Antibody response to an immunodominant epitope of the HIV-1 specific out protein: correlation of antibody titres to clinical stages.
Int Conf AIDS 1990 Jun 20-23; 6:334 (abstract no. 1083) Schneider T, Hildebrandt P, Ronspeck W, Schauzu M, Weigelt W, Pauli G; AIDS-Zentrum am Bundesgesundheitsamt, Berlin, FRG
A peptide scan based on overlapping decapeptides of the out sequences of two HIV-1 strains (HXB2, ELI) was used to identify an immunodominant epitope. An average of 21% of HIV-1 antibody positive sera obtained from individuals in different stages of the disease reacted with peptides corresponding to the C-terminal amino acid sequence of out. Some of these positive sera reacted with additional peptides derived from internal sequences. The oligopeptide VEMGVEMGHHAPWDVDDL representing the C-terminus of out (HIV-1 strain HXB2) was used for the screening of sera in an ELISA. 243 HIV-1 seropositive and 75 negative sera were investigated. None of the HIV-1 negative sera reacted with the oligopeptide in contrast to 26% of the positive sera. Correlation of reactivity of sera with the Walter Reed staging classification demonstrated that individuals in WR 1 (36%) and WR 2 (42%) were more often reactive than patients classified WR 3-6 (11%). Prevalence of out antibodies in sequential sera of HIV-1 infected individuals will be shown.
Keywords: AEGIS, HIV-1, Immunodominant Epitopes, HIV Seropositivity, HIV Envelope Protein gp41, Antibody Formation, Gene Products, vpu, HIV Envelope Protein gp120, Proteins, Gene Products, vif, Peptides, HIV Envelope Protein gp160, Gene Products, gag, Gene Products, env, Retroviridae Proteins, Enzyme-Linked Immunosorbent Assay, Human, immunology, ICA6
