AEGiS-11IAC: Sensitivity of polymerase chain reaction (PCR) in cerebrospinal fluid in the diagnosis of cerebral toxoplamosis (CT) in AIDS.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Sensitivity of polymerase chain reaction (PCR) in cerebrospinal fluid in the diagnosis of cerebral toxoplamosis (CT) in AIDS.

Int Conf AIDS 1996 Jul 7-12; 11:222 (abstract no. Th.B.181)
Rodriguez JC, Mora A, Blazquez JC, Escolano CM, Royo G, Martin-Hidalgo A; Servicio de Medicina Interna, Hospital General Universitario de Elche, Spain. Fax: 96-6606108.

OBJECTIVE: To evaluate the efficacy of PCR to detect T. gondii-DNA in cerebrospinal fluid (CSF) from AIDS patients with CT.

METHODS: Retrospective study. 219 samples of CSF were analysed. Group I: 16 samples of CSF from HIV-infection patients with verified CT by finding at CAT-scan and response at treatment, 5 patients were being treated. Group II: 70 samples from immunocompetent subjects with latent toxoplasmosis infection. Group III: 70 samples from immunocompetent patients without latent toxoplasmosis infection. Group IV: 63 HIV-infected patients without CT. PCR amplifications were performed using pairs of primers designed from B1 gene of T. gondii. The reaction was carried through 40 cycles, each consisting of 60 s at 94C, 90 s at 55C and 60 s at 72C. A B1 gene oligonucleotide was used as probe. A chemiluminiscent Dot blot procedure was used to detect hybridized probe. Results were imaged on X-ray film.

RESULTS: (table: see text)

CONCLUSIONS: PCR technique provides a safe and useful method for the diagnosis of CT in HIV-infected patients. The sensitivity is better if patient is not treated yet. The assay incorporates chemiluminiscent probes that make the application of this technology feasible in clinical microbiology laboratories.

Keywords: AEGIS, Sensitivity and Specificity, Acquired Immunodeficiency Syndrome, Polymerase Chain Reaction, Toxoplasmosis, Tomography, X-Ray Computed, HIV, Retrospective Studies, HIV-1, Cats, Animal, Human, diagnosis, cerebrospinal fluid, radiography, ICA11KWDaegis,sensitivityandspecificity,acquiredimmunodeficiencysyndrome,polymerasechainreaction,toxoplasmosis,tomography,x-raycomputed,hiv,retrospectivestudies,hiv-1,cats,animal,human,diagnosis,cerebrospinalfluid,radiography,ica11

960707
ThB181

Copyright © 1996 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.