AEGiS-11IAC: Interleukin(IL)-12 induces IL-10 expression in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Interleukin(IL)-12 induces IL-10 expression in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals.

Int Conf AIDS 1996 Jul 7-12; 11:442 (abstract no. Pub.A.1027)
Daftarian PM, Kumar A, Diaz-Mitoma F; Dept. of Micro. & Immuno., University of Ottawa, Ottawa, Canada. Fax: 613-738-4819.

OBJECTIVE: To evaluate the effect of IL-12 on IL-10 production of PBMC from HIV-infected individuals.

METHODS: PBMC from twenty seven HIV+ and five HIV- individuals were studied for the in vitro effect of IL-12 on IL-10 production. PBMC were cocultured with recall antigens or PHA or media alone with or without IL-12. IL-10 and TNF-alpha were measured after 48 (PHA) and 7 days (recall antigens). Increasing amount of IL-12 was added to PBMC from HIV+ and HIV- people and IL-10 /TNF-alpha were measured at different time points.

RESULTS: Upon IL-12 treatment, IL-10 was increased in 16 out of 27 HIV-infected patients. Increase IL-12 was most effective for IL-10 induction when it was used with PHA. TNF-alpha was elevated in all (27/27)HIV+ individuals as it was increased in HIV- controls (5/5). When IL-12 was used with PHA, IL-10 production reached its peak after 48 hours. TNF-alpha was enhanced in the absence of PHA and reached its peak in 48 hours.

CONCLUSION: We have previously shown that IL-12 enhances IL-10 expression in human CD4 + as well as CD8+ T cells. Here, we report that in a majority of HIV-infected patients IL-12 increases the IL-10 production by PBMC. Since IL-12 induces the expression of TNF-alpha, the inducer of IL-10 from monocytes, the IL-10 induction of IL-12 might be direct through T cells and/or indirect through monocytes.

Keywords: AEGIS, Interleukin-10, HIV, Interleukin-12, Lymphokines, Tumor Necrosis Factor, Interleukin-2, Phytohemagglutinins, HIV Infections, Monocytes, T-Lymphocytes, HIV-1, Interferon Type II, lymphocyte proliferation inhibitory factor, Human, In Vitro, ICA11

960707
PubA1027

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