AEGiS-05IAC: HIV seropositive men infected with multiple CMV strains: a longitudinal study of the relationship to T-cell subsets.

5th International AIDS Conference

Montreal, Quebec, Canada — Jun 4-9, 1989

HIV seropositive men infected with multiple CMV strains: a longitudinal study of the relationship to T-cell subsets.

Int Conf AIDS 1989 Jun 4-9; 5:167 (abstract no. A.540)
Leach C, Detels R, Visscher B, Giorgi J, Cherry J; UCLA Sch. of Medicine, Los Angeles, CA, USA

OBJECTIVE: To investigate the relationship between serial infection with multiple CMV strains and T-cell subsets.

METHODS: We identified 56 asymptomatic HIV-1 seropositive homosexual men participating in a study of HIV-1 infection who excreted CMV from semen at two visits 5 to 9 months apart. CMV DNA was isolated from paired isolates in six men, cut with restriction enzyme and then subjected to junctional hybridization. Lymphocyte subsets were determined by flow cytometry.

RESULTS: Paired isolates from each of 2 men gave identical banding patterns (Group 1). Four men were found to excrete a non-identical strain at the second visit (Group 2). Both groups had lower CD4 and CD8 counts at the second visit (mean decrease in CD4: 27% (Group 1), 35% (Group 2); mean decrease in CD8: 14% (Group 1), 33% (Group 2)).

CONCLUSION: Serial excretion of multiple CMV strains occurs in asymptomatic HIV infected homosexuals. All 6 subjects persistently shedding CMV had decreases in CD4 and CD8 cell counts. Evaluation of the remaining CMV isolates (in progress) is necessary in order to determine whether men with multiple strains have different changes in T-cell subsets than men with identical strains.

Keywords: AEGIS, HIV Seropositivity, Cytomegalovirus, T-Lymphocyte Subsets, Longitudinal Studies, Antineoplastic Combined Chemotherapy Protocols, Homosexuality, HIV-1, Antigens, CD8, Cytomegalovirus Infections, HIV Infections, HIV, HIV Antibodies, Antigens, CD4, HIV Seronegativity, MEC protocol 1, Human, Male, immunology, ICA5

890604
A540