AEGiS-11IAC: Heat shock specific amplification of HIV provirus.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Heat shock specific amplification of HIV provirus.

Int Conf AIDS 1996 Jul 7-12; 11:438 (abstract no. Pub.A.1004)
Angelika V, Kozlova AV, Abelian AV, Yolov AA, Kornilayeva GV, Karamov EV; A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia. Fax: 939-31-81.

OBJECTIVE: To evaluate viral DNA load in acutely HIV-infected cells under a heat shock (HS, HS-cells).

METHODS: HS of HIV RF infected H9 lymphoblastoid line cells at 41.5 C for 1 h followed by propagation in presence of selected azidothyimidne concentration in order to prevent reinfection. HIV provirus copy number has been determined by quantitative PCR in relation to cell DNA marker copy number.

RESULTS: At the first point HIV provirus copy number in HS-cells is three times as high as in untreated control cells. While being stable during 24 h, difference raises to four times at 48 h point and reaches to five times at 96 h point. Infection of previously heat treated cells does not lead to amplification effect.

CONCLUSIONS: It is shown that the HS of HIV-infected H9 cells leads in vitro to HIV provirus amplification. While origin of the effect is constrained to the HS, it is self-maintained afterwards. (table: see text).

Keywords: AEGIS, Proviruses, HIV, Shock, HIV-1, HIV Infections, Heat, Polymerase Chain Reaction, HIV Seropositivity, HIV Core Protein p24, HIV Long Terminal Repeat, HIV Seroprevalence, Viral Load, HIV-1 Reverse Transcriptase, In Vitro, genetics, ICA11KWDaegis,proviruses,hiv,shock,hiv-1,hivinfections,heat,polymerasechainreaction,hivseropositivity,hivcoreproteinp24,hivlongterminalrepeat,hivseroprevalence,viralload,hiv-1reversetranscriptase,invitro,genetics,ica11

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PubA1004

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