AEGiS-11IAC: HIV and SIV down-regulate their own infectivity potential through virion surface sialylation.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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HIV and SIV down-regulate their own infectivity potential through virion surface sialylation.

Int Conf AIDS 1996 Jul 7-12; 11:217 (abstract no. Tu.A.141)
Nagai Y, Hu H, Moriya C, Xin X, Hasan M, Shioda T; Institute of Medical Science, Univ. of Tokyo, Tokyo, Japan. Fax: +81-3-5449-5409. E-mail: ynagai@hgc.ims.u-tokyo.ac.jp.

OBJECTIVE: To learn the significance of heavy glycosylation and sialylation of virion surface for the infectivity and cytopathogenicity of HIV-1 and other human and non-human lentiviruses.

METHODS: T cell line tropic HIV-1 strains SF2 and NL43 were treated in vitro or in culture with neuraminidase and their replication kinetics and cytopathogenicity were examined. Effect of inhibitors of mannosidase I (deoxymannojirimycin) and mannosidase II (swansonine) on the viral replication was also examined. Similar studies were extended to the macrophage tropic strain SF162 and primary isolates of HIV-1 as well as HIV-2 strain GH123 and SIV mac strain 239.

RESULTS: Enzymatic desialylation of HIV-1 strain NL43 and SF2 immediately resulted in augmentation of the virus interaction with host cells in a fashion likely undescribed before and led to remarkably enhanced viral replication and cytopathogenicity. Well complementing results came from studies with the inhibitors of mannosidase I and mannosidase II, demonstrating that these inhibitors cause strikingly similar enhancement of HIV-1 replication and cytopathogenicity as found with neuraminidase. Enhancement of infection by neuraminidase was also found with HIV-1 strain SF162 and primary isolates as well as HIV-2and SIV.

CONCLUSIONS: The human and non-human primate lentiviruses appear to down-regulate their own infectivity potential through the virion surface sialylation, thereby determining their individual ordinary infection efficiency. Full processing of the viral oligosaccharide chains into the complex type appears to be impedimental, but neither necessary nor indifferent, to the expression of full infetivity.

Keywords: AEGIS, SIV, HIV, Virion, HIV-1, HIV-2, Virus Replication, Lentivirus, Receptors, HIV, HIV Core Protein p24, HIV Antigens, Macrophages, HIV Long-Term Survivors, Neuraminidase, HIV Infections, Human, In Vitro, pathogenicity, virology, ICA11KWDaegis,siv,hiv,virion,hiv-1,hiv-2,virusreplication,lentivirus,receptors,hiv,hivcoreproteinp24,hivantigens,macrophages,hivlong-termsurvivors,neuraminidase,hivinfections,human,invitro,pathogenicity,virology,ica11

960707
TuA141

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