5th International AIDS Conference


Montreal, Quebec, Canada — Jun 4-9, 1989

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Radioresistance of HIV-1 in a bone allograft model.

Int Conf AIDS 1989 Jun 4-9; 5:177 (abstract no. A.600)
Conway B, Tomford WW, Hirsch MS, Schooley RT; Infectious Diseases Unit, Massachusetts General Hospital Boston, MA, USA

OBJECTIVE: To study the feasibility of inactivating HIV-1 in bone graft material by gamma irradiation.

METHODS: Serial dilutions of HIV-1 (10(1)-10(6) TCID50/ml) were subjected to gamma irradiation under conditions simulating those within a bone allograft. Radiated and control specimens were cultured on H9 cells which were subsequently evaluated for cytopathic effect and p24 antigen production.

RESULTS: High titers of virus (10(6) TCID50/ml) were not inactivated by gamma irradiation up to 0.55 MRad. p24 antigen values (ng/ml) for lower titered radiated (R) and control (C) virus exposed to 0.4 MRad is shown: TABULAR DATA, SEE ABSTRACT VOLUME. Cytopathic effect was observed in all cultures in which p24 antigen production was detected.

CONCLUSION: Doses of gamma irradiation previously recommended to inactivate many viruses including HIV-1 appear inadequate in this model. Higher doses or alternate methods of ensuring allograft sterility with respect to HIV-1 may be required.

Keywords: AEGIS, HIV-1, Transplantation, Homologous, Bone and Bones, Bone Transplantation, transplantation, surgery, ICA5KWDaegis,hiv-1,transplantation,homologous,boneandbones,bonetransplantation,transplantation,surgery,ica5

890604
A600

Copyright © 1989 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.