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5th International AIDS ConferenceMontreal, Quebec, Canada — Jun 4-9, 1989 |
Int Conf AIDS 1989 Jun 4-9; 5:166 (abstract no. A.534)
Genesca J, Viladomiu L, Talavera JC, Shih J, Alter H, Esteban JI
Department of Transfusion Medicine, NIH, Bethesda, MD, USA
OBJECTIVE: To determine the prevalence of HTLV-I/II infection in Spanish populations at high-risk for HIV infection.
METHODS: Serum samples were obtained from 150 subjects (1987-88) distributed in 110 drug users, 15 heterosexual contacts, 22 homosexuals and 3 post-transfusional HIV infections; 93% of them were anti-HIV+ and 10% were symptomatic (ARC/AIDS). Anti-HTLV-I were tested by 3 licensed EIAs (Abbott, Dupont, Cellular Prod.). Repeatedly reactive (RR) samples were confirmed with WB, RIPA and a recombinant p21E (env) WB (Biotech). A + result was defined as the presence of p24 and an env product in any of the 3 tests.
RESULTS: Nine samples (drug users) were RR in at least one of the EIAs: Only 4 samples (2.6% drug us.) were interpreted as + (No.1, 2, 4, 7). None of the EIAs was + in all 4 samples. All + samples were detected by the recombinant WB assay.
| PATIENT | |||||||||
| TEST | 1 | 2 | 3 | 4 | 5 | 6 | 7/td> | 8 | 9 |
| EIA 1 | RR | RR | -- | RR | -- | RR | -- | -- | -- |
| EIA 2 | ± | RR | RR | RR | RR | -- | RR | RR | RR |
| EIA 3 | -- | RR | RR | RR | -- | -- | RR | -- | RR |
| WB | p24 | p19,24 | -- | p24 | p19 | -- | p19,24 | p24 | -- |
| RIPA | gp68 | gp68 | -- | gp68 | -- | -- | -- | -- | -- |
| p21E | + | + | -- | + | -- | -- | + | -- | -- |
CONCLUSION: 1) The prevalence of HTLV-I/II infection in HIV infected groups in Spain is very low; 2) Additional confirmatory testing for HTLV-I/II infection is absolutely necessary; 3) The recombinant p21E WB assay might be a useful confirmatory technique for HTLV-I/II infection.
Keywords: AEGIS, Human T-lymphotropic virus 1, Deltaretrovirus Antibodies, Deltaretrovirus Infections, Deltaretrovirus, HIV Infections, Immunoenzyme Techniques, Acquired Immunodeficiency Syndrome, Radioimmunoprecipitation Assay, Prevalence, Spain, ICA5
1989-06-04
A534
Copyright © 1989 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.
