AEGiS-11IAC: Use of synthetic peptides for differentiation between HIV 1 group M and group O, and for classification of group M (A - E) and group O strains into subtypes.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Use of synthetic peptides for differentiation between HIV 1 group M and group O, and for classification of group M (A - E) and group O strains into subtypes.

Int Conf AIDS 1996 Jul 7-12; 11:8 (abstract no. Mo.A.163)
Brust S, Knapp S, Gurtler LG; Behringwerke AG, Marburg, Germany. Fax: 49-6421-39-4680. E-mail: brust1@msmbwma.marburg.hoechst-ag.dbp.de.

OBJECTIVE: To develop synthetic peptide based immuno assays to differentiate between HIV 1 group M and group O infections. Furthermore to establish synthetic peptide based immuno assays suitable to classify Anti-HIV 1 positive sera into subtype A - E of group M and into different subtypes of group O.

METHODS: Relevant epitopes from the transmembrane protein gp41 and from the V3 loop region of gp120 of different HIV isolates were identified. Synthetic peptides corresponding to these epitopes were synthesized and coated on micro-titration plates. Sera from different geographic regions (Europe, East & West Africa and Asia) were analyzed by using these plates as solid phases for the immuno assays.

RESULTS: The designated combination of immuno assays based on different synthetic peptides was suitable to screen for HIV 1 group O specimen. 21 HIV 1 group O infected individuals could be identified and, in part, confirmed by PCR. Within this panel different reactivity pattern with synthetic peptides derived from different group O isolates were observed. Within the panel of HIV 1 group M classified sera from West Africa all relevant subtypes (A - E) could be detected. Screening Anti-HIV 1 positive specimen from Europe and Asia show some strong reactivities with subtype B and E, additionally other subtypes. Some of these serologically identified samples were confirmed to be close to subtype E consensus sequence by PCR amplified sequences.

CONCLUSION: The newly developed synthetic peptide based immuno assays allows the serological differentiation and subtyping of HIV 1 group M and group O specimen, which is most important to know doing and interpretating reliable PCR analysis from newborns delivered from mothers infected in various geographic regions.

Keywords: AEGIS, HIV-1, Peptides, Consensus Sequence, Polymerase Chain Reaction, Africa, Europe, Asia, Infant, Newborn, classification, genetics, ICA11KWDaegis,hiv-1,peptides,consensussequence,polymerasechainreaction,africa,europe,asia,infant,newborn,classification,genetics,ica11

960707
MoA163

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