AEGiS-11IAC: Kinetics of HIV-1 transcription in cells of lymphocytes and monocyte-macrophage lineage.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Kinetics of HIV-1 transcription in cells of lymphocytes and monocyte-macrophage lineage.

Int Conf AIDS 1996 Jul 7-12; 11:218 (abstract no. Tu.A.146)
Vellani NN, Mo T, Leung B, Cassol S; B.C. Centre for Excellence in HIV/AIDS, St Pauls Hospital, Vancouver, BC. E-mail: cassol@hivnet.ubc.ca.

OBJECTIVE: To study the Kinetics of HIV-1 transcription in the cells of macrophage-monocyte lineage and lymphocytes. Method: CEM (lymphocytic) and U937 (promonocytic) cells infected with LAV-1 were harvested from O to 20 hours post infection (hpi). Tat, rev and nef transcripts were quantified as copy numbers by the method of RT-Quantitative-Competitive PCR. This method included cDNA synthesis, amplification in the presence of a competitive template, and separation and quantification of the fluorescent PCR products by an Automated Sequencer (ABI).

RESULTS: The transcription pattern of LAV-1 in CEM cells was that of an active infection and in U937 cells was that of a latent-like infection. In both cell lines, all regulatory transcripts, tat, rev and nef were detectable as early as 4 hpi, although the copy numbers of tat and rev were considerably higher in CEM than in U937 cells. In U937, during the early times, the number of transcript copies of nef ranked the highest and tat ranked the lowest. However as the time progressed, the number of nef transcripts declined and the number of tat transcripts increased.

CONCLUSION: Tat and nef transcription displayed opposite Kinetic trends in U937, contrary to that seen in CEM. The predominant expression of nef and/or low expression of tat during the early times in U937 cells may be involved in promoting the latent-like state. Cells of the macrophage-monocyte lineage and lymphocytes are major reservoir in infected individuals. Therefore, studying HIV-1 replication in these cell-types would be of considerable benefit as it may give us a better understanding of HIV replication and its association to the process of AIDS.

Keywords: AEGIS, Monocytes, HIV-1, Macrophages, Lymphocytes, Transcription, Genetic, Virus Replication, Acquired Immunodeficiency Syndrome, Kinetics, Cell Line, U937 Cells, genetics, virology, ICA11KWDaegis,monocytes,hiv-1,macrophages,lymphocytes,transcription,genetic,virusreplication,acquiredimmunodeficiencysyndrome,kinetics,cellline,u937cells,genetics,virology,ica11

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TuA146

Copyright © 1996 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.