6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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Human chromosome 12 supports elevated HIV-1 and HIV-2 trans-activation but not HTLV-I and -II trans-activation in human-hamster hybrid cells.

Int Conf AIDS 1990 Jun 20-23; 6:322 (abstract no. 1034)
Hart C, Galphin J, Westhafer M, Ou CY, Schochetman G; HIV/AIDS Division, Centers for Disease Control, Atlanta, GA 30333, USA

OBJECTIVES: To determine if human chromosome 12 in human-hamster hybrid cells supports elevated HIV-2 tat- and HTLV-I or -II tax-directed trans-activation.

METHODS: Human lymphocyte-Chinese hamster ovary cell hybrids with a defined human chromosome content were cotransfected with a plasmid DNA containing a CAT gene driven by an LTR from HIV-1, HIV-2, HTLV-I, or HTLV-II and a plasmid containing the corresponding tat or tax gene under control of the SV40 early promoter. Levels of trans-activation of the LTRs were measured by an in vitro CAT assay.

RESULTS: We previously showed the presence of human chromosome 12 in human-hamster hybrid cells was required for elevated HIV-1 tat-directed trans-activation of the HIV-1 LTR. In the present study we also found that elevated HIV-2 tat-directed trans-activation of the HIV 2 LTR occurred only in hybrid cells containing human chromosome 12. Elevated HTLV-I and -II tax-directed trans-activation of their respective LTRs, however, did not correspond to the presence of human chromosome 12. In addition, HTLV-I tax trans-activation of the HIV-1 LTR was not supported in hybrid cells containing human chromosome 12.

CONCLUSIONS: Our results indicate that elevated levels of tat-directed trans-activation of HIV-1/-2 LTRs are regulated by a gene(s) encoded on human chromosome 12; regulation of HTLV-I/ -II tax-directed trans-activation requires the presence of a different or additional human chromosome(s).

Keywords: AEGIS, HIV-2, Human T-lymphotropic virus 1, HIV-1, HIV Long Terminal Repeat, Chromosomes, Human, Pair 12, Hybrid Cells, Genes, pX, Chimera, Human T-lymphotropic virus 2, Hamsters, Animal, Cats, Human, In Vitro, Female, genetics, ICA6KWDaegis,hiv-2,humant-lymphotropicvirus1,hiv-1,hivlongterminalrepeat,chromosomes,human,pair12,hybridcells,genes,px,chimera,humant-lymphotropicvirus2,hamsters,animal,cats,human,invitro,female,genetics,ica6

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