AEGiS-11IAC: Evaluation of particle agglutination test (serodia) for determination of single and dual HIV-1/2 infections in Bombay.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Evaluation of particle agglutination test (serodia) for determination of single and dual HIV-1/2 infections in Bombay.

Int Conf AIDS 1996 Jul 7-12; 11:439 (abstract no. Pub.A.1007)
Gharpure H, Godse C, Hira S, Kurimura T; Department of Infectious Diseases, MGM Medical College, New Bombay, India. Fax: 91-22-444 0378.

OBJECTIVE: To evaluate efficiency of particle agglutination test (Serodia) to differentiate single and dual HIV-1/2 infections.

METHODS: One hundred and four consecutive patients attending HIV/AIDS referral outpatient service at MGM Hospital in New Bombay were recruited in the study. Serum samples were tested by ELISA (EIAgen HIV 1-2 Kit, Clone System). The reactive samples on ELISA were tested for single or dual HIV-1 &/or 2 using Serodia particle agglutination assay (Fujirebio). Western blot test using new LAVBLOT-I and new LAVBLOT-II (Pasteur) were done for confirmation and interpreted as per WHO/CDC guidelines.

RESULTS: (table: see text)

CONCLUSIONS: Serodia particle agglutination assay had total agreement with ELISA. The single and dual infections determined with Serodia were in agreement with Western blot in all but one sample. The prevalence of HIV-1 (68.4%), HIV-1 & 2 (22.8%) and HIV-2 (8.8%) was observed.

Keywords: AEGIS, Agglutination Tests, Evaluation Studies, HIV-2, Blotting, Western, HIV-1, Enzyme-Linked Immunosorbent Assay, Single Person, Retroviridae Infections, HIV Infections, HIV Seropositivity, Human, nursing, methods, ICA11KWDaegis,agglutinationtests,evaluationstudies,hiv-2,blotting,western,hiv-1,enzyme-linkedimmunosorbentassay,singleperson,retroviridaeinfections,hivinfections,hivseropositivity,human,nursing,methods,ica11

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PubA1007

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