AEGiS-11IAC: Different viral variants in chromosomic and extra-chromosomic viral DNA in an in vitro superinfection with HIV-1.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Different viral variants in chromosomic and extra-chromosomic viral DNA in an in vitro superinfection with HIV-1.

Int Conf AIDS 1996 Jul 7-12; 11:58 (abstract no. Mo.A.1018)
Marquina S, Gomez CM, Galvan V, Libonatti O, Rabinovich RD; National Reference Centre for AIDS, Buenos Aires, Argentina. Fax: 54 1 962 5404.

OBJECTIVE: to study the viral DNA in the chromosomic and extrachromosomic fraction and the viral progenie in a superinfection, with another variant, of a cell line persistently infected with an HIV-1 strain.

METHODS: The superinfection system consisted in the H9HTLVIIIB cell line persistently infected, and the superinfectant strain was the HIV-1MN. The chromosomic and extrachromosomic DNA was separated by means of a precipitation technique described by Hirt. In these fractions and in DNA extracted from infected cells with the viral progenie of the superinfected cell lines, C2V3 region of the gp120 env gene was amplified by PCR. To distinguish between the genomes of the two strains, PCRs products were cut with Mnl1 and BsaJ1 restriction enzymes.

RESULTS: Forty-eight hours post superinfection, both viruses, the persistent and the superinfectant strains, were found in the chromosomic DNA fraction. Interestingly, the superinfectant strain was mainly observed in the extrachromosomic viral DNA. However, the viral product obtained from the superinfection was HIV-1HTLVIIIB.

CONCLUSIONS: We report a direct evidence concerning the origin of the extrachromosomic viral DNA which seems to be produced by reinfection. At least in short time, the virus production comes from the first infectant viral strain. Then, the role of the viral extrachromosomic DNA in the generation of variability should be further studied.

Keywords: AEGIS, HIV-1, DNA, Viral, Superinfection, Variation (Genetics), Polymerase Chain Reaction, Cell Line, Genes, env, Anti-HIV Agents, In Vitro, virology, genetics, ICA11KWDaegis,hiv-1,dna,viral,superinfection,variation(genetics),polymerasechainreaction,cellline,genes,env,anti-hivagents,invitro,virology,genetics,ica11

960707
MoA1018

Copyright © 1996 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.