AEGiS-11IAC: Evaluation of anti-HIV-1 reverse-transcriptase (RT) antibody titer in HIV-infected subjects.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Evaluation of anti-HIV-1 reverse-transcriptase (RT) antibody titer in HIV-infected subjects.

Int Conf AIDS 1996 Jul 7-12; 11:58 (abstract no. Mo.A.1014)
Meguro T, Yamazaki S, Takayama S, Shiga K, Takil M, Ito H, Sadamoto S, Koyanagi H, Kakishima H, Yamada K; Dept. of Pediatrics, Institute of Medical Science Division of Research Laboratory, St. Marianna Univ. School Med, Yokohama, Japan. Fax: 045-366-1190.

We tried to study on the antibody titer against reverse-transcriptase (RT) of HIV and its clinical significance in 10 cases of HIV-infected hemophiliac patients, 4 cases of HIV-infected homo/heterosexual subjects and 51 uninfected volunteers.

METHODS: Using recombinant RT antigen of HIV-1, RT antibody in plasma or sear was assayed with automated chemiluminescent enzyme immunoassay (CLEA) which was developed by Riken Co. as AL-1000. Titer of anti-HIV-1 RT antibody was expressed by the following formula: individual level of CS/mean level of CS in uninfected volunteers.

RESULTS: The strength of chemiluminescence (CS) was assayed by CLEIA in the samples of HIV uninfected volunteers and HIV-positive subjects, being the mean values 852 plus or minus 486 (255 approximately CS and 1.02X105 plus or minus 1.26X105 (1561 approximately 5.08 X105) CS respectively. The anti-HIV-1 RT antibody titer in AC (N=68), ARC (n=12) and AIDS (n=11) were 70.6 plus or minus 77.3, 18.1 plus or minus 6.0 and 11.5 plus or minus 5.7 respectively. The titers of anti-HIV-1 RT antibody in both ARC and AIDS were significantly lower than AC(p is less than 0.001). On the other hand, anti-HIV-1 RT antibody titer in the long term non-progressors was higher than 100. The correlations were significant in the following relationships between the titers of anti-HIV-1 RT antibody and counts of CD4+ lymphocyte (r=0.319, n=79), HIV-RNA copies in plasma (r=0.258, n=84), and anti-MN antibody titers (r=0.773, n=65,p is less than 0.01).

CONCLUSION: Levels of Anti-HIV-1 RT antibody titer corresponded with other surrogate markers and clinical progression. Reduction of anti-HIV-1 RT antibody titer was observed earlier than the decrease of CD4+ lymphocyte counts during the progression of HIV-infected subjects. These results suggested that the assay of anti-HIV-1 RT antibody titer would be significant for the evaluation of clinical progression in HIV-infected subjects.

Keywords: AEGIS, HIV-1 Reverse Transcriptase, HIV, HIV-1, RNA-Directed DNA Polymerase, HIV Infections, Acquired Immunodeficiency Syndrome, HIV Antibodies, CD4 Lymphocyte Count, Reverse Transcriptase Inhibitors, AIDS-Related Complex, HIV Core Protein p24, Evaluation Studies, HIV Antigens, HIV Seropositivity, Immunoenzyme Techniques, Human, nursing, ICA11

960707
MoA1014

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