AEGiS-07IAC: HIV-1 load in the blood is inversely related to the level of reverse transcriptase inhibiting (RTI) antibody.

7th International AIDS Conference

Florence, Italy — June 16-21, 1991

HIV-1 load in the blood is inversely related to the level of reverse transcriptase inhibiting (RTI) antibody.

Int Conf AIDS 1991 Jun 16-21; 7:40 (abstract no. M.A.71)
Imagawa DT, Kobata H, Lee M, Ueda M, Javier B, Russo F, Aldrovandi G, Detels R; Harbor-UCLA Medical Center, Torrance, CA

OBJECTIVE: Infectious HIV-1 load in the peripheral blood mononuclear cells (PBMC) and titer of RTI in the corresponding plasma were evaluated in 54 asymptomatic seropositive homosexual men.

METHODS: Limiting dilutions of PBMC were cocultured with PHA- stimulated donor cells in a microculture procedure to quantitate the infectious HIV-1 load. RTI titers in the plasma were determined by purifying IgG with QAE-Sephadex (Pharmacia), diluting and testing for 50% inhibition of HTLV-IIIb RT activity.

RESULTS: There was an inverse relationship between the titer of RTI antibody and the infectious HIV-1 load. In 5 individuals, the HIV-1 level in PBMC was high (greater than or equal to 500 TCID50/10(6) PBMC) and all had low RTI antibody of less than 1:20. In contrast, of 13 men in which HIV-1 was not detected with 2x10(6) PBMC, 10 had RTI titer of greater than or equal to 1:20 (p=less than .01). Results on number of samples with HIV-1 load and RTI titers are summarized below: TABULAR DATA, SEE ABSTRACT VOLUME.

CONCLUSIONS: The above data show that as the RTI antibody titer increases, the viral load decreases. Thus the RTI antibody may have protective effect and needs to be considered in immunotherapy and in development of vaccines.

Keywords: AEGIS, RNA-Directed DNA Polymerase, HIV-1, Viral Load, Antibodies, Reverse Transcriptase Inhibitors, Homosexuality, Immunoglobulin G, Human, Male, blood, ICA7
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