6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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Comparison of various drugs effect on HIV reproduction on primary and chronically infected cell lines.

Int Conf AIDS 1990 Jun 20-23; 6:332 (abstract no. 1075)
Pokrovsky A, Plyasunova O, Yastrebova O, Chaplygina S; All-Union Research Institute of Molecular Biology, Koltsovo, USSR

OBJECTIVE: Two stage scheme of anti-HIV drugs screening is proposed. The first stage is performed on primary infected cells (line MT-4 or peripheral blood mononuclear cells). For the second stage assay a cell line chronically infected by HIV-1 and contained provirus is used.

METHODS: The level of HIV reproduction was examined by measuring of reverse transcriptase activity. Virus antigen expression and percentage of infected cells were determined by immunoenzyme and immunofluorescent methods, respectively.

RESULTS: Comparison of drugs with different mechanisms of action on HIV reproduction was performed. Simultaneously the viability of treated cells was determined. On primary HIV-1 infected cells the virus inhibiting effects were for azidothymidine - 90-97%, heparin - 98-99%, glycyrrhizic acid - 95-97%, antisense oligonucleotides - 80-90%, synthetic peptides from binding site of gp120 - 60-70%. On chronically infected cell line the curing effect of these drugs decreased in different extent. Combinations of drugs with various mechanisms of action were also examined.

CONCLUSION: The drugs and their combinations with clear inhibitory effect on both stages of the screening are perspectively for AIDS treatment.

Keywords: AEGIS, HIV, HIV-1, Cell Line, RNA-Directed DNA Polymerase, Anti-HIV Agents, Zidovudine, Proviruses, drug effects, HIV Core Protein p24, Acquired Immunodeficiency Syndrome, HIV Long Terminal Repeat, Reverse Transcriptase Inhibitors, HIV Envelope Protein gp120, HIV Infections, HIV Protease Inhibitors, pathogenicity, pharmacology, genetics, ICA6

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