AEGiS-07IAC: Interactions of HIV and HPV in the development of penile cancers.

7th International AIDS Conference


Florence, Italy — June 16-21, 1991

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Interactions of HIV and HPV in the development of penile cancers.

Int Conf AIDS 1991 Jun 16-21; 7:29 (abstract no. M.A.31)
Tornesello ML, Beth-Giraldo E, Galloway DA, McDougall JK, Buonaguro FM, Giraldo G; Natl. Cancer Institute "Fond. Pascale", Naples, Italy

OBJECTIVE: Analysis of the interactions and the role of HIVs and HPVs in the etiopathogenesis of penile cancers from patients of African regions with high prevalence of HIV infections and genital neoplasms.

METHODS: To test the prevalence of HIVs and HPVs, DNAs extracted from Penile Cancers (PC) biopsies were tested by Dot and Southern blot analysis at high (Tm-28) and low (Tm-50) stringency, and by Polymerase Chain Reaction (PCR) focused on the HIV-1 gag region and the HPVs E6/E7 and L1 regions. Furthermore a genomic library has been constructed.

RESULTS: 15 PC samples, tested for the presence of HPV 6, 11, 16, 18, 31, and 33 DNA sequences, are mainly positive at low stringency (TM -50) with an HPV 16/18 DNA probe, suggesting the presence of HPV strains related to them. The presence of HPVs has been also identified by PCR. The HPV present in PC8 has been further characterized by the construction of a genomic library and a partial recstriction map. HIV sequences have not been identified in these PC biopsies by PCR.

CONCLUSIONS: A highly pathogenic HPV or a cofactor such as immunodeficiency and/or circulating HIV-related regulatory factors could determine the high incidence of genital cancers in these regions. DNA sequence analysis of the E6/E7 region of the cloned HPV, the in vitro transforming activity and the role of HIV regulatory genes (particularly tat) are the current ongoing approaches. The specific results will be discussed.

Keywords: AEGIS, Papillomavirus, Human, Penile Neoplasms, HIV, Blotting, Southern, Polymerase Chain Reaction, Sequence Analysis, DNA, Male, In Vitro, Human, ICA7KWDaegis,papillomavirus,human,penileneoplasms,hiv,blotting,southern,polymerasechainreaction,sequenceanalysis,dna,male,invitro,human,ica7
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Copyright © 1991 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.