6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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Parameters of HIV inactivation by disinfectants.

Int Conf AIDS 1990 Jun 20-23; 6:333 (abstract no. 1076)
Busnel RG, Aranda-Anzaldo A, Viza D, Hutchinson SA; Laboratoire d'Immunobiologie, Faculte de Medecine, Paris, France

OBJECTIVE: Comparative study of HIV inactivation by Triton x-100, Benzalkonium chloride, Nonoxynol-9, alcohol, or sodium hypochlorite in relation to time of incubation and viral concentration.

METHODS: Various concentrations of HIV were incubated anywhere from 30" to 10' with various dilutions of the different compounds and were used to infect lymphoblastoid cells.

RESULTS: HIV inactivation was obtained in a relatively short time with most compounds when low concentrations of virus were used. For high concentrations, the time of incubation, the amount and the nature of disinfectant were of the essence. For instance, ethanol destroys viral concentrations of 0.23 ng of p24/ml, but it fails to inactivate concentrations of 6 ng of p24/ml, whilst Triton x-100 at 0.5% and Na hypochlorite at 0.5% inactivate 56 ng of p24/ml, providing that the incubation time exceeds 1'.

CONCLUSION: HIV concentrations in biological fluids being low, inactivation can be easily obtained. However, high viral concentrations, encountered in research laboratories, are more difficult to inactivate in a short time and thus require special care in handling.

Keywords: AEGIS, HIV, Disinfectants, Sodium Hypochlorite, HIV Infections, Benzalkonium Compounds, Antiviral Agents, Reverse Transcriptase Inhibitors, Nonoxynol, Spermatocidal Agents, Octoxynol, virology, ICA6KWDaegis,hiv,disinfectants,sodiumhypochlorite,hivinfections,benzalkoniumcompounds,antiviralagents,reversetranscriptaseinhibitors,nonoxynol,spermatocidalagents,octoxynol,virology,ica6

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Copyright © 1990 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.