AEGiS-06IAC: Elispot assay for clonal analyses of HIV-1 specific mucosal and circulating B cell repertoires in primates.

6th International AIDS Conference

San Francisco, California, USA — June 20-23, 1990

Elispot assay for clonal analyses of HIV-1 specific mucosal and circulating B cell repertoires in primates.

Int Conf AIDS 1990 Jun 20-23; 6:335 (abstract no. 1084)
Eriksson K, Nordstrom I, Larsson L, Kilander A, Nordkrans G, Hagberg C, Vahlne A, Jeanson S, Holmgren J, Czerkinsky C; University of Goteborg, Gothenburg, Sweden

OBJECTIVE: To document, in functional terms, the dynamics of B cell activation at mucosal and extramucosal sites in the HIV compromised host.

METHODS: Pilot experiments were performed in immunized macaques to establish the feasibility of a method, the ELISPOT test, for detection of HIV-1 specific B cells. Next, we are following the occurrence and changes in the size(s) and isotype commitment of total as well as HIV-1 specific (gp120, gp41 and synthetic peptides thereof) immunoglobulin-producing B cells in suspensions obtained from mucosal specimens amenable to safe biopsy in humans (duodenal and rectal mucosa). RESULTS AND

CONCLUSION: We have established the feasibility of single cell analyses in humans to document possible changes in the size and composition of HIV-1 specific B cell repertoires in the infected host. Results obtained from comparative ELISPOT analyses of activated B cells in the circulation and gastrointestinal tract of HIV-1-infected individuals will be discussed as to their relevance to the pathogenesis and natural history of AIDS.

Keywords: AEGIS, HIV-1, B-Lymphocytes, Acquired Immunodeficiency Syndrome, Antibody-Producing Cells, Mucous Membrane, Primates, Enzyme-Linked Immunosorbent Assay, Immunoglobulin Isotypes, Animal, Human, analysis, ICA6

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