11th International AIDS Conference Vancouver, British Columbia — July 7-12, 1996

Int Conf AIDS 1996 Jul 7-12; 11:24 (abstract no. LB.A.6014)
Zhou HC, Hudson JB, Lee W; Zhuollen Technology Ltd., New Westminster, BC, Canada. Fax: (604) 936-8806.

OBJECTIVE: To evaluate Chinese Herbal Complex X for anti-HIV activity. Materials: Lonicera japonica; Taraxacum monoglicum; Phellodendron Chinese; Coix lacryma-jobi; Astragalus membranaceus; Boswellia carterii, etc.

METHODS: The plaque-assays of evaluation of HCX had carried out in virology laboratory, Division of Medical Microbiology, VGH.

RESULTS: 25-50% HCX was non-toxic and displayed significant anti-Sindbis virus activity; 20-40% HCX had anti-mouse cytomegalovirus activity effects, but less than the former. The tests for anti-HIV activity were carried out in the B.C. Provincial AIDS Lab. (B.C. Centre for Disease Control) in conformity with regulations for HIV culture work. The extract, derived by steam extraction of HCX powder, is considered the starting material (100%) after making isotonic with concentrated saline solution. The extract was centrifuged at 10,000 rpm for 20 minutes in the Sorvall centrifuge to pellet particular material. The clear supernatant was diluted with an equal volume of double-strength medium and filtered. The extract was then diluted with different volumes of medium and mixed (in duplicate) with an equal volume of HIV (10(3) TCD50) in the wells of tissue culture trays. Final concentrations of the extract (100 microliters + 100 microliter virus) were 50, 30, 20,10, 5 and 0%. The mixtures were incubated at 37 degrees C for 60 minutes and 4x10(5) CEM cells, in 2.0 ml complete medium (10% serum) were added to each culture well. At 5 days culture supernat, were removed for p24 antigen assays.

CONCLUSIONS: 1) HCX had significant anti-HIV activity. At concentration 50%, there were no viral CPE, i.e., the virus had been completely destroyed. 2) There was no difference between the extracts derived from 4 different sources.

960707
LBA6014

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