AEGiS-11IAC: Interaction of HIV-*1 and human herpes simplex virus (HSV)in mixed infection.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Interaction of HIV-*1 and human herpes simplex virus (HSV)in mixed infection.

Int Conf AIDS 1996 Jul 7-12; 11:442 (abstract no. Pub.A.1029)
Barbasheva YV, Antonenko SV, Gryn YV, Shcherbinska AM; Research Institute of Epidemiology & Infectious Diseases, Kiev, Ukraine. Fax: 044-277-24-00.

OBJECTIVE: To study some events concerning HIV-1 and HSV interaction in in vitro and in vivo mixed infection.

METHODS: The titers of HIV and HSV antibody have been determined in samples of 44 HIV-positive patients. The levels of HIV-1 (p24) and HSV antigens in cell supernatants have been made using ELISA. Some biological properties of HIV-1 isolates have been made by indirect immunofluorescence method, reverse transcriptase reaction, PCR, light and electron microscopy.

RESULTS: High HSV antibody titers (greater than 1:3200) have been detected in 63% of HIV-infected patients carrying anti-HIV antibodies against all the HIV proteins (immunoblots); it is 1,5 times higher comparing to HIV-infected patients having only anti-gag (p15/17,p24 and p55) antibodies. HSV antigens have been shown to be 80% higher in patients of the first group comparing to those of the second one. The cell cultures with mixed HIV-1 of clinical isolates and HSV (types 1 and 2) infection have shown increased CPE (syncytia formation, cytodestruction) and 1,5 - 2,1 times higher virus antigens production. Comparative analysis of biological patterns of paired HIV-1 isolates from 4 HIV-positive patients in the periods of remission (primary isolates) and manifestation (secondary isolates) of HSV infection shown the secondary isolates to possess significantly higher infective, replicative and cytocide activity.

CONCLUSIONS: The data obtained prove HIV-1 and HSV infection to activate each other in in vitro and in vivo mixed infection.

Keywords: AEGIS, HIV-1, Simplexvirus, HIV Infections, HIV Antibodies, HIV, HIV Seropositivity, Drug Interactions, RNA-Directed DNA Polymerase, HIV Seronegativity, Antigens, Viral, Fluorescent Antibody Technique, Indirect, Human, In Vitro, immunology, ICA11KWDaegis,hiv-1,simplexvirus,hivinfections,hivantibodies,hiv,hivseropositivity,druginteractions,rna-directeddnapolymerase,hivseronegativity,antigens,viral,fluorescentantibodytechnique,indirect,human,invitro,immunology,ica11

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PubA1029

Copyright © 1996 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.