AEGiS-11IAC: Phylogenetic analysis of HIV-1/2 in Cameroon based on the pol and env regions: evidence of mixed-infection.

11th International AIDS Conference


Vancouver, British Columbia — July 7-12, 1996

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Phylogenetic analysis of HIV-1/2 in Cameroon based on the pol and env regions: evidence of mixed-infection.

Int Conf AIDS 1996 Jul 7-12; 11:224 (abstract no. Tu.A.372)
Takehisa J, Zekeng L, Miura T, Ido E, Yamashita M, Mboudjeka I, Gurtler LG, Hayami M, Kaptue L; Institute for Virus Research, Kyoto University, Kyoto, Japan. Fax: 81-75-761-9335. E-mail: juntak@virus.kyoto-u.ac.jp.

OBJECTIVE: To clarify the molecular epidemiology of HIV in Cameroon and to assess the incidence of mixed-infection and recombination.

METHODS: Forty eight HIV (47 HIV-1, one HIV-2) were obtained from Cameroonian AC, ARC and AIDS patients in 1994 and 1995. Part of the pol region suitable for comparing all the groups of HIV-related lentiviruses and part of the HIV-1 env region including the V3 loop were amplified by nested PCR, sequenced, and phylogenetically analyzed.

RESULTS: The majority of Cameroonian HIV-1s belonged to clade A (32), but the rest belonged to various clades: clade B (2), clade C (2), clade D (2), clade E (1), clade F (4) and group O (4). In the pol analysis, two different types were found in four individuals (HIV-2a and HIV-1 clade A, HIV-1 group O and clade A, clades A and C, and clades C and F), suggesting double infections with different HIV-1 subtypes or groups. Moreover, three types of the env sequences (clade A, clade D and highly divergent group O) were found in one person, suggesting a triple infection. Concerning the phylogenetic topology of HIV-1 based on the pol region, group M branched into three subgroups alpha, beta and gamma at an early stage. Subgroup alpha mainly consists of African isolates (clades A, E and G). Subgroup beta consists of isolates from central Africa, Europe and United States (clades B, D and F). Subgroup gamma contains isolates from central Africa and India (clade C).

CONCLUSIONS: Various mixed-infection such as between different clades of HIV-1 group M, between HIV-1 and HIV-2, and even between HIV-1 groups O and M were confirmed at a rather high frequency (around 10 %) in the analyzed specimens. Some of the data suggested recombination between different clades of HIV-1 group M. In addition, a case of triple infection was the first to be reported. Thus phylogenetic analyses based on the pol and env regions provided a new insight to the understanding of HIV phylogeny.

Keywords: AEGIS, HIV-2, HIV-1, HIV-1 Reverse Transcriptase, AIDS-Related Complex, Acquired Immunodeficiency Syndrome, Phylogeny, Polymerase Chain Reaction, Cameroon, Africa, Central, India, Europe, United States, Human, ICA11KWDaegis,hiv-2,hiv-1,hiv-1reversetranscriptase,aids-relatedcomplex,acquiredimmunodeficiencysyndrome,phylogeny,polymerasechainreaction,cameroon,africa,central,india,europe,unitedstates,human,ica11

960707
TuA372

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