Differential kinetics of HIV-1 replication in U-937 clonal derivatives: a model for latency in cells of monocytoid origin.
Int Conf AIDS 1990 Jun 20-23; 6:339 (abstract no. 1102) Boulerice F, Geleziunas R, Lvovich A, Wainberg MA; Jewish General Hospital and McGill University AIDS Centre, Montreal, Quebec, Canada
In this study, single cell clones were obtained from the U-937 cell line by limiting dilution. These cells were studied for their susceptibility to HIV infection. We found that two clones (UC12, UC18) could easily support HIV-1 and HIV-2 replication compared with parental U-937 while another clone (UC11) showed baseline infection throughout a ten week culture period. The difference in susceptibility did not correlate with the expression of surface CD4. First, FACS profiles showed equivalent surface CD4 expression for each cell type as detected by Leu3a mAb reactivity. In addition, relative CD4 mRNA expression in each clone was comparable to that of parental U-937. Finally, the level of binding of radiolabeled HIV-1 did not correlate with higher or lower susceptibility to infection. Blocking experiments using Leu3a mAb totally abrogated infection, suggesting that no other receptors were recognized by HIV. Results obtained by polymerase chain reaction analysis and Southern blotting suggest the existence of factors which regulate HIV replication and/or integration after viral entry. This system represents a good model for the study of HIV latency in cells of monocytic origin and may provide a useful tool for studying the role of cellular factors which restrict or enhance viral replication.
Keywords: AEGIS, HIV-1, Virus Replication, Antigens, CD4, HIV Infections, Kinetics, HIV-2, Monocytes, HIV-1 Reverse Transcriptase, Cell Line, Antibodies, Monoclonal, pharmacokinetics, virology, ICA6
