AEGiS-11IAC: Antibody reactivities to p53 and HTLV-1 regulatory proteins- Tax, Rex and Tof in HTLV-I-infected people.

11th International AIDS Conference

Vancouver, British Columbia — July 7-12, 1996

Antibody reactivities to p53 and HTLV-1 regulatory proteins- Tax, Rex and Tof in HTLV-I-infected people.

Int Conf AIDS 1996 Jul 7-12; 11:219 (abstract no. Tu.A.160)
Chen YA, Chen SH, Osame M; National Yang-Ming University, Taipei, Taiwan. Fax: (886-2) 8210514. E-mail: Arthur@dns.ym.edu.tw.

OBJECTIVE: To understand the antibody reactivities and interaction between anti-p53, anti-Tof, anti-Rex and anti-Tax antibodies in HTLV-I-infected people.

METHODS: A GST-Tof expression plasmid was constructed and the fusion protein was used for Western blot assay (WB). In addition, WBs with different antigens which including a GST-p53 recombinant protein, a Tax recombinant protein and cell lysate from HeLa cells transfected with Rex-expressing plasmid were also developed for serological analyses.

RESULTS: Four% (2 /50) of HAM/TSP patients and none of 50 asymptomatic carriers (AC) or 50 ATL patients had anti-Tof antibody; while 2% of ACs, 4% of ATL patients and 6% of HAM/TSP patients had anti-p53 antibody. The rate of anti-Rex antibody of HAM/TSP patients (16%) was significantly higher than that of ACs (2%) or ATL patients (0%). Only one HAM/TSP patient had both anti-Rex and anti-Tof antibody.

CONCLUSIONS: This is the first study demonstrating that there is anti-p53, anti-Rex and anti-Tof antibodies in HTLV-I infected people. Correlation analysis showed that anti-p53 antibody did not have any correlation with anti-Tax antibody in HTLV-I-infected people (McNemar's test, p is less than 0.05). However, the correlation between anti-p53 and anti-Rex antibodies or anti-p53 and anti-Tof antibodies can not be ruled out in this study.

Keywords: AEGIS, Human T-lymphotropic virus 1, Gene Products, tax, Paraparesis, Tropical Spastic, Receptors, Interleukin-2, HTLV-I Antibodies, Antibodies, Deltaretrovirus Antibodies, Blotting, Western, Antibodies, Anti-Idiotypic, Autoantibodies, Antibodies, Viral, Recombinant Proteins, Human, ICA11

960707
TuA160