AEGiS-11IAC: A macromolecular multicomponent peptide vaccine candidate induces mucosal immunity against HIV-1.

11th International AIDS Conference

Vancouver, British Columbia — July 7-12, 1996

A macromolecular multicomponent peptide vaccine candidate induces mucosal immunity against HIV-1.

Int Conf AIDS 1996 Jul 7-12; 11:6 (abstract no. Mo.A.152)
Hiroki B, Asakura Y, Tsuji T, Xin KQ, Sasaki S, Hamajima K, Fukushima J, Kawamoto S, Fujita K, Okuda K; Yokohama City University School of Medicine, Yokohama, Japan. Fax: 81-45-785-8438.

OBJECTIVE: To induce HIV-1-specific mucosal IgA antibody with neutralizing activity is of importance for development of HIV vaccine. Induction of secretory IgA antibody against HIV-1 was studied by oral, rectal and vaginal immunization with a new synthetic peptide vaccine candidate (VC1). Method: VC1 was composed of peptides from several third hypervariable regions (V3), a CD4 binding site (helper T cell epitope) and a Gag region of HIV-1. VC1 + cholera toxin (CT) were administered into BALB/c mice with oral, rectal and vaginal route. Fecal extract solutions or vaginal washings were collected at a week after each immunization. HIV-1-specific IgA antibody titers were determined using ELISA assay. Delayed type hypersensitivity (DTH) and cytotoxic T lymphocyte (CTL) were also examined using footpad swelling test, 51Cr release assay, respectively.

RESULTS: Oral, rectal and vaginal immunization with VC1+CT elicited high titer of HIV-1-specific secretory IgA antibody (1:27-9). Secretory IgA antibody induced by oral immunization neutralized not only laboratory strains but also primary isolates. Rectal immunization elicited fecal IgA antibody (1:29) and vaginal IgA antibody (1:27). Vaginal immunization elicited fecal IgA antibody (1:29) and vaginal IgA antibody (1:29). Rectal and vaginal administration of VC1+CT also induced HIV-1-specific DTH and CTL.

CONCLUSIONS: Mucosal immunization with VC1+CT induced secretory IgA antibody with neutralization of HIV-1 and cell-mediated immunity.

Keywords: AEGIS, HIV-1, Immunity, Mucosal, AIDS Vaccines, Vaccines, Subunit, T-Lymphocytes, Cytotoxic, Vaccines, Synthetic, Immunoglobulin A, Secretory, Antigens, CD4, Immunoglobulin A, Immunity, Cellular, Cholera Toxin, Mucous Membrane, Peptides, Immunization, Animal, Mice, Female, immunology, ICA11

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MoA152