5th International AIDS Conference Montreal, Quebec, Canada — Jun 4-9, 1989

Int Conf AIDS 1989 Jun 4-9; 5:177 (abstract no. A.599)
Corless I, Lisker A, Buckheit RW, Smiley ML, Langlois AJ, Weinhold K; University of North Carolina-Chapel Hill, North Carolina

OBJECTIVE: Manikins are used widely for cardiopulmonary resuscitation (CPR) training. Simulating a "worst-case scenario", a manikin was contaminated in different experiments with either cell-free or cell associated HIV-1. Recommended decontamination procedures were compromised by maintaining a wet surface for 10 or 5 sec rather than the recommended 30 sec. The experiment also addressed the relative contributions of chemical disinfection and the mechanical action of wiping.

METHOD: The manikin was contaminated with either a virus saturated swab containing cell-free HIV-1 (5x10(5) infectious units/ml) or with a pipette containing cell-associated HIV-1 (5x10(6) chronically infected CEM cells/ml). The decontamination procedure consisted of 70% isopropyl alcohol sponge or spray or dry 4x4 sponge for 10 or 5 sec, drying time of 30 sec and 5 sec of wiping the surface. After each maneuver a sample from the manikin surface was obtained, cultured in AA5 or CEM cells and the presence of virus determined by reverse transcriptase assay and by quantitative p24 antigen detection (ELISA).

RESULTS: HIV-1 was detected after manikin contamination but not after disinfection with alcohol for 10 or 5 sec. HIV-1 was detected both after initial contamination and after wiping with a dry 4x4 sterile sponge.

CONCLUSIONS: Decontamination of an HIV-1 cell free or cell associated contaminated CPR manikin was achieved in 5 sec with either a 70% isopropyl alcohol sponge or spray, but not using only a dry 4x4 sponge demonstrating the importance of chemical disinfection.

890604
A599

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