction of major histocompatibility (MHC) Class II antigen on cells of the developing human fetal peripheral nervous system by gamma-interferon.: Int Conf AIDS 1989 Jun 4-9; 5:645 (abstract no. C.519); Boyer BA, Wigdahl B; Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033 USA; To examine the effect of gamma interferon (gamma-IFN) on the level of MHC class II expression on cells isolated from the developing human fetal peripheral nervous system. gamma-IFN was used to study the induction of MHC class II antigen expression on the surface of cells obtained from dorsal root ga
Decreasing antibody responses in association with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:645 (abstract no. C.520); Hendry RM, Katzenstein D, McCutchan JA, Sawyer LA, Vujcic LK, Quinnan GV; LRR, DV, Center for Biologics Evaluation and Research, FDA, Bethesda, MD, USA; To identify HIV specific immune responses associated with the development of AIDS in HIV seropositive homosexual men. Sera were obtained semi-annually over 3 years from 20 asymptomatic HIV seropositive men in San Diego, 10 of whom developed AIDS and 10 remained stable. They were studied by quantitat
The effect of HIV on the expression of HLA-DR and cytochrome-b oxidase mRNA in mononuclear cells.: Int Conf AIDS 1989 Jun 4-9; 5:645 (abstract no. C.521); Tobin EH, Remold H; Harvard Medical School and Brigham and Women's Hospital, Boston, MA, USA; To determine if HIV infection can alter the expression of two distinct mRNA markers of mononuclear cell activation: 1. HLA-DR--the major histocompatibility antigen responsible for accessory cell function; and 2. Cytochrome b heavy chain (cyt-b)--a component of the oxidase system responsible for mononuclear c
Early development of anti-HIV IgG.: Int Conf AIDS 1989 Jun 4-9; 5:645 (abstract no. C.523); Wahren B, Trojnar J, Solver E, Albert J, Sandstrom E, Broliden PA, Wigzell H; National Bacteriological Laboratory, Stockholm, Sweden; To enhance the early seroreactivity in HIV infection/immunization. Non-glycosylated 15-30 aa peptides covering the CD4 binding region of HIV were prepared by solid phase synthesis. All peptides were made to contain two cysteine residues in the same molecule and chemically oxidized. The sulphur bridg
The immune response to HIV-1 envelope protein involves both ADCC and CTL.: Int Conf AIDS 1989 Jun 4-9; 5:645 (abstract no. C.524); Riviere Y, Tanneau F, McChesney M, Cordonnier A, Emerman M, Kieny MP, Sansonetti P, Montagnier L; Institut Pasteur, Paris, France; env protein specific cytotoxic effector cells, present in the peripheral blood of most HIV seropositive donors, have been characterized as either CD8+, MHC restricted or CD8- and MHC unrestricted. Phenotypic studies and analysis of antigen processing were used to define these effector cells. Uncleav
Tumor Necrosis Factor (TNF) stimulates HIV1 transcription in human T cells.: Int Conf AIDS 1989 Jun 4-9; 5:646 (abstract no. C.525); Israel N, Hazan U, Alcami J, Bachelerie F, Arenzana-Seisdedos F, Virelizier JL; Institut Pasteur, Immunologie Virale, Paris, France; We have examined whether TNF might stimulate HIV1 transcription and we have analyzed the mechanism involved. METHODS AND We transfected J. Jhan cell line (a subclone from Jurkat T cell line) with the plasmid LTR HIV.CAT which contains the HIV1 LTR sequences upstream of the bacterial CAT gene. CAT ac
HIV-1 and HIV-2 specific IgG subclass reactivity to env and gag peptides.: Int Conf AIDS 1989 Jun 4-9; 5:646 (abstract no. C.526); Broliden PA, Outtara AS, Solver E, Trojnar J, Norrby E, Wahren B; National Bacteriological Laboratory, Stockholm, Sweden; To evaluate and compare the HIV-1 and -2 specific IgG subclass reactivity to selected peptides. Peptides representing five immunodominant regions of the HIV-1 and -2 env and gag proteins were used as antigens in IgG subclass ELISA assays. Sera from HIV-1 and -2 infected individuals were analysed. RE
Relationships between the physiological function of CD4 and its role as the HIV receptor.: Int Conf AIDS 1989 Jun 4-9; 5:646 (abstract no. C.528); Sattentau QJ, Merkenschlager M, Healey D, Truneh A, Arthos J, Sweet R, Beverley PC; The academic Dept. of G-U Medicine, UCMSM, London, UK; To define with the use of monoclonal antibodies (mAbs) to CD4 and their anti-idiotypes those sites on the CD4 antigen important in the physiological functions of this molecule in relation to their role in HIV reception. The amino acid residues essential for the binding of a number of CD4 mAbs has b
High levels of cell-associated IL-1 in circulating monocytes from HIV-infected individuals.: Int Conf AIDS 1989 Jun 4-9; 5:646 (abstract no. C.529); Weiss L, Haeffner-Cavaillon N, Laude M, Kazatchkine MD; Unite d'Immunopathologie, Hospital Broussais, Paris, France; Stimulation of normal monocytes results in transcription of IL-1 alpha and beta genes and accumulation of cell-associated IL-1. Some but not all IL-1 inducers further trigger the extracellular release of IL-1 beta. We have examined the production of cell-associated IL-1 in vivo and that of cell-associated and extracell
Specificity of CD4+ T cells from cynomolgus macaques immunized with hybrid HIV P24:Ty virus like particles.: Int Conf AIDS 1989 Jun 4-9; 5:646 (abstract no. C.530); Mills K, Barnard A, Adams S, Kingsman S, Kingsman A, Kitchin P; NIBSC, Blanche Lane, South Mimms, Herts EN6 3QG, UK; To assess the ability of a novel antigen presenting system to generate T-cell responses to HIV gag proteins in primates. Four pairs of cynomolgus macaques were immunized with a recombinant HIV-1 gag protein expressed in yeast as hybrid P24:Ty virus like particles (VLPs). Animals received 3 i.m. inje
T cell recognition of HIV peptides.: Int Conf AIDS 1989 Jun 4-9; 5:647 (abstract no. C.531); Schrier RD, Wiley CA, Gnann JW Jr, Landes R, Lockshin C, Richman D, McCutchan A, Oldstone MB, Nelson JA; Research Institute of Scripps Clinic, La Jolla, CA, University of California, San Diego, La Jolla, San Diego VA Medical Center, San Diego, USA; Because T-cell responses are critical for defense against viral infections, a series of synthetic peptides derived from the predicted sequence for human immunodeficiency virus (HIV-1) proteins gp41, gp 120, gag and viral polymerase were employed to test the T-cell proliferative response of HIV-1 seropositive individual
Study of the function and structure of IL2-R in HIV-infected subjects.: Int Conf AIDS 1989 Jun 4-9; 5:647 (abstract no. C.535); Lunardi-Iskandar Y, Spanakis E, Jasmin C, Georgoulias V; Oncogenese Appliquee -INSERM U268-, Hop. Paul Brousse, 14-16 av. P.V. Couturier, g4804 Villejuif, France; To elucidate the mechanisms responsible for the low proliferative responses of T cells during HIV infection. PBL were obtained from 7 AIDS, 5 LAS and 6 asymptomatic seropositive patients as well as from 6 normal controls. Surface Tac expression was studied by indirect immunofluorescence whereas the
Impairment of human neutrophil (PMN) function in vitro by HIV-1 and GP 160.: Int Conf AIDS 1989 Jun 4-9; 5:647 (abstract no. C.536); Bai XO, Numazaki K, Lyras A, Wainberg MA, Mills EL; Lady Davis Institute for Medical Research, Montreal, Quebec, Canada; In addition to opportunistic infections, individuals (especially children) infected with HIV-1 may also be at increased risk for bacterial infections due to pyogenic organisms such as S.aureus. The PMN of HIV-infected persons have been reported to have impaired bactericidal activity and chemotaxis but a normal respirat
Anti-idiotypic antisera to anti-CD4: their specificity and anti-HIV potential.: Int Conf AIDS 1989 Jun 4-9; 5:648 (abstract no. C.538); Healey D, Broadhurst K, Beverley P, Sattentau Q; Middlesex Hospital, London, UK; To investigate the cross-reactive nature of anti-idiotypic reagents to Leu3a anti-CD4 and to evaluate their potential to inhibit syncytial formation between CD4+ c8166 cells and HIV RF. Lou rats, Balb/c and SJL mice were immunised with Leu3a anti-CD4 antibody coupled to KLH, administered intraperito
Preliminary evaluation of a recombinant SIV-envelope peptide for use as a diagnostic for HIV-2 infection.: Int Conf AIDS 1989 Jun 4-9; 5:648 (abstract no. C.540); Lennick M, Wilde A, Kanki PJ, Beltz GA; Cambridge BioScience Corporation, Worcester, MA, USA; To develop a recombinant peptide for use in an HIV-2 immunoassay. METHODS & We have cloned several unique restriction fragments from the SIV envelope coding region into E. coli expression vectors. When tested in Western blots, three of the expressed proteins were capable of detecting all of the
Bronchoalveolar lavage (BAL) mononuclear subset cells, at different stages of HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:648 (abstract no. C.541); May T, Kohler C, Amiel C, Faure G, Gerard H, Canton P; CHU - Nancy - France; Long term effects of HIV infection on BAL mononuclear cells were evaluated in a cohort of 88 patients, at different stages of HIV infection. These patients were divided in 4 groups, according to clinical stages: group A = CDC II and III; group B = ARC; group C = AIDS except pulmonary infections; gro
Analysis of expression of gamma delta T-cell receptor and CD3+CD4-CD8-phenotype by lymphocytes from HIV-1 seropositive and seronegative homosexual men in the Baltimore MACS.: Int Conf AIDS 1989 Jun 4-9; 5:648 (abstract no. C.542); Margolick J, Scott E, Saah A; The Johns Hopkins School of Hygiene and Public Health, Baltimore, MD, USA; To determine the degree to which the increase in calculated CD3+CD4-CD8- (double negative; DN) lymphocytes associated with HIV-1 seropositivity (Margolick et al., Clin. Immunol. Immunopathol., in press) is due to an increase in lymphocytes expressing the gamma delta T-cell receptor (TCR). Peripheral
Use of autologous CD4+ T cells in the assay of HIV-1-specific cytotoxic T cells (HIV-CTL).: Int Conf AIDS 1989 Jun 4-9; 5:649 (abstract no. C.543); Izaguirre CA, Drouin J; Department of Medicine, University of Ottawa and Ottawa General Hospital, Ottawa, Ontario, Canada; We have reported that the anti-HIV drugs castanospermine (CSP) and dextran sulfate (DS) induce the regeneration, within 5 days, of CD4+ T cells in lectin- stimulated cultures of blood lymphocytes from HIV+ patients. Based on this observation, we describe a method that permits the use of autologous CD4+ T cel
Chemical synthesis of chimeric peptides and proteins for immunological purposes.: Int Conf AIDS 1989 Jun 4-9; 5:649 (abstract no. C.544); Mascagni P, Sia DY, Toth GK, Gibbons WA, Haaheim LR, Kent S, Coates AR; The School of Pharmacy, London, UK; To chemically synthesize a 104 amino-acid long peptide corresponding to the 273-377 sequence C terminal half of HIV-1 gag p24, and the hybrid peptides p24 (T-epitope)-linker-env (B epitope) 42 mer and p24 (273-377)-linker-env (B-epitope) 138 mer. The three polypeptides were synthesised using highly
A chemically synthesized 104-mer peptide of the C-terminal half of HIV-1 P24 successfully used as an HIV immunogen.: Int Conf AIDS 1989 Jun 4-9; 5:649 (abstract no. C.545); Haaheim LR, Mascagni P, Sia DY, Tooth GK, Gibbons WA, Coates AR; The London Hospital Medical College, London, UK; To investigate the immunological properties of a chemically synthesised C-terminal half (104 residues) of HIV-1 p24 core protein. The 104-mer peptide was prepared using an Applied Biosystems 430-A synthesiser employing t-Boc chemistry and used in purified form in the subsequent work. T-cell response
MHC class I-III allotypes in patients with disseminated Kaposi sarcoma (KS), ARC/WR5, and in normal controls.: Int Conf AIDS 1989 Jun 4-9; 5:649 (abstract no. C.546); Plum G, Krickeberg H, Siebel E, Bendick C, Rasokat H, Mauff G; Depts. of Medical Microbiology, Virology, Internal Medicine II, Dermatology, Pathology, Biostatistics, Univ. of Cologne, FRG; Major histocompatibility complex (MHC) class I and II (=HLA-A,B,C,DR) association in HIV-infected patients has been controversial. Of the MHC coded complement components C2, BF, C4A/C4B (MHC class III) allotypes of C2 and C4 have not yet been reported. C4 allotypes appear of major immunogenetic relevance fo
Carbohydrate-dependent epitopes of HIV-1 GP120.: Int Conf AIDS 1989 Jun 4-9; 5:649 (abstract no. C.547); Bolmstedt A, Sjoblom I, Akerblom L, Olofsson S; Department of Clinical Virology, University of Goteborg, Goteborg, Sweden; Carbohydrates may decrease or increase the antigenic activity of viral glycoproteins. In the heavily glycosylated HIV-1 gp120 the carbohydrate content represents one half of the molecular weight. The aim of this study was to identify regions on gp120 where N-linked carbohydrates exert modulating effects on t
[Modification of the secretion of interleukin 1 by healthy epidermis in HIV infections]: Int Conf AIDS 1989 Jun 4-9; 5:650 (abstract no. C.549); Dreno B, Milpied B, Dutartre H, Litoux P; Clinique Dermatologique - Hotel Dieu - Pl. A. Ricordeau - 44035 Nantes Cedex 01 - France
Syncytium formation and destruction of CD4+ bystander cells cocultured with persistently HIV-infected T-cells, as demonstrated by flow cytometry.: Int Conf AIDS 1989 Jun 4-9; 5:650 (abstract no. C.550); Schols R, Pauwels R, Baba M, Desmyter J, De Clercq E; Rega Institute for Medical Research, K.U. Leuven, B-3000 Leuven, Belgium; To investigate cell fusion and giant cell formation following fusion between HIV-1- or HIV-2-infected (HUT-78) cells and uninfected CD4+ MOLT-4 (clone 8) cells, the two cell populations were mixed and cultured at 37 degrees C, stained with the appropriate monoclonal antibodies (mABs) (FITC- or PE-conjugated), fixed and
Mucosal immune system in HIV1 infection; activity of specific antibodies in parotid saliva.: Int Conf AIDS 1989 Jun 4-9; 5:645 (abstract no. C.552); Trainin Z, Archibald D, Furth P, Hamel DJ, Di S, McLane MF, Essex M; Harvard Univ., Boston, MA, USA; To look for qualitative and quantitative properties of the specific antibodies in the parotid saliva of HIVI infected people. Serum and parotid saliva was collected from 19 HIVI infected people of different clinical status; CDCII (7), CDCIII (3), CDCIV (9). The immunoglobulins IgA, IgG and IgM were
Naturally occurring auto-antibodies to CD4 and the MHC class II molecule in HIV-infected patients.: Int Conf AIDS 1989 Jun 4-9; 5:650 (abstract no. C.553); Drabick JJ, Baker J, Layne A, Sweet R, Sadoff J; Walter Reed Army Medical Center, Washington, DC., United States of America; The natural ligand for CD4 is the MHC Class II molecule. Antibodies to the CD4 binding site of HIV could theoretically cross-react with MHC Class II as both bind to CD4. Such anti- HIV binding site antibodies could also result in anti-idiotype antibodies cross-reactive with CD4. Western blots of EBV- transformed B cell
Cell-mediated lymphoproliferative responses by adults and children to recombinant HIV envelope and core peptides.: Int Conf AIDS 1989 Jun 4-9; 5:650 (abstract no. C.554); Borkowsky W, Moore T, Krasinski K, Papaevangelou V; New York University Medical Center and Bellevue Hospital Center, New York, USA; To determine if recombinant HIV antigens elicit cell-mediated immune responses in infected adults and children and to compare these responses to those of seronegative individuals. Lymphocytes from individuals at risk for HIV infection were cultured with recombinant HIV envelope and core (ENV & C
Antigenic analysis of HIV-1 major capsid protein, p24.: Int Conf AIDS 1989 Jun 4-9; 5:651 (abstract no. C.555); Parekh BS, Balasubramanian P, Link B; Clinical Division, Bio-Rad Laboratories, Hercules, CA 94547, USA; Although most HIV-1 seropositive individuals mount a significant immune response to p24, attempts to define one or more major antigenic sites on the molecule have not been successful. We report results of our experiments to identify antigenic site(s) on p24. Long synthetic peptides, 50 to 70 residue
Chemotactic activity of thymopentin (TP) on lymphocytes from patients with HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:651 (abstract no. C.556); Sampietro C, Torre D; Division of Infectious Diseases, Regional Hospital, 21100 Varese, Italy; To determine chemotactic activity of TP on lymphocytes from HIV-infected patients. Recently, we showed defective chemotaxis of lymphocytes in patients with ARC and AIDS. In addition, thymic involution occurs early in HIV infection. TP is a immunomodulating drug isolated from bovine thymus that induces T-cell
Anti-viral agents for AIDS: lymphocyte function as a marker of response.: Int Conf AIDS 1989 Jun 4-9; 5:651 (abstract no. C.557); Knox KA, Peters B, Pinching AJ; St. Mary's Hospital Medical School, London, UK; To examine the value of lymphocyte function as a marker of response to Zidovudine and Active Lipid 721 chemotherapy by monitoring PHA induced proliferation. Patient and control peripheral blood mononuclear cells were prepared by density gradient centrifugation, resuspended at 10(6) cells/ml and cult
Activation of complement by HIV-infected H9 cells.: Int Conf AIDS 1989 Jun 4-9; 5:651 (abstract no. C.558); Spear GT, June BA, Landay AL, Lint TF; Department of Immunology, Rush Presbyterian St. Lukes Medical Center, Chicago, Illinois, USA; To determine whether HIV-infected H9 cells activate complement (C) via the alternative (AP) or classical pathway (CP). H9 cells were infected with HIV(RF) or HIV(CDC451). For AP activation, cells were incubated with human AB+ serum as a C source for 30 min at 37 degree C and washed. The cells were t
Evaluation of the role of the soluble CD8 receptor and IL2 receptor (IL2R) in patients with Human Immunodeficiency Virus infection.: Int Conf AIDS 1989 Jun 4-9; 5:652 (abstract no. C.561); Liebes L, Chachoua A, McAuliffe V, Brown M, Kung P, Valentine F; New York University Medical Center, NY, NY 10012, USA; We evaluated the levels of soluble CD8 and IL2 receptors (IL2R) in patients with HIV infection. 63 patients with HIV infection and 7 normal controls were studied. Of these, 21 were asymptomatic seropositive (ASSYM), 19 had ARC, 13 had KS and 10 had AIDS with opportunistic infection. In previous stud
An interactive relational database for HIV and the immune system.: Int Conf AIDS 1989 Jun 4-9; 5:652 (abstract no. C.562); Hirons G, Stanley EA, Hyman JM, Nelson G; Los Alamos National Laboratory, Los Alamos, New Mexico 87545 USA; To organize the complex, changing information about HIV and the immune system in a visually oriented, user-friendly, interactive system. We are using HyperCard on an Apple Macintosh to create an interactive reference tool on HIV and the immune system. Information is presented on cards, each of which
Circulating interferon-gamma in HIV-1 infection.: Int Conf AIDS 1989 Jun 4-9; 5:652 (abstract no. C.563); Fuchs D, Hausen A, Reibnegger G, Werner ER, Dierich MP, Wachter H; University of Innsbruck and Ludwig Boltzmann Institute of AIDS Research, Innsbruck, Austria; Progressive HIV-1 infection is associated with diminished in vitro production of lymphokines by T-cells upon antigenic and mitogenic stimulation. However, in vivo markers, e.g. neopterin, indicate hyperproduction of certain cytokines. We measured serum concentrations of interferon gamma and neopteri
B endorphin, A.C.T.H. and cortisol relationship with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:652 (abstract no. C.564); Le Goaster J, Rougeot C, Tekaia F, Dray F, Montagnier L, Le Magnen J; Hopital Trousseau- Paris, France; Study of beta endorphin, A.C.T.H., cortisol plasma concentrations in homosexual HIV infected male groups. 72 non drug-abuser males, 20-35 years old. 3 homosexual groups: AIDS=25, SERO + =15, SERO- =15, one uninfected heterosexual group as controls n=16.2 blood samples daily (8am-5pm). beta endorphin
Suppressive effects of immunodeficiency viruses on the in vitro stimulation of human lymphocytes.: Int Conf AIDS 1989 Jun 4-9; 5:652 (abstract no. C.566); Lower J, Bonilla J, Konig H, Kurth R; Paul-Ehrlich-Institut, Frankfurt am Main, FRG; Animal retroviruses exert a suppressive effect on the in vitro stimulation of lymphocytes. The equivalent effect of primate immunodeficiency viruses (HIV-1, HIV-2, SIVmac, SIVagm) on human cells should be studied and the responsible viral components should be identified. In the lymphocyte stimulatio
Idiotypic reconstruction of HIV-1 gp41-gp120 interacting structures.: Int Conf AIDS 1989 Jun 4-9; 5:653 (abstract no. C.567); Siccardi AG, Andronico F, Erfle V; Dipartimento di Biologia e Genetica per le Scienze Mediche, Universita di Milano, Italia; To understand the function of cross-reactive epitopes shared by HIV-1 gp120 and a human lympho-monocytic activation protein. A monoclonal anti-idiotypic antibody (9G5A) raised against M38 (anti-gp120 and anti-HELP) binds and competes M38, but also binds a bacterial fusion protein carrying the extern
Differences in killing of CD4 cells induced by HIV isolates from asymptomatic and AIDS patients as measured by Cr51 cytotoxicity assay.: Int Conf AIDS 1989 Jun 4-9; 5:653 (abstract no. C.568); Schechter M, Valentine FT; Federal University, Rio de Janeiro, Brazil; New York University Medical Center, NY, USA; To examine differences in HIV-induced cell-contact mediated cell death using isolates from asymptomatic (AS) and AIDS patients (OI), employing a simple and reproducible assay. A cytotoxicity assay using as target cells Cr51 labeled CEM cells (CEM) or peripheral blood lymphocytes (PBL) from an uninfe
Quality assessment of HIV antibody tests in West Germany.: Int Conf AIDS 1989 Jun 4-9; 5:653 (abstract no. C.569); Enzensberger R, Doerr HW; University Clinics Frankfurt, Department of Medical Virology, FRG; Testing for HIV antibodies is subject to continuous technical improvement. Therefore permanent monitoring of the tests quality is a major requirement in HIV serology. A panel of ten sera (three HIV-positive, one HIV-2 positive and six HIV negative) were sent to 77 German laboratories for a quality control test. Serum s
In vitro lymphocyte proliferative responses (LPR) in HIV infected patients receiving zidovudine (ZVD).: Int Conf AIDS 1989 Jun 4-9; 5:653 (abstract no. C.570); Oksenhendler E, Rabian-Herzog C, Colombani J, Seligmann M; Hopital St Louis - Paris - France; Since clinical improvement is not associated with a persistent increase in CD4 cell counts, we have evaluated LPR upon ZVD therapy. 41 HIV seropositive patients (9 AIDS, 30 ARC and 11 with thrombocytopenia received ZVD with a daily dosage of 1170+/- 240 mg (800 - 1500). Evaluation of LPR was perform
Significance of non-lymphoid accessory cells in oral hairy leukoplakia.: Int Conf AIDS 1989 Jun 4-9; 5:653 (abstract no. C.571); Pimpinelli N, Romagnoli P, Ficarra G, Borgognoni L, Riccardi R, Gaglioti D, Milo D; University of Florence and U.S.L. 10/D, Florence, Italy; To provide further insight into the features of non-lymphoid accessory cells (NLCs), their relationships to lymphocytes, the influence of the epithelial microenvironment on their differentiation and their possible direct viral infection in oral hairy leukoplakia (OHL). We studied by immunohistochemi
Expression in E. coli, purification and immunological analysis of HTLV-1 ENV antigens.: Int Conf AIDS 1989 Jun 4-9; 5:653 (abstract no. C.572); Tribe DE, Ganfield MC, Lischwe MA, Tritch R, Chamberlain M; University of Melbourne, Melbourne, Australia; Segments of the HTLV-1 env gene were cloned in E. coli expression vectors. The segments expressed contained both gp46 and p21E determinants. Good yields of an insoluble env-fusion protein were achieved using a trp expression vector. This insoluble antigen was readily purified from disrupted cells by repeated washings a
Lymphocyte stimulation in ARC/WR5 patients during intravenous immunoglobulin (IVIG) treatment.: Int Conf AIDS 1989 Jun 4-9; 5:654 (abstract no. C.573); Krickeberg H, Plum G, Mertens T, Mauff G; Departments of Medical Microbiology, Virology, Internal Medicine II, Dermatology, Pathology, Biostatistics, Univ. of Cologne, FRG; In a randomized, controlled double blind study 15 ARC/WR5 patients were compared during 6 months 0.4g/kg IVIG treatment with 15 placebo treated patients. The project has been described elsewhere (cf.: Schrappe-Bacher et al.). Our study was aimed at lymphocyte response to T- and B-cell mitogens. (3)
"Active lipids" (AL) in vitro: loss of detectable T- and B-cell surface antigen.: Int Conf AIDS 1989 Jun 4-9; 5:654 (abstract no. C.574); Bogner JR, Matuschke A, Heinrich B, Fuessl HS, Goebel F; Universitat Munchen, Med Poliklinik, FRG; AL have been shown in vivo to increase the percentage of lymphocytes detectable neither by T- nor B-cell markers = unstained cells (UNST) (Stockholm 3531). We have investigated if this effect can also be observed in vitro. METHOD: Blood from patients with AIDS (n=4) and healthy controls (n=3) was incubated w
Immuno-adherence of mycobacterium avium (M. avium) to U(937) is enhanced by HIV-1 infection.: Int Conf AIDS 1989 Jun 4-9; 5:654 (abstract no. C.575); Bakker LJ, Nottet HS, Van Strijp JA, Visser MR, Snippe H, Verhoef J; Lab. of Microbiology, State University of Utrecht, Utrecht, the Netherlands; To investigate the effect of HIV-1 infection on the function of mononuclear phagocytes. In this study, the interaction of uninfected or HIV-1-infected U(937) cells with opsonized M. avium was compared. M. avium was directly labeled with FITC, and opsonized with normal human pooled serum (HPS, HIV-ne
A human env-like protein: possible relevance in HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:654 (abstract no. C.578); Grassi F, De Santis C, Beretta A, Gullberg M, Siccardi AG; Dipartimento di Biologia e Genetica per le Scienze Mediche, Universita di Milano, Italia; To characterize a human env-like protein. A 45 KDa human lymphocyte activation-associated protein sharing an epitope with env gp120 has been described and named HELP (human env-like protein). Two monoclonal antibodies reactive with HELP have been produced: one of them (M38) is reactive with both the
NMR study of perturbations of phosphatidylcholine bilayers by diacylglycerols.: Int Conf AIDS 1989 Jun 4-9; 5:655 (abstract no. C.581); DeBoeck H, Zidovetzki R; Department of Biology, University of California, Riverside, CA 92521, USA; Addition of diacylglycerols to the cultured HIV-infected cells was recently shown to significantly reduce cytopathological effects of the virus. We used a combination of 31p and 2H nuclear magnetic resonance (NMR) to characterize the perturbations of phosphatidylcholine bilayers by a series of saturated diacylglycerolo
Effect of neurotropin on a specific host cell membrane protein, HMP-1, induced with HIV-infection and functionally associated with suppression of giant cell formation.: Int Conf AIDS 1989 Jun 4-9; 5:655 (abstract no. C.582); Takeoka Y, Yashiki S, Maruyama I, Suehiro S, Sonoda S; Dept. of Virology and 3rd Dept. of Internal Medicine Faculty of Medicine, Kagoshima Univ., Kagoshima, Japan; The effect of Neurotropin(R), a non-protein extract from cutaneous tissue of rabbit inoculated with vaccinia virus and contains biologically active substances formed by immuno-inflammatory reaction, on the formation of giant cell in HIV-infected MT-2 cells was investigated. 10-100 mu g/ml of Neurotropin introduced to M
Nitrophenyl derivatives (NP) as potential anti-human immunodeficiency virus (HIV) agents.: Int Conf AIDS 1989 Jun 4-9; 5:655 (abstract no. C.583); Matsiota P, Guetard D; Immunocytochimie, Paris, France; To assess the in vitro anti-HIV activity of NP derivatives. METHODS & We report that nitrophenyl derivatives (NP) (dinitrophenyl-glycine, trinitrophenyl-bovine serum albumin) neutralize HIV as assessed by reverse transcriptase activity determination in culture supernatants of peripheral blood mo
Treatment of major depression, new onset, in patients with HIV positive serology without active disease with fluoxetine.: Int Conf AIDS 1989 Jun 4-9; 5:653 (abstract no. C.584); Levine SH; University of California, USA; Seven patients were referred from the UCLA AIDS Research Center who met criteria for DSMIII-R Major Depression. All of these patients were participating in an AZT double-blind placebo- controlled study at the Center. Five of the patients had positive HIV serology but no manifestations of the disease; the remaining two
Characterization of tannins containing anti-HIV activity.: Int Conf AIDS 1989 Jun 4-9; 5:656 (abstract no. C.585); Kilkuskie RE, Cheng YL, Lee KH, Bodner AJ; Biotech Research Laboratories, Inc., Rockville, MD 20850, USA; To characterize anti-HIV activity of purified tannins. Tannins were purified from commercial tannic acid by chromatography through Sephadex LH-20 and preparative high performance liquid chromatography with reverse phase columns. HIV-1 reverse transcriptase was purified by immunoaffinity chromatograp
Treatment of AIDS associated cryptosporidiosis with hyperimmune colostrum from cows vaccinated with cryptosporidium.: Int Conf AIDS 1989 Jun 4-9; 5:656 (abstract no. C.586); Nord J, Ma P, Tacket CO, Dijohn D, Tzipori S, Sahner D, Shieb G; St. Vincent's Hosp. & Med. Ctr. of New York, NY, NY, USA; To assess the efficacy of hyperimmune bovine colostrum against Cyptosporidium in symptomatic AIDS patients. Bovine colostrum was obtained from cows hyperimmunized with Cryptosporidium antigens. 5 AIDS patients with diarrhea and Cryptosporidiosis were given the colostrum by continuous infusion via fe
The effect of ozone (03) on lymphocyte populations in normal and HIV1-infected blood.: Int Conf AIDS 1989 Jun 4-9; 5:656 (abstract no. C.587); Wagner K, Mayers D, Toro L, Baker JR Jr; Henry M Jackson Foundation, Rockville, MD., USA; Measure the effect of varying 03 concentrations on lymphocytes in whole blood from an uninfected and a Walter Reed Stage 2 HIV1 patient. Heparinized whole blood samples were exposed in triplicate to (03) of 20, 40, and 60 ug/ml with an oxygen control. Coded, blinded blood samples were gently agitate
DEC and PER capita AIDS cases.: Int Conf AIDS 1989 Jun 4-9; 5:656 (abstract no. C.588); Kitchen L; Tulane University Medical Center, New Orleans, LA, USA; Administration of the antifilarial/immunomodulator drug diethylcarbamazine (DEC) frequently alters virologic, hematologic, serologic, and clinical parameters in cats infected with feline leukemia virus--an animal model for AIDS. DEC is an orally bioavailable drug, and some experiments suggest that DEC is con
Women underrepresented in AIDS clinical trials.: Int Conf AIDS 1989 Jun 4-9; 5:656 (abstract no. C.590); Long I, Kirschenbaum D, McCarthy M; AIDS Coalition to Unleash Power, New York, New York, USA; To monitor the access of women to experimental AIDS treatments in the United States . METHOD: We collected data on women enrolled in U.S. Government ACTG clinical trials to identify hindrances to health care. Current Food and Drug Administration ( FDA ) policy restricts access of all pat
In vitro evaluation of effects of LF1695 on HIV infected cells, alone or in association with antivirals.: Int Conf AIDS 1989 Jun 4-9; 5:657 (abstract no. C.591); Dubeaux D, Delestre L, Clayette P, Abita JP, Lizard G, Dormont D; Center de Recherches du Service de Sante des Armees, Commissariat a lEnergie Atomique, Fontenay-Aux-Roses, France; To determine the cellular target of an immune modulator drug, LF1695, in HIV infected PBL and infected cell lines, and to evaluate potential interactions with antivirals. Interactions of LF1695 with lymphocyte membrane have been studied by Spin Label Method and competition assays between monoclonal
Inhibition of HIV-1 and HIV-2 replication in fresh and chronically infected cells with antiviral agents.: Int Conf AIDS 1989 Jun 4-9; 5:657 (abstract no. C.592); Sun DK, Pontani D, Thornton AH, Fullen J, Shahied SI, Sarin PS; Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland USA; We have examined and compared the inhibition of HIV-1 and HIV-2 replication in fresh and chronically infected cell cultures by a number of drugs directed towards various stages in the virus life cycle. We have evaluated the effect of these drugs, including AZT , DDC, foscarnet, amphotericin methyl ester (AM
Biochemical pharmacology of AZT in human t-lymphoblastoid cells (CEM).: Int Conf AIDS 1989 Jun 4-9; 5:657 (abstract no. C.593); Vassilios LA, Wendy M, Gomperts E, Holcenberg JS; Childrens Hospital of Los Angeles CA, USA; The purpose of this study was to investigate the biochemical pharmacology of AZT and to examine the effect of AZT on nucleoside triphosphate pools in virus-free human T-origin line (CEM) cultured in vitro. Although the inhibitory concentration 50% (IC50) of AZT in this cell line averaged 3.90 +/- 2.
Differential antiretroviral activity of dextran sulfate, pentosan polysulfate and heparin against HIV-1 (HTLV-IIIB) and HIV-2 (LAV-2 ROD) in MT-4 cells.: Int Conf AIDS 1989 Jun 4-9; 5:657 (abstract no. C.595); Pauwels R, Baba M, Schols D, Balzarini J, Desmyter J, De Clercq E; Rega Institute for Medical Research, K.U. Leuven, B-3000 Leuven, Belgium; Since sulfated polysaccharides interfere with the interaction of the highly variable envelope glycoproteins of HIV with the cellular CD4 receptor, we wondered whether these compounds may have differential effects on different HIV-1 and HIV-2 strains. Anti-HIV activity was determined 5 days post-infe
GLQ223: An inhibitor of HIV replication in acutely and chronically infected t cells and macrophages.: Int Conf AIDS 1989 Jun 4-9; 5:657 (abstract no. C.596); McGrath MS, Hwang KM, Caldwell SE, Gaston I, Luk KC, Lifson JD; San Francisco General Hospital, San Francisco, California, USA; To evaluate GLQ223 for ability to inhibit HIV replication in acutely and chronically infected cells and cells of both lymphocyte and mononuclear phagocyte lineage. GLQ223 is a highly purified plant derived protein used, in crude form, in traditional herbal medicine for a variety of clinical indicati
In vitro inhibition of HIV replication by cholic acid.: Int Conf AIDS 1989 Jun 4-9; 5:658 (abstract no. C.597); Theodoropoulou M, Cornuet P, Gravanis A, Stournaras C, Psyonoyos, Jasmin C, Georgoulias V; School of Medicine, University of Crete, Greece; To determine whether the physiological constituant of the non-receptive eudometrium cholic acid (CA) which is displaying both ovotoxic and spermotoxic activity could also inhibit HIV replication. Different concentrations of CA were incubated with either the HIV reverse transcriptase (RT) enzyme or w
Suppression of HIV reservoir cells by membrane-active substances.: Int Conf AIDS 1989 Jun 4-9; 5:658 (abstract no. C.598); Willer A, Buff K, Mellert W, Goebel FD, Erfle V; GSF-Abteilung fur Molekulare Zellpathologie, 8042 Neuherberg/Munchen, FRG; HIV can productively infect several cell types such as monocytes/macrophages and lymphocytes in vivo without subsequent cytolysis, thus creating a virus reservoir . Strategies to suppress or to eliminate virus reservoir cells have been designed using the HIV-induced membrane alterations. The influen
Community-based research in AIDS: CRI as model.: Int Conf AIDS 1989 Jun 4-9; 5:658 (abstract no. C.601); Thomas H; NY Community Research Initiative; New York, NY USA; To demonstrate how community-based research can represent a successful alternative model for the design and conduct of clinical research in AIDS, expanding the number of study participants and increasing compliance (especially including minorities and groups normally underrepresented in trials), employing co
DHEA and DHEAS in AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:658 (abstract no. C.602); Sonnabend J, Fleischer T, Seaman JD; Community Research Initiative New York, NY, USA; To describe results of a study of dehydroepiandrosterone (DHEA) and DHEA-sulfate (DHEAS) in people with AIDS (PWAs). Blood samples were drawn between 2:00 and 5:00 PM from 17 PWAs; they were submitted to a commercial laboratory for radioassay determinations of DHEA and DHEAS levels as well as for de
MM-1 in the treatment of AIDS: report on 172 treated patients.: Int Conf AIDS 1989 Jun 4-9; 5:659 (abstract no. C.603); Shafik A, Lurhuma Z, Diese M, Wayne J; Cairo University, Cairo, Egypt; The result of treatment of 172 AIDS patients with MM-1, an antiviral drug, is presented. Twenty AIDS patients were treated with the standard treatment for opportunistic infections and were considered as controls. Patients were monitored for clinical and laboratory changes before, during and after treatment. MM-1 was gi
Inhibition of expression of natural UAG suppressor glutamine tRNA in HIV- and Moloney virus-infected human H9 cells in vitro by avarol.: Int Conf AIDS 1989 Jun 4-9; 5:659 (abstract no. C.606); Muller WE, Kuchino Y, Schroder HC; Institut fur Physiologische Chemie, Universitat, Duesbergweg, 6500 Mainz, West Germany; To study the mode of action of the anti-HIV compound Avarol. Human T cells (H9), infected with HIV-1 and NIH-3T3 cells, infected with Moloney Murine Leukemia Virus (Mo-MuLV) have been used for the studies. Avarol is a sesquiterpenoid hydroquinone, which causes a strong antiviral effect on H
Inhibition of HIV replication by xylan-polyhydrogensulfate: hoe/bay 946, a new antiviral compound in clinical trial.: Int Conf AIDS 1989 Jun 4-9; 5:659 (abstract no. C.607); Biesert L, Suhartono H, Winkler I, Schrinner E, Schlumberger HD, Rubsamen-Waigmann H; Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus, Frankfurt, FRG; HOE/BAY 946, a xylanpoly-(hydrogensulfate) disodium salt, inhibited HIV-1 and HIV-2 replication and virus-induced cell fusion in lymphocyte cultures at concentrations above 25 ug/ml (4.2 umol/l) totally. The drug was found to be an inhibitor of the HIV-enzyme reverse transcriptase. Experiments were performed
Hypericin mediates anti-HIV effects in vitro.: Int Conf AIDS 1989 Jun 4-9; 5:659 (abstract no. C.608); Weiner DB, Lavie G, Williams WV, Lavie D, Greene MI, Muerelo D; Department of Pathology, Department of Medicine, University of Pennsylvania, Philadelphia PA, 19104-6082, USA; To examine the ability of two aromatic polycyclic diones hypericin and pseudohypericin to control HIV infection in vitro. Evaluation of syncytia formation, reverse transcriptase activity, Viral RNA production, biochemical as well as electron micrographic studies of HIV-1 and HIV-2 have been studied.
Evaluation of erythro-9 (2-hydroxy-3-nonyl) adenine (EHNA) treatment on HIV-1 replication.: Int Conf AIDS 1989 Jun 4-9; 5:660 (abstract no. C.609); Sei Y, Tsuboi I, Yokoyama MM, Arora K; Laboratory of Neuroscience, NIDDK, National Institutes of Health, Bethesda, MD, USA; To evaluate the efficacy of EHNA treatment on viral replication in HIV-1 infected cell lines in vitro. HIV-1 infected or uninfected H9 cell lines were treated with varying concentrations of erythro-9(2-hydroxy-3-nonyl) adenine (EHNA), an inhibitor of the common Adenosine deaminase (ADA) isozyme(s) (
Controlled evaluation of the effect of zidovudine on neuropsychological test performance in CDC group IV HIV infected homosexuals.: Int Conf AIDS 1989 Jun 4-9; 5:660 (abstract no. C.610); Kocsis AE, Vearnals S, Church J, Helbert M, Peters B, Pinching A; Psychology Department and Immunology Dept., St. Mary's Hospital, London, W2 1NY, UK; To evaluate the effectiveness of Zidovudine in reversing cognitive impairment in CDC Group IV HIV infected patients and to judge the speed of drug effectiveness. METHOD: Monthly evaluation on a battery of neuropsychological tests was carried out on Zidovudine treated patients (N=20) and control groups: (1)
Anti-HIV-1 activities of sulfated monosaccharides (SMS) and polysaccharides (SPS): therapeutic indicies (TI).: Int Conf AIDS 1989 Jun 4-9; 5:660 (abstract no. C.612); Bagasra O, Lischner HW, Halligen GE, Heins B; Univ. of Medicine and Dentistry of New Jersey, Camden, NJ, USA; To compare the TI in vitro of anti-HIV SMS and SPS. SPS including dextran sulfates (DS) of various mw, heparin, heparin sulfate, chondriotin sulfate (shark and bovine) pentosan polysulfate, Fucoidin, and carrageenan as well as SMS including D-glucosamine 2 sulfate, D-glucosamine 6 sulfate, D-glucosa
Blocking of HIV-I binding site by lectins.: Int Conf AIDS 1989 Jun 4-9; 5:660 (abstract no. C.613); Bagasra O, Heins B, Whittle P, Lischner HW; University of Medicine and Dentistry of New Jersey-SOM, Camden, NJ, USA; Blockade of the interaction of HIV-1 gp120 molecule with CD4-ligands is one of the many stratagems for blocking HIV-1 infection and possibly cytopathicity. The use of purified lectins, which are specific for one or two sugars, as a blocking agent may provide a rather simple way of HIV-neutralization. METHODS
The development of new drugs to treat HIV infection: the role of government and private sector institutions.: Int Conf AIDS 1989 Jun 4-9; 5:661 (abstract no. C.617); Roland M, Schmidt RM; Center for Preventive Medicine and Health Research, San Francisco State University, San Francisco, CA, USA; To examine the role of government and the private sector in the development of new drugs to treat HIV-associated infections and cancers. We reviewed: the organization and function of the U.S. Public Health Service (PHS), funding mechanisms for PHS agencies responsible for AIDS, implementation of AID
Factors affecting participant compliance with zidovudine clinical trial PO19.: Int Conf AIDS 1989 Jun 4-9; 5:661 (abstract no. C.618); Cooke M, McKusick L; Department of Medicine, Center for AIDS Prevention Studies, University of California, San Francisco, CA, USA; To determine what factors correlate with adherence or non-adherence to protocol PO19 of zidovudine, so that recommendations can be made regarding the structure of AIDS clinical trials. 50 adherents and 50 dropouts from the San Francisco sites of clinical trial PO19 were interviewed and administered
Disulfiram as an immunomodulating agent - follow up.: Int Conf AIDS 1989 Jun 4-9; 5:661 (abstract no. C.619); Bihari B, Fleischer T, Devine J, Seaman JD; SUNY/HSCB; CRI, New York, NY, USA; To describe the results of an expanded trial of disulfiram in AIDS/ARC patients of CRI community physicians. We initially monitored 53 AIDS/ARC patients who were taking disulfiram. Results of that monitoring included 1) significant increases in CD4 cells, 2) reduction of clinical symptoms, and 3) m
Synergism and potentiation effect of a combination of different drugs, directed towards various stages of the virus life cycle, in the treatment of AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:661 (abstract no. C.620); Sarin PS, Sun DK, Pontani D, Thornton A, Fullen J, Shahied SI; Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD, USA; A number of antiviral agents that inhibit human immunodeficiency virus (HIV-1) in cell culture (MOLT-3, H9) have shown unacceptable levels of toxicity in human clinical trials. We have evaluated a number of drugs, including AZT , DDC, foscarnet and amphotericin methyl ester (AME), at levels much lower than traditionall
Anti-HIV activity of dextran sulfate as determined under different experimental conditions.: Int Conf AIDS 1989 Jun 4-9; 5:662 (abstract no. C.621); Nakashima H, Yoshida O, Baba M, De Clercq E, Yamamoto N; Department of Virology and Parasitology, Yamaguchi University School of Medicine, 1144 Kogushi, Ube, Yamaguchi 755, Japan, and Rega Institute for Medical Research, K.U. Leuven, 13-3000 Leuven, Belgium; Dextran sulfate is a potent and selective inhibitor of HIV-1. Its anti-HIV-1 activity has been investigated under varying experimental conditions. MT-4 cells were infected with HIV-1 at different multiplicities of infection (m.o.i.), and treated with either dextran sulfate, 3 -azido-2 ,3 -dideoxythymidine (
An HIV-1 p24 Screening assay for detection of HIV-1 Inhibitors.: Int Conf AIDS 1989 Jun 4-9; 5:662 (abstract no. C.622); Corrigan AJ, Kilkuskie RE, Kaushal IA, Adams-Mackie BA, Youmans MG, Bodner AJ; Biotech Research Laboratories, Inc., Rockville, MD 20850, USA; To validate a sensitive, rapid, and reproducible screening assay for HIV-1 inhibitors. H9 lymphocytes are treated for 1 hour at 37 degrees C with an aliquot of a frozen stock of HIV-1 virions at a multiplicity of infection of 0.3 TCID50/cell. The infected cultures are washed 3 times to remove unadso
In vitro efficacy of the polysulfated polyxylan HOE/BAY 946 in HIV infected human monocytes/macrophages.: Int Conf AIDS 1989 Jun 4-9; 5:662 (abstract no. C.623); von Briesen H, Meichsner C, Andreesen R, Rubsamen-Waigmann H, Schlumberger HD, Schrinner E; Georg-Speyer-Haus, Frankfurt, FRG; To present data demonstrating the efficacy of xylan(-hydrogen-sulphate) disodium salt (HOE/BAY 946) in HIV infection of human monocytes/macrophages. A system has been developed in which human monocytes/macrophages from the blood of healthy donors are cultured on hydrophobic membranes. Cells which ha
Inhibition of HIV reverse transcriptase by 4-quinolones.: Int Conf AIDS 1989 Jun 4-9; 5:662 (abstract no. C.624); Gurtler L, Eberle J, Deinhardt F; Max von Pettenkofer Institute, University of Munchen, FRG; One way to decrease the HIV load in symptomatic patients is to reduce virus replication. We looked for chemical compounds to achieve this. MATERIAL AND Ofloxacin, ciprofloxacin and norfloxacin were used at concentrations of 1 to 5 muM in 0.155 M sodium chloride, 0.01 M glycine-lactate buffer, pH 4.0
Energy calculations on anti-HIV nucleosides: enhanced stability of C31-exo twist conformations in active compounds.: Int Conf AIDS 1989 Jun 4-9; 5:662 (abstract no. C.625); Taylor EW, Thompson BB, Van Roey P, Chu CK; College of Pharmacy, The University of Georgia, Athens, GA 30602 USA; We recently reported (Taylor et al., 1989 ACS Abstr., Dallas) that for AZT and 2 other azide analogs, low energy conformers predicted by molecular mechanics (MM) correlate well with the observed crystal structures. The preferred conformations for the furanose ring in these azide analogs are C3 -exo (3T2 and 3T4); this
Mechanisms of the anti-retroviral activity of aromatic polycyclic diones hypericin and pseudohypericin.: Int Conf AIDS 1989 Jun 4-9; 5:662 (abstract no. C.626); Meruelo D, Lavie G; Dept. of Pathology, New York University School of Medicine, New York, NY USA; We have recently reported that the polycyclic aromatic dione compounds hypericin (HY) and pseudohypericin (PS) inhibit the replication and infection cycles of a variety of murine retroviruses. These studies have now been extended to include the human HIV. If a murine leukemia cell line (AQR) infected with and producing
Baculovirus directed cell-surface expression and purification of CD4 in insect cells.: Int Conf AIDS 1989 Jun 4-9; 5:663 (abstract no. C.627); Webb NR, Madoulet C, Tosi PF, Broussard DR, Sneed L, Summers MD, Nicolau C; Texas A&M University, College Station, Texas, USA; Infection by the human immunodeficiency virus (HIV), the causative agent of acquired immune deficiency syndrome (AIDS) is mediated by the binding of the viral envelope glycoprotein, gp120, to CD4, a glycoprotein expressed on the surface of HIV-susceptible cells, most notably T helper lymphocytes (Sattentau and Weiss, 1
[Double blinded controlled trials of LF 1695 in HIV+ patients]: Int Conf AIDS 1989 Jun 4-9; 5:663 (abstract no. C.628); Livrozet JM, Crimet D, Kienzler JL, Touraine F, Touraine JL; Pavillon P, Hopital E. Herriot Lyon, France
In vitro anti-HIV activity of a new class of polymers.: Int Conf AIDS 1989 Jun 4-9; 5:663 (abstract no. C.630); Lemaitre M, Boyer B, Roque JP, Lamaty G, Montagnier L, Zerial A; Rhone-Poulenc SANTE, Research Center, F-94403 Vitry-Cedex, France; We have synthesized a new class of polymers and tested their ability to act as HIV inhibitors. We have tested these compounds as HIV replication inhibitors in CEM clone 13 cells and in human peripheral blood lymphocytes (PBL). We have measured the reduction of cytopathic effect (CPE) induced by HIV-
Cellular pharmacology of 3'azido-2'-3'-dideoxy-uridine (AzddU) in human primary cells: evidence of a novel metabolite.: Int Conf AIDS 1989 Jun 4-9; 5:663 (abstract no. C.631); Zhou Z, Schinazi RF, Eriksson BF, Chu CK, Williams G, Sommadossi JP; Univ. of Alabama at Birmingham, Birmingham, AL USA; To investigate the cellular pharmacology of AzddU (CS-87) and 3 -azido-3 -deoxythymidine ( AZT ) in human bone marrow (BM) and peripheral blood mononuclear (PBM) cells. Human PHA-stimulated PBM or BM cells (2 X 10(6) cells/ml) were exposed to 10muMU(3H) AZT or (3H) AzddU from 2 to 48 hrs.
Immunostimulatory effects of beta-carotene on T-cell activation markers and NK cells in HIV infected patients.: Int Conf AIDS 1989 Jun 4-9; 5:663 (abstract no. C.632); Watson RR, Garewal HS, Ampel NM, Prabhala RH, Allen V, Dols C, Hicks MJ; University of Arizona, Tucson, Arizona, USA; Study the effect of beta-carotene on lymphocytes from patients with AIDS, based on its immunostimulatory effects in normal subjects. Eleven HIV infected patients were given 30 mg/day of beta carotene for 4 months. Samples of heparinized peripheral blood were collected prior to, during, and after ces
Reduction of p24 antigen in CDC II/III cases with BD zidovudine and progression to AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:664 (abstract no. C.633); Tang AL, Gill S, Thin RN, Bateman NT, Cordery M, Barlow D; St. Thomas' Hospital, England; To study the effect of 1200 and 800 mg Zidovudine given in 6 hrly or 12 hrly dosage on p24 antigenaemia in CDC II/ III) patients. 36 asymptomatic patients (CDC II/III) with p24 antigenaemia were randomised to receive either Zidovudine or Placebo on a double-blind basis. The results of the first 18 a
Pulmonary function in individuals with AIDS receiving aerosolized pentamidine (AP) for Pneumocystis carinii pneumonia (PCP) prophylaxis.: Int Conf AIDS 1989 Jun 4-9; 5:664 (abstract no. C.634); Sonnabend J, Fleischer T, Holzemer S, Blau A; Community Research Initiative, New York, USA; To monitor pulmonary function in 205 patients with AIDS enrolled in a 1-year trial of two doses of AP for prophylaxis of PCP , which was fully enrolled by 6/28/88. 75.4% of study participants had experienced a prior episode of PCP. Pulmonary function tests (PFTs) including measurements of DLCO, FEV,
Dextran sulfate decreases cell surface GP120 but does not interfere with GP120-CD4 interactions.: Int Conf AIDS 1989 Jun 4-9; 5:664 (abstract no. C.635); Callahan L, Phelan M, Norcross MA; Food and Drug Administration, Bethesda, MD. 20892 USA; Dextran sulfate has been reported to block virus binding to CD4+ cells. To determine if this effect is due to receptor blockage, we examined the effect of dextran sulfate on gp120 to CD4 binding and cell surface gp120 expression. Gp120 binding to CD4 was measured by incubating recombinant gp120 (
Effect of amphotericin B and its methyl ester (AME) on human immunodeficiency virus infection and phagocytosis in macrophage cultures.: Int Conf AIDS 1989 Jun 4-9; 5:664 (abstract no. C.636); Pontani DR, Sun DK, Wolf B, Bryant P, Shahied SI, Brown J, Rosen J, Plescia OJ, Schaffner CP, Sarin PS; USA; The polyene antibiotic amphotericin B and its methylated derivative AME were tested in the continuous macrophage cell line U937 for activity against HIV-1. At non-cytotoxic dose levels the expression of HIV-1 was inhibited as shown by the measurement of p24 antigen expression with immunofluorescence. At 10 ug/ml there
Effect of the polyene macrolide group of antibiotics on inhibition of Human Immunodeficiency Virus (HIV) replication in cell culture.: Int Conf AIDS 1989 Jun 4-9; 5:664 (abstract no. C.637); Pontani DR, Shahied SI, Zuberbuehler D, Swiatek G, Sun DK, Sarin PS; New Jersey State Department of Health, Trenton, New Jersey USA; The polyene macrolide family of antibiotics contain several potent antifungal compounds and act by binding to a sterol moiety in the membrane of cells. They have recently been shown to be effective inhibitors of HIV in vitro due to the presence of high cholesterol-phospholipid ratio in this virus. These antibiotics are
Mechanistic assays for natural product anti-HIV activity.: Int Conf AIDS 1989 Jun 4-9; 5:664 (abstract no. C.638); McMahon J, Weislow O, Kiser R, Gulakowski R, Warren J, Gustafson K, Bader J, Boyd M; Developmental Therapeutics Program, DCT, NCI, Frederick, MD, USA; To develop rapid quantitative microassays to study the mechanisms of action of compounds with anti-HIV activity isolated from natural products. Microculture procedures were utilized to define the various stages of HIV infection in T-lymphoblastoid cells. Quantitative readouts of these assays include
A trial of aerosolized pentamidine (AP) for prophylaxis of Pneumocystis carinii pneumonia (PCP) in AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:665 (abstract no. C.639); Sonnabend J, Holzemer S, Fleischer T; Community Research Initiative, New York, USA; To investigate the safety of 100 and 150mg of AP delivered biweekly to individuals with AIDS, as well as the efficacy of these dosages in the prevention of PCP . Eligible participants who had CDC-defined AIDS, were randomized to receive either 100 or 150mg of AP biweekly using the Marquest Respigard
A critique of the US. government AIDS clinical trial program.: Int Conf AIDS 1989 Jun 4-9; 5:665 (abstract no. C.640); Long I, Kirschenbaum D, Huff R; AIDS Coalition to Unleash Power, New York, New York, USA; To evaluate accessibility of experimental treatments in the AIDS Clinical Trial Group (ACTG) Program to HIV-infected people. In January 1989, this program had enrolled over 5,700 subjects in trials at 35 sites and 11 pediatric centers. Government documents related to these trials were reviewed perio
Specific interaction of aurintricarboxylic acid with the HIV/CD4 receptor.: Int Conf AIDS 1989 Jun 4-9; 5:665 (abstract no. C.641); Schols D, Baba M, Pauwels R, Desmyter J, De Clercq E; Rega Institute for Medical Research, K.U. Leuven, B-3000 Leuven, Belgium; Considering the essential role of the CD4 receptor in the replicative cycle of HIV, it would seem important to find appropriate marker molecules that specifically interact with the CD4 receptor and thereby block HIV attachment to the cells. CD4+ cells (MT4, U-937, peripheral blood lymphocytes and monocytes) were incuba
[Variability of viral sequences in AIDS]: Int Conf AIDS 1989 Jun 4-9; 5:665 (abstract no. C.643); Chappey C, Sonigo P, Hazout S, Valleron AJ; INSERM U263, INSERM U163, Institut Pasteur, Paris, France
Difference in long terminal repeat activity of two human immunodeficiency virus-1 variants from the same patient.: Int Conf AIDS 1989 Jun 4-9; 5:666 (abstract no. C.645); Golub E, Li G, Volsky DJ; Molecular Virology Lab, St. Luke's/Roosevelt Hosp. Ctr. and College of Physicians and Surgeons, Columbia University, New York, NY, USA; To compare transcriptional activity of long terminal repeats (LTR) of different HIV-1 variants. LTR activity was analyzed by transient chloramphenicol acetyltransferase expression assay or by measuring production of p24 protein. Two HIV-1 variants, derived from a single parental isolate and
Delayed & slow expression of HIV-1 RNA & tat protein during infection with a non-cytopathic HIV-1 virus.: Int Conf AIDS 1989 Jun 4-9; 5:666 (abstract no. C.646); Sakai K, Ma XY, Sinangil F, Volsky DJ; St. Luke's/Roosevelt Hospital Center and College of Physicians and Surgeons, Columbia University, New York City, NY, USA; To study the mechanism of infection with a non-cytopathic isolate of HIV-1. The non-cytopathic and cytopathic HIV-1 isolates NIT-E and NIT-A, respectively, were obtained from the virus-producer CEM/HIV-1/N1T cell line by molecular cloning. Virus entry was measured as a function of the virus-cell fus
Expression of gag proteins of human immunodeficiency virus using recombinant adenovirus vectors.: Int Conf AIDS 1989 Jun 4-9; 5:666 (abstract no. C.647); Molnar-Kimber KL, Mastroeni R, Dheer SK, Natuk R, Bhat B, Chanda P, Mizutani S, Davis AR, Hung PP; Biotechnology and Microbiology Division, Wyeth-Ayerst Research, P.O. Box 8299, Philadelphia, PA USA 19101-1245; To develop Ad4-HIV recombinant adenovirus type 4 vaccine candidates, the gag-pol gene of Human Immunodeficiency Virus was inserted into the Ad4 E3 region. In attempts to generate a new late transcription family, an oligomer which contained 32 bp of the splice acceptor from the L5 transcription family was inserted upstr
Purification of recombinant HIV proteins by iso-electric focusing.: Int Conf AIDS 1989 Jun 4-9; 5:666 (abstract no. C.648); Chen A, Winslow E, Stafford A, Sass J, Siaw M, Van Winkle M; Beckman Instruments, Inc., Irvine, CA 92714; A preparative isoelectric focusing (IEF) method using a commercially available apparatus was applied to the purification of recombinant HIV proteins expressed in E. coli. Proteins representing env, gag and pol domains were extracted from inclusion bodies and initially purified through varying sequences of ion-exchange
Construction of HIV-1 inter-isolate recombinants.: Int Conf AIDS 1989 Jun 4-9; 5:666 (abstract no. C.649); York D, Borissenko L, Bauer D, Cheng-Mayer C, Levy J, Dina D; Chiron Corporation, Emeryville, CA, USA; Construction of inter-isolate recombinant HIV-1 viruses using molecularly cloned DNA from viruses with distinct biological properties was undertaken to identify the genes determining those specific biological properties. The HIV-1 isolates SF-2 and SF-33 differ biologically in their ability to form
No primary detection of HIV amplified DNA sequences in urine sediments of seropositive individuals.: Int Conf AIDS 1989 Jun 4-9; 5:666 (abstract no. C.650); Gasparini P, Mezzelani P, Savoia A, Pignatti PF, Morandi C, Venturini L, De Sandre G; Institute of Biological Sciences - University of Verona - Italy; To validate the data on urine infectivity in seropositive individuals, searching HIV DNA by polymerase chain reaction (PCR) technique in their urine sediments. We collected 50 ml urine and 20 ml peripheral blood from five HIV seropositive drug addicts. The cell pellet was resuspended in 500 ul water
HIV/STD-contaminated human sperm motility; diagnosis and "experimental isolation of sperm using "swim up" technique in Wang's tube and computerized image analysis.: Int Conf AIDS 1989 Jun 4-9; 5:667 (abstract no. C.651); Wang FN, Cheng CM, Hafez ES; Microbofre Scientific Institute, Republic of China; The human semen contains high titers of HIV/STD s. Little is known about the mechanisms of the life cycle of HIV in semen heterosexual transmission and its role in HIV particles, released from infected lymphocytes in the semen, blinds randomly is seminal plasma in HIV, attached to specific domains on the surface membra
Molecular andrology and HIV heterosexual transmission: an overview.: Int Conf AIDS 1989 Jun 4-9; 5:667 (abstract no. C.652); Hafez ES; World Federation of Contraceptive Health; Reproductive Health Center, Surfsong Road, Kiawah, SC USA; HIV reservoirs in seropositive people seem to include: a) the epithelium of testis/epidermis, colorectum; b) monocytes/ macrophages/endothelial cells of the uterine cervix; c) macrophages, antibody-producing B cells, endothelial cells of blood vessels, neuronal and non-neuronal brain cells. There are remarkable variat
Role of HIV envelope gene in the pathogenesis of HIV.: Int Conf AIDS 1989 Jun 4-9; 5:667 (abstract no. C.653); Tudor G, Hoxie J, Inoue M, Reddy EP; Wistar Institute and The University of Pennsylvania 19104 USA; The role of HIV envelope gene in the pathogenesis of HIV was studied following two approaches. First portions of the envelope gene were mutagenized and their effect on the biological properties of HIV analyzed following the rescue of the virus from transfected cells. In a second approach, naturally occurring mutants of
Alteration of (2'-5') oligoadenylate metabolism and ribonuclease L activity in H9 cells by HIV.: Int Conf AIDS 1989 Jun 4-9; 5:667 (abstract no. C.654); Muller WE, Schroder HC; Institute fur Physiologische Chemie, Universitat, Duesbergweg, 6500 Mainz, West Germany; To study the alteration of the 2 ,5 -oligoadenylate less than (2 -5 ) A greater than metabolism in HIV-1-infected cells in relation to HIV production. Human T cells (H9), infected with HIV-1, have been used for the studies. The synthesis of (2 -5 ) A was found to proceed in close associatio
Diffuse infiltrative CD8 lymphocytosis in HIV infection is associated with a particular subtype of HLA-DR5.: Int Conf AIDS 1989 Jun 4-9; 5:667 (abstract no. C.655); Itescu S, Brancato L, Gregersen PK, Winchester R; Hospital for Joint Diseases, NYU Medical Center, New York, NY, USA; A disorder characterized by circulating and diffuse infiltrative CD8 lymphocytosis (DILS), predominantly involving the lungs and salivary glands, has been associated with HLA-DR5 in HIV-infected individuals. We sought to determine the relative risk conferred by HLA-DR5 to developing DILS, and to define the p
Synthesis, processing and secretion of an immunodominant epitope of the GP41 of HIV expressed in yeast.: Int Conf AIDS 1989 Jun 4-9; 5:667 (abstract no. C.656); Gairin JE, Madaule P, Rossier J; Laboratoire de Physiologie Nerveuse, CNRS, 91198 Gif/Yvette Cedex, France; AIM: We describe a very efficient method for the production of a glycosylated peptide which is an immunodominant epitope in the gp41 of HIV. METHOD: We used a new expression vector pSE-X which allows the synthesis and secretion of foreign gene products in S. Cerevisiae. The foreign DNA is inserted downstream the leader
A rapid and sensitive microassay to detect HIV-LTR activity in human cell lines using luciferase luminescence.: Int Conf AIDS 1989 Jun 4-9; 5:668 (abstract no. C.658); Hazan U, Schwartz O, Henin Y, Montagnier L, Virelizier JL; Unite d'onclogie virale, Laboratoire d'immunolgie virale, Inst. Pasteur, Paris, France; To develop a rapid, sensitive and unexpensive microassay that allows study of transactivator or inhibitor factor effects on HIV promoter. Plasmids were constructed with the reporter gene firefly luciferase under the control of different versions of HIV-LTR. These plasmids were transfected in differe
Sequence variation within and between HIV-infected patients.: Int Conf AIDS 1989 Jun 4-9; 5:668 (abstract no. C.659); Balfe P, Simmonds P, Leigh Brown AJ, Bishop JO; Department of Genetics, University of Edinburgh, United Kingdom; In early 1984 33 haemophiliac patients were treated with a batch of a factor VIII subsequently shown to have been contaminated with HIV-1; 18 of these patients have since seroconverted. There is good evidence that these patients were infected only from this source. These patients form a useful set for the quantitation
HIV2 identification through PCR.: Int Conf AIDS 1989 Jun 4-9; 5:668 (abstract no. C.660); Courgnaud V, Laure F, Dazza MC, Sonigo P, Brun-Vezinet F, Brechot C; INSERM U.75 CHU Necker, Paris, France; 1) To detect HIV2 DNA sequences in uncultured PBL from infected subjects. 2) To analyze the genetic variability of different HIV2 isolates. Patients. 12 subjects with serological results clearly consistent with HIV2 infection. For 10/12 both PBL and cell cultures and for 2/12 only cell culture was available.
Automatized fluorometric screening assay for HIV-1 aspartyl protease inhibitors.: Int Conf AIDS 1989 Jun 4-9; 5:668 (abstract no. C.662); Hirel PH, Boiziau J, Parker F, Outerovitch D, Mayaux JF, Cartwright T; Institut des Biotechnologies. Rhone-Poulenc Sante Vitry, France; To devise a fluorometric assay of HIV protease which allows automated random screening of inhibitors. HIV-1 aspartyl protease was expressed to high levels in E. coli using a T7 expression system (Rosenberg et al. 1987). The fluorometric assay was based on an analogous renin assay (Murakami et al. 19
Epitope mapping of the human immunodeficiency virus gag gene products p24 and p26 with murine monoclonal antibodies.: Int Conf AIDS 1989 Jun 4-9; 5:669 (abstract no. C.663); Butman B, Venetta T, Crush-Stanton S, Swerdlow B, Youngbar P, Lockwood D; Bionetics Research Institute, Rockville, MD, USA; To map immunogenic domains of HIV-1 p24 and HIV-2 p26 using murine monoclonal antibodies and to define epitopes conserved between the two proteins. Overlapping synthetic and recombinant peptides of p24 were prepared and tested for reactivity with each monoclonal antibody (Mab) using ELISA and Wester
HIV infection of the gastrointestinal tract: comparison of the sensitivities of hybridization and immunoassay.: Int Conf AIDS 1989 Jun 4-9; 5:669 (abstract no. C.664); Kotler DP, Reka S, Cronin W, Clayton F, Fox CH; St. Luke's-Roosevelt Hospital Center, New York, NY, USA; To use hybridization and immunoassay for detecting evidence of HIV in rectal mucosa; to compare the results with histopathologic features in the tissues. Rectal biopsies from 25 HIV seropositive subjects (19 AIDS, 6 ARC) were examined by RNA in situ hybridization, polymerase chain reaction (PCR), a
Enzyme immunoassay of recombinant HIV proteins.: Int Conf AIDS 1989 Jun 4-9; 5:667 (abstract no. C.665); Lewis S, Hufnagle G, Scott M, Bullard W, Van Winkle M; (Beckman Instruments, Inc., Irvine, CA, USA); Three enzyme immunoassays were developed to specifically quantitate 6 recombinant Type 1 HIV proteins in support of our development of ASQ-HIV assay for antibody to HIV. - Using a direct solid phase sandwich technique, antigen under study was precoated in microtiter plate wells and reacted with specific mouse monoclona
Proteolytic cleavage of the 66-Kd form of the HIV-1 reverse transcriptase.: Int Conf AIDS 1989 Jun 4-9; 5:669 (abstract no. C.666); Ferris A, Hizi A, Pichuantes S, Babe L, Craik C, Showalter SD, Hughes SH; BRI-Basic Research Program, Frederick, Maryland, USA; We wished to test the ability of viral and non-viral proteases to convert purified 66-Kd reverse transcriptase (RT) to the 51-Kd form. The 66-Kd form of HIV-1 RT and the viral protease were separately purified to homogeneity from two recombinant strains of E. coli. Proteolytic cleavage sites on HIV-
Studies on the mechanism of enhancement of HIV-envelope (env) expression by HIV-rev gene using adenoviral expression vector system.: Int Conf AIDS 1989 Jun 4-9; 5:669 (abstract no. C.667); Bhat B, Chanda P, Natuk R, Cholodofsky S, Mason B, Morin J, Dheer S, Molnar-Kimber K, Lubeck M, Mizutani S, et al; Biotechnology and Microbiology Division, Wyeth-Ayerst Research, P.O. Box 8299, Philadelphia, PA, USA; The enhanced expression (30-70 fold) of HIV-env gene in human cell line, by co-infection of Adenovirus recombinants (Ad7-env and Ad7-rev) is a good vector system to explore the direct mechanism mediated by HIV-rev gene, since no other HIV-proviral gene(s) are present in these vectors. By comparing the steady state leve
High level expression of the envelope glycoproteins of the human immunodeficiency virus (HIV) using recombinant adenovirus vectors.: Int Conf AIDS 1989 Jun 4-9; 5:669 (abstract no. C.668); Chanda PK, Natuk RJ, Greenberg L, Mason BB, Bhat B, Dheer SK, Morin JE, Molnar-Kimber KL, Lubeck MD, Mizutani S, et al; Biotechnology and Microbiology Division, Wyeth-Ayerst Research, P.O. Box 8299, Philadelphia, PA, USA; Recombinant adenoviruses containing either the envelope (env) or the rev (art/trs) genes of the human immunodeficiency virus type 1 (HIV) were constructed by inserting the genes into a cassette which contained adenovirus major late promoter, tripartite leader containing intervening sequence and the polyA. The cassette
HPLC purification of p24 antigen from HIV-1 and HIV-2 lysates.: Int Conf AIDS 1989 Jun 4-9; 5:670 (abstract no. C.669); Tyson RL, Lile CC, Kilduff J, Huskins KR, Furness JN, Reagan KJ; E. I. Du Pont Co., Medical Products Department, Glasgow Site, Wilmington, DE 19898, USA; To develop HPLC methods for the preparative isolation of the major internal core protein, p24, from HIV-1 and HIV-2 viral lysates. Doubly inactivated, gradient purified HIV-1 lysate was chromatographed on a weak anion- exchange HPLC column. Fractions were analyzed by a combination of Western blot, S
Measurement of HIV gene function in normal human monocytes/macrophages by transection.: Int Conf AIDS 1989 Jun 4-9; 5:670 (abstract no. C.670); Elbeik T, Kirihara J, Crowe S, McGrath M, Mills J; University of California, San Francisco, California, USA; To understand the function of individual HIV genes in normal human peripheral monocytes/macrophages. Both adherent and non-adherent macrophages as well as human foreskin fibroblasts were transfected with the constitutive CAT (chloramphenicol acetyl transferase) expression vector RSV-CAT and HIV LTR-
Expression, purification and immunoreactivity of pr17gag in E. coli.: Int Conf AIDS 1989 Jun 4-9; 5:670 (abstract no. C.671); McCabe D, Tribe D, Gutshall L, Devash Y, Reagan K, Erickson-Viitanen S; E.I. Dupont de Nemours and Co., Inc., Medical Products Dept. Wilmington, Delaware, USA; Plasmid pGag17, which specifies the expression in E. coli of the mature, unfused pr17gag protein was constructed by extending the gag coding sequence from the Pvu II site at nucleotide 692 of Ratner et al. (Nature, 316, 277, 1985) with a synthetic oligonucleotide linker which introduces a stop codon after the TYR codon
Complete sequence of an HIV-1 isolate determined from cDNA clones of viral RNA.: Int Conf AIDS 1989 Jun 4-9; 5:670 (abstract no. C.672); Haesevelde MV, Vanderborght B, Decourt JL, Saman E, De Leys RJ, Van Heuverswyn H; Innogenetics N.V. Antwerpen, Belgium; Oligo dT primed cDNA cloning of HIV viral RNA, allows fast analysis of virus variation. Supernantant virus from a continuous HIV-1 producing Molt 4 No. 8 cell line was pelleted and the precipitate was dissolved in GuHC1 solution and centrifuged through a CsC1 cushion. The resulting RNA pellet was fu
Chronic activation of the inositol polyphosphate pathway in HIV infected H9 lymphoblastoid cells.: Int Conf AIDS 1989 Jun 4-9; 5:670 (abstract no. C.673); Nye KE, Pinching AJ; St. Mary's Hospital Medical School, London, UK; To examine the metabolism of inositol polyphosphates, together with associated calcium changes, in H9 lymphoblastoid cells chronically infected with Human Immunodeficiency Virus (HIV) following stimulation, in order to determine whether any changes found may explain the functional defects seen in these cells
Has the major envelope glycoprotein of HIV-1 a lectin-like activity?: Int Conf AIDS 1989 Jun 4-9; 5:670 (abstract no. C.674); Gattegno L, Clerget-Rasslain B, Sadeghi H, Fenouillet E, Gluckman JC, Bahraoui E; Laboratoire de Biologie Cellulaire, Faculte de Medecine Paris Nord France; To determine whether gp 120/160 expresses carbohydrate binding properties and thus behaves as a lectin. The effects of various carbohydrates on the binding of HIV-1 or of gp 120/160 to CD4+ CEM cells was investigated by indirect immunofluorescence with gp 120-specific mouse mAbs or purified human lg
Selection of primers of maximum homology for the detection of human immunodeficiency virus sequences using the polymerase chain reaction.: Int Conf AIDS 1989 Jun 4-9; 5:671 (abstract no. C.675); Campione-Piccardo J, Arella M, Brousseau R; Molecular Virology Laboratory, Laboratory Center for Disease Control Ottawa, Ontario, Canada; Most of the oligonucleotide primers which have been proposed for the detection of human immunodeficiency virus (HIV) sequences using the polymerase chain reaction (PCR) contain mismatched bases for several HIV strains. This is particularly true for African HIV-1 and HIV-2 strains. Depending on the prevalence of the mis
Use of a cassette expression vector for the expression of human immunodeficiency virus type 1 gp160 in mammalian cells.: Int Conf AIDS 1989 Jun 4-9; 5:671 (abstract no. C.676); Salas T, Arella M, Mandy F, O'Shaugnessy MV, Campione-Piccardo J; Bureau of Laboratories and Research Services, Federal Center for AIDS, Health Protection Branch, Ottawa, Ontario, Canada; The surface glycoprotein of the human immunodeficiency virus (HIV-1) has already been expressed in vitro using different expression vectors. We have chosen the pORFEX cassette vector expression system which contains a powerful cytomegalovirus ( CMV ) promoter to express the entire gp160 in mammalian cells.
Synthesis and processing of HIV mRNA in the presence of antiviral agents.: Int Conf AIDS 1989 Jun 4-9; 5:671 (abstract no. C.677); Chatis PA, Crumpacker CS; Beth Israel Hospital/Harvard Medical School, Boston, MA, USA; Several antiviral agents inhibit the replication of HIV in vitro. Ribavirin (RBV) has been shown to interfere with post-transcriptional processing in vaccinia. We examined effects of RBV in combination with other antiviral agents on the size and amount of RNA synthesized in infected cells. Cytoplasm
A mouse monoclonal antibody against the rev protein of HIV-1.: Int Conf AIDS 1989 Jun 4-9; 5:671 (abstract no. C.679); Voll R, Aepinus C, Krapf F, Fleckenstein B, Kalden JR; Institut fur Klinische und Molekulare Virologie, Universitat Erlangen-Nurnberg, D-8520 Erlangen, FRG; The rev-protein of immunodeficiency viruses has an important function in structural gene expression. A beta-gal-rev fusion- protein containing the aminoterminal part of beta-galactosidase was synthesized in E. coli. It was used to raise a rev specific immune response in mice. After fusion with the SP2/0 cell line stabl
Characterization of an HIV-1 point mutant blocked in envelope glycoprotein cleavage.: Int Conf AIDS 1989 Jun 4-9; 5:671 (abstract no. C.680); Guo HG, Veronese F, Tschachler E, Pal R, Gallo RC, Reitz MS Jr; National Cancer Institute, Bethesda, Maryland, USA; The envelope proteins of retroviruses are derived from a polypeptide precursor protein by cleavage adjacent to a cluster of basic amino acids. Site specific mutagenesis was used to construct a mutant of the human immunodeficiency virus type 1 (HIV-1) in which the arginine residue at the carboxy terminus of the gp120 wa
Comparison of reverse transcriptase of HIV-1, HIV-2 and SIV.: Int Conf AIDS 1989 Jun 4-9; 5:672 (abstract no. C.681); Lori F, Achilli G, Cattaneo E, Sarin PS, Bertazzoni U; IGBE/CNR, Pavia, Italy; To compare the structural and biochemical properties of reverse transcriptase of HIV-1, HIV-2 and SIV. An activity gel method was used allowing identification of the active components of reverse transcriptase on polyacrylamide gel, after electrophoresis under denaturing conditions. We have
Biophysical characterization of reverse transcriptase (RT) from HIV.: Int Conf AIDS 1989 Jun 4-9; 5:672 (abstract no. C.682); Schultze P, Filipowsky ME, Kopka ML, Dickerson RE; Molecular Biology Institute, UCLA, Los Angeles, CA 90024, USA; In order to find conditions for the growth of single crystals suitable for x-ray structure analysis, various experimental techniques were applied to characterize the properties of RT in solution, especially with regard to dimerization equilibria. METHODS and Protein preparations from E. coli culture
High level expression of HIV protease in E. coli.: Int Conf AIDS 1989 Jun 4-9; 5:672 (abstract no. C.683); Olsen MK, Rockenbach SK, Tomasselli A, Tomich CS; The Upjohn Company, Kalamazoo, Michigan 49007, USA; We have obtained high level expression of the HIV protease in E. coli using the trp promoter and a runaway vector. Upon induction of the runaway function to increase plasmid copy number, the protease production results in the accumulation of inclusion bodies. In a pBR322-derived vector, the expression level is greatly
A gene family with HIV-like potential regulatory motifs.: Int Conf AIDS 1989 Jun 4-9; 5:672 (abstract no. C.684); Forsdyke D, Blum S, Sideris DP, Forsdyke RE; Department of Biochemistry, Queen's University, Kingston, Ontario, Canada K7L 3N6; To understand the molecular basis by which HIV establishes, maintains and escapes from, latency. To identify genes in T-lymphocytes which regulate, are regulated by, or are corregulated with HIV. Latent peripheral blood lymphocytes in the GO phase of the cell cycle were activated by culturing with
Needle/syringe exchange scheme based on community pharmacies, Trondheim, Norway.: Int Conf AIDS 1989 Jun 4-9; 5:672 (abstract no. C.686); Blystad H, Skjaervold K, Evjen L, Hustad T; Trondheim Health Board, Norway; To evaluate exchange scheme based on community pharmacies and local Aids clinic during 10 months trial period. Injection equipment could otherwise be purchased by ordinary sales from all pharmacies. All syringes exchanged or sold carries a label stressing the importance of sterile equipment and use
High level expression in E. coli of a truncated CD4 and its purification, refolding and activity.: Int Conf AIDS 1989 Jun 4-9; 5:673 (abstract no. C.687); Garlick RL, Kirschner RJ, Tarpley WG, Tomich CS; The Upjohn Company, Kalamazoo, Michigan 49007, USA; To develop a CD4 molecule as a potential therapeutic agent for the treatment of AIDS, we have produced a truncated sCD4 containing the N-terminal 183 amino acid residues of CD4 preceded by a methionine. The expression of this CD4-183 is in E. coli from a pBR322-based vector under the control of a trp promoter for trans
Immunoreactivity of a recombinant polypeptide consisting of the amino half of HTLV-1 p21E.: Int Conf AIDS 1989 Jun 4-9; 5:673 (abstract no. C.690); Thorn RM, Braman V, Wilde A, Seals J, Hung CH, Beltz GA; Cambridge BioScience, Worcester, MA., USA; To determine the immunoreactivity of recombinant polypeptide consisting of the amino half of HTLV-1 p21E. A DNA clone was derived that codes for the amino- terminal 132 amino acids of the HTLV-1 p21E protein (307-313 and 331-420). The clone was expressed in E. coli using a vector containing the bact
Cloning and expression of HIV-1 and HIV-2 proteases in E coli.: Int Conf AIDS 1989 Jun 4-9; 5:674 (abstract no. C.694); Simmer R, Helfrich R, Rittenhouse J, Albrecht K, Hochlowski J, Rupprecht KR; Pharmaceutical Products Division Abbott Park, Illinois., USA; To produce active recombinant HIV proteases for the rational design of specific HIV inhibitors. A gene representing the region of the HIV-1 pol gene up to the start of reverse transcriptase was constructed using seven synthetic oligonucleotides. This region codes for the 11kDa viral protease and an
Characterization of a molecular SIVagm clone.: Int Conf AIDS 1989 Jun 4-9; 5:674 (abstract no. C.696); Cichutek K, Baier M, Werner A, Kraus G, Kurth R; Paul-Ehrlich-Institut, Frankfurt am Main, FRG; SIVagm is a lentivirus closely related morphologically and genetically to HIV. 40% of African Green Monkeys (AGM) in the wild are found to be seropositive without any signs of disease. We have isolated SIVagm from several naturally infected AGMs harbored in our colony at the Paul-Ehrlich-Institut, FRG. Molecular clonin
A CD4-immunoglobulin fusion protein as a candidate for AIDS therapy.: Int Conf AIDS 1989 Jun 4-9; 5:674 (abstract no. C.697); Zettlmeissl GW, Gregersen JP, Duport JM, Seed B; Research Laboratories of Behringwerke AG, Marburg, FRG; An new antibody-like molecule (CD4H23) containing the soluble part of the HIV receptor CD4 coupled to the constant part (Fc) of human IgG1 immunoglobulin was synthesized and tested as an anti-HIV reagent in vitro. The molecule may combine the specific binding properties of CD4 to the virus with functions of
Sporulation promoter SpovG controlled expression of P41 gene of HIV-1 in bacillus megaterium.: Int Conf AIDS 1989 Jun 4-9; 5:674 (abstract no. C.698); Ginsburgh C, Spaulding D, Robey G, Shivakumar A, McCall O, Vanags R; Abbott Laboratories, North Chicago, Illinois, USA; Expression of full length recombinant HIV-1 p41 protein from E. coli results in marginal production of a partially degraded protein. Here we describe the successful expression of this full length hydrophobic protein from a gram positive bacteria. The promoter Pspo VG for the gene encoding the sporul
Histopathological study of HIV associated non Hodgkin's lymphomas. The value of morphological classifications.: Int Conf AIDS 1989 Jun 4-9; 5:675 (abstract no. C.699); Raphael M, Tulliez M, Henin D, Berger F, Lenoir G, Diebold J; Pitie-Salpetriere, Paris, France; High grade of malignancies Non Hodgkin s Lymphomas (NHL) are associated with AIDS. Using the international working formulation and Kiel classification, large cell, Burkitt s and Immunoblastic NHL were recognized. OBJECTIVES AND To describe NHL in AIDS patients, a multicentric study was organized to obtain slid
Aids-associated early stage synodical Kaposi's sarcoma (KS) of the knee.: Int Conf AIDS 1989 Jun 4-9; 5:675 (abstract no. C.700); Mesquita RP, Bauer PG, Basilio CA, Oliveira RL, Sion FS, Sa CA; Virology, FIOCRUZ, Box 926, Rio, Brasil; To describe by optical and ultrastructural techniques a synodical KS with evidences of lymphaticovenous anastomoses. Light and electromicroscopy were applied to synovial biopsy specimens of the knee of a 26 year old HIV infected homosexual man with clinical diagnosis of polyarthritis (knee, ankle an
Absence of antibodies to HTLV-I/II in French patients with hematological malignancies.: Int Conf AIDS 1989 Jun 4-9; 5:675 (abstract no. C.702); Agius G, Vaillant V, Biggar R, Ranger S, Dindinaud G, Castets M; CHU La Miletrie, Poitiers, France; To evaluate 334 (191 males and 143 females) French patients with hematological malignancies for evidence of HTLV-I/II reactivity. The leukemia and lymphoma cases included 163 and 132 cases, respectively. None of these patients was diagnosed as having adult T-cell leukemia/lymphoma. Other cases inclu
Genetic analysis of Burkitt's lymphoma cells from a patient with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:675 (abstract no. C.703); Rasheed S, Yao KX, Zhou JT; Laboratory of Viral Oncology and AIDS Research, USC School of Medicine, Los Angeles, CA, USA; To identify genetic sequences that may contribute to the development of B-cell lymphoma in patients with AIDS. B-Lymphocytes naturally infected with the Human Immunodeficiency Virus (HIV) were established in vitro from the peripheral blood of patients with AIDS-related lymphoma. Cells were character
AIDS lymphoma: regulation of cell proliferation.: Int Conf AIDS 1989 Jun 4-9; 5:675 (abstract no. C.704); Feigal EG, Brown N, McGrath M, Greenspan JS; School of Medicine, University of California San Francisco (UCSF), San Francisco, CA, USA; To establish AIDS associated non-Hodgkin s lymphoma (NHL) cell lines in stable culture and determine their clonality with the original tumor tissue. To determine cell surface antigenic phenotype, the role of DNA tumor viruses, the effects of lymphokines and monokines on cell proliferation and whether recepto
Lymphokines in different stages of HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:676 (abstract no. C.705); Espanol T, Figuera T, Quer J, Garcia X, Caragol I, Ocana I; R.S. Valle Hebron. Barcelona, Spain; HIV causes a progressive loss of CD(4) cells and their functions, subsequently IL-2 synthesis is decreased with the evolution of the disease. Although monocytes are also infected there is no cytolysis of this cells, and then we were interested to know the synthesis of IL-1 in different stages of this infection and the
HIV-2 chronic infection of promonocytic cells.: Int Conf AIDS 1989 Jun 4-9; 5:676 (abstract no. C.706); Innocenti P, Brion JP, Baccard C, Seigneurin JM; Virology Laboratory, Centre Hospitalier Universitaire, Grenoble, France; To establish chronic infection in cells of the mononuclear phagocyte system, with HIV-2, and compare it to HIV-1 infection; to study the phenotypic variations throughout the infection. Two clones of the promonocytic cell line U 937, with 30% and 90% CD4+ respectively, were each infected with HIV-2 R
Suppressor effect of cell supernatants from HIV-positive patients on normal lymphocyte functions.: Int Conf AIDS 1989 Jun 4-9; 5:676 (abstract no. C.707); Estevez ME, Mariani L, Goicoa MA, Palacios F, Diez RA, Sen L; Seccion Inmunologia Oncologica, Instituto de Investigaciones Hematologicas, Buenos Aires, Argentina; The aim of the study was to determine if ConA can induce an increase of the NK cell activity of HIV-infected subjects (IS) similar to that of normal subjects (NS). Also, we investigated if the factors that normally regulate the NK function, released by cells from IS differ from those released by NS cells. ME
AIDS - panergic imnesia as pathogenic mechanism.: Int Conf AIDS 1989 Jun 4-9; 5:676 (abstract no. C.708); Ascher M, Sheppard H; Viral and Rickettsial Disease Laboratory, Berkeley, CA, USA; We propose a model whereby HIV component(s) cause immune dysfunction by mimicry of the physiologic ligands of CD4, molecules of the Major Histocompatibility Complex (MHC), binding to CD4 molecules and causing lymphocyte activation of a generalized and uncontrolled nature (panergy). The symptoms of mediator disease, aut
Characterization of human B cell (DK) and promonocyte (U937) after HIV-1 exposure.: Int Conf AIDS 1989 Jun 4-9; 5:676 (abstract no. C.709); Sei Y, Bekesi JG, Arora K; Laboratory of Neuroscience, NIDDK, National Institutes of Health, Bethesda, MD, USA; Comparison of HIV-1 infection-kinetics among human B (DK), promonocyte (U937), T-B hybrid (CEMx174), and T(H9) cell lines. SupT1 (HIV1 susceptible CD4+T) cells were used as targets to analyze syncytia induction capacity of various cell lines. Reverse transcriptase (RT) activity in the supernatant (e
Detection of CD4/CD8 expression on HIV infected T cell cultures.: Int Conf AIDS 1989 Jun 4-9; 5:676 (abstract no. C.710); McLane MF, Lee TH, Ito S, Wolf A, Farrar GH, Essex M; Harvard Univ., Boston, MA USA; The study describes the expression of CD4/CD8 on T cell culture lines at early and chronic stages of infection. We examined T cell cultures by the techniques of dual color immunofluorescence flow cytometry, ferritin labelling for election microscopy visualization and reverse transcriptase for virus
Biological role of cocaine in the development and expression of AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:677 (abstract no. C.711); Bagasra O, Forman LJ; Department of Microbiology, University of Medicine and Dentistry of New Jersey, Camden, New Jersey, USA; To determine the effect of cocaine on the immune system. In order to obtain a better understanding of the effects of cocaine on immune function, inbred male rats were treated daily for ten days with 1.25, 2.5 or 5.0 mg/kg of cocaine. Rats were then immunized with T-helper independent (TI) and T-help
Characteristics of HIV-1 in monocyte-macrophages.: Int Conf AIDS 1989 Jun 4-9; 5:677 (abstract no. C.712); Shunji M, Gidlund M, Nilsson K, Fenyo EM, Wigzell H; Karolinsa Institute, Stockholm, Sweden; To investigate infectivity and localization of HIV in monocyte-macrophages. HTLV-IIIB producing cells, U937 subclones, THP-1 and T cell lines, were used for this experiment. HIV-1 in the cells were collected after mechanical destruction of cells by freezing and thawing. Amount of HIV-1 in the soluti
Presence of human immunodeficiency virus (HIV) in the alveolar macrophages (AM) from bronchoalveolar lavage (BAL) fluid of patients with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:677 (abstract no. C.713); Linnemann CC, Baughman RP, Frame PT, Floyd R; Depts. of Medicine, Pathology & Laboratory Medicine, University of Cincinnati Medical Center, Ohio, USA; Previous work in our laboratory has shown that HIV can be recovered from the BAL fluid of all patients with AIDS, but has only detected p24 antigen directly in the BAL fluid of a patient with lymphocytic interstitial pneumonitis. To determine which cells in the BAL fluid contain the virus, BAL cells were separated into
Possible role of genetic, immunological and viral factors in the acquirement and/or progression of HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:677 (abstract no. C.714); Purpura M, Rosci MA, Miceli M, Mirolo M, Pezzella M, Mannella E; National Centre for Blood Transfusion CRI, Rome, Italy; To evaluate the role of genetic, immunological and viral factors in the acquirement and/or progression of HIV infection. Five seronegative regular sexual partners of HIV seropositive subjects and 5 HIV seropositive patients without HIV infection progression (during the last 5 years, were included in
HIV-1 infection of human fetal thymocytes.: Int Conf AIDS 1989 Jun 4-9; 5:677 (abstract no. C.715); Tanaka K, Hatch W, Rashbaum W, Rubinstein A, Soeiro R, Lyman WD; Albert Einstein College of Medicine, Bronx, NY, USA; Some infants with congenital HIV-1 infection are immunocompromised. This suggests that fetal thymocytes may become infected by HIV-1 resulting in immune dysfunction. To examine this hypothesis, normal human fetal thymocytes were established in culture and exposed to HIV-1. The potential for this virus to aff
AIDS is a non-infectious "occupational" syndrome.: Int Conf AIDS 1989 Jun 4-9; 5:677 (abstract no. C.716); Moretti ML; Instituto de Terapia Fisica y Medicina Interna de Venezuela, Caracas, D.F., Venezuela; To find answers to the following questions: a) Is AIDS an infectious disease? b) Can it be considered a sexually transmitted disease? c) Is HIV the etiological agent? d) What is the prophylaxis for AIDS? e) What is the possible cure for people at high risk for AIDS, with PGL, ARC or AIDS? Three year
Time course of IGG reactivity with synthetic peptides derived from HIV-1 gag and env in HIV-1 seropositive individuals.: Int Conf AIDS 1989 Jun 4-9; 5:678 (abstract no. C.717); Blomberg J, Klasse PJ, Pipkorn R, Ljungberg B, Christensson B, Ursing B; Section of Virology, Dpt Med Microbiol, Sweden; To evaluate the development of the immune response to epitopes simulated by synthetic peptides during the course of HIV-1 infection. EIA:s with synthetic peptides from HIV-1 gag (1-20, 320-338, 335-351) and env (503-520, 581-599, 583-599, 586-606, 602-622, 848-863) were performed on consecutive sera
Pathogenesis of NK deficiency in AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:678 (abstract no. C.718); Sirianni MC, Tagliaferri F, Aiuti F; Dept. of Allergy and Clinical Immunology, University of Rome, Rome, Italy; To give a model explaining NK defect in AIDS. PBL from AIDS patients were assessed for NK activity on the 51Cr labelled K562 cell line. Cytoskeletal tubulin polarization in these conjugates was studied by monoclonal antibodies (MoAb) and immunofluorescence. Tubulin is responsible for an effective NK
A rapid and improved 2', 5' a synthetase assay by polyacrylamide gel electrophoresis.: Int Conf AIDS 1989 Jun 4-9; 5:678 (abstract no. C.719); Kan NC, Miele MB, Liu DK; Department of Pharmacology, The Pennsylvania State University, College of Medicine, Hershey, PA, 17033, USA; Induction of 2 , 5 oligoadenylate (2, 5A) synthetase activity is an accurate and sensitive parameter for viral infection of mammalian cells. It could be used clinically to monitor circulating interferons. The enzymatic assay ideally involves chromatographic separation of 2,5As, which range from di- to oligomers, from e
Kinetics of HIV infected cell-to-cell fusion.: Int Conf AIDS 1989 Jun 4-9; 5:678 (abstract no. C.720); Graham B, Rowland J; Vanderbilt University School of Medicine, Nashville, TN, USA; To define the mechanism and timing of the cell-to-cell fusion process important in the pathogenesis of AIDS. A cytopathic effect assay to measure serum fusion inhibition activity has been developed that utilizes H9/HTLV-IIIB as fusion effector and the CD4+cell line, MT2, as target for syncytium form
Tropical spastic paraparesis: serologic and histological analysis.: Int Conf AIDS 1989 Jun 4-9; 5:678 (abstract no. C.721); Canavaggio M, Leckie G, Chi L, Madrid RE, Lee H, Valderrama R; Abbott Laboratories, North Chicago, IL, 60064, USA; To identify and further characterize by sural nerve biopsies, HAM/TSP in a neurological patient population from New York City. Serum and CSF from 150 patients were assayed for antibodies to HTLV I by EIA. Repeat reactives were confirmed by Western blot and SDS-PAGE RIPA. WB was also performed on pai
Host immunoglobulin molecules are on the surface of Pneumocystis carinii.: Int Conf AIDS 1989 Jun 4-9; 5:678 (abstract no. C.722); Blumenfeld W, Griffiss JM; Veterans Administration Medical Center, University of California, San Francisco USA; To define the organism and/or host factors that explain the pathogenesis of P. carinii pneumonia in AIDS. (1) Immunochemical labeling of whole organism preparations of P. carinii in bronchoalveolar lavage fluid sediment with enzyme-tagged polyclonal and monoclonal antibodies to human immunoglobulins
Infection of monocytes with a t-cell tropic clone of HIV.: Int Conf AIDS 1989 Jun 4-9; 5:678 (abstract no. C.723); Justement JS, Folks TM, Orenstein J, Fauci AS; LIR, NIAID, NIH, Bethesda, MD, USA; To determine if an HIV clone derived from infected T cells can infect normal human monocytes in vitro. The HIV infectious clone (pNL4-3) was developed by combining the 5 GAG-POL region of the NY5 strain construct and the 3 ENV region of the LAV strain construct. The pNL4-3 construct was transfected
Monocyte chemotactic activity in the supernatants of in vitro infected human macrophages.: Int Conf AIDS 1989 Jun 4-9; 5:679 (abstract no. C.724); Lazdins JK, Alteri E, Meltzer MS; Research Laboratories, Pharmaceutical Division, CIBA-GEIGY Ltd. CH-4002 Basel, Switzerland; Macrophages (Mphi) are an important target for HIV infection and probably play a major role in the establishment and propagation of AIDS. Following infection, the virus is found in tissue Mphi and blood monocytes. At present, the mechanism of infection and spreading of HIV in these cells is not known. We present result
Detection of HTLV-1 in alveolar lymphocytes of patients with tropical spastic paraparesis (TSP) with bronchoalveolar lymphocytosis.: Int Conf AIDS 1989 Jun 4-9; 5:679 (abstract no. C.725); Desgranges C, Couderc LJ, Noraz N, Caubarrere I, Vernant JC; INSERM U 271, Lyon, France; To specify the type of alveolar cells detaining HTLVI genome in the TSP patients with bronchoalveolar lymphocytosis. METHOD: After bronchoalveolar washing of TSP patient serologically HTLV I associated, with bronchoalveolar lymphocytosis, the adherent and non-adherent cells were separated, identified and HTL
Plasma values of fibronectin (FN) and angiotensin converting enzyme (ACE) in Kaposi's sarcoma (HIV-related and classical).: Int Conf AIDS 1989 Jun 4-9; 5:679 (abstract no. C.726); Janier M, Drouet L, Villette JM, Scrobohaci ML, Palangie A, Morel P; STD Clinic, Paris, France; To investigate plasma values of FN and ACE as putative markers of endothelial proliferation in HIV-related and classical Kaposi s sarcoma (KS). Plasma FN (ELISA, Stago Lab) and ACE (Radiochemical technique of Rohrbach) were determined in patients with AIDS-related KS, classical KS and the lymphadeno
CD4+ cell helper and suppressor function in HIV infected patients.: Int Conf AIDS 1989 Jun 4-9; 5:679 (abstract no. C.727); Weimer R, Daniel V, Schweighoffer T, Schimpf K, Opelz G; Institute of Immunology, University of Heidelberg, and Rehabilitation Hospital and Hemophilia Center, Heidelberg, FR Germany; To investigate helper and suppressor functions of CD4+ T cells in HIV infected hemophilia patients. We assessed T cell functions in 61 hemophilia patients (21 HIV-, Group 1; 27 HIV+ without ARC/AIDS, Group 2; 13 ARC/AIDS patients, Group 3). PWM-stimulated allogeneic cocultures of CD4+ T cells and co
On the pathogenesis of severe HIV-related thrombocytopenia (TP) in intravenous drug abusers (IVDAS).: Int Conf AIDS 1989 Jun 4-9; 5:680 (abstract no. C.729); Landonio G, de Cataldo F, Gargantini L, Nosari AM, Galli M, Lazzarin A; Department of Haematology, Niguarda Ca'Granda Hospital, Milan and Clinic of Infectious Disease, University of Milan, Milan, Italy; To evaluate the pathogenesis of severe HIV related TP in IVDAs, by kinetic studies of platelet survival. Platelet kinetic study was carried out in 20 HIV-positive IVDAs with severe TP (Plt less than 25 X 10 9/L) by 111-InOxine labelled autologous platelets (method of Thakur, partially modified). The
HIV-2 neutralization epitopes are localized in the carboxyl terminal portion of the external envelope.: Int Conf AIDS 1989 Jun 4-9; 5:480 (abstract no. C.732); Goudsmit J, McKeating J, Meloen R, Smit L, Bakker M, Barin F; Human Retrovirus Laboratory, Amsterdam, the Netherlands; To localize HIV-2 external envelop domains binding neutralizing antibodies. Five hundred overlapping peptides that covered the complete amino acid sequence of the external envelope of the HIV-2 ROD strain were synthesized with the antibody-reactive peptide scanning (PEPSCAN) technique. Sera of HIV-2
Variable stringency hybridisation of PCR amplified HIV-1 DNA fragments.: Int Conf AIDS 1989 Jun 4-9; 5:680 (abstract no. C.733); Snart R, Kolesnitchenko V, Agius G, Zagury JF, Zagury D; Universite Pierre et Marie Curie, Paris, France; DNA isolated from the lymphocytes of atypically seropositive individuals has been used for PCR amplification of envelope regions expressing atypical antigens. Eight aliquots of the amplified DNA fragment have been subject to Southern/Dot blot analysis, hybridising with 32P labelled B10 at 45 degrees C using 2ssc medium
Neutralizing epitope of HIV-1: antibody specificity, immunogenicity, and protein localization.: Int Conf AIDS 1989 Jun 4-9; 5:681 (abstract no. C.736); Murphy D, Shih JW, Berkower I; Lab. of Molecular Immunology, FDA and Dept. of Transfusion Med., NIH, Bethesda, MD, USA; Antibodies from seropositive patients reduced the number of infectious HIV-1 plaques in a new plaque forming assay. Several HIV-1 isolates (IIIB, RFII, and Z-84) were incubated with sera from asymptomatic seropositive patients and then used to infect a CD4+ HIV susceptible monolayer. Neutralization of IIIB and Z-84 was
Direct detection of a newly identified virus-like infectious agent by PCR in blood (PBL) from patients with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:681 (abstract no. C.738); Wang RY, Pierce PF, Wong DM, Hayes MM, Shih JW, Lo SC; Armed Forces Institute of Pathology, Wash, D.C. USA; To study the infection of the recently discovered virus-like infectious agent (VLIA) (Am J Trop Med Hyg, 40(2), 1989) in patients with AIDS, using the highly sensitive technique of selective gene amplification. Peripheral blood lymphocytes (PBL) from 23 patients with AIDS and 22 normal healthy donor
HIV genome in seronegative regular sexual partners of HIV infected subjects.: Int Conf AIDS 1989 Jun 4-9; 5:681 (abstract no. C.739); Pezzella M, Mirolo M, Rosci MA, Vonesch N, Miceli M, Mannella E; Institute Infectious Diseases, Policlinico Umberto I, University of Rome, Italy; The aim of our research was to evaluate the presence of HIV genome in peripheral blood mononuclear cells (PBMCs) from 15 seronegative (ELISA test Western Blot confirmed) regular sexual partners of HIV infected subjects. The T4/T8 ratio, determined by Flow Cytometer, ranged between 0.08 - 2.10. PBMCs
Serial antibody (ABY) reactions to human herpesvirus-6 (HHV-6) in HIV-1 infection.: Int Conf AIDS 1989 Jun 4-9; 5:681 (abstract no. C.740); Kaplan MH, Hsieh HC, Coronesi M, Ablashi D, Salahuddin SZ; North Shore University Hospital, Cornell University, Manhasset NY, USA; To study Aby responses to HHV-6 in HIV-1 infection. Sera collected serially over the course of 1-4 years from a cohort of HIV infected patients (pt) was tested for Aby to HHV-6 strain Da. Virally infected HsB2 and uninfected cells were fixed to slides and tested for IgG and IgM antibody using immuno
Macrophages simultaneously derived from virus-containing peripheral blood mononuclear cells (PBMC) in HIV-1 IIIB infected chimpanzees do not contain virus.: Int Conf AIDS 1989 Jun 4-9; 5:682 (abstract no. C.741); Nara PL, Hatch W, Kessler J, Kelliher J, Ehrlich G, Gendelman H; National Cancer Institute, Frederick, Maryland, USA; To extend our previous in vivo and in vitro studies of the fate and biology of the HIV-1 IIIB virus in persistently infected chimpanzees. Various sub-populations of cells were derived from different anatomic sites in persistently infected chimpanzees. These were evaluated for viral expression by co-
Characterization of HIV virosomes.: Int Conf AIDS 1989 Jun 4-9; 5:682 (abstract no. C.742); Bernard C, Vandenbranden M, Cogniaux J, Dekegel D, Ruysschaert JM; Universite Libre de Bruxelles, Belgique; Topology of HIV viral proteins in reconstituted systems (virosomes). After virus lysis in OGP and addition of exogenous lipids, virosomes were obtained by dialysis of detergent. Virosomes were layered on top of continuous glycerol gradient and ultracentrifuged (120000g, 16h.) in order to remove free
Biological characterization of HIV isolates from different Brazilian AIDS patients.: Int Conf AIDS 1989 Jun 4-9; 5:682 (abstract no. C.743); Couto-Fernandez JC, Ivo-dos-Santos J, Galvao-Castro B; Department of Immunology, Oswaldo Cruz Foundation, Ministry of Health, WHO Collaborating Centre, Rio de Janeiro, Brazil; To evaluate the biological characteristics such as cytophatic effect (CPE), syncytium formation and replication rate of five isolates obtained from Brazilian patients with different clinical forms of infection in cells of lymphoid and monocytoid origin. Lymphoid (H9, CEM,MT-02, Jurkatt), monocytoid
Geographical variation in cross reactivity of HIV2 sera on HIV1 assay.: Int Conf AIDS 1989 Jun 4-9; 5:682 (abstract no. C.745); Simon F, Peeters M, Andrade D, Theobald S, Maiga Y, Cot MC, Brun-Vezinet F; Laboratoire de Virologie, Hopital Bichat-Claude Bernard, Paris, France; To study the reactivity of HIV2 positive sera on HIV1 antigens according to the geographical origin of the patients. We tested HIV2 positive sera collected in subjects from Cape-Verde Islands (n=64) and Mali (n=68). The 64 Cape Verdian sera included all the specimens detected as HIV2 positive in the
Chemical inactivation of purified SIV with B-propiolactone and formalin.: Int Conf AIDS 1989 Jun 4-9; 5:682 (abstract no. C.746); Keddie EM, Jennings MB, Carlson JR; School of Medicine, University of California, Davis, USA; B-propiolactone (B-PL) and formalin were tested for ability to inactivate Simian Immunodeficiency Virus (SIV). 500 ug lots of sucrose-gradient purified SIVmac were treated with 0.025%, 0.1%, and 0.2% B-PL for 5 hours at 4C. One 500 ug sample was treated with 0.025% formalin at 30C for 48 hours. One
HBV infection in HIV infected subjects.: Int Conf AIDS 1989 Jun 4-9; 5:683 (abstract no. C.747); Ferrazzi M, De Rinaldis ML, Vonesch N, Sturchio E, Mannella E, Pezzella M; Institute Infectious Diseases, Policlinico Umberto I, University of Rome, Italy; The aim of our study was to investigate the concomitant HBV infection in HIV infected subjects. We observed 143 symptomatic and asymptomatic anti-HIV seropositive subjects (ELISA test, Western blot confirmed). All patients were tested for HBV antigens (HBsAg and HBeAg), for antibodies (anti-HBs, ant
HIV-1 infection of human fetal dorsal root ganglia glial cells is independent of the T4A epitope of CD4.: Int Conf AIDS 1989 Jun 4-9; 5:683 (abstract no. C.749); Wigdahl B, Hartle HT, Kunsch C, Siever D; Department of Microbiology and Immunology, The Pennsylvania State University College of Medicine, Hershey, PA 17033 USA; To examine the role of CD4 or related molecules in infection of human fetal dorsal root ganglia (DRG) glial cells by human immunodeficiency virus type 1 (HIV-1). Monoclonal antibodies directed against the CD4 protein were used to inhibit infection of primary human fetal DRG glial cell cultures by HI
Utilization of syncytium-formation microassay for quantitation of infective Human Immunodeficiency Virus type 1 (HIV-1).: Int Conf AIDS 1989 Jun 4-9; 5:683 (abstract no. C.750); Hwang MY, Ting RC, Kaneko Y, Aoki T; Biotech Research Laboratories, Inc., Rockville, MD, USA; To describe a method for quantitating infective HIV-1. METHOD: Detection of syncytium formation in the infected CEM-SS cells cultured in an adhesive-protein coated, 96-well plate. The syncytium formation was compared with the production of HIV-1 core antigen--p24. RESULT: Infective HIV-1 was detected and rel
Continuous epitopes on GP 120 with special reference to the CD-4 binding site.: Int Conf AIDS 1989 Jun 4-9; 5:683 (abstract no. C.752); Anders GN, Fenyo EM, Norrby E; Department of Virology, Karolinska Institute, Stockholm, Sweden; To study continuous epitopes on GP 120. A large panel of sera was obtained from guinea pig immunised with synthetic peptides representing various regions of GP 120. These sera were tested for binding to native, fully glycosylated GP 120. RIPA, inhibition of binding between GP 120 and CD-4 and testin
Growth of HIV in cells from various donors.: Int Conf AIDS 1989 Jun 4-9; 5:684 (abstract no. C.753); Yamada O, Matsumoto T, Sakaoka R, Kurimura T; Department of Virology, Tottori University School of Medicine, Yonago 683, Japan; Peripheral blood mononuclear cell (PBMC) preparations were prepared from 27 individuals and the growth of Japanese HIV isolates, JH/3 and JMH/1, in those PBMC preparations was studied. There was significant diversity in infectious virus yield among PBMC preparations. The virus yield did not correlate with the ratio of
Detection by polymerase chain reaction of HIV-1 DNA in homosexuals, hemophiliacs, and AIDS patients in Taiwan.: Int Conf AIDS 1989 Jun 4-9; 5:684 (abstract no. C.754); Guo H, Chuang CY, Shen MC, Lin HC, Ko YC, Chien CH, Chang KS; National Cancer Institute, NIH, Bethesda, MD, USA; To detect HIV-1 proviral sequences by polymerase chain reaction (PCR) with DNA samples obtained from seropositive Taiwanese subjects. Peripheral lymphoid cells from an AIDS patient, an asymptomatic homosexual male, and two hemophiliacs, as well as formalized spleen tissues from two autopsied AIDS pa
Construction of a recombinant retroviral interfering particle containing a defective HIV-1 genome.: Int Conf AIDS 1989 Jun 4-9; 5:684 (abstract no. C.755); Federico M, Taddeo BM, Orecchia A, Saggio I, Carlini F, Verani P, Rossi GB; Laboratory of Virology, Istituto Superiore di Sanita, Rome, Italy; By infecting Hut-78 cells with an RT positive supernatant of PBL from an AIDS patient, we have isolated a non-producer HIV-infected cell clone (F12), which exhibits a viral RNA pattern superimposable with that of productive cell clones, and shows resistance to HIV-1 or HIV-2 superinfection. In order to study
Replication of a lethal variant of SIV/SMM in macaque PBMC in the absence of exogenous IL-2 or activation by mitogens.: Int Conf AIDS 1989 Jun 4-9; 5:684 (abstract no. C.758); Fultz P, McClure H, Anderson D; Yerkes Regional Primate Research Center and Department of Pathology, Emory University, Atlanta, GA, USA; To identify mechanisms by which SIV/SMM (PBj(14)) replicates in and induces proliferation of PBMC from normal macaque and mangabey monkeys. Resting or PHA- stimulated PBMC from normal monkeys, chimpanzees or humans were infected with SMM (PBj(14)) or the parent, SIV/SMM-9; cultures were monitored fo
HIV-1 sequence analysis of brain and blood lymphocytes from neurologically symptomatic AIDS patients using PCR techniques.: Int Conf AIDS 1989 Jun 4-9; 5:685 (abstract no. C.759); Levy R, Gurney M, Wolinsky S; Northwestern University Medical School, Chicago, IL, USA; To determine whether there are specific neurotropic strains of HIV-1 which result in brain dysfunction and the location of possible functional gene loci conferring neurotropism and neuropathogenicity, we have isolated and partially characterized HIV-1 from the brains and blood lymphocytes of six AIDS patients with neur
The morphology of a new lentivirus.: Int Conf AIDS 1989 Jun 4-9; 5:685 (abstract no. C.760); Koch MG, Marx PA, Bykovsky AF, Strandberg B, Nilsson L; Vardcentralen, S-546 00 Karlsborg, Sweden; HIV is, to the best of our knowledge, the first lentivirus ever to infect mankind. We show the unveiling of its structure by scanning and transmission electron microscopy, by thin section and tilt series, by stroboscopic analysis of surface symmetries under rotation, by crystallographic and computer based structural an
Anti-HIV-IgG productions in vitro during virus isolation.: Int Conf AIDS 1989 Jun 4-9; 5:683 (abstract no. C.761); Vanderborght B, De Leys RJ, Willems B, Nijs P, Van der Groen G, Van Heuverswyn H; Innogenetics N.V. Antwerpen, Belgium; Influence of anti-HIV production in vitro on HIV-isolation. Fresh Peripheral Blood Lymphocytes (PBLs) of 45 European ARC patients were collected monthly and cocultivated with PHA stimulated PBLs from healthy donors. 136 virus cultures were screened for anti HIV-IgG production after 3 and 14 days. Sc
Variant retrovirus infection in African primates.: Int Conf AIDS 1989 Jun 4-9; 5:685 (abstract no. C.762); Delphi M, Bernard J, Ittelet D, Gallo RC, Zagury D; Universite P et M. Curie, Paris, France; To define the extent of retrovirus infection in the primate population of East Zaire , then to isolate and characterize retroviral variants from seropositive species. All available sera from wild monkeys were tested for HTLV1, HIV1 and HIV2 reactivity using Western Blot analysis. Sera from
HIV-like particles in neurons of the brain.: Int Conf AIDS 1989 Jun 4-9; 5:685 (abstract no. C.763); Bauer PG, Sa CA, Sion FS, Basilio CA, Fialho F, Valle HA; Virology, FIOCRUZ, Box 926, Rio, Brazil; To identify HIV infected neurons by electron microscopy. Specimens of brain autopsies of patients which died of AIDS were prepared for routine electron microscopy and systematically analysed. Investigating several hundred neurons on ultrathin sections we could observe on rare occasions an H
Reactivity of human HIV-2 antibodies with native and synthetic HIV-1 and HIV-2 antigens.: Int Conf AIDS 1989 Jun 4-9; 5:685 (abstract no. C.764); Huskins KR, Furness JN, Reagan KJ, Neumann JE, Hu YW, Kang CY; E. I. Du Pont Co., Glasgow 713, Wilmington, DE, 19898, USA; To characterize the reactivity of human HIV-2 antibodies with viral antigens and synthetic polypeptides from the gag, env, and pol genes of HIV-1 or HIV-2 as a means to distinguish HIV-2 infection from HIV-1. Samples confirmed positive for HIV-2 antibodies by differential response on whole virus Wes
[Screening and typing of HIV-1 and HIV-2 by a rapid test (3 minutes) using synthetic peptides (clonatec target HIV test]: Int Conf AIDS 1989 Jun 4-9; 5:686 (abstract no. C.765); Ranger S, Leonard G, Bettinger S, Sangare A, Gershy-Damet GM, Denis F; Departement Virologie, CHU Limoges, France
Absence of P24 antigen and anti P24 antibody decreasing in African AIDS: comparison between European and west African populations.: Int Conf AIDS 1989 Jun 4-9; 5:686 (abstract no. C.766); Leonard G, Weinbreck P, Mounier M, Gershy-Damet GM, Barin F, Denis F; Virology Departments, CHU Limoges France; To compare virological prognostic factors (p24 antigenemia, anti p24 antibody titer) in European and west African patients. MATERIAL AND 508 HIV positive patients from Limoges France (106 sera) and Abidjan Cote d Ivoire (402 sera) were tested for p24 antigenemia by Abbott HIV-1 antigen p24 assay and
Influence of blood volume and transport on HIV cultures in infants and children.: Int Conf AIDS 1989 Jun 4-9; 5:686 (abstract no. C.767); Robb ML, Vincent J, Flower A, Dilworth S, Zawadsky P, Burke D; Walter Reed Army Medical Center, Washington, D.C., USA; To determine the influence of blood volume and specimen transport on pediatric HIV cultures. Blood cultures for HIV were obtained with 1-3 cc of heparinized whole blood from 28 patients (PO-P2) with an age range of 3 mos. to 11 yrs. Each culture was evaluated with a standard number of the patient s
Primary isolation of HIV from lymphocytes, monocytes, and macrophages with a permanent T-cell line as a model to study virus replication and cell tropism in vitro.: Int Conf AIDS 1989 Jun 4-9; 5:686 (abstract no. C.768); Baur A, Ehrmann U, Jahn G; Institut fur Klinische und Molekulare Virolgie der Universitat Erlangen-Nunberg, Klinische D-8520 Erlangen, FRG; To establish a standardized HIV isolation procedure where PMBC can be replaced by a permanent T-cell line in order to study primary HIV replication and transmission of HIV from monocytes/macrophages to T-cells. METHOD: Under optimal conditions primary HIV isolation gave an overall positive result in 84%. Usi
Human immunodeficiency virus (HIV) multiplies in primary cultures of human Kupffer cells.: Int Conf AIDS 1989 Jun 4-9; 5:686 (abstract no. C.769); Schmitt MP, Steffan AM, Jaeck D, Gendrault JL, Kirn A; Virus Department and INSERM U 74, Strasbourg, France; Given their localisation in the liver sinusoid, the Kupffer cells (KC), which represent the largest reservoir of fixed macrophages in the body, are ready candidates for early infection following contamination. However, until now, the role of KC in HIV infection has never been studied and no attempt has ever
Productive infection of primary cultures of human hepatic endothelial cells (EC) by human immunodeficiency virus (HIV).: Int Conf AIDS 1989 Jun 4-9; 5:686 (abstract no. C.770); Steffan AM, Schmitt MP, Gendrault JL, Jaeck D, Kirn A; Virus Department and INSERM U 74 Strasbourg, France; The presence of CD 4 antigen on the surface of human liver EC renders them susceptible to infection by HIV. Hepatic EC were isolated from liver specimens obtained after partial hepatectomy in secondary liver cancer by a method we developed several years ago (Steffan et al., C.R. Acad. Sci. Paris, 19
A human monoclonal antibody against HIV-1 transmembrane glycoprotein generates complement-mediated, antibody-dependent enhancement (C'-ADE) of HIV-1 infection in vitro.: Int Conf AIDS 1989 Jun 4-9; 5:687 (abstract no. C.771); Robinson WE Jr, Gorny MK, Xu JY, Montefiori DC, Mitchell WM, Zolla-Pazner S; Dept. of Pathology, Vanderbilt University, Nashville, TN, USA 37232; To evaluate ten human monoclonal antibodies (mAb) against HIV-1 for their abilities to neutralize or enhance HIV-1 infection in vitro. MATERIALS: Ten human lymphoblastoid cell lines producing mAb against HIV-1 were generated in vitro by infection of peripheral blood lymphocytes from HIV-1 seropositive subjec
Identification of protein intermediates in the processing of the p55 HIV-1 gag precursor.: Int Conf AIDS 1989 Jun 4-9; 5:687 (abstract no. C.772); Gowda SD, Stein BS, Engleman EG; Stanford University School of Medicine, Palo Alto, CA 94304, USA; To analyze the cleavage pathway of the 55 kDa (p55) HIV-1 gag precursor. HIV-1 gag proteins isolated from [3H] leucine labeled, recombinant HIV-1/vaccinia virus infected H9 T lymphocytes by immunoprecipitation with anti-p24, anti-p17 or anti-p6 antibodies were subjected to one- and two-dimensional S
Early diagnosis of HIV infection in children and newborn by lymphocyte cultures and DNA/RNA detection in the polymerase chain reaction (PCR).: Int Conf AIDS 1989 Jun 4-9; 5:687 (abstract no. C.773); Escaich S, Philippe N, Ritter J, Clarisse O, Baginski I, Trepo C; INSERM U 271, Lyon, France; In order to diagnose early HIV infection in newborn and children from seropositive mothers, we have looked for HIV in their peripheral blood mononuclear cells (PBMC). 16 seropositive children were studied, age one day to 2 years and HIV was detected in lymphocyte cultures by P24 Ag ELISA (Abbott). H
Comparison of single and double-labelling of SLB1 and MT2 cells in the detection of HTLV-1 antibodies by radioimmunoprecipitation assay.: Int Conf AIDS 1989 Jun 4-9; 5:687 (abstract no. C.775); Sullivan MT, Williams AE, Slamon DJ; American Red Cross, Rockville, MD, USA; 1. To determine if the sensitivity to HTLV-1 antibodies can be enhanced by a double-labelled lysate of infected cells. 2. To evaluate Leu as a substitute for Cys in the labelling of viral antigens. 3. To compare RIPA profiles from MT2 cells to a cell line of non-Asian origin (SLB1). Samples known to
High capacity drug screen for inhibitors of HIV tat transactivation: effect of interferons.: Int Conf AIDS 1989 Jun 4-9; 5:687 (abstract no. C.776); Bacheler L, Nardone B; Medical Products Department, E.I.DuPont de Nemours and Co. Inc., Experimental Station, Wilmington De 19880-0400, USA; Transactivation of the HIV LTR by the virally encoded tat protein is an essential step in virus replication. As such, tat transactivation is an attractive target for antiviral therapy. Transfection of HeLa cells with recombinant DNA plasmids expressing the HIV tat protein and linking E. coli beta-galactosidase to the H
Serologic analysis of proteins associated with and preliminary seroprevalence studies of two novel retroviruses isolated from AIDS-associated lymphomas.: Int Conf AIDS 1989 Jun 4-9; 5:688 (abstract no. C.777); Ng VL, Khayam-Bashi F, Marsh J, McGrath MS; University of California, San Francisco, and San Francisco General Hospital, San Francisco, California, USA; To determine the serologic relationship of the proteins associated with two novel retroviruses, 2F7 and 10C9, isolated from primary AIDS-associated lymphoma tissue to other known retroviruses, and to determine prevalence of exposure to these viruses in various populations at risk for retroviral infection. M
Budding features of different morphologies of HIV-1 particles from cell clones isolated from HIV-1 infected MT-4 cells.: Int Conf AIDS 1989 Jun 4-9; 5:688 (abstract no. C.778); Goto T, Ikuta K, Zhang JJ, Kato S, Nakai M; Osaka Medical College, Osaka, Japan; To elucidate the viral maturation process, Cell clones were isolated from HIV-1-infected MT-4 cells. Cloned cells were observed by electron microscopy, and immunoelectron microscopy was also carried out using monoclonal antibodies and HIV-1-seropositive serum. The cell clones produced parti
Non-specific HIV 24 antigen reaction due to bacterial contaminants.: Int Conf AIDS 1989 Jun 4-9; 5:688 (abstract no. C.779); Rangan SR, Pirkle S, Impey A, Janney A, Hyslop NE; Tulane University School of Medicine, New Orleans, LA, USA; To determine the source of HIV p24 reactivity in a sample of tissue culture growth medium (TCM) used as matrix negative control in capture assay. A putative microbial contaminant in a macroscopically clear TCM was amplified at 37 degrees for 48 hrs. The microbe was re-isolated on blood-agar and iden
Macrophage cultures do not increase frequency of HIV isolation over lymphocytes from hemophiliacs.: Int Conf AIDS 1989 Jun 4-9; 5:688 (abstract no. C.780); Lemos RS, Rangan SR, Andes A, Hyslop NE; Tulane University School of Medicine, New Orleans, LA, USA; To determine whether macrophages (Mphi of hemophiliacs preferentially harbor HIV, since the frequency of virus isolation from hemophiliacs in many studies is lower than that from infected asymptomatic homosexuals. Monocytes and lymphocytes from asymptomatic seropositive hemophiliacs and homosexual c
Biological factors affecting transmission of human immunodeficiency virus.: Int Conf AIDS 1989 Jun 4-9; 5:688 (abstract no. C.781); Robertson JR, Skidmore CA, Davidson SJ, Rebus S, Aw D, Peutherer JF; Edinburgh Drug Addiction Study, Edinburgh, UK; To assess biological factors which affect heterosexual transmission of HIV, with particular reference to biological markers of infectivity. 15 couples were identified where one partner (13 male, 2 female) was infected with HIV, and the other confirmed seronegative. The couples had been in a sexual r
Expression and transactivation of HIV promoter in bacteria.: Int Conf AIDS 1989 Jun 4-9; 5:688 (abstract no. C.782); Wood C, Kashanchi F; Department of Microbiology, University of Kansas, Lawrence, Kansas, 66045 USA; To study the expression and transactivation of HIV long terminal repeats (LTR) in a bacterial system. A recombinant plasmid containing the HIV-LTR linked to an indicator gene, chloramphenicol acetyl transferase (CAT), was transformed into E. coli. The expression and transactivation of the HIV promot
Importance of structural elements of HIV GP120 to CD4-binding ability.: Int Conf AIDS 1989 Jun 4-9; 5:689 (abstract no. C.783); Hemming A, Bolmstedt A, Flodby P, Lundberg L, Olofsson S; Department of Clinical Virology, University of Goteborg, Goteborg, Sweden; The CD4-binding region of HIV gp120 has been mapped to a peptide stretch situated between aa 370 and aa 480. The aim of this study is to identify structures within this region, which are important for the ability of gp120 to bind to the CD4-receptor. Site-directed mutagenesis was used to introduce m
Human immunodeficiency virus (HIV) examined by negative staining: morphology and antigenicity.: Int Conf AIDS 1989 Jun 4-9; 5:689 (abstract no. C.784); Chrystie IL, Almeida JD, Banatvala JE; Department of Virology, United Medical and Dental Schools of Guys and St Thomas's, St Thomas's Campus, London, United Kingdom; Negative staining EM has proved less rewarding than thin sectioning in the study of HIV morphology and antigenic structure. This may, in part, be due to the close association of HIV with the propagating cell making it difficult to obtain good preparations of isolated virus. However, when HIV-2 infected cells (CB L-20/C
Failure to detect HIV-1 proteins and DNA in Entamoeba histolytica strains isolated from patients with HIV-1 infection.: Int Conf AIDS 1989 Jun 4-9; 5:689 (abstract no. C.786); Meyer CG, Burchard GD, Hufert FT, Berzow D, Schmitz H; Dpt. of Virology, Bernhard-Nocht-Institut for Tropical Medicine, Hamburg, FRG; To detect HIV proteins and proviral HIV DNA in Entamoeba histolytica strains, isolated from patients with HIV-1 infection and from E. histolytica cultures fed with HIV-1 infected lymphoblasts. In order to characterize E. histolytica strains from HIV infected persons, it is important to know whether these amo
Relationship of other infections to progression of disease in HIV infected men.: Int Conf AIDS 1989 Jun 4-9; 5:689 (abstract no. C.787); Leach C, Detels R, Giorgi J, English P, Cherry J; University of California at Los Angeles (UCLA) Schools of Public Health and Medicine, Los Angeles, California, USA; To determine the relationship of other infections to changes in immune status and risk of AIDS, specimens for isolation attempts for CMV , HSV-2, adenovirus, Ureaplasma urealyticum, and Mycoplasma hominis were collected 6 months apart in 222 HIV-1 antibody positive men participating in a cohort study of HIV-1 infection
Regulation of replication of HIV-1.: Int Conf AIDS 1989 Jun 4-9; 5:690 (abstract no. C.789); Haseltine W; Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA USA; Regulated growth of the Human Immune Deficiency Virus Type-1 (HIV-1) is the hallmark of infection with this virus. Prolific replication followed by a prolonged period of controlled replication followed once again by prolific replication is characteristic of most HIV-1 infections. The controlled aspect of the HIV-1 infe
Extraordinary without ordinary sexuality.: Int Conf AIDS 1989 Jun 4-9; 5:775 (abstract no. D.501); Smith L; Director Sexuality Division, Calgary Health Services, Calgary, Alberta, Canada; Education emphasizing negative aspects such as AIDS, STD s, unwanted pregnancy focuses on extraordinary sexuality . When negative information is stressed, feelings and behavioral strategies necessary for the integration of information and utilization of preventive actions are missed. Policy makers need to sh
HIV seroprevalence and demographic survey of incarcerated adolescents.: Int Conf AIDS 1989 Jun 4-9; 5:775 (abstract no. D.502); Baker CJ, Re O, Morris R, Huscroft S, Roseman J, Schultz B; Juvenile Court Health Services, Los Angeles County, California, USA; To determine HIV Seroprevalence and I.V. drug abuse factors in adolescents incarcerated within the Juvenile Halls, County of Los Angeles. Blood samples were collected anonymously from 2,005 youngsters. Also, the following were recorded: Age, Sex, Race, history of I.V. drug abuse and SGPT/SGOT testin
The use of one effective medium - television to educate Canadian youth and parents about HIV and AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:775 (abstract no. D.503); Liepold HM; Federal Centre for AIDS, Health and Welfare, Canada; As a result of the Canada Youth and AIDS Study conducted with 38,000 youth across Canada it was found that a high majority of Canadian youth received their information about AIDS from television. The Canada Youth and AIDS Study is representative of Canadian youth in grades 7, 9 and 11, first year University/College, dr
A model: targeted AIDS education and counselling for adolescents in an HMO.: Int Conf AIDS 1989 Jun 4-9; 5:775 (abstract no. D.504); Wibbelsman CJ, Schram N, Long O; Kaiser Permanente Medical Care Program, San Francisco, California USA; Within a pre-paid health plan population of over 5 million members nationally, a targeted intervention model has been developed to identify adolescents at high risk for becoming infected with the AIDS virus. METHOD: All health plan members age 12-21 who present for a health care visit by appointment are give
[AIDS in central africa: information systems, role of the media, and interface adolescents environment]: Int Conf AIDS 1989 Jun 4-9; 5:775 (abstract no. D.505); Kewa-Ilunga WB, Nyengele MN; Departement de l'Information et de la Presse, Lingwala, Kinshasa, Zaire
Stressful life events and social supports as mediators of safe behaviors among runaway and gay youths.: Int Conf AIDS 1989 Jun 4-9; 5:775 (abstract no. D.506); Haignere C, Rotheram-Borus M, Bradley J, Koopman C, Hardin M; HIV Center for Clinical and Behavioral Studies, New York State Psychiatric Institute and Columbia University, New York, NY, USA; To evaluate the effectiveness of stressful life events and social supports as mediators of safe sex behaviors among runaway and gay youths. A review of the literature, focus groups, and a chart review in community agencies were used to construct measures of stressful life events and social supports
Risk factors associated with a history of sexually transmitted disease among adolescents.: Int Conf AIDS 1989 Jun 4-9; 5:776 (abstract no. D.507); DiClemente R, Forrest K, Mickler S; University of California, San Francisco, California, USA; To identify a profile of risk factors associated with a history of treatment for sexually transmitted disease. A national sample of 1,129 college undergraduates, ages 18-23, completed self-administered questionnaires assessing knowledge, attitudes about AIDS, prevalence, type and frequency of sexual
A comparative analysis of risk behaviors among a school-based and juvenile detention facility sample of adolescents in San Francisco.: Int Conf AIDS 1989 Jun 4-9; 5:776 (abstract no. D.508); DiClemente R, DuNah R; University of California, San Francisco, California, USA; To describe a comparative risk behavior analysis of adolescents residing in a juvenile detention facility (YGC) and a school-based sample. Adolescents, ages 14-18, attending high schools in San Francisco (N=802) and incarcerated at a juvenile detention facility (YGC; N=113) completed identical self-
Evaluation of a targeted AIDS/HIV education program for secondary school students in a medium-size USA metropolitan area.: Int Conf AIDS 1989 Jun 4-9; 5:776 (abstract no. D.509); Copello AG, Sheets R, Ross S, Curvin M; Vanderbilt University, Nashville, Tennessee, USA; To evaluate change in AIDS/HIV knowledge level among secondary school students following a targeted education program. An education program was developed involving a minimum of two hours by trained health teachers. The curriculum stressed HIV modes of transmission and risk reduction measures. 500 st
Knowledge of HIV transmission and adolescent sexual behavior.: Int Conf AIDS 1989 Jun 4-9; 5:776 (abstract no. D.510); Ziffer J, Ziffer A, Bywater M, Bywater L; Rollins College, Winter Park, Florida 32789, USA; To assess how the knowledge of HIV transmission affects the sexual behavior of the adolescent. METHOD: A survey of 210 students, 109 men and 101 women in 9th to 12th grade, was completed. Knowledge of transmission of AIDS, sexual activity, change in sexual behavior, and source of educational information on H
Intervention model: working with gay and lesbian youth at risk for HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:776 (abstract no. D.511); Hunter J; Hetrick-Martin Institute, Inc. New York, New York, USA; To develop an effective, comprehensive intervention program for gay and lesbian youth who are at risk for HIV infection and train professionals to work with this population. METHOD: The methods used are focus groups for youth, workshops, and community forums. We have worked with over 1,500 youth in the last
Report of CWLA's committee on serving children and youth with HIV infection in residential group care.: Int Conf AIDS 1989 Jun 4-9; 5:776 (abstract no. D.512); Emery LJ, Anderson G, Anderson VM, Horowitz RM, Gitelson P, Annin JB; Child Welfare League of America, Washington, D.C. USA; To address issues of concern to Board members, executives, administrators, clinicians, child care staff and volunteers surrounding the care of children and youth who are emotionally disturbed, infected with HIV, and in need of therapeutic residential group care. METHOD: A subcommittee of CWLA s Task Force on
[Information and behavioral changes in the young population facing AIDS: role of mediators]: Int Conf AIDS 1989 Jun 4-9; 5:777 (abstract no. D.513); Estournel JL, Leveque D, Maurel H, Cordonnier JP; Administrateur MNEF, Paris
A nation-wide network of AIDS prevention for adolescents 18 month evaluation.: Int Conf AIDS 1989 Jun 4-9; 5:777 (abstract no. D.516); Jayle D, Richard H, Bunde-Birouste A, Guillemot D, Rayon D; APS: Association pour la Prevention du SIDA; Paris, France; 1) Design, implement, and evaluate a training program for AIDS prevention educators; 2) Create an interactive network of AIDS educators nation-wide. Following a pilot project implemented in the Paris metropolitan region, APS (Association pour la Prevention du SIDA) enlarged its methods and activiti
Outreach model for HIV positive adolescents and adolescents currently at high risk for HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:777 (abstract no. D.518); Hummel R, Rodriguez G, Brandon D, Wells D; New Jersey State Department of Health, Trenton, New Jersey, USA; Careful analysis of current indicators of risk behavior suggest that adolescents may be the next group in the U.S. to be hard hit by the AIDS epidemic. In an attempt to determine needs and characteristics of high risk adolescents in New Jersey, a pilot survey of 27 youths was conducted on the streets of Newa
From theory to practice: a culturally, linguistically and age-appropriate HIV risk reduction program for adolescents.: Int Conf AIDS 1989 Jun 4-9; 5:778 (abstract no. D.519); Stoller EJ, Gibson WP, Smyly V, Rojas de Colville E, Nestor S, Oliva GE; Department of Public Health, San Francisco, California, USA; To describe the Wedge : a four-session educational strategy for HIV risk reduction among San Francisco youth. Following six months of pilot testing, a four-session teaching model was launched in February of 1988. The model was designed to incorporate the strengths of existing education strategies: (
AIDS education in the workplace and the family.: Int Conf AIDS 1989 Jun 4-9; 5:778 (abstract no. D.523); Baldwin W, Cain VS; National Institute of Child Health and Human Development, NIH, Bethesda, Maryland, USA; To investigate the impact of AIDS education as provided in the workplace on the level of correct knowledge about AIDS especially those dimensions relating casual contact as might occur in the workplace. Also, such education may have a secondary effect if parents then educate their children. Therefore, a seco
The relationship between health status and psychological well-being among healthy men and men with ARC or AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:779 (abstract no. D.524); Schmidt RM, Castro FP; Center for Preventive Medicine and Health Research, San Francisco State University and Pacific Presbyterian Medical Center, San Francisco, California USA; To evaluate the relationship between health status and psychological well-being among healthy men, men with ARC and men with AIDS. Twenty-four homosexual men diagnosed with ARC and 22 homosexual men with AIDS were assessed for psychological well-being and compared with 24 healthy heterosexual male c
Personal AIDS-related behaviour among participants to the Stockholm IV International Conference on AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:779 (abstract no. D.526); Tirelli U, Mazzocco D, Serraino D, Lazzarin A, Clumeck N, Volberding P, Franceschi S; Centro di Riferimento Oncologico, Aviano, Italy; The present survey took advantage of the IV International Congress on AIDS to evaluate personal AIDS-related behaviour of health professionals from all over the world (68 countries). A brief questionnaire exploring a number of AIDS-related items (i.e. HIV-testing, current and past condom use, etc.),
[The condom: study of different types of messages in traditional symbols]: Int Conf AIDS 1989 Jun 4-9; 5:779 (abstract no. D.527); Troussier T; B.P. 1232 97494 St Denis Cedex (La R-eunion) France
Sexual decision-making among gay/bisexual men in a low incidence area for AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:779 (abstract no. D.528); Beeker C, Rose T; NYS AIDS Institute, Albany, New York, USA; To examine sexual decision-making under conditions of ambiguous risk among gay/bisexual men in a low-incidence area for AIDS. METHOD: Participants in a CDC-funded AIDS prevention study were asked to estimate their likelihood of HIV exposure in twelve sexual scenarios. The scenarios, based on anecdotal accoun
Using structured sexual diaries data to estimate rates, predictors and contexts of high-risk sexual behaviour.: Int Conf AIDS 1989 Jun 4-9; 5:779 (abstract no. D.529); Coxon AP, Davies PM; University of Wales, Cardiff UK; To assess the effects of Age and Type of relationship on the incidence of sexual behaviour of homosexual men in the United Kingdom , especially on the contexts in which high-risk behaviour occurs. Analysis of 3950 structured sessions from sexual diaries kept for a month by a volunteer sample of 170
Talking about sex.: Int Conf AIDS 1989 Jun 4-9; 5:779 (abstract no. D.530); Spencer L, Wellings K, Faulkner A, Keegan J; Social and Community Planning Research, London, England; Because sexual matters are rarely discussed publicly, little is known about the language people use to describe their own experience. The need for data to guide and inform the design of a questionnaire for use in the British Survey of Sexual Attitudes and Lifestyles has provided an opportunity to explore the
Changes in sexual behaviour of gay men under the impact of AIDS. II.: Int Conf AIDS 1989 Jun 4-9; 5:780 (abstract no. D.531); Bochow M, Markert S; INTERSOFIA, Berlin (West), Federal Republic of Germany; To analyze whether further changes in sexual behaviour of gay men are still taking place and whether those already identified in a previous survey (1987) have proved to be stable. A questionnaire of 62 questions on different topics relating to sexual behaviour and lifestyle of gay men was circulated
Manifestations of AIDSphobia at an outpatient care unit in Rio De Janeiro - Brazil.: Int Conf AIDS 1989 Jun 4-9; 5:780 (abstract no. D.532); Longo PH, Carvalho Neto LD, Sion FS, Morais de Sa CA; Gaffree & Guinle University Hospital, University of Rio de Janeiro (UNI-RIO) - Brazil; To study the etiology and incidence of somatic manifestations of HIV negative individuals concerned about AIDS who came to the AIDS Outpatient Care Unit at our Hospital presenting paranoid states and phobias related to AIDS. The study comprised 23 HIV negative individuals (17,04%) with AIDSphobia se
Immune modulation following the death of a cage-cohort: an animal model of psychological stress in AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:780 (abstract no. D.533); Sei Y, Skolnick P, Arora PK; Laboratory of Neuroscience, NIDDK, National Institutes of Health, Bethesda, MD, USA; The psychological stress, experienced by AIDS patients following the death of their sexual partners or close friends, has been suggested to influence immune functions. An attempt was made in these studies to establish an animal model for this type of psychological stress. We examined immune functio
Behaviour modification strategy in AIDS prevention.: Int Conf AIDS 1989 Jun 4-9; 5:780 (abstract no. D.535); Wynendaele B, Goede H; AIDS Task Force, E.E.C., Brussels, Belgium; Report on an experiment in Surinam and a discussion on methodology. To promote participative training models for health educators involved in AIDS prevention directed at behavioural change for specific target groups. To determine the most effective method for each objective and target group. METHOD: Theoreti
The use of three biopsychosocial interventions on the stress levels and immune functioning of HIV positive individuals.: Int Conf AIDS 1989 Jun 4-9; 5:780 (abstract no. D.536); Hamilton BC, Dufault E; Bellevue Hospital/Community Health Project., NY, NY USA; On the premise that personality coping styles are a co-factor in disease prognosis, we are assessing the effects of 3 biopsychosocial interventions on the stress levels and immune functioning of HIV+ patients. 10 seropositive patients underwent an intensive 16-week intervention involving Bioenergeti
Maintaining followup in prospective epidemiologic studies of HIV infection: experience in the Baltimore MACS study.: Int Conf AIDS 1989 Jun 4-9; 5:781 (abstract no. D.537); Fox R, Jones LT; The Johns Hopkins School of Hygiene and Public Health, Baltimore, MD, USA; To describe methods of maintaining participation in the Baltimore MACS, a volunteer study of the natural history of HIV in gay men. During a sample 3 month period, methods used to remind study participants (Ps) of their followup (FU) appointments, and return rates for each method were assessed. FU r
The Minnesota General Public AIDS Survey: a behavioral risk factor survey supplement.: Int Conf AIDS 1989 Jun 4-9; 5:781 (abstract no. D.538); Heckert K, Shultz J, Salem N; Minnesota Department of Health (MDH), Minneapolis, MN, USA; To assess AIDS-related knowledge, attitudes and behavior (KAB) among the Minnesota general public over time, using systematic sampling procedures of the CDC Behavioral Risk Factor Surveillance System (BRFSS). During September-December, 1986-1988, the MDH conducted the Minnesota General Public AIDS S
Trust: prerequisite for gay health.: Int Conf AIDS 1989 Jun 4-9; 5:781 (abstract no. D.539); Getty G, Stern P; University of New Brunswick, Fredericton, N.B. Canada; To explore the perceptions of gay men regarding; a) the problems they encounter while maintaining their health; and b) their views on how these problems can be solved. Data was gathered from a) unstructured interviews with 34 healthy gay men in the Maritimes; b) a log maintained while nursing people
How an AIDS patient manages his life - a study of seropositives to HIV patients in Sao Paulo city.: Int Conf AIDS 1989 Jun 4-9; 5:781 (abstract no. D.540); Teixeira P, Laurindo L, Gianna M, Kalichman A, Grangeiro A, Laurindo J, Munhoz R; AIDS-Reference and Training Center-Health Secretariat of Sao Paulo-Brasil; For the enormity of cases in Sao Paulo city, 3213 until December, 1988, we think that a study trying to understand more about the AIDS patient s life is urgent. GENERAL -1) To provide subsidies in the sense of to under- stand and deal better with the disease; 2) To propose support measures for the patients
AIDS behavior risks in women of reproductive ages in a family planning clinic.: Int Conf AIDS 1989 Jun 4-9; 5:781 (abstract no. D.541); Fast JE; Baylor College of Medicine Population Program, Houston, Texas, USA; As a corollary to an United States Centers for Disease Control seroprevalence study on women of reproductive ages 17-44, a survey was conducted between October, 1987 and November, 1988 on 438 mixed ethnic origin women (31% Black, 40% Hispanic, 27% White) entering into the Baylor Population Program Family Planning Clini
Pilot study of knowledge, attitude, opinion and behaviour of Lagosian Nigerians about HIV infections and AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:781 (abstract no. D.542); Somorin AO, Lewis I, Odunowo B, Hunponu-Wusu OO, Somorin JE; College of Medicine, University of Lagos, Nigeria; To determine indices of Knowledge and Attitude of Lagosian residents using their Beliefs and Opinion, about contracting and preventing spread of AIDS in Nigeria . METHODOLOGY: The study was carried out by interviewers using specially prepared coded Questionnaire Forms to screen 239 healthy looking Nigerians
Occupational therapy actuation at a multidisciplinary team turned to AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:782 (abstract no. D.543); Moreira CE, Tannus LM, Lima MP, Bellucci SB; Centro Corsini. (CCII). Campinas. Sao Paulo. Brazil; CCII is a philanthropic non-governmental entity, multidisciplinary compound by: doctors, psychologists, nurses, dentists, physical education teachers and occupational therapists. Research based on individual attendance of HIV infected clientele from CCII, ambulatorial, hospitable, domiciliar. The clientele belongs to d
Towards improving condom use in a sexually transmitted disease clinic.: Int Conf AIDS 1989 Jun 4-9; 5:782 (abstract no. D.544); Beidas S, Byrnes D; Saint Michael's Medical Center, Newark, N.J. USA; To assess patient knowledge about condoms and spermicidals in a high risk setting for STD and HIV infection. Twenty four patients (22 men, 2 women), mean age 25.8+/-5.7(SD), were consecutively interviewed at an Inner City STD Clinic. Their knowledge of condoms and spermicidals was evaluated through
The British survey of sexual attitudes and lifestyles - a pilot study.: Int Conf AIDS 1989 Jun 4-9; 5:782 (abstract no. D.545); Field EJ, Johnson AM, Wellings K, Wadsworth J, Bradshaw SA, Anderson RM; SCPR, London, UK; To assess the feasibility of a major national study of sexual attitudes and lifestyles in a random sample of the British population. METHOD: A stratified multi-stage sample of addresses was taken from the Postcode Address File and one person aged between 16 and 59 randomly selected from each address. Respond
The HIV support group: an analysis using different social problem models and different institutional settings.: Int Conf AIDS 1989 Jun 4-9; 5:782 (abstract no. D.546); Nessel JT; Bronx AIDS Community Service Project, Director of Education, N.Y.C. USA; To analyse HIV Support Groups in relationship to five social problem models: medical, social disorganization, value conflict, deviant behavior and labelling. To analyse HIV Support Groups at five types of institutions: residential drug treatment communities, methadone treatment programs, the criminal justice
Sexual behavior and its change after having knowledge about AIDS among Brazilian university students.: Int Conf AIDS 1989 Jun 4-9; 5:782 (abstract no. D.547); Diaz MM, Aoki FH, Goodson P, Diaz J, Vera S, Faundes A; CEMICAMP, Campinas, SP, Brazil; To assess the incidence of sexual behaviors that constitute risk of contracting AIDS among Brazilian University Students and the impact of information about AIDS on this incidence. A structured questionnaire on sexual behavior and knowledge about AIDS was mailed to 2609 students of the first two gra
A three component AIDS attitude scale.: Int Conf AIDS 1989 Jun 4-9; 5:782 (abstract no. D.548); Torabi MR, Yarber WL; Indiana University, Bloomington, Indiana, USA; To plan, implement, evaluate and study AIDS education programs, development of a valid knowledge test and attitude scale is essential. The purpose of this study was to develop a valid, Likert-type scale to assess student attitudes towards AIDS and AIDS prevention. The three-component attitude model, consisti
A method for counselling patients with neurological problems associated with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:783 (abstract no. D.549); Bor R, Miller R, Willison J; Royal Free Hospital and School of Medicine, London, England; To describe a method for counselling patients, family members, partners and health care staff for neurological problems at different stages of illness, associated with AIDS. The Milan Approach in family therapy was applied. Interventions by the counsellor, who may be a doctor, nurse or other member
Ervolino counselling/AIDS programme.: Int Conf AIDS 1989 Jun 4-9; 5:783 (abstract no. D.550); Erovlino LA, Ervolino Neto J, Carvalho Silva A, Torres MC; Health Ministry, Brasilia, DF Brazil; To improve the prevention level in HIV transmission. The Ervolino Counselling/AIDS programme is a Human Resources training process. It uses self analysis to obtain an adequate balance of the individual emotions, which is viewed as a key factor for an effective change to a preventive behavior. Role
The integration of safer sexual practices and verification of medical diagnosis in psycho-social intervention at the community level.: Int Conf AIDS 1989 Jun 4-9; 5:783 (abstract no. D.551); Ryan WJ; Director of Support Services, Comite sida aide Montreal. Canada; - To describe psycho-social interventions with People With AIDS or persons who are seropositive concerning safer sexual practices and verification of information concerning medical diagnosis. Literature has shown that both of these components have been underemployed in interventions at the point of diagnosis
Training of institutional professionals as HIV infection/AIDS prevention counseling trainers in Peru.: Int Conf AIDS 1989 Jun 4-9; 5:783 (abstract no. D.552); Bossio L, Caceres C, Goodfriend N, Carovano K; Programa VIDA, Lima, Peru; Developing a short program for training a multidisciplinary group of professionals, coming from several institutions, as trainers in HIV infection/AIDS prevention counseling, and leading them in the design of a working strategy within their institutions. Thirty Peruvian institutions, including Natio
Counselling workshop for health professionals.: Int Conf AIDS 1989 Jun 4-9; 5:783 (abstract no. D.553); Grondin M, Hulin M; Department of Public Health, City of Toronto, Toronto, Ontario, Canada; HIV illness was becoming an issue for health care providers working for Public Health Departments. This workshop was instituted to provide Public Health Nurses with the tools to develop counselling skills re: HIV illness. METHOD: A two day workshop for Public Health professionals involved in HIV counselling
A comprehensive program to support individuals infected with HIV.: Int Conf AIDS 1989 Jun 4-9; 5:783 (abstract no. D.554); Williams JL, Magruder CD, Sanchez JL, Quattromani FL; Womack Army Community Hospital, Fort Bragg, NC, USA; To describe and evaluate a multi-disciplinary program designed to support people who have been infected with HIV. The U.S. Government has a formal policy which states that all individuals on active duty status in the Armed Forces must have an HIV antibody test at least biennially. When an ELISA test
A behavioral approach to HIV prevention among sero-negative or untested gay/bisexual men with a history of unsafe behavior.: Int Conf AIDS 1989 Jun 4-9; 5:784 (abstract no. D.555); Swarthout D, Gonsiorek J, Simpson M, Henry K; Hennepin County Red Door Clinic, Minneapolis, Minnesota, USA; To reduce the incidence of unsafe sexual behavior among gay/bisexual men who have either tested HIV antibody negative or whose serostatus is unknown. METHOD: A pilot program was conducted from 2/88 - 12/88 focusing on individuals who have a history of unsafe sexual behavior within the past year. Program goal
Workshops for parents of HIV antibody positive children.: Int Conf AIDS 1989 Jun 4-9; 5:784 (abstract no. D.556); Ross J, Rosonakis J; Haemophilia Foundation of Australia; The needs and concerns of parents of children with haemophilia and HIV are extraordinary, and appear to be greater than those of the children themselves. Many stem from the parents isolation, both emotional and geographic (in Australia ). Following requests the Haemophilia Foundation of Australia applied for
Implementing AIDS prevention counseling services at an STD clinic.: Int Conf AIDS 1989 Jun 4-9; 5:784 (abstract no. D.557); Gibson PM, Gibson WP, Bolan G; Department of Public Health, San Francisco, CA, USA; To integrate AIDS prevention services into the treatment of patients at an STD clinic. An organizational approach was taken in developing AIDS prevention services. An HIV Task Force was formed, staff and patients were surveyed and a standardized protocol was designed. All professional health care st
The use of hypnosis with asymptomatic HIV infected people.: Int Conf AIDS 1989 Jun 4-9; 5:784 (abstract no. D.558); Gochros JS; HIV Unit, Tripler Army Medical Center, Honolulu, Hawaii, USA; To explore the use of hypnosis in assisting asymptomatic HIV infected people cope with stress and anxiety. METHOD: This paper is based on the author s use of hypnosis with men she is seeing in individual therapy, the members of an HIV+ support group she facilitates, and on the feedback she has received. RESU
Why volunteers in a welfare state?: Int Conf AIDS 1989 Jun 4-9; 5:784 (abstract no. D.559); Madsen K, Kristensen JH; AIDS-linien, Copenhagen, Denmark; Denmark is a so-called welfare state. We live in the economically privileged part of the world. We are known for taking care of people from cradle to grave . We expect paid professionals to take care of our health and social problems. This means that we have a large staff of public servants. With this in mind it is u
Ankali project: client evaluation of a program providing volunteer emotional support for people with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:784 (abstract no. D.560); McCallum L, Dykes J, Watts J, Buhrich N, Turbitt P; Albion Street (AIDS) Centre, Sydney Hospital, Sydney, Australia; To evaluate the effectiveness of a volunteer emotional support program for people with AIDS from the clients perspective. METHOD: Clients who had been matched with a volunteer for at least three months were interviewed. An interview schedule was designed to collect information about the quality and quantity
A formula for assessment of individual risk activity for HIV infection with modification for behavior.: Int Conf AIDS 1989 Jun 4-9; 5:785 (abstract no. D.561); DeBernardo RL; Yale University, New Haven, CT., USA; To develop a method capable of assigning a probability figure to an individual s risk of contracting AIDS, given his or her individual risk factors and preventive measures. Numerous reports of clinical and epidemiological studies were analysed to determine the lowest common denominators for risk fac
A training program for individuals conducting counseling and testing.: Int Conf AIDS 1989 Jun 4-9; 5:785 (abstract no. D.562); Rosenthal S, Fowley C, Solomon K, James R, Rautenberg E, Schultz S; New York City Dep't. of Health, New York, N.Y. AIDS Program Services; To provide nurses, drug counselors, public health educators, social workers, physicians and other health and social service professionals with the skills needed for advising patients/clients about AIDS and conducting counseling related to HIV antibody testing. METHOD: A counseling and testing training progra
Art therapy with HIV positive patients, mourning, restitution and meaning.: Int Conf AIDS 1989 Jun 4-9; 5:785 (abstract no. D.563); Fenster GM; Art Therapy Section, Walter Reed Army Medical Center, and the Walter Reed Retrovirus Research Group, Washington, D.C., United States of America; To present the importance and value of creative, expressive activities with HIV positive persons. Didactic presentation and 20 minute video. The videotape features approximately 150 slides of art productions (by HIV positive military personnel) synchronized to 9 musical selections which match the pr
Utilizing a family intervention model to prevent AIDS worker burnout with inner city patients.: Int Conf AIDS 1989 Jun 4-9; 5:785 (abstract no. D.564); Walker G; The Ackerman Institute for Family Therapy, New York, New York, USA; In many large cities the majority of all new AIDS diagnoses are found in Inner City families. These families present as drug using, multi-problem families, most of whom sustain more than one loss from HIV infection. The complexity of family problems, the stigmatization of AIDS, and the overburdening of AIDS workers neg
HIV positive support groups - a model of intervention.: Int Conf AIDS 1989 Jun 4-9; 5:785 (abstract no. D.565); Solomon K, Rosenthal S, James R, Santiago D, McLoughlin S; NYC Department of Health, New York, N.Y. AIDS Program Services; 1. To provide group support for individuals who have tested HIV positive and their partners. 2. To provide barrier free group services to assist those who have tested positive more effectively cope physically, emotionally, and socially. METHOD: The Department of Health provides professionally facilitated gr
Bereavement issues for survivors of persons with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:785 (abstract no. D.566); Colburn KA, Malena D; Hospice of Central Iowa, Des Moines, Iowa, USA; To determine if survivors of persons who died of AIDS have a different social and emotional environment in resolving grief. METHOD: Personal interview which surveyed what survivors were doing to handle their grief, what resources they had in the community and if they were accomplishing the tasks of grief. RE
Integrated identity through the emotional support approach.: Int Conf AIDS 1989 Jun 4-9; 5:786 (abstract no. D.568); Pelfini A; Program Coordinator: Emotional Support Program. Shanti Project, San Francisco, California, USA; To substantiate the impact of Emotional Support on identity integration for individuals diagnosed with AIDS. One on one peer counseling contributes greatly to the emotional health of individuals facing an AIDS diagnosis. This diagnosis brings about a dual identity conflict in being an individual and
[Responding to the health care needs of seropositives and patients: role of a multi-partner association]: Int Conf AIDS 1989 Jun 4-9; 5:786 (abstract no. D.569); Leveque D, Maurel H, Cordonnier JP, Estournel JL; President de la mnef, Paris France
Information group for people with HIV infection or AIDS and their partners.: Int Conf AIDS 1989 Jun 4-9; 5:786 (abstract no. D.571); Hedge B, Glover L; St. Mary's Hospital, London, England; Many people with HIV infection or AIDS and their partners find it difficult to share their fears and feelings and so lack crucial social support. Such people often find it difficult to join support groups but are keen to learn more about HIV. It was proposed to use an information group to also provide social
Training in HIV/AIDS counselling.: Int Conf AIDS 1989 Jun 4-9; 5:787 (abstract no. D.573); McCreaner A, Green J; National Aids Counselling Training Unit (NACTU), St. Charles' Hospital, Exmoor Street, London, UK; To set out the key elements in training counsellors for HIV/AIDS work. METHOD: NACTU has trained over 5,000 workers from the UK and abroad in HIV/AIDS counselling. Based on experience and evaluation of each course run - a training format has been arrived at. A two-tier training approach has been ado
The ultimate(?) challenge of counselling - The Noah's Ark-Red Cross Model.: Int Conf AIDS 1989 Jun 4-9; 5:787 (abstract no. D.574); Morfeldt JO; Noah's Ark-Red Cross Foundation, Stockholm, Sweden; This presentation will briefly describe the ongoing challenge at our centre in Stockholm. Humanity, solidarity, knowledge, understanding, behavioural modification are key words in our daily work. The ultimate(?) challenge of counselling is built on our own and WHO s ideas that complete counselling can only be achieved
[Counseling: a strategy for information in french speaking African countries]: Int Conf AIDS 1989 Jun 4-9; 5:787 (abstract no. D.575); Lebas J, M'Pele P, O'Dell V, Aeberhard F, Carballo M; Medecins du Monde. Paris. France
A support group model for counselling couples affected by AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:787 (abstract no. D.576); Stone J, Pawlowski W; Johns Hopkins University/AIDSCOM and Whiteman-Walker Clinic Support Group Facilitator, Washington, D.C. USA; The AIDS epidemic forces couples impacted by the disease to face new issues in their relationships. In turn, therapists and counsellors around the world are forced to re-examine roles and relationships in providing therapy and support. The authors examine issues which couples have faced during the three and
Group psychotherapy with HIV+/drug users.: Int Conf AIDS 1989 Jun 4-9; 5:787 (abstract no. D.577); Upadhya G, Rosen FM; Bronx-Lebanon Hospital/Albert Einstein College of Medicine Bronx, New York 10456 USA; To describe the efficacy of group psychotherapy in caring for people with AIDS and HIV infection who are socioeconomically disadvantaged IV-drug users living in an inner city community of New York. The support group met weekly, led by a psychiatrist and a psychologist facilitating the members to art
A prospective quality of life study in patients with HIV infections.: Int Conf AIDS 1989 Jun 4-9; 5:787 (abstract no. D.578); Geels RM, Vrehen H, Bonsel G, Jager JC, Borleffs JC; University Hospital Utrecht, The Netherlands; AIDS and other HIV infections are expected to have a serious impact on Quality of Life (QOL) of HIV infected patients; however, till now this has never been documented. In order to test this hypothesis, a method for monitoring and describing QOL for these patients has been developed 1) to measure QOL of HIV infected pa
Evaluating psychoeducational interventions in the HIV seropositive population.: Int Conf AIDS 1989 Jun 4-9; 5:788 (abstract no. D.579); Landers S, Keefe C, Gross M, Kunches L; MA Department of Public Health, Boston, MA, USA; To summarize evaluation strategies for a 3-session psychoeducational group for persons testing HIV antibody positive. The group serves many purposes including the provision of (1) information about medical, mental health, treatment protocols, and other options for health maintenance, (2) informatio
The risks of abstinence: sexual decision making in the ERA of AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:788 (abstract no. D.580); Gochros HL; School of Social Work, University of Hawaii, Honolulu, Hawaii, USA; To consider the ramifications of the advocacy of sexual abstinence in response to the threat of AIDS. The author is a university based sex educator and counselor in addition to his work as president of the AIDS Foundation of Hawaii. This paper represents observations from his clinical and educationa
Issues in AIDS training for drug treatment personnel.: Int Conf AIDS 1989 Jun 4-9; 5:788 (abstract no. D.582); Ashery RS; National Institute on Drug Abuse, Rockville, Maryland, USA; To present issues and solutions for AIDS training for staff in drug treatment programs. By anecdotal information gathered through the development of a systematic and comprehensive AIDS training program for personnel in drug treatment programs in the United States . The complexities of AIDS,
AIDS information campaign has significantly reduced the incidence of acute type B hepatitis in an endemic area.: Int Conf AIDS 1989 Jun 4-9; 5:788 (abstract no. D.583); Bortolotti F, Cadrobbi P, Crivellaro C, Carretta M, Francavilla E, Noventa F; Clinica Medica 2, University, Padua. Italy; To evaluate whether AIDS information campaign has reduced the incidence of acute hepatitis B , which is transmitted by the same routes as AIDS. In 1978 a prospective study of acute hepatitis was started in our Department of Infectious Diseases, which is a referral center for an average 81% of cases
Reliability of data collected on sexual histories in Haitian immigrants in Montreal.: Int Conf AIDS 1989 Jun 4-9; 5:780 (abstract no. D.584); Adrien A, Tousignant Y; Community Health Department, Montreal General Hospital and McGill University, Quebec, Canada; To evaluate test-retest reliability of a questionnaire on sexual histories administered to Montrealers of Haitian origin. Test-retest reliability of the questionnaire was assessed using 30 subjects (13f., 17m.) aged 15-39. The median of the interval between the two questionnaire administrations was
"UNIK". A project employing former IVDA'S in HIV preventive and support work.: Int Conf AIDS 1989 Jun 4-9; 5:788 (abstract no. D.585); Engh M, Ringdal A, Nestande P, Lien A, Saatvedt J, Jensen F; Dep. of Aids Prevention, Oslo City Health and Environment Department, Norway; There is a need for new approaches in the hiv preventive and supportive work among drug users. This project, established by the Department of Aids Prevention in May 1987, employs, on a full time basis and with normal wages, both social workers and former IVDA s. The aim is to work out new methods utilizing
A comprehensive AIDS program in a methadone treatment program.: Int Conf AIDS 1989 Jun 4-9; 5:789 (abstract no. D.586); Foss BM, Connolly G, Cassidy P; New Bedford Area Center for Human Services, Inc., New Bedford, Massachusetts, United States of America; Our presentation will consist of an explanation of our methadone program and its six core components related to HIV infection. The components to be discussed are: AIDS education, HIV antibody testing, client advocacy, psychotherapy, and community and street outreach. AIDS education is mandatory to all clients in our me
Cocaine use during pregnancy: validity and associations with HIV risk behaviors.: Int Conf AIDS 1989 Jun 4-9; 5:789 (abstract no. D.587); Sackoff J, Kline J; HIV Center for Clinical and Behavioral Studies, NYS Psychiatric Institute and Sergievsky Center, Columbia University, New York, N.Y., USA; To assess the validity of interview data on cocaine use during pregnancy, and associations between cocaine use and risk behaviors for HIV infection. METHOD: Over 150 women who delivered at a New York City hospital without receiving prenatal care completed a brief structured interview about their use of intra
Observational and narrative data on patterns of intravenous drug use as related to HIV-1 infection.: Int Conf AIDS 1989 Jun 4-9; 5:789 (abstract no. D.588); Page JB, Chitwood DD, Smith PC, Kane N, Inciardi J, McBride DC, Clyde BM; University of Miami School of Medicine, USA; To demonstrate the utility of data collected in the natural habitats of drug users for constructing intervention strategies. In a longitudinal study of 230 street-recruited intravenous drug users (IVDUs) in Miami, Florida, the investigators include direct observation of needle using behaviors and el
Demographics, drug use and sexual behavior of intravenous drug users (IVDU's) with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:789 (abstract no. D.589); Schrager LK, Friedland GH, Kahl P, Feiner C, Klein RS; Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA; Define demography, drug and sex behavior of IVDU s with AIDS. Detailed standardized interview of hospitalized AIDS patients. 112 hospitalized IVDU s with AIDS participated from 10/84-2/88. Demography: male 72%, female 28%; Hispanic 49%, black 33%, white 14%; Bronx residence 86%; mean age 35
Self-reported need for treatment among IV drug abusers in an HIV-clinic.: Int Conf AIDS 1989 Jun 4-9; 5:789 (abstract no. D.590); Toraker P, Pehrson PO, Spangberg L; Karolinska Institute, Department of Psychiatry, Huddinge, Sweden; The aim of the study was to ascertain whether there is a self-experienced need for treatment of drug abuse at a HIV-clinic. A questionnaire was filled out according to the responses of the patients. Patients were asked about present abuse, present treatment and perceived need for treatment.
A major intervention research project among parenteral drug users not in treatment in Chicago, Illinois: Int Conf AIDS 1989 Jun 4-9; 5:790 (abstract no. D.591); Horan HJ, Wiebel WW, Chene DG; School of Public Health, University of Illinois at Chicago, IL, USA; Describe the effects of attempts to change drug and sexual behavior among this population, and differentiate among approaches attempted. HIV testing; use of a national face-to-face interview; ethnographic fieldwork; public health outreach worker intervention. HIV status has varied considera
Needle sharing behaviors and HIV serostatus in Miami, Florida.: Int Conf AIDS 1989 Jun 4-9; 5:790 (abstract no. D.592); Inciardi JA, Chitwood D, McCoy CB, McBride DC; University of Delaware, Newark, DE, USA; To understand the relationships between the extent of needle sharing by IV drug users and their rates of seropositivity. METHOD: As part of an AIDS prevention/intervention project funded by the National Institute on Drug Abuse, 500 IV drug users were recruited from several neighborhoods in Miami, Florida whe
Does methadone program withdrawal (M.P.W.) result in an increase of crime, overdose accidents and HIV transmission among I.V. drug users (IVDU).: Int Conf AIDS 1989 Jun 4-9; 5:790 (abstract no. D.594); Mezzelani P, Lugoboni F, Venturini L, Bricolo R; Istituto di Patologia Speciale Medica - University of Verona - Italy; In Italy 65% of all cases of AIDS are among IVDU. Italian experience shows the failure of methadone maintenance programs; therapeutic communities and naltrexone detoxification programs are promoted instead. This paper s aim is to ascertain whether M.P.W. results in an increase of crime, overdose accidents, a
[Evaluation of fear of AIDS in heroin addicts]: Int Conf AIDS 1989 Jun 4-9; 5:790 (abstract no. D.595); Salto C, Peri JM, Bayes R; Spain
Prevention of HIV-infection in a methadone maintenance program.: Int Conf AIDS 1989 Jun 4-9; 5:790 (abstract no. D.596); Huber F, Haas H; Department of Social Psychiatry University Hospital Zurich, Switzerland; Can a methadone maintenance program prevent HIV-neg. IV drug abusers from getting infected? In a retrospective study 21 IV drug abusers were again tested and the therapists were asked about the risk behaviour and social integration of their patients. We were able to show for 21 patients who
AIDS and I.V. drug use in Puerto Rico.: Int Conf AIDS 1989 Jun 4-9; 5:791 (abstract no. D.597); Iramain CA; Drug Abuse Fdn. PR, P.O. Box 972, Saint Just, PR 00750 (USA); As of 1/17/89, 2055 AIDS total cases (83 % males/17 % females) were officially reported in the Commonwealth of Puerto Rico , USA [623 cases/million]. Most important groups were: 1) heterosex. IVDUs: 1120 cases (56 %); 2) homo/bi men: 402 (20 %); & 3) homo/bi men IVDUs: 257 (13 %). So, 1377 (69 %) of our adult/adol
The adjunctive role of addict directed support groups in the care of HIV infected addicts.: Int Conf AIDS 1989 Jun 4-9; 5:791 (abstract no. D.598); Comella B; Project TRUST, Boston City Hospital, Boston, MA, USA; To assess the contribution of motivational strategies and mutual support groups for the psychological, medical, and addiction related care of HIV infected addicts. Facilitator directed support groups, emphasizing AIDS related concerns, sexual, physical, and emotional responses to HIV disease. Focuse
Nonvoluntary treatment of intravenous drug abusers to curb the spread of HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:791 (abstract no. D.599); Leukefeld C, Battjes R, Pickens R; National Institute on Drug Abuse, Rockville, MD, USA; To explore nonvoluntary drug abuse treatment under Criminal Justice System sanction (i.e. probation, parole or civil commitment) to reduce the spread of HIV among intravenous drug abusers (IVDAs). The most recent research findings and the input of a convened group of expert consultants were used to
Treatment modalities for pregnant drug dependent women as an effort to prevent perinatal AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:791 (abstract no. D.600); Finnegan LP, Kaltenbach K, Weiner S, Wapner R; Thomas Jefferson University, Philadelphia, Pennsylvania, USA; The interface between AIDS and drugs is a key issue from the standpoint of perinatal transmission. Unfortunately, these women, who are already weakened by unemployment, illiteracy, homelessness, crime and other diseases, including their alcoholism and drug addiction, are now those at risk for the human immunodeficiency
[Syringe exchange and sexual life of IV drug abusers]: Int Conf AIDS 1989 Jun 4-9; 5:791 (abstract no. D.601); Espinoza P, Polo Devoto J, Bouchard I, Lebouc V, D'Hallivilee P; EHPNF Allee des Thuyas 94261 Fresnes, Cedex, France
Relationship between social support and loneliness and hospital service-seeking in intravenous drug-using males.: Int Conf AIDS 1989 Jun 4-9; 5:791 (abstract no. D.602); Hoffman X, Winiarski M; Albert Einstein College of Medicine/Yeshiva University, Bronx, N.Y., USA; Psychoneuroimmunologic investigations demonstrate an integration between psychosocial response and immune function. This retrospective correlational study explores relationships between availability and adequacy of social support and objective loneliness on the seeking of hospital services among intravenous
Research with intravenous drug users. Problems and practical recommendations.: Int Conf AIDS 1989 Jun 4-9; 5:792 (abstract no. D.603); Carballo-Dieguez A, El-Sadr W, Gorman J, Joseph M, McKinnon J, Sorell S; HIV Center for Clinical and Behavioral Studies, New York State Psychiatric Institute and Columbia University, New York City, NY, USA; To successfully recruit 300 IVDUs to participate as subjects in a 5 year study on natural progression of HIV disease. METHOD: Several methods of recruitment were utilized: a poster campaign, informational meetings at methadone clinics and inpatient wards, and $10.-/hour payment. Payment was the main
Profile of users of the drug IV in the periphery of Campinas.: Int Conf AIDS 1989 Jun 4-9; 5:792 (abstract no. D.604); Lima MP, Rocha MD, Tanibata PR, Mielle A, Ferreira RP, Lorenzetti TM; Hospital e Maternidade Celso Pierro da Pontificia Universidade Catolica de Campinas- S.P. Brasil; The University Hospital of PUCCAMP attends a region of 1 million inhabitants. It is localized between various districts, the majority, without any infrastructure (water, sewer, health), which characterizes it as the area most in need of the municipality. Proceeding from that region a study was made of a group of 62 dru
Perception of health and AIDS risk behaviors among IV drug users.: Int Conf AIDS 1989 Jun 4-9; 5:792 (abstract no. D.605); Trapido EJ, McCoy CB, Chitwood DD, McCoy HV; University of Miami Medical School, Miami, FL, USA; To relate the perception of health among a group of IV drug users to AIDS risk behaviors and indicators. As part of a study which tests two interventions to reduce the risk of AIDS among IVDUs, 346 subjects were recruited from drug networks, tested for HIV-1 positivity, and interviewed. Bivariate an
Promotion of HIV prevention activities in drug treatment centers in the United States.: Int Conf AIDS 1989 Jun 4-9; 5:792 (abstract no. D.606); Jones TS, Gardom JC, Poppe PO, Conlon R, Bowen GS; Centers for Disease Control, Atlanta, USA; To implement on-site HIV prevention programs for clients and their sex partners in drug treatment centers (DTCs) in the 24 states and cities with the largest number of AIDS cases in the United States . HIV prevention in DTCs has been a major priority for the Centers for Disease Control (CDC) in fisc
Behavioural changes for HIV risk-reduction in two cohorts of intravenous drug users (IVDUs) in New York City.: Int Conf AIDS 1989 Jun 4-9; 5:792 (abstract no. D.607); Chu A, Brown LS, Banks S, Nemoto T, Primm BJ; Addiction Research and Treatment Corporation, Brooklyn, NY USA; To assess those behavioural changes that are a response to the high risk for HIV infection experienced by IVDUs. During 1987 and 1988, 218 and 223 subjects respectively were recruited from methadone clinics in NYC. Following informed consent, a standardized questionnaire was administered pertaining
HIV seroprevalence in heroin users: a demographic and behavioural analysis.: Int Conf AIDS 1989 Jun 4-9; 5:792 (abstract no. D.608); Banks S, Brown LS, Chu A, Nemoto T, Valdes M, Primm BJ; Addiction Research and Treatment Corporation, Brooklyn, NY USA; To examine the association of demographic factors and heroin use patterns on the HIV serostatus of IVDUs. Following informed consent, a questionnaire of demographics and drug behaviours was administered to patients in NYC drug treatment clinics in 1987. Of the 218 subjects, 7.8% were White; 45% Blac
Needle-sharing patterns as a predictor of HIV seroprevalence among New York City intravenous drug users (IVDUs).: Int Conf AIDS 1989 Jun 4-9; 5:793 (abstract no. D.609); Yee K, Brown LS, Primm BJ, Nemoto T, Foster K; Addiction Research and Treatment Corp., New York City, USA; To examine the association between patterns of needle-sharing and HIV seroprevalence among IVDU s in NYC. Following informed consent, 218 patients, recruited from methadone maintenance clinics in Brooklyn and Manhattan, were administered a standardized questionnaire in 1987. This sample included 45%
Patterns of cocaine use and its association to HIV seroprevalence among intravenous drug users (IVDUs).: Int Conf AIDS 1989 Jun 4-9; 5:793 (abstract no. D.610); Nemoto T, Brown LS, Primm BJ, Chu A, Foster K, Ajuluchukwu D; Addiction Research and Treatment Corporation, New York City, USA; To investigate patterns of cocaine use and its association to HIV infection among IVDUs. In 1987, a total 218 participants: average age=33 years, 36% males and 64% females, and 45% Blacks, 47% Hispanics, and 8% Whites, were recruited from methadone clinics in Manhattan and Brooklyn, New York City. A
Behavioral risk factors of human immunodeficiency virus (HIV) infection among intravenous drug users (IVDUs).: Int Conf AIDS 1989 Jun 4-9; 5:793 (abstract no. D.611); Nemoto T, Brown LS, Primm BJ, Foster K, Chu A, Ajuluchukwu D; Addiction Research and Treatment Corporation, New York City, USA; To investigate behavioral risk factors among IVDUs, especially focusing on their drug and sexual behaviors, and psychological status. In 1986, a total 262 subjects; 162 males and 100 females; 107 Blacks, 116 Hispanics, and 35 Whites, were recruited from methadone maintenance clinics in Manhattan and
"Prevalence of prostitution" among female HIV+Ve injecting drug users in Glasgow and the establishment of a health care drop-in centre.: Int Conf AIDS 1989 Jun 4-9; 5:793 (abstract no. D.612); Goldberg DJ, Thomson A, Green ST, Emslie JA, Kennedy DH; CD(S)U & Infectious Diseases Unit, Ruchill Hospital, Glasgow, UK; INTRODUCTION: Epidemiological studies in the USA and Europe repeatedly confirm that the major risk factor determining if an individual prostitute becomes infected with HIV is not sexual activity but if they are injectors. The prevalence of prostitution among 37 known HIV-positive female drug injectors (DIs) (those clea
HIV infection in a detoxification unit.: Int Conf AIDS 1989 Jun 4-9; 5:793 (abstract no. D.613); Muga R, Tor J, Melus R, Ginesta C, Rey-Joly C, Foz M; Service of Internal Medicine. Hospital "Germans Trias i Pujol". Universitat Autonoma de Barcelona. Barcelona, Spain; The greater number of IDAs who start detoxification treatment in Spain come from primary assistance programs, are apparently asymptomatic and don t know the possibility of HIV infection nor its clinical status. We studied, longitudinally, a group of IDAs admitted to a hospital detoxification unit to obtain a
Compliance of medical follow-up in HIV positive patients.: Int Conf AIDS 1989 Jun 4-9; 5:793 (abstract no. D.614); Cummins B, Bisset C, Inglis JM, Brettle RP; HIV Counselling Clinic, Scotland, United Kingdom; To review medical follow-up compliance in HIV positive patients. METHOD: The City Hospital HIV Counselling Clinic offers confidential pre-post test counselling for self referrals. Risk activities were categorised as follows:- injecting drug users (IDU), IDU prescribed/not prescribed Methadone (IDU+METH, IDU
San Francisco Speedballs: an ethnography of cocaine and heroin users at high risk for AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:794 (abstract no. D.615); Biernacki P, Margolis E, Feldman H; Youth Environment Study, Inc., San Francisco, CA, USA; To analyze the practice of speedball (combined cocaine and heroin) use in segments of San Francisco s intravenous drug using (IVDU) population. Interviews with active IVDUs and Community Health Outreach Workers (CHOWs): coding, analyzing and evaluating this data. Speedball use is common amo
Engaging the client with histories of substance abuse or mental illness.: Int Conf AIDS 1989 Jun 4-9; 5:794 (abstract no. D.616); Messina, Chapman, Silva, Gutterman; USA; Unlike many other ambulatory care settings, the AIDS Day Treatment of the Village Nursing Home in New York City has succeeded in engaging the client with histories of substance abuse or mental illness. Success in this engagement process is due to a specific set of program policies, including an assumption that some cli
Support groups for drug users affected by HIV and AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:794 (abstract no. D.617); Cranfield S, Riccio M, Hogarth S, Malloy R, Baracat E; Drugs Education Group, Terrence Higgins Trust, London, England; To establish effective support groups in London for drug users affected by HIV/AIDS and to recommend guidelines for good practice. The Drugs Education Group (DEG) is a voluntary body of professionals working in the field of drug misuse which acts as an advisory group to the Terrence Higgins Trust, E
Swedish and international syringe exchange programmes: evaluation and Swedish recommendations.: Int Conf AIDS 1989 Jun 4-9; 5:794 (abstract no. D.618); Olin R; National Board of Health and Welfare, Stockholm, Sweden; During 1988 the Swedish National Board of Health and Welfare evaluated the ongoing syringe exchange programme (SEP) in the Malmo/Lund region. Available data from BritishSEP (Stimson) and Amsterdam (Bunning) is also considered in this report. The report also gives a picture of the HIV/IVDA situation in Europe. Swedish I
Outreach programs in Norway - appropriate instruments in national HIV/AIDS prevention among drug users.: Int Conf AIDS 1989 Jun 4-9; 5:794 (abstract no. D.619); Nilsen B; Municipal Outreach Program, Oslo, Norway; Recent research reports an accumulation of HIV-infection among drug users using streets and parks in Oslo city ( Norway ) as resorts. These groups often withhold from public care, and are not easily reached by the regular health and welfare system. The main objectives of outreach programs are to seek out and establish
The AIDS Vancouver Island street outreach project.: Int Conf AIDS 1989 Jun 4-9; 5:794 (abstract no. D.620); Williams VG, English J; AIDS Vancouver Island, Victoria, British Columbia, Canada; AIDS Vancouver Island Street Outreach Program investigated the prevalence of high risk behaviours among street youth, sex trade workers, and IVDU s on the streets of Victoria; evaluated their knowledge of and attitudes about HIV/AIDS transmission behaviours and assessed the effectiveness of the street couns
[Presentation of an educative brochure intended for health facility personnel]: Int Conf AIDS 1989 Jun 4-9; 5:795 (abstract no. D.622); Abiteboul D, Matheron S, Pernet M, Schneeberger C; Medecin du travail, France
Surveillance on occupational exposure to HIV among health-care workers in Italy.: Int Conf AIDS 1989 Jun 4-9; 5:795 (abstract no. D.623); Francavilla E, Cadrobbi P, Scaggiante R, De Silvestro G, Bortolotti F, Bertaggia A; Dept. of Infectious Diseases, Padua, Italy; To evaluate the frequency of seroconversion in health-care workers exposed to HIV. During a 22-months period, from March 1987, 641 health care workers who had been exposed to potentially infectious body fluids were screened for anti-HIV by an ELISA test. Risk factors other than occupational were exc
The effect of a dedicated AIDS unit on housestaff attitudes towards AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:795 (abstract no. D.624); Bialer P, Wallack J, Perlman D, Flegenheimer W; Beth Israel Medical Center, New York, NY, USA; A number of previous studies have revealed housestaff anxieties and career changes due to the AIDS epidemic. This study was undertaken to demonstrate the impact of a rotation on a dedicated AIDS unit upon these attitudes. Interns from the Department of Medicine in a 1000 bed urban teaching hospital
Knowledge, attitudes and beliefs of physicians regarding HIV infection in a London health district.: Int Conf AIDS 1989 Jun 4-9; 5:795 (abstract no. D.625); Golding SM, McEvoy M; Department of Health Education, Islington Health Authority, London, England; To assess knowledge about, and attitudes towards HIV, amongst hospital and primary care physicians in the Health District of Islington. A self-administered postal questionnaire was distributed to all physicians in active medical practice within the Health District of Islington. Twenty questions were
AIDS-related knowledge, attitudes and behavior of nurses and hospital administrators: a comparative analysis.: Int Conf AIDS 1989 Jun 4-9; 5:795 (abstract no. D.626); Campbell S, Willenbring K, Henry K; Department of Medicine, St. Paul-Ramsey Medical Center, St. Paul, Minnesota USA; To describe differences and similarities of AIDS-related knowledge, attitudes, and behaviors between registered nurses (RNs) and hospital administrators (HAs) located at one hospital. In September 1987 an anonymous survey was delivered to all RNs and HAs at a 455-bed acute care teaching hospital. In
Implementation and evaluation of universal precautions in a large community hospital.: Int Conf AIDS 1989 Jun 4-9; 5:796 (abstract no. D.627); Lampson J, Klimek J, Malo-Schlegel S, Traficanti K, York E, Cooper B; Hartford Hosp., Hartford, CT, USA; To develop and test a model educational training program for the prevention of blood borne diseases in health care settings. It has been recommended that blood and body fluid precautions be consistently and universally used by health care workers (HCW) for all patients. The concept of universal prec
Estimated risk of HIV infection among surgeons practicing in the New York City area.: Int Conf AIDS 1989 Jun 4-9; 5:794 (abstract no. D.628); Wormser GP, Jain R, Lowenfels AB; New York Medical College, Valhalla, New York, USA; To estimate the occupational risk of HIV infection in surgeons. Surgeons from several hospitals in the New York City area were surveyed by telephone or by mail between July and October 1988 regarding puncture wound injuries occurring within the past 1 year. 100 (91%) of 110 surgeons contact
HIV/AIDS professional education: program development and evaluation for physicians, nurses and social workers.: Int Conf AIDS 1989 Jun 4-9; 5:794 (abstract no. D.629); Hurley PM, Malgady R, McGriff E, Grossman A; New York University, New York, New York, USA; To develop and evaluate a comprehensive program of AIDS education for primary care physicians, nurses and social workers. A basic core curriculum (e.g., immunology, epidemiology/virology, opportunistic infections) was developed and has been revised by clinicians and faculty experienced in HIV/AIDS c
Using learning domains to train health care workers (HCW).: Int Conf AIDS 1989 Jun 4-9; 5:791 (abstract no. D.630); Berman C; University of Illinois at Chicago, Chicago, Illinois, USA; To help trainers improve health care workers learning in three domains; cognitive, attitude and skills. Analysis from observations of over 50 training programs conducted between 1985 and 1988 for multi-discipline health care workers. Programs (experiential exercises and small group discussions) were
Development and results of a needs assessment survey for health care professionals at a municipal hospital.: Int Conf AIDS 1989 Jun 4-9; 5:796 (abstract no. D.631); Akpan S, Noel-Connor J, El-Sadr W; Harlem Hospital Center, New York, N.Y. USA; To determine attitudinal and/or cultural factors that influence the care administered to HIV-infected patients. This was of particular interest since the HIV-infected population at this institution primarily consists of intravenous drug users (IVDUs) and of minorities. Informally, we found out that
Sources of stress among AIDS caregivers.: Int Conf AIDS 1989 Jun 4-9; 5:791 (abstract no. D.632); Mandel JS, Jacobs R, Grade M, Lewis A; University of California at San Francisco, School of Medicine and Nursing, California, USA; To determine common sources of stress among AIDS caregivers. Participants in care for the caregiver workshops were surveyed by self-administered questionnaire to identify sources of perceived stress in the provision of AIDS care. Although a wide range of health care providers were targeted
The effects of an educational program on changing dental care workers attitudes and knowledge regarding HIV.: Int Conf AIDS 1989 Jun 4-9; 5:797 (abstract no. D.633); Andriolo M, Gerbert B, Sumser J, Maguire B; St. Clare's Hospital and Health Center, New York, NY, USA; To assess the change in dental care workers attitudes and knowledge about HIV after attending an educational seminar. Dental care workers were invited to attend a 3 hour seminar which provided information on transmission, etiology and epidemiology of HIV, oral manifestations of HIV and infection con
AIDS education and the health worker: a planning workshop.: Int Conf AIDS 1989 Jun 4-9; 5:797 (abstract no. D.634); Linsk NL, Wright EJ, Youngman G, Miller R, Hefron P, Chen L; Midwest AIDS Education Center, Univ. of Illinois, Chicago USA; (a) Share information about education of health workers in countries of high prevalence of AIDS/HIV; (b) identify critical issues--e.g. curriculum, delivery systems, social & occupational context; national information campaigns; (c) establish global linkages between AIDS health professional educators; (
Team-based model: AIDS Train the Trainer Program for health care professionals.: Int Conf AIDS 1989 Jun 4-9; 5:797 (abstract no. D.635); Bisaillon DA, Fitzsimmons MJ; Massachusetts Hospital Association, Burlington, MA, USA; To describe and evaluate a model AIDS Train the Trainer Program for teams of health care providers in hospitals. METHOD: The Massachusetts Hospital Association in conjunction with the Massachusetts Department of Public Health and The New England AIDS Education and Training Center designed and implemented a t
Attitudes and behaviours of hospital personnel: evaluation of the AIDS prevention campaigns in Switzerland.: Int Conf AIDS 1989 Jun 4-9; 5:797 (abstract no. D.636); Dubois-Arber F, Hausser D, Lehmann P, Gutzwiller F; Inst. univ. de med. sociale et preventive, Lausanne, Suisse; Medical staff in hospitals plays an important role in patient care and in assuming opinion leadership within the population. AIDS related attitudes and behaviours of medical personnel may positively or negatively affect the course of AIDS prevention. Estimation of professional and private risk of contamination is an im
Emotional aspects of the relationship between medical students and AIDS patients at a university hospital.: Int Conf AIDS 1989 Jun 4-9; 5:797 (abstract no. D.638); Talberg J, Morais de Sa CA, Ferreira LE, Magalhaes MC, Miguez LA, Guimaraes-Dias M; Gaffree & Guinle University Hospital - University of Rio de Janeiro (UNI-RIO) - Brazil; To improve the relationship between medical students and AIDS patients in University Hospital ward. Study conducted in a general medicine ward, where up to 50% of the 22 patients load is occupied by AIDS patients. Third-year medical students initiating hospital practice participated, during their fi
Care for the caregivers - a training model.: Int Conf AIDS 1989 Jun 4-9; 5:798 (abstract no. D.639); Jacobs R, Lewis A, Mandel J; Young Adult Institute, New York, N.Y. USA; To develop an educational vehicle which addresses the stress experienced by those who provide AIDS care over time. Simple techniques in recreational and drama therapy have been integrated into a workshop format. These techniques are used to facilitate discussions among participants focusing on feeli
Improving the skills of health care providers who teach.: Int Conf AIDS 1989 Jun 4-9; 5:798 (abstract no. D.640); Downer A, Chapman L, Kaetz S, Mandel L; University of Washington, WAMI AIDS Education and Training Center Program, Seattle, WA, USA; The University of Washington AIDS Education and Training Center Program (AIDS ETC) was established in 1987 to prepare health care workers to teach others about HIV/AIDS. In addition to content on HIV/AIDS, the program offers instruction using the AIDS Teaching Skills Curriculum. This curriculum includes chapters on Cre
Utilizing medical students in a university-based comprehensive AIDS education and counseling program.: Int Conf AIDS 1989 Jun 4-9; 5:798 (abstract no. D.641); Copello AG, Schaffner W, Sheets R, Lindsay L, Nixon L, Sy F; Vanderbilt University, Nashville, Tennessee, USA; To successfully involve medical students in the delivery of AIDS/HIV educational and counseling services as part of the academic program. First, second, and fourth year medical students at Vanderbilt Medical School were enrolled in the elective course, AIDS Education and Prevention. This course is c
Reluctance of health care workers to treat AIDS patients - an analysis.: Int Conf AIDS 1989 Jun 4-9; 5:798 (abstract no. D.642); Currey C, Ogden B, Johnson MP; University of Florida, Gainesville, Florida, USA; To evaluate association between willingness to work with AIDS patients and knowledge of how AIDS is transmitted, ethical beliefs, and fear of contagion. Ninety health professionals were surveyed about their willingness to work with AIDS, their attitudes towards AIDS related ethical issues, and under
AIDS education and training for health care providers in Virginia.: Int Conf AIDS 1989 Jun 4-9; 5:798 (abstract no. D.643); Kaplowitz L, Bradford J, Lesniak L; Medical College of Virginia-Virginia Commonwealth University, Richmond, Virginia, United States; To plan, implement, and evaluate a program of AIDS education and clinical training tailored to the needs of health care providers throughout Virginia. Through cooperation with the Virginia Department of Health and the Mid-Atlantic AIDS Regional Education and Training Center, a multidisciplinary team
[Analysis of biological and medical behavior in the presence of the seropositive drug abuse relation (survey in the Ile de France region]: Int Conf AIDS 1989 Jun 4-9; 5:798 (abstract no. D.644); Naudin C, Neumann C, Malamud P, Labayle D, Rabine C, Debiez C; Association HIV 91.Hopital de Bligny.91640.France
Multidisciplinary AIDS team role in morale and stress reduction among health care workers (HCWs).: Int Conf AIDS 1989 Jun 4-9; 5:799 (abstract no. D.645); Bulkin W, O'Dowd MA, Callan M, Friedland GH, Gordon L, Klein RS; Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA; To describe a multidisciplinary team s role in maintaining morale and reducing stress among AIDS HCWs. DESCRIPTION: Complexity of AIDS clinical care requires expertise from many disciplines and is associated with HCW stress. Stressors include: cruelty of AIDS, youth of patients(pts), inadequate social and fa
AIDS & health care givers: knowledge, attitudes and infection control practices.: Int Conf AIDS 1989 Jun 4-9; 5:799 (abstract no. D.647); Sy F, Burris J, Garrison C, Addy C; University of South Carolina School of Public Health, Columbia, South Carolina, USA; To determine the knowledge, attitudes and infection control practices among health care givers. 241 health care givers, consisting of registered nurses, licensed practical nurses, nursing assistants and technicians, medical technologists and phlebotomists, responded to an AIDS survey in a South Caro
HIV exposed health care workers at MSKCC.: Int Conf AIDS 1989 Jun 4-9; 5:799 (abstract no. D.648); Carrow MJ, Campbell SW, Blevins A, Sobeck KA, Gold JW, Armstrong D; Memorial Sloan-Kettering Cancer Center, New York, New York, USA; To examine compliance of Health Care Workers (HCW) to CDC guidelines for significant exposure (SE) to HIV infected patients. All HCW reporting significant exposures (needlestick or mucous membrane) to employee health service between June 1986 and December 1988 were referred to an Infectious Disease
Support for staff addressing HIV/AIDS issues in a drug rehabilitation centre.: Int Conf AIDS 1989 Jun 4-9; 5:799 (abstract no. D.649); Burgess M; King's College Hospital, London, UK; To enable staff at a drug rehabilitation centre to identify sources of stress arising from their treatment of clients with HIV/AIDS and to generate management solutions. A facilitated support group was set up for all staff, many of whom are themselves ex-users. Fortnightly meetings were held over a
Needs assessment of staff AIDS education program in tertiary care and inner city community hospital.: Int Conf AIDS 1989 Jun 4-9; 5:799 (abstract no. D.650); Lefkowitz-Korenblit P, Foley M, Walther V, Rothenberg A, Martas M, Epstein I; Mount Sinai School of Medicine, New York, NY, USA; To develop an AIDS staff education program in 2 hospitals serving East Harlem, an inner city community: Mount Sinai Hospital, a tertiary care center and North General Hospital, a minority-run community hospital. A comprehensive needs assessment was performed at both sites by 2 AIDS Education Coordin
A support group can help primary caregivers keep pace with the increasing impact of the HIV epidemic.: Int Conf AIDS 1989 Jun 4-9; 5:800 (abstract no. D.651); Makadon HJ, Frost JC, Judd D, Lee S, O'Neill SF, Paulsen RH; Healthcare Associates and the Departments of Medicine, Social Work and Psychiatry, Beth Israel Hospital/Harvard Medical School, Boston, MA, USA; To describe a multidisciplinary support group of health care providers working with people with AIDS in a primary care group practice within a general medical hospital. METHOD: A 15 member group of clinical and administrative personnel met with a mental health group facilitator for 15 weeks. The members disc
[Denial of reality and education of health occupations]: Int Conf AIDS 1989 Jun 4-9; 5:800 (abstract no. D.652); Mettetal JF, Edelmann F, Bary M, Jourdan A; Hopital Necker, Arcat-SIDA, Civis
Effects of HIV and AIDS work on staff involved in caring.: Int Conf AIDS 1989 Jun 4-9; 5:800 (abstract no. D.653); Sherr L; St Mary's Hospital/Roehampton Institute: H George - St Mary's, London, UK; INTRODUCTION: Staff stress and burnout has been well documented. HIV involves health care staff in screening, clinics, counselling and high intensity care. This study examined the impact on staff, how they coped and to see if coping styles and avenues were predictive of adjustment to stress. METHOD: Twenty one staff fr
Cooperation between physician and psychologist: AIDS - a call for psycho-somato-social teamwork.: Int Conf AIDS 1989 Jun 4-9; 5:800 (abstract no. D.654); Leiberich P, Hermann C, Reiser J, Porsch U, Hunzelmann N, Olbrich E; University of Erlangen, Medical Clinic I, Federal Republic of Germany; To describe several ways of cooperation between physicians and psychologists caring for HIV-seropositive persons and AIDS-patients. 100 physicians and psychologists completed an inventory asking (1) for their experiences with HIV-positive persons and (2) their expectations of the other profession. R
Pictural informations are a good way to contribute to health care workers (HCWs) education.: Int Conf AIDS 1989 Jun 4-9; 5:800 (abstract no. D.655); Henrivaux P, Thioux C, Fairon Y, Bruninx G, Hercot R, Roland AM, Renaud M; CHU - Sart Tilman, State University of Liege, Belgium; Working with AIDS can induce stress, anxiety and overwork as well as intellectual stimulation. This psychological impact could be linked to the lifestyles associated with AIDS, distress over the youth of the patients, neurological complications and dying patients. Also, individual assessment of HCWs remained
Psychological strains in a multidisciplinary team taking care of AIDS patients in Brazil.: Int Conf AIDS 1989 Jun 4-9; 5:800 (abstract no. D.656); Souza A, Queiroz A; School of Medicine, Federal University of Rio de Janeiro, Brazil; To identify and work through, by an experience in group dynamics, the anxieties and conflict tensions felt by a health team treating AIDS patients. METHOD: The investigation was done through the structure of the multidisciplinary team dealing with the group dynamics. Weekly meetings with the research subject
Knowledge about HIV transmission in Hispanics in San Francisco: acculturation effects.: Int Conf AIDS 1989 Jun 4-9; 5:801 (abstract no. D.658); Marin B, Marin G; University of California, San Francisco, San Francisco, California, USA; To describe knowledge about AIDS and HIV in a representative sample of Hispanics from San Francisco. A random-digit-dialing telephone survey of 470 Hispanic adults residing in areas with 10% or more Hispanic population was conducted using items from the National Center for Health Statistics. RESULTS
AIDS knowledge levels and related sexual practices among inner-city sexually transmitted disease (STD) clinic patients.: Int Conf AIDS 1989 Jun 4-9; 5:801 (abstract no. D.659); Sambula S, Naillia M, Patrick S, Fordyce EJ, Stoneburner R; New York City Department of Health, New York, USA; To determine the levels of AIDS knowledge and attitudes and the sexual practices of a high risk predominantly black and Hispanic population attending STD clinics in NYC. METHOD: 1178 self-administered questionnaires were completed in STD clinic waiting rooms during a three month period in 1988. Information o
A community approach to HIV prevention in Minnesota's communities of color.: Int Conf AIDS 1989 Jun 4-9; 5:801 (abstract no. D.660); Fletcher P, Heckert K, Moen M, Finn M, Beniak J; The Four Minnesota Minority Councils, Minnesota Department of Health (MDH), Minneapolis, MN, USA; To mobilize intergovernmental and multi-agency collaboration to limit the spread of the human immunodeficiency virus (HIV) among persons of color in Minnesota. In Minnesota, as elsewhere in the U.S., the communities of color are disproportionately affected by HIV. In January 1988, MDH developed a HI
Meeting the health care needs of minorities with HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:801 (abstract no. D.661); Hazard M, Shervington W, Stroud F, Dailey L, Walker S, Scott R; AIDS Minority Health Initiative, USA; To examine the effectiveness of case management services as a primary needs assessment tool for AIDS agencies in planning and implementing non-hospital based AIDS services. METHOD: A demonstration project was established to assess and identify the health care needs, psychosocial needs, and cost of caring for
AIDS education to male sex workers: working at the risk interface.: Int Conf AIDS 1989 Jun 4-9; 5:801 (abstract no. D.662); Hunter A, Overs C; Prostitutes Collective of Victoria, Melbourne, Victoria. Australia; Male sex workers are mostly gay and a significant proportion are IV drug users. Many, particularly the young, don t identify with or become adequately serviced by their communities and relevant organisations. An aim of this project is to involve and educate such groups so that the risk gap presented by male sex work is
Assessing the impact of AIDS intervention projects.: Int Conf AIDS 1989 Jun 4-9; 5:802 (abstract no. D.663); Janowitz B, Williamson NE; Family Health International, Research Triangle Park, NC, USA; Evaluating projects designed to increase knowledge or to change attitudes about AIDS is fairly straightforward. This is not the case if project is designed to prevent HIV transmission. For example, having a control group might make sense scientifically in order to assess what might have occurred without the program. Bu
Factors influencing AIDS risk perception of black gay men.: Int Conf AIDS 1989 Jun 4-9; 5:802 (abstract no. D.664); Mays VM, Cochran SD, Bellinger G; University of California, Los Angeles, California, United States; As AIDS continues to disproportionately affect Blacks in the U.S. (CDC, 1988; Mays & Cochran, 1987), accurate assessment of their perception of risk is needed to design effective risk reduction interventions. One HIV at-risk group for whom we have little empirical knowledge of their lifestyles (Cochran
Marriage, STD and protection from HIV.: Int Conf AIDS 1989 Jun 4-9; 5:802 (abstract no. D.665); Clark CF, Kuhn AC; U of South Florida, Tampa, Fl USA; As the realization has grown that the AIDS Epidemic is unlikely to be controlled anytime soon by either a vaccine or a drug treatment, interest in social behaviors that reduce STD rates has increased. The most acceptable sexual behavior occurs within marriage, and the relative absence of marriage in the young sexually
A public hospital and the AIDS epidemic in Oakland, California.: Int Conf AIDS 1989 Jun 4-9; 5:802 (abstract no. D.666); Seidel EA, Wofsy J, Rose T; Highland Hospital, Dept. Internal Medicine; Oakland, California, USA; To compare diagnoses, ethnicity, and transmission risks of 55 patients presenting to a newly formed outpatient subspecialty HIV clinic at Highland Hospital, Oakland, CA, with similar data already existing for California. Diagnoses, ethnicity, and risk of all 55 patients (51 males and 4 females) seen
Focus groups as an educational intervention for black gay men.: Int Conf AIDS 1989 Jun 4-9; 5:802 (abstract no. D.667); Bellinger G, Mays VM, Cochran SD; Black C.A.R.E. Project, Los Angeles, United States; In order to develop effective strategies for AIDS education and prevention efforts with Black gay and bisexual men, information was gathered from diverse groups across the United States . Six focus groups of 2-5 hours in length, each composed of 8-10 Black gay and bisexual men, were convened in New
Where bottom-up and top-down approaches meet: the development of a comprehensive and integrated health promotion/AIDS prevention program.: Int Conf AIDS 1989 Jun 4-9; 5:802 (abstract no. D.668); Rose D, Lefkowitz P, Belville R, Barrett E, Indyk D, Newman H, White G; Mount Sinai School of Medicine NYC, NY, USA; The paper provides a model for the development of a long-term, dynamic, comprehensive and integrated community based AIDS education/prevention program. A multi-agency CBO was sought which would provide a strategic program site targeted to the multiple needs of young at-risk families and the schools
Stockholm County Council AIDS Prevention Program directed towards ethnical minority groups.: Int Conf AIDS 1989 Jun 4-9; 5:803 (abstract no. D.669); Abascall; Sweden; Sweden is an immigrant country. Out of 8 million inhabitants, around 1 million are immigrants. Some 400,000 of them live in Stockholm County. To reach this group consideration should thus be taken to the ethnical and multicultural aspects such as language, culture, religion, and different views on sexuality. What is
Psychological and sociological correlates of depression in patients with AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:803 (abstract no. D.670); Adinolfi A, Suarez E, Dideriksen P, Bartlett JA; Duke University Medical Center, Durham, N.C., USA; To investigate the relationship between psychological traits and situational factors, and state depression in patients with diagnosed AIDS. Subjects (N=22) were recruited from patients attending our AIDS clinic. All subjects had a diagnosis of AIDS as evidenced by opportunistic infections or wastin
[AIDS stereotyping among university students]: Int Conf AIDS 1989 Jun 4-9; 5:803 (abstract no. D.671); Bayes R, Call J, Losilla JM, Aldavert L, Costa D; Universitat Autonoma de Barcelona, Bellaterra, Barcelona, Espagne
Understanding precautionary and risk-bearing behavior; a model for the development of HIV-prevention programs.: Int Conf AIDS 1989 Jun 4-9; 5:803 (abstract no. D.673); Sandfort T, de Vroome E, Paalman M, Tielman R; Utrecht University, Utrecht, The Netherlands; A sequential phase model has been developed, to understand sexually risk-bearing behavior as well as the adoption of safe sex practices. Behaving precautionary is seen as a result of a conscious decision, reached in several sequential steps, determined by a variety of factors. Putting the decision into actua
[Psychological experiences in HIV positive subjects regarding treatment with zidovudine]: Int Conf AIDS 1989 Jun 4-9; 5:803 (abstract no. D.674); Vicini MA, Meraviglia P, Valsecchi L, Isabella L, Mezzenzana MA, Cargnel A; II Service de Maladies Infectieuses, Hopital L.Sacco, Milan, Italie
[Cognitive and behavioral evaluation of HIV positive subjects]: Int Conf AIDS 1989 Jun 4-9; 5:804 (abstract no. D.675); Zamperetti M, Iuric D, Mastrangelo F, Vergani D; Servizio Psichiatrico di Diagnosi e Cura "Origgi I" e Centro di Medicina Comportamentale, Ospedale C/ga Granda Niguarda, Milano, Italy
Suicidal thoughts and acts in HIV infection suspects.: Int Conf AIDS 1989 Jun 4-9; 5:804 (abstract no. D.676); Parga FJ; Sexually Transmitted Disease Control Programme, San Juan, Puerto Rico; For many persons, infection with Human Immunodeficiency Virus means the immediate development of AIDS and an early death. Suicide becomes a viable alternative to avoid long illness and to obtain control over the situation. In many cases, AIDS will not be developed in years. In order to evaluate the occurrence of suicid
Influence of loneliness and social support on the effectiveness of a behavioral intervention with healthy males at risk for AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:804 (abstract no. D.677); Ingram F, Laperriere A, Ironson G, Antoni M, Fletcher MA, Schneiderman N; University of Miami, Miami, FL, USA; Increased anxiety and depression have been associated with receiving a positive diagnosis for the human immunodeficiency virus (HIV-1). This may serve to further compromise an already vulnerable immune system, resulting in an increased risk for opportunistic infection and worsened disease prognosis. Aerobic
[Evolution of psychological reactions verified in heterosexual asymptomatic seropositives, anti-HIV]: Int Conf AIDS 1989 Jun 4-9; 5:804 (abstract no. D.678); Betscher S, Pesce A, Vinti H, Dujardin P, Bonhomme P, Cassuto JP; Ligue Regionale Francaise de Lutte contre le SIDA - C.H.R.U. Nice, France
Development of a questionnaire to determine knowledge, attitudes and behaviour to HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:804 (abstract no. D.679); Parga FJ; Sexually Transmitted Disease Control Programme, San Juan, Puerto Rico; HIV infection has been called by many a behavioral condition. As long as there is no cure or vaccine, the only alternative to avoid infection with the Human Immunodeficiency Virus is avoiding the practices employed by persons at increased risk for infection. In order to develop educational campaigns, it is necessary to
[Workshop on sexual activities with reduced risk - a new way of life integrated & with sexual health]: Int Conf AIDS 1989 Jun 4-9; 5:804 (abstract no. D.680); Sourdif I, Laroche F; Comite sida aide Montreal, Montreal, Quebec, Canada
Aerobic exercise training attenuates the stress of a positive test for anti-HIV-1.: Int Conf AIDS 1989 Jun 4-9; 5:805 (abstract no. D.681); Laperriere A, Ironson G, Antoni M, Klimas N, Schneiderman N, Fletcher MA; University of Miami, Miami, FL, USA; Receiving a positive test for the anti-human immunodeficiency virus (HIV-1) has been associated with increases in anxiety and depression. This could lead to a further suppression of an already compromised immune system resulting in an increased risk of opportunistic infection and a worsening of disease progn
Measuring and reducing risks of HIV transmission amongst female prostitutes in Birmingham, England.: Int Conf AIDS 1989 Jun 4-9; 5:805 (abstract no. D.682); Kinnell H, Griffiths RK; Community Medicine Department, Central Birmingham Health Authority, United Kingdom; Birmingham is a city of 1 million, with a surrounding conurbation of another 2 million. In 1987 police arrested about 2000 individuals for prostitution offences, 898 in Birmingham. PROJECT: To measure and reduce risks of HIV transmission amongst female prostitutes, an outreach team was employed, assisted by
Seroprevalence among a low HIV prevalence population: HIV screening data from a family planning population.: Int Conf AIDS 1989 Jun 4-9; 5:805 (abstract no. D.683); Newstetter A, Eversley RB, Biernes DL; Planned Parenthood of San Francisco- Alameda, San Francisco, USA; To identify HIV seroprevalence among family planning patients, and to enable family planning providers to plan prevention strategies. HIV screening data from a voluntary screening program of 1310 female family planning patients attending San Francisco Bay Area Planned Parenthood Clinics was analysed
Ethnicity and HIV risk screening data from a family planning population.: Int Conf AIDS 1989 Jun 4-9; 5:805 (abstract no. D.684); Eversley RB, Newstetter A, Biernes DL; The Center For AIDS Prevention Studies, UCSF, San Francisco, USA; To identify ethnic predictors of HIV high risk behaviors in a family planning population, and to enable family planning providers to plan prevention strategies targeted toward specific ethnic groups. Behavioral risk screening data collected from 512 female family planning patients attending San Fran
Rapid condom damage by common sex lubricants.: Int Conf AIDS 1989 Jun 4-9; 5:805 (abstract no. D.685); Voeller B, Coulson AH, Bernstein GS, Nakamura RM; Mariposa Foundation, Topanga, CA, USA; To evaluate the degradation kinetics of sex lubricants upon the strength of latex condoms. Condoms were lubricated along the distal half and tested at intervals for change in their strength. Standard US (ASTM) tensile strength and elongation tests (T&E) were conducted, as were standard internati
HIV transmission to stable female partners of infected males.: Int Conf AIDS 1989 Jun 4-9; 5:805 (abstract no. D.686); Benetucci J, Zarate S, Gomez Carillo M, Corti M, Bases O, Astarloa L, Boxaca M, Weissenbacher M; Muniz Hospital, Buenos Aires, Argentina; To determine the prevalence of HIV infection in female sexual partners of HIV-positive men. A group of 52 women who had consulted the Infectious Diseases Hospital in Buenos Aires City was selected. Their common feature was to be the stable and exclusive sexual partner of a HIV-positive man. These wo
The medical and emotional/support needs of women with HIV.: Int Conf AIDS 1989 Jun 4-9; 5:806 (abstract no. D.687); Dobko T, Benedik B; AIDS Committee of Toronto, Toronto, Ontario, Canada; To describe the medical needs and psychosocial concerns of women with HIV. To describe a model for support groups for women. Initial findings were obtained from 8 members of a support group for women. A further 12 women were given a structured interview. Qualitative analysis revealed the f
Clinical aspects in the first 81 AIDS cases among women in Milan.: Int Conf AIDS 1989 Jun 4-9; 5:806 (abstract no. D.688); Arminio Monforte A, Galli M, Vigevani GM, Vigano P, Saracco A, Rizzardini G, Valsecchi L; Infectious Diseases Clinic, L. Sacco, Milan, Italy; The main risk-factor for AIDS in Italy is IV drug use (IVDU). A considerable number of cases occur in women. Objective of this study is to describe clinical aspects of AIDS in women. 478 cases of AIDS (16.4% of total Italian cases) were recorded from 1984 to 1988 in L. Sacco Hospital, Milan. All inf
On being HIV - positive, European, female, and "non-existent".: Int Conf AIDS 1989 Jun 4-9; 5:806 (abstract no. D.689); Heggs A; Positive Women's Support Group, Copenhagen. Denmark; The main topic of this paper will be the situation of HIV-positive women, (non IV drug abusers) in Denmark , and the trials and tribulations of this group in establishing self- help groups. The underlying theme of the presentation will be the struggle of this group for recognition in a community which, until quite rece
Nice girls do get AIDS: a support system for middle class women with HIV infection.: Int Conf AIDS 1989 Jun 4-9; 5:806 (abstract no. D.690); Nichols S, Prager M; Beth Israel Medical Center, New York City, USA; To present a rationale for and a model of support needs for middle class women in the US: identifying needs, establishing services, and making them available to patients. In early 1988, two seropositive professional women requested that a supportive network be created for middle class women. Existing support
Women and AIDS in Latin American: lessons for their future.: Int Conf AIDS 1989 Jun 4-9; 5:806 (abstract no. D.691); Carovano K, Stone F; Johns Hopkins University/AIDSCOM, Baltimore, MD, USA; To illustrate the importance of developing AIDS prevention interventions targeting non-prostitute women in Latin America. An examination of existing AIDS prevention programs in Latin America reveals a complete absence of programs targeting women not engaged in prostitution. The epidemiology of AIDS
Prevalence of anal intercourse in women at a counseling-testing site.: Int Conf AIDS 1989 Jun 4-9; 5:806 (abstract no. D.692); Connolly D, Brennan A, Winters D, Gocke D; Robert Wood Johnson Medical School, New Brunswick, New Jersey, United States of America; The high risk of HIV-1 transmission by anal intercourse is well-recognized for homosexual men, but the frequency of this activity in heterosexuals is not well known. We questioned 100 consecutive female clients of our HIV counseling and testing service about their participation in anal intercourse a
Evolution ambulatory Sorocaba ambulatory AIDS experience with prostitutes.: Int Conf AIDS 1989 Jun 4-9; 5:807 (abstract no. D.693); Goncalves VL, Dos Anjos RM, Ramos TF, Gomes MC; Brazil; Our study has been realized with 54 promiscuous sexual life women, in a period going from January 1986 to November 1988, and among the following was observed:- 1. Medium age 26,68 years (14 to 41 years). 2. 24 women have children, and among them 67% have more than 01 and less than 05 children. 3. 28 women (51,85%) ment
[The women of Kinshasa and AIDS - a topography of risk]: Int Conf AIDS 1989 Jun 4-9; 5:807 (abstract no. D.694); Rukarangira WN, Walu E, Schoepf BG, Payanzo N; Harvard School of Public Health, Boston, USA
Asian women and AIDS - research issues.: Int Conf AIDS 1989 Jun 4-9; 5:807 (abstract no. D.695); Yoon SY; PhD in Anthropology, New Delhi, India; This paper identifies behavioural research issues on Asian women and AIDS. It points out that current Information/Education for Health strategies used to educate female prostitutes and women about AIDS are often oblivious to the main problem of women s powerlessness. Research is needed on sex tourism and how it contrib
AIDS related knowledge and attitudes in female clients of selected Health Department Clinics, Houston, Texas.: Int Conf AIDS 1989 Jun 4-9; 5:807 (abstract no. D.696); Tortolero S, Crane M, Fornasini M, Smith D, Hardikar S; University of Texas Health Science Ctr, Houston, Texas, USA; To determine AIDS-related knowledge and attitudes in women attending selected City Health Department Clinics in Houston, Texas. Between 2/29 and 9/30/88, 959 women attending Family Planning, Maternity and Sexually Transmitted Disease (STD) clinics completed an anonymous, self-administered risk asses
The sex industry and the AIDS debate - Australian Conference.: Int Conf AIDS 1989 Jun 4-9; 5:807 (abstract no. D.697); Overs C, Bates J; Prostitutes Collective of Victoria, Australia; The Conference aimed at AIDS, to disseminate information to the community and the sex industry and to consolidate and develop the policy required to maintain low infection rates. Conference participants included a range of sex workers and managements, service providers, administrators and policy makers. Keynote address
Condom instructional booklet for female sex workers (an integrated process).: Int Conf AIDS 1989 Jun 4-9; 5:807 (abstract no. D.698); Pareja R, Rosario S; PROCETS, Ministry of Public Health, Santo Domingo, Dominican Republic; For the development of a Condom Instructional Booklet nothing was assumed. A study was first made to determine the knowledge, attitudes and habilities that female sex workers of Santo Domingo, D.R. had in regards to condom use and correct placement steps. Once the errors, negative attitudes, and patterns of use with th
Affective disorders and HIV infection in intravenous drug abusers (IDAs).: Int Conf AIDS 1989 Jun 4-9; 5:808 (abstract no. D.699); Jacas C, Tor J, Muga R, Listar R, Pascual J, Planas P; Service of Internal Medicine. Hospital "Germans Trias i Pujol". Universitat Autonoma de Barcelona. Barcelona, Spain; To evaluate if affective disorders (anxiety and depression) are associated with HIV seropositivity in IDAs that presumably acquired HIV infection by sharing needles practices. We tested anxiety (STAI-Trait) and depression (Beck s Depression Inventory) in 201 inpatient IDAs (148 males and 45 females)
Condom usage decisions among gay men.: Int Conf AIDS 1989 Jun 4-9; 5:808 (abstract no. D.700); Levine M, Brooks C, Siegel K; Bloomfield College, New Jersey, USA; To understand gay men s decisions to use or not use condoms. METHOD: Focused interviews. Analysis of qualitative data from an ongoing study of sexual decision-making among gay men expands our understanding of condom usage within this population. The initial 80 interviews indicate that varying apprai
Condom use among homosexual men.: Int Conf AIDS 1989 Jun 4-9; 5:808 (abstract no. D.701); Golombok S, Rust J; City University, London, UK; To investigate condom use during sexual activity and to identify any problems associated with the use of condoms. A postal questionnaire survey of 262 homosexual men. It was found that, in spite of the general move by homosexual men towards safer sex practices, half of the men continued to
[Sexuality of persons becoming anti-HIV seropositive]: Int Conf AIDS 1989 Jun 4-9; 5:808 (abstract no. D.702); Rochet-Rossello S, Dutey P, Retornaz G, Trepo C; Hopital Hotel-Dieu, France
Counselling intravenous drug abusers about AIDS and HIV-test in the outpatient methadone clinic.: Int Conf AIDS 1989 Jun 4-9; 5:808 (abstract no. D.703); Haas H, Kurz T; Department of Social Psychiatry University Hospital, Zurich, Switzerland; Should the test be recommended to methadone patients and how to deal with ill patients. Therapeutic consultants of 2 centres for drug-addicts were questioned about 97 well-cared for patients. Desperate reactions about a positive test were rare. In 2 of the 3 observed cases the positive test
Accessible health care for IV drug users.: Int Conf AIDS 1989 Jun 4-9; 5:808 (abstract no. D.704); Elovich R, McCarthy M; AIDS Coalition to Unleash Power, New York, New York, USA; To discuss new case management strategies for HIV-infected IV drug users (IVDUs). Interviews with recovering and active addicts, drug treatment professionals, physicians and community based health care workers, ADAPT. DISCUSSION: In evaluating treatment for HIV-infected IVDUs, we need to question ex
Evaluation of a support project for IV drug users with AIDS in Amsterdam.: Int Conf AIDS 1989 Jun 4-9; 5:809 (abstract no. D.705); Van den Boom F, Derks J, Driessen A; Netherlands Institute of Mental Health, Utrecht, Netherlands; Evaluate effectiveness, feasibility and endurance of a support project for IV drug users with AIDS. METHOD: Often IVDU do not have stable relationships or supportive networks. Contact with family of origin may often be disturbed. Referral from the hospital to the home situation can be difficult, because of t
Social characteristics of drug-addict and seropositive female patients for human immunodeficiency virus.: Int Conf AIDS 1989 Jun 4-9; 5:809 (abstract no. D.706); Macias JR, Gomez A, Franchi M, Matar P, Federman L, Astarloa L; Francisco J. Muniz Hospital. Buenos Aires. Argentina; Analysis of the different social characteristics in female patients seropositive for human immunodeficiency virus (VIH) and drug-addiction antecedents. Analytic and critical investigation. Social relations studies of 55 female patients seropositive for human immunodeficiency virus (VIH), who at thei
HIV transmission in simulated conditions of sharing of hypodermic equipment.: Int Conf AIDS 1989 Jun 4-9; 5:809 (abstract no. D.707); Newmeyer J, Drew L, Minor R; Haight-Ashbury Free Clinic, San Francisco, USA; To examine transmission of HIV under simulated conditions of sharing of hypodermic equipment by intravenous drug users. In a laboratory setting, hypodermic equipment was exposed to HIV (free virus) in medium. Attempts were made to culture virus in medium after eight conditions of exposure: (1,2) con
[Contribution of phenomenology and psychoanalysis to the institutional and individual management of drug abusers infected by HIV]: Int Conf AIDS 1989 Jun 4-9; 5:809 (abstract no. D.708); Charles-Nicolas A, Cordonnier JP; Psychiatre, M/aedecin-Directeur du Centre Pierre Nicole, Croix Rouge Francaise, Paris
HIV infection in I.V. drug abusers.: Int Conf AIDS 1989 Jun 4-9; 5:809 (abstract no. D.709); Leite EA, Hamerschlak N, Szterling L, Carvalho MB, Lima MP, Bellucci SB; Centro Corsini. (CCII). Campinas. Sao Paulo. Brazil; To measure the prevalence of the HIV infection in I.V. drug abuse. (spontaneous request). METHOD: The I.V. drug abusers in attendance of CCII are tested for HIV (E + WB). The patients are oriented by a multidisciplinary education and assistance program and the seronegatives are retested more or less in each
AIDS and psychosocial treatment.: Int Conf AIDS 1989 Jun 4-9; 5:809 (abstract no. D.710); Orgnero I; MSW, Private Practice, Calgary Alberta, Canada; An exploratory study systematically examined the hypothesis that a specific social work intervention, Heimler s Social Functioning, would provide effective psychosocial care to persons with AIDS. The method employed to test the hypothesis was a single system design, specifically, the ABA model. The
Use of propranolol for agitated behavior in AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:810 (abstract no. D.711); Shenkman C; St. Vincent's Hospital and Medical Center, NYC, New York, USA; Although AZT is useful for the treatment of HIV encephalopathy, behavioral disturbances in these patients may remain during treatment with this drug. Propranolol has been described to be useful for agitation and other behavioral disturbances associated with Huntington s chorea, a disorder which also affects
Coping with the psychological effects of an HIV infection in patients in obstetrics and gynecology.: Int Conf AIDS 1989 Jun 4-9; 5:810 (abstract no. D.712); Weingart-Jesse B, Schaefer A; Berlin Universitaetsfrauenklinik Rudolf-Virchow, Standort Charlottenburg FU-Berlin, Bundesrepublik Deutschland; AIM: The influence of various psychological and sociological factors such as drug addiction of female patients with an HIV infection and/or their partners, sexual contacts, personality characteristics on their mastering the illness. GROUP EXAMINED: Out of 111 patients with an HIV infection who had special counselling f
Anger: an important psychological measure in HIV disease?: Int Conf AIDS 1989 Jun 4-9; 5:810 (abstract no. D.713); Levy E, Knapp P, Linde R, Mayer K, Stoddard A, McCusker J; Boston University School of Medicine, Boston, MA, USA; To establish baseline values and associations for psychosocial and immune parameters in a cohort of sexually active gay and bisexual men. The first 130 members of a cohort from the FCHC were given a battery of psychosocial questionnaires (including Anger Expression Scale, Hardiness Test, and Profile
Psychological implications among patients when receiving anti HIV positive.: Int Conf AIDS 1989 Jun 4-9; 5:810 (abstract no. D.714); Marques MM, Fontana MA, Gouvea SG, Rossi G, Lima MP, Bellucci SB; Centro Corsini (CCII). Campinas, Sao Paulo, Brazil; In the holistic vision of the work with patients HIV positive, the Psychology division has been developing a global work that has its aim in the patients emotional stability and better quality of life. This approach, plus a therapy that helps the patient to receive the positive results from HIV test, allowed us the con
Psychosocial assessment of minority women at risk for AIDS: a psychometric pilot study.: Int Conf AIDS 1989 Jun 4-9; 5:810 (abstract no. D.715); Nyamathi A, Flaskerud J; University of California, Los Angeles, USA; A pilot study to describe the psychometric evaluation of several instruments designed to assess the major stresses experienced, the coping responses used, the factors affecting these responses and psychosocial outcome with 73 minority drug abusing and homeless women. Reliability, content, convergent
The AIDS dementia complex (ADC) and impairments of ambulatory activity.: Int Conf AIDS 1989 Jun 4-9; 5:810 (abstract no. D.716); Keilp JG, Tryon WW, Brew BJ, Sadler AE, Aronow HA, Price RW, Sidtis JJ; Memorial Sloan-Kettering Cancer Center, NY, NY, USA; To assess the relationship between symptoms of the AIDS dementia complex (ADC) and decrements in overall ambulatory activity. 16 HIV-1 infected patients with early symptoms of the ADC were evaluated with the Neuro-AIDS Study Group neurological history, examination and neuropsychological (NP) battery
[Impact of nurses' attitudes on the psychological state of AIDS patients]: Int Conf AIDS 1989 Jun 4-9; 5:811 (abstract no. D.717); Cournoyer C, de Montigny J, Charpentier S; Infirmiere, Hopital Hotel-Dieu, Montreal, Quebec, Canada
[Psychological impact of AIDS and intervention strategies for AIDS patients]: Int Conf AIDS 1989 Jun 4-9; 5:811 (abstract no. D.718); de Montigny J; Psychologue en pratique privee, Montreal, Quebec, Canada
[Psychological support exercises]: Int Conf AIDS 1989 Jun 4-9; 5:811 (abstract no. D.719); Vertadier A; Association AIDES, Comite de Paris, France
Sexual development and the sero-positive gay male.: Int Conf AIDS 1989 Jun 4-9; 5:811 (abstract no. D.720); Marcotte G, Valentich M; AIDS Calgary, Calgary, Alberta, Canada; To describe the impact of sero-conversion upon the sexual development of the gay male. Implications for the involvement of social work are discussed. METHOD: A literature review and intensive, unstructured interviews with 3 Western Canadian sero-positive gay males. Additionally, the senior author draws on ba
[Sexual behavior - has it changed because of AIDS? Study of the Zairean population]: Int Conf AIDS 1989 Jun 4-9; 5:811 (abstract no. D.721); Lusakulira N, Moore M, Bertrand WE, Hassig SE, Kashala TD; Ecole de Sante Publique, Universite de Kinshasa/Zaire
Frequency of sexual intercourse according to marital status in the general population.: Int Conf AIDS 1989 Jun 4-9; 5:811 (abstract no. D.722); Magnus P, Stigum H, Sundet JM, Kvalem IL, Gronnesby JK, Bakketeig LS; Department of Epidemiology, National Institute of Public Health, Oslo, Norway; To understand the potential for HIV spread in the general population, empirical data on patterns of sexual behavior are needed. We here present data on the relative frequency of sexual intercourse in heterosexual relationships with respect to present marital status. METHOD: The results are based on the respo
Sexual practices and HIV infection in some Zambian prisons.: Int Conf AIDS 1989 Jun 4-9; 5:812 (abstract no. D.723); Maboshe M, Simooya O, Hira S, Chimfwembe E, Perine P, Mukunyandela M; Tropical Diseases Research Centre, Ndola, Zambia; 1. To establish seroprevalence of HIV infection among inmates in 5 urban prisons in Zambia . 2. To study their sexual practices. All inmates who had been in prisons for at least 3 months were entered in the study and their clinical, social and sexual information was collected. Serum samples were sc
Overview of WHO/UNESCO experience in initiating school health education programmes to prevent AIDS and other STDs.: Int Conf AIDS 1989 Jun 4-9; 5:812 (abstract no. D.724); Pokrovsky A, Bennett D; UNESCO Education for the Prevention of AIDS Programme, UNESCO Paris; The National AIDS Plans of developing countries will be analysed with a view of identifying the extent to which they include formal school AIDS education programmes; the dimensions of the problems these programmes are intended to address; and the essential results, if any, that have been obtained by these programmes up
Alcohol and HIV disease progression in intravenous drug addicts.: Int Conf AIDS 1989 Jun 4-9; 5:812 (abstract no. D.725); Lake-Bakaar G, Rao RS; SUNY-Health Science Center, Brooklyn, N.Y.; Alcohol weakens ego controls and is lymphocytotoxic. It might therefore increase the risk of exposure to HIV as well as accelerate disease progression. To evaluate the effect of alcohol on the rate of HIV infection and on HIV disease progression. Alcohol history evaluated prospectively in 201 IVDA
Cultural differences in AIDS-related attitudes, beliefs, and behavior change.: Int Conf AIDS 1989 Jun 4-9; 5:886 (abstract no. E.501); Zimmerman RS, Stevens RA, Fischl M; University of Miami, Coral Gables, Florida, United States; To assess differences among Hispanics, non-Hispanic Whites (Anglos), American Blacks, and Caribbean Blacks on AIDS-related attitudes and beliefs and their impact on reported behavior change. A subsample (N=140) of respondents recruited in primary care and STD clinics for a high-risk heterosexual tra
Community-based projects for HIV prevention: types of organizations, target populations, and educational strategies.: Int Conf AIDS 1989 Jun 4-9; 5:886 (abstract no. E.502); Kohmescher R, Martich FA, Dietz SE, West GR; Centers for Disease Control, Atlanta, GA, USA; To provide financial support and technical assistance to minority and other community-based HIV prevention projects. On January 9, 1989, the Centers for Disease Control (CDC) announced the availability of funds for minority and other community-based HIV prevention projects. These projects will be co
Fostering public-private partnerships: the New York City Experience.: Int Conf AIDS 1989 Jun 4-9; 5:886 (abstract no. E.503); Omi J, Jacques C, Rautenberg E, Hayes-Cozier R; New York City Department of Health, New York City, USA; To describe the design and outcome of the NYCDOH technical assistance initiative for community based organizations under contract to provide AIDS prevention services. During the 12 month fiscal year 1988-89, NYCDOH let 30 contracts with community based organizations for the provision of AIDS educati
The impact on children whose parents or loved ones have HIV disease.: Int Conf AIDS 1989 Jun 4-9; 5:886 (abstract no. E.504); Sinclair R; Private Practice, counselor and consultant, Cascade AIDS Project, Portland, Oregon, USA; To describe the unique personal, social, and familial issues children face when a parent or adult friend has HIV disease. Interviews and counseling sessions with male and female children between the ages of 10-18 years and their parent, relative, or adult friend. Information gathered indica
Societal impact of HIV infection in women.: Int Conf AIDS 1989 Jun 4-9; 5:886 (abstract no. E.505); Danforth S, Carpenter CC; Brown University Medical Center, Providence, RI USA; To study the impact of HIV infection on the families of affected women. 31 HIV-seropositive women, 26 infected by intravenous drug use (IVDU) and 5 by heterosexual transmission (HT), were thoroughly evaluated and monitored for 22-months (mean) in a multidisciplinary ambulatory care program. Intrafam
Evaluation of care provision to AIDS patients and psychological problems of relatives of (deceased) AIDS patients.: Int Conf AIDS 1989 Jun 4-9; 5:886 (abstract no. E.506); van den Boom FM, Roozenburg H; Netherlands Institute of Mental Health, Utrecht, Netherlands; Evaluate hospital and homebased care; inventory of psychological problems and coping mechanisms of relatives. By striving for more homebased and less hospital care, the pressure on the support system (partners, family, friends) will only increase, meaning that the patient s social network will be increasingl
Psychosocial status of partners of haemophiliacs with HIV infection: a controlled investigation.: Int Conf AIDS 1989 Jun 4-9; 5:887 (abstract no. E.507); Klimes I, Catalan J, Bond A, Garrod A, Day A, Rizza C; Dept. Clinical Psychology, Warneford Hospital, Oxford, UK; To establish prevalence of psychosocial problems in partners of HIV+ haemophiliacs; to compare it with that of the infected partners; to identify the characteristics of those with problems; to assess the need for further psychological intervention. Partners (n=36) of randomly selected and matched fo
A longitudinal study examining the impact of AIDS and HIV on partners and friends - results from baseline, three and nine month retest data.: Int Conf AIDS 1989 Jun 4-9; 5:887 (abstract no. E.509); Church J, Kocsis AE, Vearnals SF, Green J; Paterson Wing, St Mary's Hospital, London, W2 1NY, UK; To assist with targeting support for informal carers by 1) Determining anxiety and depression levels in carers 2) Establishing the degree of role, habit and attitudinal changes experienced 3) Isolating factors predictive of positive or negative outcome. METHOD: Informal carers of PWA s and healthy seroposit
The impact of an AIDS diagnosis on patients and their social support system.: Int Conf AIDS 1989 Jun 4-9; 5:887 (abstract no. E.510); Laquidara K, Jankowski S, Videka-Sherman L; Albany Medical Center, Albany, New York, USA; The primary purpose of this study was to obtain information about the impact of the diagnosis of AIDS on the social support system of persons with AIDS (PWA s). METHOD: Qualitative semi-structured interviews with fifteen PWA s and their primary helpers were completed to obtain thorough and specific descripti
Prevention methodologies for women at risk for AIDS.: Int Conf AIDS 1989 Jun 4-9; 5:887 (abstract no. E.512); Arenson C, Finnegan LP; Thomas Jefferson University, Philadelphia, Pennsylvania, USA; As part of on-going efforts to prevent HIV infection in women at risk, 42 drug dependent pregnant and post-partum women receiving comprehensive treatment were surveyed. Drug use history revealed that 95% are current or past IV drug users with an average of 12 years of needle sharing; 82% of the subjects reported cessat
AIDS and the changing American family.: Int Conf AIDS 1989 Jun 4-9; 5:888 (abstract no. E.513); Levine C; Citizens Commission on AIDS for New York City and Northern New Jersey, New York, NY, USA; A major crisis like AIDS heightens processes of social change already underway and sets in motion new, particularized responses. This presentation will explore the potential impact of some of these changes on American families. I. Who Counts as Family ? Along with other social and cultural factors, AIDS is contributing
NGOs and AIDS - moving from apathy to action: two years of experience from the League of Red Cross and Red Crescent Societies.: Int Conf AIDS 1989 Jun 4-9; 5:888 (abstract no. E.514); Dick B, Wallace B; Community Health Department, League of Red Cross & Red Crescent Societies Geneva, Switzerland; ABSTRACT: NGOs have been, and will continue to be at the forefront of the AIDS pandemic in the fields of prevention, counselling, care and counter-acting discrimination. However, this involvement with AIDS has not always been easy, sometimes because of pre-existing problems, sometimes as a result of relatively new diff
[Risk practices and AIDS in the spanish population]: Int Conf AIDS 1989 Jun 4-9; 5:888 (abstract no. E.515); Usieto R, Sastre J, Torres J, Anabitarte H, de Andres R, Contreras MC; Universite Internationale Menendez Pelayo, Madrid, Espana
[A socio-anthropological approach to AIDS in France]: Int Conf AIDS 1989 Jun 4-9; 5:888 (abstract no. E.516); Marty L; Observatoire Regional de la Sante du Nord-Pas-de-Calais, Lille, France
A contribution to the development of a black anthropological perspective on community-based AIDS education using the experience of combat in Toronto, Ontario, Canada.: Int Conf AIDS 1989 Jun 4-9; 5:888 (abstract no. E.517); Wilson JA, Stevenson B, Phokabye G, Guishard L; Ph.D Candidate Department of Anthropology, University of Toronto, Toronto, Ontario, Canada; An analysis is provided of some of the major problems associated with developing and delivering culturally sensitive AIDS education information in a multi-ethnic Black community situated within a multi-racial society. AIDS in the Black community presents a unique epidemiological configuration since,
An ethnographic study of the sexual practices and drug behaviors of intravenous drug using male prostitutes.: Int Conf AIDS 1989 Jun 4-9; 5:889 (abstract no. E.519); Rucker RD, Nemeth G; Cincinnati Health Department: East Central AIDS Education and Training Center and REACH Program, the National Institute of Drug Abuse (NIDA), Cincinnati, Ohio, USA; Despite a widespread belief that safer sexual practices have been adopted by many gay and bisexual men, little research has been conducted to determine the extent of behavior change that has occurred among male prostitutes since the appearance of AIDS. Additional knowledge in this area is important to determine what fa
Cultural context of HIV infection among homeless families in New York City.: Int Conf AIDS 1989 Jun 4-9; 5:889 (abstract no. E.520); Susser I; Community Health Education, Hunter College and HIV Center for Clinical and Behavioral Studies, New York, New York, USA

5th International AIDS Conference

Montreal, Quebec, Canada — Jun 4-9, 1989