Induction of anti HIV antibodies by immunization with monoclonal anti-idiotypes.
Int Conf AIDS 1991 Jun 16-21; 7:38 (abstract no. M.A.66) Zaghouani H, Bona C; Department of Microbiology, Mount Sinai School of Medicine, New York USA
Anti-idiotypes which possess the internal image of an antigen can induce protective humoral immunity toward bacterial, viral and parasitic infections. Herein, we demonstrate antigen mimicry by monoclonal anti-idiotypes to a distinct epitope of the HIV envelope protein which is defined by a synthetic peptide. This peptide corresponding to amino acid residues 503-535 of HIV-1 IIIB gp160 induced antibodies in three mammalian species which interacted with HIV-1 gp120 and inhibited in vitro syncytia formation caused by HIV-1 IIIB and MN isolates. In addition, antibodies to this epitope were purified from the sera of HIV infected humans using the 503-535 peptide. Three monoclonal anti-idiotypes were generated against rabbit anti-gp120 antibodies specific for the 503-535 peptide. These anti-idiotypes recognize an interspecies cross-reactive idiotype expressed mouse, chimpanzee, baboon, rabbit and human anti-gp120 antibodies specific for the 503-535 peptide. The interaction with the cross-reactive idiotypes is inhibited by both synthetic peptide and HIV-1 gp160. Furthermore, rabbits immunized with the monoclonal anti-idiotypes produced antibodies which also bind both HIV-1 gp120 and gp160 and recognized the epitope defined by the 503-535 peptide.
Keywords: AEGIS, HIV Antibodies, Antibodies, Anti-Idiotypic, Immunoglobulin Idiotypes, HIV, HIV-1, Immunization, Epitopes, HIV Envelope Protein gp160, Pan troglodytes, HIV Infections, Antigens, Viral, Animal, Human, In Vitro, Mice, Rabbits, immunology, genetics, ICA7
910616
MA66
