6th International AIDS Conference


San Francisco, California, USA — June 20-23, 1990

Print this Article


Titer affinity parameter: a new blood marker for monitoring individuals with HIV disease.

Int Conf AIDS 1990 Jun 20-23; 6:338 (abstract no. 1098)
Urnovitz HB, Haight V, Schooley RT, Flynn T, Conant MA, Gottfried TD; Calypte Biomedical Corp., Berkeley, CA, USA

OBJECTIVE: To evaluate the humoral response to both HIV-1 core and envelope proteins as a prognostic marker in HIV-1 disease.

METHOD: A quantitative assay, Fine Titer Analysis (FTA), has been developed to determine more precisely the HIV-1 core and envelope antibody responses. FTA is based on a recombinant core and envelope solid phase enzyme immunoassay. The data are normalized by using the Titer Affinity Parameter (TAP) which is a function of antibody concentration and affinity.

RESULTS: Data are presented on 7 patients from a longitudinal study showing significant changes in antibody activity as determined by TAP. Positive and negative fluctuations in TAP were independent of the CD4 T lymphocyte count. Each patient demonstrated a cycle of reduction in antibody activity followed by a recovery phase regardless of the stage of disease.

CONCLUSION: These results describe increases and decreases in HIV-1 core and envelope antibody responses in asymptomatic seropositive individuals. These cycles may correlate with virus or viral derived product bursts through all stages of HIV infection. Significant bursts of biologically active material should influence the titer and affinity of antiserum with time. A prospective study is in progress correlating TAP with other prognostic markers. FTA may identify critical times for the administration of anti-viral treatment.

Keywords: AEGIS, HIV Infections, HIV-1, CD4 Lymphocyte Count, Biological Markers, CD4-Positive T-Lymphocytes, HIV-1 Reverse Transcriptase, Longitudinal Studies, Immunoenzyme Techniques, Prospective Studies, Human, blood, ICA6KWDaegis,hivinfections,hiv-1,cd4lymphocytecount,biologicalmarkers,cd4-positivet-lymphocytes,hiv-1reversetranscriptase,longitudinalstudies,immunoenzymetechniques,prospectivestudies,human,blood,ica6

900620
1098

Copyright © 1990 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.