Mapping of neutralizing antibodies on the SIV env glycoprotein.
Int Conf AIDS 1991 Jun 16-21; 7:28 (abstract no. M.A.25) Collignon C, Thiriart C, Langedijk H, Meloen R, Desrosiers R, DeWilde M, Bruck C; SmithKline Beecham Biologicals, Rixensart, Belgium
Several monoclonal antibodies were derived from Balb/c mice immunized with vaccinia expressed SIV gp150. These reagents were characterized for reactivity to different forms of SIV envelope protein and to different SIV isolates using radioimmunoprecipitation and Western Blot (WB). Among these, 2 showed a neutralizing activity. Their reactivity to envelope subframents expressed in E. coli was monitored by enzyme immuno assays (EIA). One of these reagents was shown to recognize an epitope located in the aminoterminal moiety of SIV gp120. The other one was unreactive to these subfragments when tested in EIA or WB and did not react to denatured gp150 in Western Blot, although clearly positive in RIPA. Therefore the target epitope of this neutralizing mAb appears to be conformationnaly determined. The E. coli expressed subfragments of SIV env were furthermore injected into rabbits and one of them encompassing the carboxyterminal half of SIV env gp120 was able to induce a strong neutralizing response in these animals. These results allow us to define at least 3 neutralizing epitopes on SIV env gp120. Further mapping of these epitopes using the pepscan system will be presented.
Keywords: AEGIS, Gene Products, env, SIV, HIV Envelope Protein gp120, Epitopes, Antibodies, Monoclonal, Blotting, Western, Radioimmunoprecipitation Assay, simian immunodeficiency virus gp120, Animal, Mice, Rabbits, ICA7 910616
MA25
