AEGiS-07IAC: The establishment of a rat cell line continuously producing HIV1.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

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The establishment of a rat cell line continuously producing HIV1.

Int Conf AIDS 1991 Jun 16-21; 7:98 (abstract no. M.A.1027)
Mizrachi Y, Sternas L, Volsky DJ; St. Luke's/Roosevelt Hospital Center, Columbia University, New York, N.Y.

OBJECTIVE: To establish a rodent cell line stably carrying HIV-1 genome and replicating HIV-1 over extended periods of time, as a model system for studying HIV-1 replication in the absence of HIV-1 receptors.

METHODS: A vector carrying an infectious clone of HIV-1 and the neomycin resistance gene was electroporated into various non-human cells. The cells were analyzed for the presence of the HIV-1 genome, HIV-1 replication and for transactivation of the HIV-1 LTR in a transient transactivation assay.

RESULTS: Three subgroups of cells were identified with respect to HIV-1 expression and HIV-1 LTR activation: i) cells which were non-permissive to both HIV-1 LTR transactivation and HIV-1 expression; ii) cells in which the HIV-1 LTR was transactivated but no HIV-1 replication was detected. iii) cells which permitted both the LTR transactivation and HIV-1 replication. The third group included a normal embryonal rat cell line; these cells were capable of carrying the HIV-1 genome for 4 months; at the peak of infection, 5-20% of the infected cells expressed HIV-1 antigens as detected by IF, and HIV-1 production reached 30-36ng of p24 per 1x10(6) cells. HIV-1 expression in these cells declined after 3 months; however, HIV-1 expression could be enhanced 5-7 fold by treatment with sodium butyrate, and to a lesser extent by PMA. Virus released by the persistently infected rat cells was infectious in human T lymphocytes.

CONCLUSIONS: The model system described here will be useful in studies on HIV-1 expression in CD4- cells, identification and characterization of cellular factors needed for HIV-1 infection, and characterization of non-CD4 receptor(s) for HIV-1.

Keywords: AEGIS, HIV Long Terminal Repeat, Cell Line, HIV-1, Virus Replication, Antigens, CD4, Trans-Activation (Genetics), HIV Envelope Protein gp120, HIV Core Protein p24, HIV Antigens, T-Lymphocytes, Rats, Animal, Human, genetics, virology, ICA7KWDaegis,hivlongterminalrepeat,cellline,hiv-1,virusreplication,antigens,cd4,trans-activation(genetics),hivenvelopeproteingp120,hivcoreproteinp24,hivantigens,t-lymphocytes,rats,animal,human,genetics,virology,ica7
910616
MA1027

Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.