AEGiS-11IAC: Spontaneous lymphocyte proliferation and IL-2 production in HTLV-II-infected Venezuelan Amerindians.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

Print this Article


Spontaneous lymphocyte proliferation and IL-2 production in HTLV-II-infected Venezuelan Amerindians.

Int Conf AIDS 1996 Jul 7-12; 11:220 (abstract no. Tu.A.163)
Baroja ML, Leon-Ponte M, Noya O, Bianco NE, Echeverria de Perez G; Institutes of Immunology, Miami, FL. Fax: 582-672-0371.

OBJECTIVE: To analyze Peripheral Blood Mononuclear Cell (PBMC) responses in a group of HTLV-II-infected Venezuelan Amerindians.

METHODS: Ten (10) HTLV-II-infected and 12 uninfected Guahibo Amerindians (controls) were studied. Fresh PBMC were isolated and cultured in either medium, PHA or IL-2. Lymphocyte proliferation was measured by 3H-thymidine incorporation. Cell cultures for IL-2 production were supplemented with the anti-Tac mAb to block IL-2 receptor and to prevent IL-2 consumption. IL-2 activity was measured using a bioassay on CTLL.

RESULTS: (table: see text)

CONCLUSIONS: Our results showed significant differences in HTLV-II-infected individuals when compared to non-carriers regarding: 1.- Increased Spontaneous Lymphocyte Proliferation (SLP) at days 5 and 7. 2.- Increased spontaneous IL-2 production. The proliferative response of PBMC from the HTLV-II infected group to either PHA or IL-2 (day 3) was similar to controls. However, in two HTLV-II carriers, a correlation was observed between the highest SLP at days 5 and 7 and the response to exogenous IL-2. These results suggest a pre-activated T-cell status probably related to the HTLV-II infection.

Keywords: AEGIS, Interleukin-2, HTLV-II Infections, T-Lymphocytes, Receptors, Interleukin-2, Phytohemagglutinins, South America, ICA11KWDaegis,interleukin-2,htlv-iiinfections,t-lymphocytes,receptors,interleukin-2,phytohemagglutinins,southamerica,ica11

960707
TuA163

Copyright © 1996 - International AIDS Society (IAS). Reproduction of this abstract (other than one copy for personal reference) must be cleared through the IAS.