AEGiS-07IAC: Cytotoxic activity and HIV sensitivity of intestinal lamina propria lymphocytes.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

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Cytotoxic activity and HIV sensitivity of intestinal lamina propria lymphocytes.

Int Conf AIDS 1991 Jun 16-21; 7:101 (abstract no. M.A.1039)
DiMassimo AM, Placido R, Bach S, Mastino AS, Capobianchi M, Fais S, Pallone F, Colizzi Y; Department of Biology and of Experimental Medicines, II University of Rome, Italy

OBJECTIVE: The role of mucosal immunity in HIV infection has been analyzed at the level of lamina propria lymphocytes (LPL).

METHODS: LPL were isolated from the normal colon-rectal mucosa by enzymatic treatment, and phenotypically characterized by flow cytometry. T cell mitogen (PHA, staphylococcus toxin, IL-2) activated LPL and peripheral blood lymphocytes (PBL) were used as effector cells in a 4 hour chromium release cytotoxic assay. Tumor cell lines either uninfected or HIV-infected were used as targets.

RESULTS: LPL after activation display high cytotoxicity against a variety of tumor cells, and no preferential killing was observed when intestinal derived tumor cell lines were used. Moreover, LPL display cytotoxic activity against the H9/HIV cell line and this cytotoxic activity is mediated by activated T cells. Considering the predominance of CD4+ T cells in the LPL population, their sensitivity to HIV infection has been evaluated using low doses of infectious virus. It has been found that only activated LPL can be infected by HIV and produce infectous virus.

CONCLUSIONS: The colon-rectal lamina propria mucosa may represent a first defense barrier to natural HIV infection, and these data show that LPL easily activated in situ might be infected with low doses of HIV, although they are also able to exert cytotoxic activity.

Keywords: AEGIS, HIV, Lymphocytes, T-Lymphocytes, HIV Infections, Phytohemagglutinins, Interleukin-2, Antigens, CD4, Mitogens, Colon, ICA7KWDaegis,hiv,lymphocytes,t-lymphocytes,hivinfections,phytohemagglutinins,interleukin-2,antigens,cd4,mitogens,colon,ica7
910616
MA1039

Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.