AEGiS-11IAC: Subtype identification and replication capacity of HIV-1 strains isolated in Hungary.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

Print this Article


Subtype identification and replication capacity of HIV-1 strains isolated in Hungary.

Int Conf AIDS 1996 Jul 7-12; 11:56 (abstract no. Mo.A.1005)
Nagy K, Barabas E, Varkonyi V, Horvath A; National Institute of Dermato-Venereology, Budapest, Hungary. Fax: +36 1 134 0566. E-mail: nagkar@bor.sote.hu.

OBJECTIVE: To identify HIV-1 envelope sequence subtype and characterise in vitro replication capacity of HIV strains from infected asymptomatic individuals.

METHODS: DNA samples derived from periferal blood mononuclear cells were analsed by sequencing of HIV-1 env region encoding V3-loop. A 211 nucleotide fragment (7063-7274) of HIV-1 env, containing the entire V3 region and adjacent sequences were amplified by PCR using appropriate oligonucleotide primers. Nucleotide sequence alignment comparison was made to the env region of HXB-2, HIV-1 BRU(LAV-1) and HIV-1 NL43(NY5). Infectious HIVs were isolated by cocultivation and replication capacity of the isolates were determined in 5 permanent cell lines with lymphoid or macrophage origin.

RESULTS: The env sequences derived from individuals infected in Hungary belong to subtype B HIV-1. High similarity (96.9%) to env V3 region of HIV-1 BRU suggests strong subtype association with LAV-1. Replication analysis shows the HIVs isolated are with the rapid/high (R/H or SI) phenotype.

CONCLUSIONS: The delayed occurence and still low rate of the AIDS epidemic in Hungary raise the possibility of the circulation of HIV subtypes with altered replication capacity and pathogenecity compared to those strains of HIV detected in Western Europe and the USA. The env-based classification and the biological characterisation of HIV subtypes and knowledge of their global incidence and prevalance help further understand geographical distribution and functional significance of diversity in relation to transmission and pathogenesis of HIV. (Supported partly by the National AIDS Committee under #4.1.95)

Keywords: AEGIS, HIV-1, Virus Replication, Polymerase Chain Reaction, DNA Primers, Hungary, Europe, In Vitro, virology, ICA11KWDaegis,hiv-1,virusreplication,polymerasechainreaction,dnaprimers,hungary,europe,invitro,virology,ica11

960707
MoA1005

Copyright © 1996 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.