AEGiS-07IAC: HIV-1 infection of human fetal macrophages in vivo and in vitro: kinetics of viral gene expression in culture.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

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HIV-1 infection of human fetal macrophages in vivo and in vitro: kinetics of viral gene expression in culture.

Int Conf AIDS 1991 Jun 16-21; 7:97 (abstract no. M.A.1020)
Hatch WC, Burstein Y, Calvelli TA, Rashbaum WK, Lyman WD; Albert Einstein Coll. Med., NY, NY, USA

OBJECTIVE: To compare the kinetics of HIV-1 gene expression in human fetal macrophages (HFM) infected in vivo with the kinetics of infection of normal HFM in vitro using different HIV-1 isolates.

METHODS: HFM from fetal livers were obtained from abortuses of HIV-1 seropositive and seronegative women. The cells were cultured using standard conditions and analyzed for phenotype by flow cytometry and non-specific esterase staining. HFM from abortuses of HIV-1 seropositive females were monitored for infection by reverse transcriptase (RT) and p24 assays, immunocytochemistry and nucleic acid hybridization. HFM from abortuses of seronegative women were exposed to the RF (L-cytotropic) and JRFL (M-cytotropic) isolates of HIV-1. These cultures were monitored for HIV-1 infection using the same methods.

RESULTS: HFM obtained from HIV-1 seropositive females showed a 3-fold increase in RT activity after 30 days in culture. Similarly, HFM from abortuses of seronegative females incubated with the RF strain showed a 3-fold increase in RT activity after 30 days in culture. In contrast, HFM incubated with the JRFL strain showed a 50-fold increase in RT activity by 14 days in culture.

DISCUSSION AND CONCLUSIONS: The kinetics of HIV-1 expression in cultured HFM infected in vivo were similar to HFM infected in vitro by the L-cytotropic RF isolate of HIV-1. This slow-low expression of HIV-1 may be related to viral latency. In contrast, the expression of viral genes after exposure of HFM to the M-cytotropic isolate (JRFL) demonstrated high-fast kinetics. These data suggest that HIV-1 infection of the human fetus by distinct viral isolates may contribute to the observed differences in post-natal pathology.

Keywords: AEGIS, HIV Infections, HIV-1, Macrophages, RNA-Directed DNA Polymerase, Culture, HIV Seropositivity, Fetus, Human, Female, In Vitro, ethnology, virology, pharmacokinetics, ICA7KWDaegis,hivinfections,hiv-1,macrophages,rna-directeddnapolymerase,culture,hivseropositivity,fetus,human,female,invitro,ethnology,virology,pharmacokinetics,ica7
910616
MA1020

Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.