AEGiS-11IAC: Cell genome stabilization by yeast RNA-tilorone complex in in vitro HIV-infected cells.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Cell genome stabilization by yeast RNA-tilorone complex in in vitro HIV-infected cells.

Int Conf AIDS 1996 Jul 7-12; 11:441 (abstract no. Pub.A.1022)
Karpov AV, Antonenko SV, Barbasheva YV, Spivak NY; D. Zabolotny Institute of Microbiology & Virology, National Academy of Sciences of Ukraine, Kiev, Ukraine. Fax: 380 (044)266-23-79.

OBJECTIVE: We have earlier detected interferonogenic and immunomodulating effects of yeast RNA-tilorone molecular complexes (MC). We have also showed inhibition of in vitro HIV replication due to this preparation Optimization of conditions necessary for MC fawarable action is of great interest.

METHODS: Investigation of anti-HIV activity has been made using models of acute and chronic infections (cells lines U-937 and H-9 respectively, infected by HIV-1/IIIB). Activities of reparative and replicative cell DNA synthesis have been determined by 3H-thymidine incorporation. An MC has been administrated into cell cultures 6 hours before HIV infection; its development has been determined by CPE and p24 antigen accumulation (ELISA).

RESULTS: Preliminary treatment of U-937 cells by MC preparation with components molar ratio 1:10 (tilorone/RNA pho sphate) at a dose 0.01mg per 106 cells has shown cell genome stabilization (from data reparative and replicative DNA synthesis). Such a situation has increased cell stability against HIV cytodestructive effect as well as cell viability. The HIV expression level has been decreased up to 80% comparing to control cultures. Some similar data are also obtained with H-9 cells.

CONCLUSIONS: The data presented suggest the further MC study in HIV-depression experiment to be promising.

Keywords: AEGIS, HIV, Tilorone, HIV-1, Virus Replication, RNA, HIV Infections, HIV Core Protein p24, Yeasts, RNA, Fungal, Anti-HIV Agents, Interferon Inducers, Cell Line, HIV Antibodies, HIV Protease Inhibitors, RNA, Viral, DNA Replication, RNA, Double-Stranded, In Vitro, pathogenicity, virology, genetics, ICA11

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PubA1022

Copyright © 1996 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.