6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

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Defining the early events of HIV infection.

Int Conf AIDS 1990 Jun 20-23; 6:334 (abstract no. 1080)
Alter HJ, Allain JP, Eichberg J; NIH, Bethesda, MD, USA

OBJECTIVE: To generate large volumes of plasma/lymphocytes in early phase of HIV infection and to compare sensitivity of standard and new assays.

METHODS: A chimp was inoculated with 38 TCID50 (10 CID50) HTLV-IIIB and apheresed 1-2 times per week. Serial samples were tested for anti-HIV by licensed EIA & Western blot (WB) and for recombinant HIV proteins by dot blot assays; quantitative anti-p24; serum p24 antigen (Ag); anti-nef; neutralizing antibody (NA); viral culture and plasma PCR.

RESULTS: TABULAR DATA, SEE ABSTRACT VOLUME.

CONCLUSIONS: (1) There is a distinct sequence of events with cult=PCR greater than WB greater than anti-p24 capture greater than anti-nef greater than dot blot; all were superior to standard EIA; (2) HIV-Ag did not narrow the window; (3) recombinant dot blot may be good alternative to WB; (4) early plasma now available in volume for assay evaluation.

Keywords: AEGIS, HIV Infections, HIV Antibodies, HIV, HIV Core Protein p24, Blotting, Western, HIV Antigens, HIV Seroprevalence, Deltaretrovirus, Polymerase Chain Reaction, Sensitivity and Specificity, ICA6KWDaegis,hivinfections,hivantibodies,hiv,hivcoreproteinp24,blotting,western,hivantigens,hivseroprevalence,deltaretrovirus,polymerasechainreaction,sensitivityandspecificity,ica6

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