AEGiS-07IAC: Dual tropism of HIV-1 in chimpanzee lymphocytes and monocytes.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

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Dual tropism of HIV-1 in chimpanzee lymphocytes and monocytes.

Int Conf AIDS 1991 Jun 16-21; 7:92 (abstract no. M.A.1001)
Kupcu Z, Mannhalter JW, Schaff S, Eder G, Eibl MM; Immuno AG, Vienna, Austria

OBJECTIVE: To study the cellular tropism of HIV-1/IIIB in the chimpanzee.

METHODS: Monocytes were prepared from peripheral blood of chimpanzees by adherence to plastic. Prior to infection with HIV-1 strain IIIB the adherent cells were cultured for 7 days in medium containing M-CSF (100 U/ml) and GM-CSF (40 U/ml). Nonadherent cells (lymphocytes) were stimulated with PHA for 3 days and maintained in medium containing IL-2(20 U/ml). Both monocytes (5x10(4)IU HIV/ml) and lymphocytes (1x10(3) IU HIV/ml) were infected with HIV-1/IIIB, a lymphocyte-tropic strain of HIV-1. Virus replication was monitored by release of HIV-1 into culture supernatants (assessed by antigen ELISA and RT) as well as by electron microscopy.

RESULTS: While release of HIV-1 from infected PHA lymphoblasts was detectable on day 3 and peaked on day 7, only very low levels of HIV-1 were released from infected monocytes. However, if infected monocytes were cocultivated with human PHA blasts, transmission of virus was readily detected. Replication of HIV-1/IIIB in chimpanzee monocytes was further confirmed by the presence of intracellular virus particles as well as by the detection of HIV-1 budding from the surface of these cells.

DISCUSSION AND CONCLUSIONS: Our studies demonstrate that HIV-1/IIIB, which is considered to be predominantly lymphocyte-tropic in humans, exhibits a dual tropism for lymphocytes and monocytes in the chimpanzee.

Keywords: AEGIS, Monocytes, HIV-1, Pan troglodytes, Lymphocytes, Tropism, Virus Replication, HIV Core Protein p24, HIV Infections, HIV, Phytohemagglutinins, HIV Envelope Protein gp120, HIV Antibodies, HIV Long Terminal Repeat, HIV Antigens, Macrophage Colony-Stimulating Factor, HIV Seropositivity, Granulocyte-Macrophage Colony-Stimulating Factor, Interleukin-2, Animal, Human, virology, genetics, ICA7
910616
MA1001

Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.