AEGiS-06IAC: Human immunodeficiency virus replicates selectively in human monoclonal CD4+ lymphocytes subpopulations.

6th International AIDS Conference

San Francisco, California, USA — June 20-23, 1990

Human immunodeficiency virus replicates selectively in human monoclonal CD4+ lymphocytes subpopulations.

Int Conf AIDS 1990 Jun 20-23; 6:338 (abstract no. 1097)
Chapel A, Bensussan A, Bourge JF, Vilmer E, Dormont D; CRSSA CEA DPS/SPE 92265 Fontenay aux Roses, France

OBJECTIVE: In order to investigate viral entrance of HIV1 into susceptible cells, we performed a screening of human monoclonal CD4+ lymphocytes susceptibility to HIV.

METHODS: 12 human CD4+ clones have been characterized and tested regarding their susceptibility to two HIV1 strains. Viral replication was sequentially studied by reverse transcriptase assay, immuno-capture (P24), immunofluorescence, over a 21 days postinfection period. Integration of proviral sequences was evaluated by molecular hybridization (DOT blot) and Polymerase Chain Reaction in Gag, Pol and Env regions.

RESULTS: Three different types of clones were identifiable: 5 clones were susceptible to HIV1 strains (positive RT assay, immunocapture, immunofluorescence, cytopathic effects, positive hybridization in DOT blot and PCR), 6 clones did not replicate any viral strain used in this experiment (negative RT assay, negative immunocapture, negative immuno-fluorescence and no hybridization in Dot blot, but positive in PCR). One was negative in RT assay, but positive in immunocapture and positive hybridization in DOT blot and PCR.

CONCLUSION: PCR permits to conclude that all CD4+ human lymphocyte clones are infectible. All these experiments are in favour of the existence of three human CD4+ lymphocytes subpopulations: the first one, permissive, replicates HIV; the second, "non permissive" just integrates HIV proviral DNA, without any early replication; the third, is intermediate, with a partial replication. The differences in HIV susceptibility observed in our clones may not be related to the nature of the viral strain used for infection assays, suggesting the importance of virus host cell interactions in the viral replication level. Permissivity mechanisms are under investigation.

Keywords: AEGIS, Antigens, CD4, HIV, Lymphocyte Subsets, HIV-1, RNA-Directed DNA Polymerase, Virus Replication, HIV Core Protein p24, HIV Infections, CD4-Positive T-Lymphocytes, HIV Antibodies, Polymerase Chain Reaction, Clone Cells, HIV Envelope Protein gp120, HIV Envelope Protein gp41, Antigens, CD, HIV Integrase, HIV Envelope Protein gp160, T-Lymphocyte Subsets, HIV Seropositivity, CD9 antigen, Human, virology, ICA6

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