AEGiS-11IAC: Replicative and cytopathic characteristic of AZT-resistant HIV-1 strains.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Replicative and cytopathic characteristic of AZT-resistant HIV-1 strains.

Int Conf AIDS 1996 Jul 7-12; 11:440 (abstract no. Pub.A.1015)
Selimova LM, Pokrovsky VV; The D.I. Ivanovsky Institute of Virology, Moscow, Russia. Fax: 095-1902867. E-mail: nick@nasel.msk.ru.

OBJECTIVE: To revealed the replicative and the cytopathic capacity of AZT-resistant HIV-1 strains isolated from individuals after AZT therapy.

METHODS: HIV-1 strains MS-1974 and MS-1978 were grown in Jurkat-tat, MT-4, CEM, and U-937 cells lines. Viral activity was determined by WB, syncytium-inducing capacity and IF. The antiviral activity was examined in Jurkat-tat cells in the presence of appropriate AZT concentrations.

RESULTS: Before it had been revealed MS-1974 and MS-1978 strains replicated in Jurkat-tat cells and formed syncytia after acute infection. Subsequent study replicative capacity of these viruses showed that both viruses grew in MT-4 and U-937 cells during consecutive passages, but only transient replication pattern was defined in CEM cells. Replicative and cytopathic activities were higher in Jurkat-tat cells for both viruses. Analysis of AZT sensivity showed ED50 was 0,1 uM for MS-1974 and 1uM for MS-1978. Study of the replicative and cytopathic activity with AZT (4 uM) during the 4 serial passages showed following results. There was observed unessential (10%) rise of cells viability, reduction in syncytia formation (5 times) and reduction in the intra- and the extracellular antigen expression (1,3 and 2 times after the 4-th passage respectively) in case of MS-1974 strain. Cultivation of MS-1978 strain was characterised by reduction in cells viability, rise of syncytia formation (2 times) and reduction in intra- and extracellular antigen expression (1,3 and 2 times respectively). Analysis of the strains sensitivity to the AZT showed that ED50 elevated to 0,5 uM for MS-1974 and to greater than 1 uM for MS-1978.

CONCLUSIONS: This results demonstrate that AZT-resistant HIV-1 MS-1974 and MS-1978 strains have biological slow/low-3 phenotype. Replication of AZT-resistant strains in the presence of this drug was accompanied by the lowering of virus antigen accumulation in infected cells.

Keywords: AEGIS, Zidovudine, HIV-1, Virus Replication, Reverse Transcriptase Inhibitors, HIV-1 Reverse Transcriptase, Giant Cells, Antiviral Agents, Jurkat Cells, HIV Protease Inhibitors, Cell Line, Human, virology, ICA11KWDaegis,zidovudine,hiv-1,virusreplication,reversetranscriptaseinhibitors,hiv-1reversetranscriptase,giantcells,antiviralagents,jurkatcells,hivproteaseinhibitors,cellline,human,virology,ica11

960707
PubA1015

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